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The map label for this gene is proS

Identifier: 220931621

GI number: 220931621

Start: 832581

End: 834287

Strand: Direct

Name: proS

Synonym: Hore_07770

Alternate gene names: 220931621

Gene position: 832581-834287 (Clockwise)

Preceding gene: 220931620

Following gene: 220931622

Centisome position: 32.29

GC content: 39.13

Gene sequence:

>1707_bases
ATGCGCATGTCAAACCTTTATATACCTACTTTAAAGGAAGTTCCTAAAGAAGCTGAGGTTGTAAGCCACCAGTTAATGCT
CAGAGCAGGGCTTATAAGGAATTTAACAGCAGGTGTTTATTCTTTTCTACCGTTGGGATATAGAGTAATTAAAAAGATTG
AAGGTATAATCAGGAAAGTTATGGATGAGAGTGGTGCCCAGGAAGTATTGATGCCGGTGATTCATACATCAGACCTCTGG
AAAGAATCTGGCAGGTGGGAAAAATTTGGACCCCTGATGATTAAATTTGAAGACAGGAAAGGTAGGGAATATTGCCTCGG
CCCCACCCATGAAGAAGTAATTACAGACCTTGTCAGGGATGAAATTAGATCATATAAAGACCTGCCTTTAAACCTTTATC
AAATTCAAACAAAGGTAAGGGATGAAATAAGGCCAAGGTTTGGTTTAATGAGAAGTCGGGAATTTATAATGAAGGATGCC
TATAGTTTTGATAGAGATTATGAGGGTCTGGATAAGAGTTATCAGGTAATGTATGGAGCCTATACTGAAGTTTTTAATAG
ATGTGGTCTAGAGGTCAGGGCTGTAGAAGCCGACACTGGTGCCATGGGGGGTAAGGATTCCCATGAATTTATGGTGCTGG
CTGAATCAGGTGAAGATGACATTGCTTTCTGTAACAGATGTGATTATGCTGCCAATGTTGAGAGAGCGGAAGCCCGATAT
GTAAAAAGCGAGAAAAATGAGGAAATTAAACCCCTGGAAAAGGTAGAAACACCCGGGAAGAAAAAGATAGATGAGCTGGT
TGAATTTTTAAAAATGCCTTCCGAAAAGATGATCAAAGCTGTAGCTTATTTAGCTGATGGAGAGCCAGTCCTTGCCTTGG
TCAGGGGTGATGATGAATTAAATGAGGTTAAATTAATTAATTACCTGGATGTTATAGAACTCACCCCTGTTGTTGATGAA
GATTTTCCACAGTATTATAATAGTTATGCCGGTTATGTTGGGCCCATTGGTCTAAAAAATGTCAGGATTATTGCTGATAG
GAAAGTTAAAGATATTGTCAATGGAATATGCGGCGCCAATGAAGAAAATTATCACTTTATCAATGTTAACCCTGAACGTG
ATTTCAGTGTAGAAAAATACCTCGATATAAGGAAGGTTAAAGAAGGTGACCAATGTCCCCATTGTGAGGGTACAATTGAG
ATAAAATCGGGAATAGAGGTAGGACATATTTTTAAACTGGGAACCAAGTATAGTGAAAGCATGGGGGCTACTTATCTTGA
TGAAAATGGAAAAGAGCAGCCAATTGTTATGGGGAGTTACGGGATAGGTGTTACCAGATTAGTTGCTGCTGCAATAGAGC
AAAACCATGATGAACATGGAATTATCTGGCCTAAAGCTATTGCCCCCTATCAGGTAATTATTTTACCCCTTGGAAAGGGA
GATGAGGTAAATAAAAGAGCCGAAGAGCTTTATCTAGAACTTAAAGATAATAATATTGAAGTACTTCTTGATGACCGTAA
TGAAAGAGCCGGGGTAAAATTTAATGACGCTGACCTGATCGGTATTCCCCTGAGATTAACTATTGGTTCCCGGTCTCTGG
ATAAAGGAGTAATTGAAGCACGGGTCAGGGGAACAGGCCAGGATTATGAAATATCTGTTGAAGATGCTATAAAGGATATA
AAGAAATTACTAGAAAATGTTAAATAG

Upstream 100 bases:

>100_bases
TTAAAAAGGGTAAATTAATAAAAAAAGTTAAGGAAGATGAACTATTAGATTGTTTGCTTGAAGAAATAAATAAAATAAGA
TAATATAAGGAGTGATCTAT

Downstream 100 bases:

>100_bases
ATAGATACTTTTTAGATAGATACTTTATAGTATAGATAATAACTTCAGTATAACTTTTTATAAATTATTGTATATAATAT
ATAAAGGAGGACCAGAATGA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 568; Mature: 568

Protein sequence:

>568_residues
MRMSNLYIPTLKEVPKEAEVVSHQLMLRAGLIRNLTAGVYSFLPLGYRVIKKIEGIIRKVMDESGAQEVLMPVIHTSDLW
KESGRWEKFGPLMIKFEDRKGREYCLGPTHEEVITDLVRDEIRSYKDLPLNLYQIQTKVRDEIRPRFGLMRSREFIMKDA
YSFDRDYEGLDKSYQVMYGAYTEVFNRCGLEVRAVEADTGAMGGKDSHEFMVLAESGEDDIAFCNRCDYAANVERAEARY
VKSEKNEEIKPLEKVETPGKKKIDELVEFLKMPSEKMIKAVAYLADGEPVLALVRGDDELNEVKLINYLDVIELTPVVDE
DFPQYYNSYAGYVGPIGLKNVRIIADRKVKDIVNGICGANEENYHFINVNPERDFSVEKYLDIRKVKEGDQCPHCEGTIE
IKSGIEVGHIFKLGTKYSESMGATYLDENGKEQPIVMGSYGIGVTRLVAAAIEQNHDEHGIIWPKAIAPYQVIILPLGKG
DEVNKRAEELYLELKDNNIEVLLDDRNERAGVKFNDADLIGIPLRLTIGSRSLDKGVIEARVRGTGQDYEISVEDAIKDI
KKLLENVK

Sequences:

>Translated_568_residues
MRMSNLYIPTLKEVPKEAEVVSHQLMLRAGLIRNLTAGVYSFLPLGYRVIKKIEGIIRKVMDESGAQEVLMPVIHTSDLW
KESGRWEKFGPLMIKFEDRKGREYCLGPTHEEVITDLVRDEIRSYKDLPLNLYQIQTKVRDEIRPRFGLMRSREFIMKDA
YSFDRDYEGLDKSYQVMYGAYTEVFNRCGLEVRAVEADTGAMGGKDSHEFMVLAESGEDDIAFCNRCDYAANVERAEARY
VKSEKNEEIKPLEKVETPGKKKIDELVEFLKMPSEKMIKAVAYLADGEPVLALVRGDDELNEVKLINYLDVIELTPVVDE
DFPQYYNSYAGYVGPIGLKNVRIIADRKVKDIVNGICGANEENYHFINVNPERDFSVEKYLDIRKVKEGDQCPHCEGTIE
IKSGIEVGHIFKLGTKYSESMGATYLDENGKEQPIVMGSYGIGVTRLVAAAIEQNHDEHGIIWPKAIAPYQVIILPLGKG
DEVNKRAEELYLELKDNNIEVLLDDRNERAGVKFNDADLIGIPLRLTIGSRSLDKGVIEARVRGTGQDYEISVEDAIKDI
KKLLENVK
>Mature_568_residues
MRMSNLYIPTLKEVPKEAEVVSHQLMLRAGLIRNLTAGVYSFLPLGYRVIKKIEGIIRKVMDESGAQEVLMPVIHTSDLW
KESGRWEKFGPLMIKFEDRKGREYCLGPTHEEVITDLVRDEIRSYKDLPLNLYQIQTKVRDEIRPRFGLMRSREFIMKDA
YSFDRDYEGLDKSYQVMYGAYTEVFNRCGLEVRAVEADTGAMGGKDSHEFMVLAESGEDDIAFCNRCDYAANVERAEARY
VKSEKNEEIKPLEKVETPGKKKIDELVEFLKMPSEKMIKAVAYLADGEPVLALVRGDDELNEVKLINYLDVIELTPVVDE
DFPQYYNSYAGYVGPIGLKNVRIIADRKVKDIVNGICGANEENYHFINVNPERDFSVEKYLDIRKVKEGDQCPHCEGTIE
IKSGIEVGHIFKLGTKYSESMGATYLDENGKEQPIVMGSYGIGVTRLVAAAIEQNHDEHGIIWPKAIAPYQVIILPLGKG
DEVNKRAEELYLELKDNNIEVLLDDRNERAGVKFNDADLIGIPLRLTIGSRSLDKGVIEARVRGTGQDYEISVEDAIKDI
KKLLENVK

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=214, Percent_Identity=45.3271028037383, Blast_Score=209, Evalue=5e-54,
Organism=Escherichia coli, GI1786392, Length=569, Percent_Identity=51.3181019332162, Blast_Score=572, Evalue=1e-164,
Organism=Caenorhabditis elegans, GI115532348, Length=216, Percent_Identity=40.7407407407407, Blast_Score=173, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI193203271, Length=91, Percent_Identity=39.5604395604396, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71991342, Length=172, Percent_Identity=29.0697674418605, Blast_Score=72, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6320931, Length=542, Percent_Identity=31.549815498155, Blast_Score=241, Evalue=3e-64,
Organism=Drosophila melanogaster, GI24656200, Length=245, Percent_Identity=35.1020408163265, Blast_Score=169, Evalue=5e-42,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_HALOH (B8CW65)

Other databases:

- EMBL:   CP001098
- RefSeq:   YP_002508529.1
- ProteinModelPortal:   B8CW65
- SMR:   B8CW65
- GeneID:   7314764
- GenomeReviews:   CP001098_GR
- KEGG:   hor:Hore_07770
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   CLSK2806296
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 64502; Mature: 64502

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMSNLYIPTLKEVPKEAEVVSHQLMLRAGLIRNLTAGVYSFLPLGYRVIKKIEGIIRKV
CCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MDESGAQEVLMPVIHTSDLWKESGRWEKFGPLMIKFEDRKGREYCLGPTHEEVITDLVRD
HCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHH
EIRSYKDLPLNLYQIQTKVRDEIRPRFGLMRSREFIMKDAYSFDRDYEGLDKSYQVMYGA
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
YTEVFNRCGLEVRAVEADTGAMGGKDSHEFMVLAESGEDDIAFCNRCDYAANVERAEARY
HHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCCHHHHCCCCCCCCCHHHHHHH
VKSEKNEEIKPLEKVETPGKKKIDELVEFLKMPSEKMIKAVAYLADGEPVLALVRGDDEL
HHCCCCCCCCCHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEECCCCC
NEVKLINYLDVIELTPVVDEDFPQYYNSYAGYVGPIGLKNVRIIADRKVKDIVNGICGAN
CCEEHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCC
EENYHFINVNPERDFSVEKYLDIRKVKEGDQCPHCEGTIEIKSGIEVGHIFKLGTKYSES
CCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEECCCCCEEHEEECCCHHHHH
MGATYLDENGKEQPIVMGSYGIGVTRLVAAAIEQNHDEHGIIWPKAIAPYQVIILPLGKG
CCCEEECCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCEECCCCCCCEEEEEEECCCC
DEVNKRAEELYLELKDNNIEVLLDDRNERAGVKFNDADLIGIPLRLTIGSRSLDKGVIEA
CCHHHHHHHEEEEEECCCEEEEEECCCCCCCCEECCCCEEEEEEEEEECCCCHHHHHHHH
RVRGTGQDYEISVEDAIKDIKKLLENVK
HCCCCCCCEEEEHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRMSNLYIPTLKEVPKEAEVVSHQLMLRAGLIRNLTAGVYSFLPLGYRVIKKIEGIIRKV
CCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MDESGAQEVLMPVIHTSDLWKESGRWEKFGPLMIKFEDRKGREYCLGPTHEEVITDLVRD
HCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHH
EIRSYKDLPLNLYQIQTKVRDEIRPRFGLMRSREFIMKDAYSFDRDYEGLDKSYQVMYGA
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
YTEVFNRCGLEVRAVEADTGAMGGKDSHEFMVLAESGEDDIAFCNRCDYAANVERAEARY
HHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCCHHHHCCCCCCCCCHHHHHHH
VKSEKNEEIKPLEKVETPGKKKIDELVEFLKMPSEKMIKAVAYLADGEPVLALVRGDDEL
HHCCCCCCCCCHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEECCCCC
NEVKLINYLDVIELTPVVDEDFPQYYNSYAGYVGPIGLKNVRIIADRKVKDIVNGICGAN
CCEEHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCC
EENYHFINVNPERDFSVEKYLDIRKVKEGDQCPHCEGTIEIKSGIEVGHIFKLGTKYSES
CCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEECCCCCEEHEEECCCHHHHH
MGATYLDENGKEQPIVMGSYGIGVTRLVAAAIEQNHDEHGIIWPKAIAPYQVIILPLGKG
CCCEEECCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCEECCCCCCCEEEEEEECCCC
DEVNKRAEELYLELKDNNIEVLLDDRNERAGVKFNDADLIGIPLRLTIGSRSLDKGVIEA
CCHHHHHHHEEEEEECCCEEEEEECCCCCCCCEECCCCEEEEEEEEEECCCCHHHHHHHH
RVRGTGQDYEISVEDAIKDIKKLLENVK
HCCCCCCCEEEEHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA