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The map label for this gene is pyrB

Identifier: 220931780

GI number: 220931780

Start: 1010401

End: 1011342

Strand: Direct

Name: pyrB

Synonym: Hore_09370

Alternate gene names: 220931780

Gene position: 1010401-1011342 (Clockwise)

Preceding gene: 220931779

Following gene: 220931781

Centisome position: 39.19

GC content: 44.69

Gene sequence:

>942_bases
ATGGCTTTGAAAAGAAAAGATTTTCTTGGTTTATACAATGTAGAGGCAGATGAAATAAATGAAATACTGGATACGGCCCA
GGCCATGAAGGAAATTTTTACAAGAACAGTAAAAAAAGTCCCGACCCTTAGGGGTAAAACTATTATAAATCTGTTTTTTG
AGCCGAGTACCCGGACCAAGTCATCTTTTGATATAGCTGCCAAGAGGCTCAGTGCTGATGTTATGAGTATTTCAAAATCT
TCCAGCAGTGTGGCCAAGGGAGAGACTTTACTTGATACAGCCCGGACCCTTGAAGTTATGGGAGCTGATGTTGTGGTTGT
CAGGCACAGTGCCCCGGGGGCAGCCCGGTTTCTGGCAGAAAACCTTTCAGCCAGTGTCCTCAATGCCGGGGATGGAGCTC
ATGCTCATCCAACCCAGGCTTTACTGGATATGTATACTATTAAGGAAAAGATGGGGAAAATAGAAGGTTTGAATGTTTTA
ATTATCGGGGATATAGCCCACAGCAGGGTGGCCCGGTCCAATATCTGGGGGTTAACTAAACTGGGGGCCAGGGTTCGCGT
TGTTGGACCTGGTACCCTTATCCCCAGGGATATAGAGGAGATGGGGGTTGAGGTATACCGTGACCTTGATGAAGCCCTTG
AAGGTGTGGATGTTGTAAACATTTTAAGAATACAGCTGGAAAGACAGCAAAAAGGTTTGTTCCCTTCCATCAGGGAATAC
AGGCATTTTTACGGAATGAATGAAGTAAGACTAAGGAAACTGGAAGATAGAGCATTGGTTATGCATCCTGGTCCCATGAA
CAGGGGTATAGAGATTGAAAGTATTGTTGCCGATGGGGAACAATCTGTCATCACTGAACAGGTTACTAACGGGGTTGCTA
TAAGAATGGCTTTATTATACTTACTGGCCGGGAGGGAGATAAATGATGAGGACCTTGCTTAA

Upstream 100 bases:

>100_bases
CCTGATGGTAATCCATCAGGGTTTTCTTTAAATGCTTTAACCTGAACCAGGCCACTTAACACGTTAATAAAAATGTATTA
TTAAGGAAAGGGGGACTAAA

Downstream 100 bases:

>100_bases
AAACGGTACAGTAATTGATCCGGTTAATAATATCAGGGGCCAATATGATATTTTGATAGAAGGAAAACACATCATTAATA
TAAAAAAAGAAATTAAACCA

Product: aspartate carbamoyltransferase

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MALKRKDFLGLYNVEADEINEILDTAQAMKEIFTRTVKKVPTLRGKTIINLFFEPSTRTKSSFDIAAKRLSADVMSISKS
SSSVAKGETLLDTARTLEVMGADVVVVRHSAPGAARFLAENLSASVLNAGDGAHAHPTQALLDMYTIKEKMGKIEGLNVL
IIGDIAHSRVARSNIWGLTKLGARVRVVGPGTLIPRDIEEMGVEVYRDLDEALEGVDVVNILRIQLERQQKGLFPSIREY
RHFYGMNEVRLRKLEDRALVMHPGPMNRGIEIESIVADGEQSVITEQVTNGVAIRMALLYLLAGREINDEDLA

Sequences:

