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The map label for this gene is ytsJ [H]

Identifier: 220931850

GI number: 220931850

Start: 1097051

End: 1098274

Strand: Direct

Name: ytsJ [H]

Synonym: Hore_10070

Alternate gene names: 220931850

Gene position: 1097051-1098274 (Clockwise)

Preceding gene: 220931849

Following gene: 220931852

Centisome position: 42.55

GC content: 42.08

Gene sequence:

>1224_bases
ATGGATTTATATCGGGAAGCATTAAATATGCATAAACAGAAAACCGGTAAAATTAAAGTAGAGTGTAAGGTGGATTTAAA
AGATCAACATGATTTAAGCCTGGCTTATTCTCCCGGGGTGGCCGAACCCTGTCGTCAGATAGCTAAAAATAAAGAGCTTC
AGTATGAATATACTGCTAAAGGGAATCTGGTGGCTGTTGTATCTTCAGGAACAGCAGTACTAGGGTTAGGTGACATAGGG
CCTGAGGCAGCCCTGCCGGTGATGGAAGGTAAGGCTGTTCTTTTTAAGAAGTTTGCCGGTATTGATGCTTTTCCCCTGTG
TGTGGGAAGCAAAGATGTAGATGATATTGTGAACTTTGTAAAGCTTCTAGAACCTACTTTTGCGGGTATCAATCTGGAAG
ATATTGCAGCTCCGGAATGTTTTGAGATTGAAAAACGTTTAAAAAGAGAGACAAATATGGCTATATTCCACGATGACCAG
CATGGAACTGCTATTGTTACCTTAGCAGGTTTAATTAATGCTTTAAAACTGGTAAATAAAGATATAGCTTCAGTTAAAAT
TGTAGTTAATGGGGCCGGGGCATCAGCAACAGCTGTTACTAAACTTTTACTTGAAGCCGGTGCCAGGGAAATTATTGTGT
GTGATAGACTGGGTGCCCTGTACAGTGGCCGGAGTGAGCATATGAATGATGCTAAAATAGAGCTTGCTGAAATAACCAAT
CCAGGGAAAGTAAAGGGGCCCCTTTCTAAAGTAATTAATGGGGCAGATGTATTTATAGGACTTTCAGTCGGTAATATCCT
TACTGGTGATATGGTTAAACTTATGGACAGGGATCCGGTTATTTTTGCTCTGGCCAATCCTACACCCGAGATAGATCCCG
GTCTGGCCAGAGAAGCCGGGGCTGAAATAATTGCTACTGGAAGATCTGATTATGCCAACCAGATAAATAATGTACTGGCA
TTTCCCGGGGTTCTTAGAGGAGCTTTAGATGTCAGGGCTAGAGATATAAATGATAAAATGAAACTGGCAGCAGCCCATGC
TATTGCCAGACTGGCTGAAAAAGAACTCAATAAGGAATACATAATACCGGCTCCCTTTGATTCCAGAGTAGCCCCGGAAG
TGGCAGCAGCAGTAGCTAAAGTGGCTATGGAAACCGGAGTAGCCAGAATTAATATTTCCCCGGAGGAGGTTAAAAGACAT
ACTGAAAAGATAACTGAACTGTAA

Upstream 100 bases:

>100_bases
TGAAAATTTACCCCTGATTGTTGTTAATGATATTTATGGTAATGACCTTTATGAAATAGGACGGAAAAAATATAAAAAAA
TATAACAGGAGATGGTCTAA

Downstream 100 bases:

>100_bases
TATTTTGTTTAATTTAATTTTCAAATAATAAAATTAGGGATAGTCCCCTGTAAGAAGGAATCGAGACCTGTTTGATGATT
TGAAACTGTTATTGACGCTG

Product: Malate dehydrogenase

Products: NA

Alternate protein names: NAD-ME 4 [H]

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MDLYREALNMHKQKTGKIKVECKVDLKDQHDLSLAYSPGVAEPCRQIAKNKELQYEYTAKGNLVAVVSSGTAVLGLGDIG
PEAALPVMEGKAVLFKKFAGIDAFPLCVGSKDVDDIVNFVKLLEPTFAGINLEDIAAPECFEIEKRLKRETNMAIFHDDQ
HGTAIVTLAGLINALKLVNKDIASVKIVVNGAGASATAVTKLLLEAGAREIIVCDRLGALYSGRSEHMNDAKIELAEITN
PGKVKGPLSKVINGADVFIGLSVGNILTGDMVKLMDRDPVIFALANPTPEIDPGLAREAGAEIIATGRSDYANQINNVLA
FPGVLRGALDVRARDINDKMKLAAAHAIARLAEKELNKEYIIPAPFDSRVAPEVAAAVAKVAMETGVARINISPEEVKRH
TEKITEL

Sequences:

>Translated_407_residues
MDLYREALNMHKQKTGKIKVECKVDLKDQHDLSLAYSPGVAEPCRQIAKNKELQYEYTAKGNLVAVVSSGTAVLGLGDIG
PEAALPVMEGKAVLFKKFAGIDAFPLCVGSKDVDDIVNFVKLLEPTFAGINLEDIAAPECFEIEKRLKRETNMAIFHDDQ
HGTAIVTLAGLINALKLVNKDIASVKIVVNGAGASATAVTKLLLEAGAREIIVCDRLGALYSGRSEHMNDAKIELAEITN
PGKVKGPLSKVINGADVFIGLSVGNILTGDMVKLMDRDPVIFALANPTPEIDPGLAREAGAEIIATGRSDYANQINNVLA
FPGVLRGALDVRARDINDKMKLAAAHAIARLAEKELNKEYIIPAPFDSRVAPEVAAAVAKVAMETGVARINISPEEVKRH
TEKITEL
>Mature_407_residues
MDLYREALNMHKQKTGKIKVECKVDLKDQHDLSLAYSPGVAEPCRQIAKNKELQYEYTAKGNLVAVVSSGTAVLGLGDIG
PEAALPVMEGKAVLFKKFAGIDAFPLCVGSKDVDDIVNFVKLLEPTFAGINLEDIAAPECFEIEKRLKRETNMAIFHDDQ
HGTAIVTLAGLINALKLVNKDIASVKIVVNGAGASATAVTKLLLEAGAREIIVCDRLGALYSGRSEHMNDAKIELAEITN
PGKVKGPLSKVINGADVFIGLSVGNILTGDMVKLMDRDPVIFALANPTPEIDPGLAREAGAEIIATGRSDYANQINNVLA
FPGVLRGALDVRARDINDKMKLAAAHAIARLAEKELNKEYIIPAPFDSRVAPEVAAAVAKVAMETGVARINISPEEVKRH
TEKITEL

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=443, Percent_Identity=23.7020316027088, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI4505145, Length=413, Percent_Identity=25.90799031477, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI62420882, Length=395, Percent_Identity=27.3417721518987, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI62420880, Length=395, Percent_Identity=27.3417721518987, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI239049447, Length=395, Percent_Identity=27.3417721518987, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI270265879, Length=372, Percent_Identity=25.5376344086022, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1788806, Length=424, Percent_Identity=51.8867924528302, Blast_Score=391, Evalue=1e-110,
Organism=Escherichia coli, GI87081919, Length=444, Percent_Identity=23.6486486486486, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17537199, Length=437, Percent_Identity=25.858123569794, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI78707242, Length=403, Percent_Identity=26.7990074441687, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI78707238, Length=403, Percent_Identity=26.7990074441687, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281362672, Length=450, Percent_Identity=23.7777777777778, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI281362674, Length=450, Percent_Identity=23.7777777777778, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21356279, Length=450, Percent_Identity=23.7777777777778, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24646388, Length=399, Percent_Identity=27.3182957393484, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24646386, Length=399, Percent_Identity=27.3182957393484, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI281363505, Length=408, Percent_Identity=22.3039215686275, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI78707236, Length=408, Percent_Identity=22.3039215686275, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281363503, Length=408, Percent_Identity=22.3039215686275, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI78707232, Length=397, Percent_Identity=22.9219143576826, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 43633; Mature: 43633

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLYREALNMHKQKTGKIKVECKVDLKDQHDLSLAYSPGVAEPCRQIAKNKELQYEYTAK
CCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEECCCCHHHHHHHHCCCCEEEEEECC
GNLVAVVSSGTAVLGLGDIGPEAALPVMEGKAVLFKKFAGIDAFPLCVGSKDVDDIVNFV
CCEEEEEECCCEEEEECCCCCCCCCCHHCCCHHHHHHHCCCCCEEEECCCCCHHHHHHHH
KLLEPTFAGINLEDIAAPECFEIEKRLKRETNMAIFHDDQHGTAIVTLAGLINALKLVNK
HHHHHHHCCCCHHHHCCCHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCC
DIASVKIVVNGAGASATAVTKLLLEAGAREIIVCDRLGALYSGRSEHMNDAKIELAEITN
CCCEEEEEEECCCCHHHHHHHHHHHCCCCEEEEECHHHHHHCCCCCCCCCCEEEEEECCC
PGKVKGPLSKVINGADVFIGLSVGNILTGDMVKLMDRDPVIFALANPTPEIDPGLAREAG
CCCCCCHHHHHCCCCEEEEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHCC
AEIIATGRSDYANQINNVLAFPGVLRGALDVRARDINDKMKLAAAHAIARLAEKELNKEY
CEEEEECCHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCE
IIPAPFDSRVAPEVAAAVAKVAMETGVARINISPEEVKRHTEKITEL
EECCCCCCCCCHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHCC
>Mature Secondary Structure
MDLYREALNMHKQKTGKIKVECKVDLKDQHDLSLAYSPGVAEPCRQIAKNKELQYEYTAK
CCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEECCCCHHHHHHHHCCCCEEEEEECC
GNLVAVVSSGTAVLGLGDIGPEAALPVMEGKAVLFKKFAGIDAFPLCVGSKDVDDIVNFV
CCEEEEEECCCEEEEECCCCCCCCCCHHCCCHHHHHHHCCCCCEEEECCCCCHHHHHHHH
KLLEPTFAGINLEDIAAPECFEIEKRLKRETNMAIFHDDQHGTAIVTLAGLINALKLVNK
HHHHHHHCCCCHHHHCCCHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCC
DIASVKIVVNGAGASATAVTKLLLEAGAREIIVCDRLGALYSGRSEHMNDAKIELAEITN
CCCEEEEEEECCCCHHHHHHHHHHHCCCCEEEEECHHHHHHCCCCCCCCCCEEEEEECCC
PGKVKGPLSKVINGADVFIGLSVGNILTGDMVKLMDRDPVIFALANPTPEIDPGLAREAG
CCCCCCHHHHHCCCCEEEEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHCC
AEIIATGRSDYANQINNVLAFPGVLRGALDVRARDINDKMKLAAAHAIARLAEKELNKEY
CEEEEECCHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCE
IIPAPFDSRVAPEVAAAVAKVAMETGVARINISPEEVKRHTEKITEL
EECCCCCCCCCHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]