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The map label for this gene is ruvB [H]

Identifier: 220932068

GI number: 220932068

Start: 1324942

End: 1325970

Strand: Reverse

Name: ruvB [H]

Synonym: Hore_12310

Alternate gene names: 220932068

Gene position: 1325970-1324942 (Counterclockwise)

Preceding gene: 220932069

Following gene: 220932067

Centisome position: 51.43

GC content: 40.91

Gene sequence:

>1029_bases
ATGGATTTAGAAAATAGAATTATTTCACCAGAAAAAAAAGAAGATGATATGGCTCTTGACAGGAGTTTAAGGCCTGAAAG
GCTTAATAACTTTATTGGTCAGGAGAAGGTTAAGGAAAAACTCAGTATTTTCATAGAAGCCGCAATTACCAGGCAGGAAC
CCCTTGATCATGTTTTGCTTTATGGCCCCCCCGGTCTGGGTAAAACCACCCTGGCCAATATAATAGCCAATGAACTTAAT
GTTAGCATTCATACTACTTCAGGACCTGCTATTGAACGACCTGGTGACCTGGCCGCTATCTTAACAAATTTACAACCCAA
AGATGTTTTATTTATTGATGAGATTCACCGCTTAAATAAAGTTGTAGAAGAGGTATTATATCCGGCTATGGAAGATTATT
CCCTGGATATAATAATAGGTAAGGGACCTTCTGCCCGTTCAATAAGGTTAGATCTACCTCCATTTACTCTGGTTGGGGCT
ACAACCAGAGCCGGGTTATTAAGTTCGCCGCTCAGGGACAGGTTCGGGGTAATTAACAGACTGGAATTTTATGGTACTGA
TGAACTGGCTGAAATAATAAAACGTTCGGCCAGGGTTCTGGGGATAGAAGTAAATGAAGATGGGGCTGTTGAAATCTCTA
AAAGATCCCGGGGAACCCCCAGAATTGCCAACAGGTTATTGAAGAGGGTCAGGGATTATGCTGAAGTTAAAGCAGATGGT
ATTATAGATACTGAGGTGGTTGATAAGGCCCTGAAGCTGCTTGAAATAGATGAACTGGGGTTAGACCATATTGATCATAA
ACTACTAAAAACAATTATTAAAAAATTTAACGGGGGTCCGGTGGGTTTAAATACCCTTGCTGCCTCTATAAGTGAGGAAA
CAGAGACAATTGAAGATGTTTATGAGCCCTATCTGTTACAGCTTGGTTTTATTGAACGAACCCCGCGGGGTCGGGTTGCA
ACCCTGGCTGCTTACGAACACCTTGATGTAGACTATAAGAATAAGTCGCAATCTCTTTTTAATAATTAA

Upstream 100 bases:

>100_bases
TTGAAGAAGCCTTAAGTAACGCTGATATAAAAAATAATGGCAGCCTTGAAGAAAATATCAAAAAGGCATTAGGGTACCTT
GGTAGTAAGGGGAGTTAGTC

Downstream 100 bases:

>100_bases
TACATGAGTTAATATTGAACTAATTATGGAGGTCTGAAAATGAAGGTTGAGGAATTTGATTATGAACTACCAGAAGAATT
AATAGCCCAGAAGCCTTTAC

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MDLENRIISPEKKEDDMALDRSLRPERLNNFIGQEKVKEKLSIFIEAAITRQEPLDHVLLYGPPGLGKTTLANIIANELN
VSIHTTSGPAIERPGDLAAILTNLQPKDVLFIDEIHRLNKVVEEVLYPAMEDYSLDIIIGKGPSARSIRLDLPPFTLVGA
TTRAGLLSSPLRDRFGVINRLEFYGTDELAEIIKRSARVLGIEVNEDGAVEISKRSRGTPRIANRLLKRVRDYAEVKADG
IIDTEVVDKALKLLEIDELGLDHIDHKLLKTIIKKFNGGPVGLNTLAASISEETETIEDVYEPYLLQLGFIERTPRGRVA
TLAAYEHLDVDYKNKSQSLFNN

Sequences:

>Translated_342_residues
MDLENRIISPEKKEDDMALDRSLRPERLNNFIGQEKVKEKLSIFIEAAITRQEPLDHVLLYGPPGLGKTTLANIIANELN
VSIHTTSGPAIERPGDLAAILTNLQPKDVLFIDEIHRLNKVVEEVLYPAMEDYSLDIIIGKGPSARSIRLDLPPFTLVGA
TTRAGLLSSPLRDRFGVINRLEFYGTDELAEIIKRSARVLGIEVNEDGAVEISKRSRGTPRIANRLLKRVRDYAEVKADG
IIDTEVVDKALKLLEIDELGLDHIDHKLLKTIIKKFNGGPVGLNTLAASISEETETIEDVYEPYLLQLGFIERTPRGRVA
TLAAYEHLDVDYKNKSQSLFNN
>Mature_342_residues
MDLENRIISPEKKEDDMALDRSLRPERLNNFIGQEKVKEKLSIFIEAAITRQEPLDHVLLYGPPGLGKTTLANIIANELN
VSIHTTSGPAIERPGDLAAILTNLQPKDVLFIDEIHRLNKVVEEVLYPAMEDYSLDIIIGKGPSARSIRLDLPPFTLVGA
TTRAGLLSSPLRDRFGVINRLEFYGTDELAEIIKRSARVLGIEVNEDGAVEISKRSRGTPRIANRLLKRVRDYAEVKADG
IIDTEVVDKALKLLEIDELGLDHIDHKLLKTIIKKFNGGPVGLNTLAASISEETETIEDVYEPYLLQLGFIERTPRGRVA
TLAAYEHLDVDYKNKSQSLFNN

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family [H]

Homologues:

Organism=Homo sapiens, GI18426902, Length=199, Percent_Identity=30.6532663316583, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1788167, Length=326, Percent_Identity=61.3496932515337, Blast_Score=429, Evalue=1e-121,
Organism=Escherichia coli, GI1787119, Length=128, Percent_Identity=36.71875, Blast_Score=69, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324111, Length=171, Percent_Identity=30.4093567251462, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- ProDom:   PD005323 [H]

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 38194; Mature: 38194

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLENRIISPEKKEDDMALDRSLRPERLNNFIGQEKVKEKLSIFIEAAITRQEPLDHVLL
CCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
YGPPGLGKTTLANIIANELNVSIHTTSGPAIERPGDLAAILTNLQPKDVLFIDEIHRLNK
ECCCCCCHHHHHHHHHHHHEEEEEECCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHH
VVEEVLYPAMEDYSLDIIIGKGPSARSIRLDLPPFTLVGATTRAGLLSSPLRDRFGVINR
HHHHHHHHHHCCCEEEEEEECCCCCCEEEEECCCEEEECCCHHHHHHHCHHHHHHHHHHH
LEFYGTDELAEIIKRSARVLGIEVNEDGAVEISKRSRGTPRIANRLLKRVRDYAEVKADG
HHHCCCHHHHHHHHCCCEEEEEEECCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCC
IIDTEVVDKALKLLEIDELGLDHIDHKLLKTIIKKFNGGPVGLNTLAASISEETETIEDV
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
YEPYLLQLGFIERTPRGRVATLAAYEHLDVDYKNKSQSLFNN
HHHHHHHHHHHCCCCCCCEEEEEHHHHCCCCCCCCCHHHCCC
>Mature Secondary Structure
MDLENRIISPEKKEDDMALDRSLRPERLNNFIGQEKVKEKLSIFIEAAITRQEPLDHVLL
CCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
YGPPGLGKTTLANIIANELNVSIHTTSGPAIERPGDLAAILTNLQPKDVLFIDEIHRLNK
ECCCCCCHHHHHHHHHHHHEEEEEECCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHH
VVEEVLYPAMEDYSLDIIIGKGPSARSIRLDLPPFTLVGATTRAGLLSSPLRDRFGVINR
HHHHHHHHHHCCCEEEEEEECCCCCCEEEEECCCEEEECCCHHHHHHHCHHHHHHHHHHH
LEFYGTDELAEIIKRSARVLGIEVNEDGAVEISKRSRGTPRIANRLLKRVRDYAEVKADG
HHHCCCHHHHHHHHCCCEEEEEEECCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCC
IIDTEVVDKALKLLEIDELGLDHIDHKLLKTIIKKFNGGPVGLNTLAASISEETETIEDV
CCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
YEPYLLQLGFIERTPRGRVATLAAYEHLDVDYKNKSQSLFNN
HHHHHHHHHHHCCCCCCCEEEEEHHHHCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA