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The map label for this gene is mrpE [H]

Identifier: 220932049

GI number: 220932049

Start: 1304895

End: 1305413

Strand: Reverse

Name: mrpE [H]

Synonym: Hore_12120

Alternate gene names: 220932049

Gene position: 1305413-1304895 (Counterclockwise)

Preceding gene: 220932051

Following gene: 220932048

Centisome position: 50.63

GC content: 35.45

Gene sequence:

>519_bases
ATGAAGAAAAATAAAAAAGGAAGGGTCTTTACTTTTATATTTGTTTTTCTTGTCTGGATGTTATTAACAGCCAGCCCGGG
TAAGGATACTCTTTTACTGGGGATAATTGTTTCCCTGGTATCCAGCTTTCTTTTCAGTGATATGTTATTCAGATTTTCTT
CAAGGACTTATTCCTTTAAAGTATATGTCCGGAAAATATTATTAATGATATTATTTATATTTGTATTTTTTTATGAAGCC
TTTGTTGCCGCTATTAAAGTAGCCCGACATGCTTTTGAAAAGACTCCCAGTTTTTCACCGGGGATTGTCAAAGTAAAGAC
CAGTTTAACCAATGTATCAGCCATTACAATTCTGGCCAATTTGATTACACTGACCCCCGGTACTTTAACCCTTGATTTTG
ATACCAGTGAAAGGTCCTTTTATATACACTGGATAGATGTACACACTGAAGAAGAAGCTGAAACCAGAAAGGCCTTAATT
GAGAGGTTTGAAAGGTGGCTTGGAGTGATTTTTAGATGA

Upstream 100 bases:

>100_bases
AAAACCATTGTCAAGCTACATCAGGTTATGATAGACTTATGATAGGAATTAAATGATTGTAATAAAGTTACATCCAGATT
TTAAAATGAGGGGTTAAATT

Downstream 100 bases:

>100_bases
TAATATTTATGAGTATTTTTTTATCATTACTGGCAATTATGTGTCTATATCGTGTTATAGTCGGCCCCACTATTCCTGAT
AGGTTGATTGCAGCCGATTC

Product: cation antiporter

Products: NA

Alternate protein names: Mrp complex subunit E; Multiple resistance and pH homeostasis protein E [H]

Number of amino acids: Translated: 172; Mature: 172

Protein sequence:

>172_residues
MKKNKKGRVFTFIFVFLVWMLLTASPGKDTLLLGIIVSLVSSFLFSDMLFRFSSRTYSFKVYVRKILLMILFIFVFFYEA
FVAAIKVARHAFEKTPSFSPGIVKVKTSLTNVSAITILANLITLTPGTLTLDFDTSERSFYIHWIDVHTEEEAETRKALI
ERFERWLGVIFR

Sequences:

>Translated_172_residues
MKKNKKGRVFTFIFVFLVWMLLTASPGKDTLLLGIIVSLVSSFLFSDMLFRFSSRTYSFKVYVRKILLMILFIFVFFYEA
FVAAIKVARHAFEKTPSFSPGIVKVKTSLTNVSAITILANLITLTPGTLTLDFDTSERSFYIHWIDVHTEEEAETRKALI
ERFERWLGVIFR
>Mature_172_residues
MKKNKKGRVFTFIFVFLVWMLLTASPGKDTLLLGIIVSLVSSFLFSDMLFRFSSRTYSFKVYVRKILLMILFIFVFFYEA
FVAAIKVARHAFEKTPSFSPGIVKVKTSLTNVSAITILANLITLTPGTLTLDFDTSERSFYIHWIDVHTEEEAETRKALI
ERFERWLGVIFR

Specific function: Mrp complex is a Na(+)/H(+) antiporter that is considered to be the major Na(+) excretion system in B.subtilis. Has a major role in Na(+) resistance and a minor role in Na(+)- and K(+)-dependent pH homeostasis as compared to TetB. MrpA may be the actual N

COG id: COG1863

COG function: function code P; Multisubunit Na+/H+ antiporter, MnhE subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit E family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002758 [H]

Pfam domain/function: PF01899 MNHE [H]

EC number: NA

Molecular weight: Translated: 19914; Mature: 19914

Theoretical pI: Translated: 10.51; Mature: 10.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNKKGRVFTFIFVFLVWMLLTASPGKDTLLLGIIVSLVSSFLFSDMLFRFSSRTYSFK
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
VYVRKILLMILFIFVFFYEAFVAAIKVARHAFEKTPSFSPGIVKVKTSLTNVSAITILAN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHH
LITLTPGTLTLDFDTSERSFYIHWIDVHTEEEAETRKALIERFERWLGVIFR
HHHCCCCEEEEEECCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKNKKGRVFTFIFVFLVWMLLTASPGKDTLLLGIIVSLVSSFLFSDMLFRFSSRTYSFK
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
VYVRKILLMILFIFVFFYEAFVAAIKVARHAFEKTPSFSPGIVKVKTSLTNVSAITILAN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHH
LITLTPGTLTLDFDTSERSFYIHWIDVHTEEEAETRKALIERFERWLGVIFR
HHHCCCCEEEEEECCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377; 10198001; 11356194 [H]