>Translated_313_residues
MALKRKDFLGLYNVEADEINEILDTAQAMKEIFTRTVKKVPTLRGKTIINLFFEPSTRTKSSFDIAAKRLSADVMSISKS
SSSVAKGETLLDTARTLEVMGADVVVVRHSAPGAARFLAENLSASVLNAGDGAHAHPTQALLDMYTIKEKMGKIEGLNVL
IIGDIAHSRVARSNIWGLTKLGARVRVVGPGTLIPRDIEEMGVEVYRDLDEALEGVDVVNILRIQLERQQKGLFPSIREY
RHFYGMNEVRLRKLEDRALVMHPGPMNRGIEIESIVADGEQSVITEQVTNGVAIRMALLYLLAGREINDEDLA
>Mature_312_residues
ALKRKDFLGLYNVEADEINEILDTAQAMKEIFTRTVKKVPTLRGKTIINLFFEPSTRTKSSFDIAAKRLSADVMSISKSS
SSVAKGETLLDTARTLEVMGADVVVVRHSAPGAARFLAENLSASVLNAGDGAHAHPTQALLDMYTIKEKMGKIEGLNVLI
IGDIAHSRVARSNIWGLTKLGARVRVVGPGTLIPRDIEEMGVEVYRDLDEALEGVDVVNILRIQLERQQKGLFPSIREYR
HFYGMNEVRLRKLEDRALVMHPGPMNRGIEIESIVADGEQSVITEQVTNGVAIRMALLYLLAGREINDEDLA

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=314, Percent_Identity=34.3949044585987, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI38788445, Length=205, Percent_Identity=28.2926829268293, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI2367364, Length=317, Percent_Identity=32.4921135646688, Blast_Score=144, Evalue=6e-36,
Organism=Escherichia coli, GI1790703, Length=192, Percent_Identity=31.7708333333333, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1786469, Length=196, Percent_Identity=29.5918367346939, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI193204318, Length=331, Percent_Identity=34.1389728096677, Blast_Score=143, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6322331, Length=273, Percent_Identity=34.4322344322344, Blast_Score=144, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24642586, Length=316, Percent_Identity=34.4936708860759, Blast_Score=154, Evalue=6e-38,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_HALOH (B8CWM4)

Other databases:

- EMBL:   CP001098
- RefSeq:   YP_002508688.1
- ProteinModelPortal:   B8CWM4
- SMR:   B8CWM4
- GeneID:   7313430
- GenomeReviews:   CP001098_GR
- KEGG:   hor:Hore_09370
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   CLSK2806342
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 34551; Mature: 34420

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALKRKDFLGLYNVEADEINEILDTAQAMKEIFTRTVKKVPTLRGKTIINLFFEPSTRTK
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCC
SSFDIAAKRLSADVMSISKSSSSVAKGETLLDTARTLEVMGADVVVVRHSAPGAARFLAE
CHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHH
NLSASVLNAGDGAHAHPTQALLDMYTIKEKMGKIEGLNVLIIGDIAHSRVARSNIWGLTK
HHHHHHEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHCCCHHH
LGARVRVVGPGTLIPRDIEEMGVEVYRDLDEALEGVDVVNILRIQLERQQKGLFPSIREY
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHH
RHFYGMNEVRLRKLEDRALVMHPGPMNRGIEIESIVADGEQSVITEQVTNGVAIRMALLY
HHHCCCCHHHHHHHCCCEEEECCCCCCCCCEEEHHHCCCHHHHHHHHHHCCHHHHHHHHH
LLAGREINDEDLA
HHHCCCCCCCCCC
>Mature Secondary Structure 
ALKRKDFLGLYNVEADEINEILDTAQAMKEIFTRTVKKVPTLRGKTIINLFFEPSTRTK
CCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCC
SSFDIAAKRLSADVMSISKSSSSVAKGETLLDTARTLEVMGADVVVVRHSAPGAARFLAE
CHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHH
NLSASVLNAGDGAHAHPTQALLDMYTIKEKMGKIEGLNVLIIGDIAHSRVARSNIWGLTK
HHHHHHEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHCCCHHH
LGARVRVVGPGTLIPRDIEEMGVEVYRDLDEALEGVDVVNILRIQLERQQKGLFPSIREY
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHH
RHFYGMNEVRLRKLEDRALVMHPGPMNRGIEIESIVADGEQSVITEQVTNGVAIRMALLY
HHHCCCCHHHHHHHCCCEEEECCCCCCCCCEEEHHHCCCHHHHHHHHHHCCHHHHHHHHH
LLAGREINDEDLA
HHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA