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The map label for this gene is ispG [H]

Identifier: 220931620

GI number: 220931620

Start: 831514

End: 832563

Strand: Direct

Name: ispG [H]

Synonym: Hore_07760

Alternate gene names: 220931620

Gene position: 831514-832563 (Clockwise)

Preceding gene: 220931619

Following gene: 220931621

Centisome position: 32.25

GC content: 39.43

Gene sequence:

>1050_bases
ATGAGACGAAAAAGTCGAAAAATATATTATGGCAATATTCCGGTAGGTGGGGATTCCCCAATTACCGTGCAATCTATGAC
AAATACAAAAACCGCTGATGTCAACGCTACAGTTAAACAGATAAAAAGGCTTGAAAAAGCAGGATGTGAAATAATAAGGG
TTGCCGTTCCCGATATGGAATCTGCCCGGAAGCTTTCATTGATAAAAAAGAGGATTAGTATACCCCTTATAGCTGATATT
CATTTTGATTATAGACTGGCCCTGGAAGCTATACATCAGGGGGTTGATGGTATAAGAATAAACCCCGGTAATATTGGGAG
TGAGGAGAAGGTAGTTAAAGTCATTGAGGCTGCTAAAACGGCCGGTATCCCCCTCAGAATAGGAGTAAATTCTGGGTCTA
TTGAAAAATCATTAATTGAAAAATACGGGGGGCCCACAGCAGAAGCAATGGTAGAAAGTGCCTTAAAACATGTTTACATC
CTTGAAAAACATAGTTTTTATGATATTATAATTTCCTTAAAATCTACTGATATCTGGACTACTGTTGAGTCCTATCATTT
AATGGCTAAAAAGGTTGACTATCCCTTCCATATAGGTATTACTGAAGCTGGAACACCCTGGTCGGGTACAATCAAATCAT
CGATTGGCATTGGAGCCCTTTTATTAGAGGGACTGGGTGATACCCTCAGGGTTTCCCTGACCGGAGACCCGGTGGAAGAA
GTCAGGGTCGGCTGGCAGATTTTAAAATTATTAAACTTAAGAAAGAGTGGTCCTGTTGTTATTTCCTGTCCTACCTGTGG
CCGGACCGAGATTGATTTAATAAAACTTGCTGAAGAGGTAGAAAAAAAGGTAAGTTCCCTGAAAAAACCCCTGACGATTG
CAGTTATGGGTTGCGCCGTTAACGGACCCGGTGAAGCTAAAGATGCCGATATAGGTATTGCCGGTGGTAAAGGGGAAGGA
ATTATATTTAAAAAGGGTAAATTAATAAAAAAAGTTAAGGAAGATGAACTATTAGATTGTTTGCTTGAAGAAATAAATAA
AATAAGATAA

Upstream 100 bases:

>100_bases
AAGCCCTCGAAAAGGTAAAAAACCTGGACAGGCTTGTTCAGGATGAAATAAAAGATTGTGACGATAACTGTGAAAGTTGT
CATAGAAGATAGGTGGTTTG

Downstream 100 bases:

>100_bases
TATAAGGAGTGATCTATATGCGCATGTCAAACCTTTATATACCTACTTTAAAGGAAGTTCCTAAAGAAGCTGAGGTTGTA
AGCCACCAGTTAATGCTCAG

Product: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MRRKSRKIYYGNIPVGGDSPITVQSMTNTKTADVNATVKQIKRLEKAGCEIIRVAVPDMESARKLSLIKKRISIPLIADI
HFDYRLALEAIHQGVDGIRINPGNIGSEEKVVKVIEAAKTAGIPLRIGVNSGSIEKSLIEKYGGPTAEAMVESALKHVYI
LEKHSFYDIIISLKSTDIWTTVESYHLMAKKVDYPFHIGITEAGTPWSGTIKSSIGIGALLLEGLGDTLRVSLTGDPVEE
VRVGWQILKLLNLRKSGPVVISCPTCGRTEIDLIKLAEEVEKKVSSLKKPLTIAVMGCAVNGPGEAKDADIGIAGGKGEG
IIFKKGKLIKKVKEDELLDCLLEEINKIR

Sequences:

>Translated_349_residues
MRRKSRKIYYGNIPVGGDSPITVQSMTNTKTADVNATVKQIKRLEKAGCEIIRVAVPDMESARKLSLIKKRISIPLIADI
HFDYRLALEAIHQGVDGIRINPGNIGSEEKVVKVIEAAKTAGIPLRIGVNSGSIEKSLIEKYGGPTAEAMVESALKHVYI
LEKHSFYDIIISLKSTDIWTTVESYHLMAKKVDYPFHIGITEAGTPWSGTIKSSIGIGALLLEGLGDTLRVSLTGDPVEE
VRVGWQILKLLNLRKSGPVVISCPTCGRTEIDLIKLAEEVEKKVSSLKKPLTIAVMGCAVNGPGEAKDADIGIAGGKGEG
IIFKKGKLIKKVKEDELLDCLLEEINKIR
>Mature_349_residues
MRRKSRKIYYGNIPVGGDSPITVQSMTNTKTADVNATVKQIKRLEKAGCEIIRVAVPDMESARKLSLIKKRISIPLIADI
HFDYRLALEAIHQGVDGIRINPGNIGSEEKVVKVIEAAKTAGIPLRIGVNSGSIEKSLIEKYGGPTAEAMVESALKHVYI
LEKHSFYDIIISLKSTDIWTTVESYHLMAKKVDYPFHIGITEAGTPWSGTIKSSIGIGALLLEGLGDTLRVSLTGDPVEE
VRVGWQILKLLNLRKSGPVVISCPTCGRTEIDLIKLAEEVEKKVSSLKKPLTIAVMGCAVNGPGEAKDADIGIAGGKGEG
IIFKKGKLIKKVKEDELLDCLLEEINKIR

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=340, Percent_Identity=50.2941176470588, Blast_Score=377, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 37959; Mature: 37959

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRKSRKIYYGNIPVGGDSPITVQSMTNTKTADVNATVKQIKRLEKAGCEIIRVAVPDME
CCCCCCEEEECCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCHH
SARKLSLIKKRISIPLIADIHFDYRLALEAIHQGVDGIRINPGNIGSEEKVVKVIEAAKT
HHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHH
AGIPLRIGVNSGSIEKSLIEKYGGPTAEAMVESALKHVYILEKHSFYDIIISLKSTDIWT
CCCEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHEEEEECCCEEEEEEEEECCCEEE
TVESYHLMAKKVDYPFHIGITEAGTPWSGTIKSSIGIGALLLEGLGDTLRVSLTGDPVEE
HHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHCCCCHHHHHHHCCCCEEEEEECCCCHHH
VRVGWQILKLLNLRKSGPVVISCPTCGRTEIDLIKLAEEVEKKVSSLKKPLTIAVMGCAV
HHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEE
NGPGEAKDADIGIAGGKGEGIIFKKGKLIKKVKEDELLDCLLEEINKIR
CCCCCCCCCCEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRRKSRKIYYGNIPVGGDSPITVQSMTNTKTADVNATVKQIKRLEKAGCEIIRVAVPDME
CCCCCCEEEECCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCHH
SARKLSLIKKRISIPLIADIHFDYRLALEAIHQGVDGIRINPGNIGSEEKVVKVIEAAKT
HHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHH
AGIPLRIGVNSGSIEKSLIEKYGGPTAEAMVESALKHVYILEKHSFYDIIISLKSTDIWT
CCCEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHEEEEECCCEEEEEEEEECCCEEE
TVESYHLMAKKVDYPFHIGITEAGTPWSGTIKSSIGIGALLLEGLGDTLRVSLTGDPVEE
HHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHCCCCHHHHHHHCCCCEEEEEECCCCHHH
VRVGWQILKLLNLRKSGPVVISCPTCGRTEIDLIKLAEEVEKKVSSLKKPLTIAVMGCAV
HHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEE
NGPGEAKDADIGIAGGKGEGIIFKKGKLIKKVKEDELLDCLLEEINKIR
CCCCCCCCCCEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792842 [H]