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The map label for this gene is spo0A [H]

Identifier: 220931503

GI number: 220931503

Start: 715163

End: 715951

Strand: Direct

Name: spo0A [H]

Synonym: Hore_06590

Alternate gene names: 220931503

Gene position: 715163-715951 (Clockwise)

Preceding gene: 220931502

Following gene: 220931504

Centisome position: 27.74

GC content: 39.54

Gene sequence:

>789_bases
TTGGAAGATAATGTTAGAATTCTGCTAGTTGATGATAACAAAGAATTCTGTCAGCTACTCGAAGAATACCTTGAAGAGCA
GGAAAAATTTGAAGTGGTTGGTACAGCTCATAATGGAGTTGATGCTTTAGAACTCCTGAAGAGCAAAGAACCAGATGTTC
TTGTGCTTGATTTAATCATGCCACATCTGGATGGGATTGGAGTAATGGAAGAAATGAACAGGTCACGGATAGGTGGAGAT
GTTAAGACCATTATCTTAACCACTTTTGGTCAGGAAGAAGTAACCCAGCGGGTAGTAGAATTAGGGGCAAACTATTACAT
AATGAAGCCCTTTGACCTTGATAAGCTAGTGGAACGAATTAATCAACTTATGACCCCTCCTTCTGGCTCAAGTGGAGGTT
ATGCCATATCCCAGAATAATTCCAGGAATAGTGGGGTCGATTTAAATGTAAGGATTACTGAAATATTACATCAGCTCGGG
GTTCCAGCCCATATAAAAGGATATCTTTATTTAAGAGAGGCTATTGAACTTGTTATTAAAAATGTAGAATTGCTGGGAGC
GGTTACAAAAGAGTTATATCCAAGTGTTGCTGAGAGATATAATACGACTCCCAGTCGAGTGGAACGTGCCATACGTCATG
CTATTGAAGTATGCTGGGACAGGGGTAATGTTTCTGTTTTAAATAAGTATTTCGGGAGTACAGTTTCTTCAAATAGTGGT
AAGCCAACCAACTCCCAGTTTATAGCCAAAATCGCTGATAAACTCCGCCTTGAAATAATGGCCAGTTAA

Upstream 100 bases:

>100_bases
TTAAAAAATATTTATTTTGAAAAAAAGGTATTTGGAAATATATGTAGAATAGATTAATAGAAATTAATTAGGAGGAGAAA
TTAATAGGAGGGTGATTGTT

Downstream 100 bases:

>100_bases
CTATTATATCTTCCGGGATATAACTTCAAAATATACCAGTATGTTAATATTTATATCTCCATATAATAAGAAAAAGCCCC
GGGGGAAAACATATGATAAA

Product: response regulator receiver protein

Products: protein L-glutamate+ methanol; methanol; Tapglu; methanol; Targlu; Trgglu; Tsrglu; NH3; protein-L-glutamate [C]

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MEDNVRILLVDDNKEFCQLLEEYLEEQEKFEVVGTAHNGVDALELLKSKEPDVLVLDLIMPHLDGIGVMEEMNRSRIGGD
VKTIILTTFGQEEVTQRVVELGANYYIMKPFDLDKLVERINQLMTPPSGSSGGYAISQNNSRNSGVDLNVRITEILHQLG
VPAHIKGYLYLREAIELVIKNVELLGAVTKELYPSVAERYNTTPSRVERAIRHAIEVCWDRGNVSVLNKYFGSTVSSNSG
KPTNSQFIAKIADKLRLEIMAS

Sequences:

>Translated_262_residues
MEDNVRILLVDDNKEFCQLLEEYLEEQEKFEVVGTAHNGVDALELLKSKEPDVLVLDLIMPHLDGIGVMEEMNRSRIGGD
VKTIILTTFGQEEVTQRVVELGANYYIMKPFDLDKLVERINQLMTPPSGSSGGYAISQNNSRNSGVDLNVRITEILHQLG
VPAHIKGYLYLREAIELVIKNVELLGAVTKELYPSVAERYNTTPSRVERAIRHAIEVCWDRGNVSVLNKYFGSTVSSNSG
KPTNSQFIAKIADKLRLEIMAS
>Mature_262_residues
MEDNVRILLVDDNKEFCQLLEEYLEEQEKFEVVGTAHNGVDALELLKSKEPDVLVLDLIMPHLDGIGVMEEMNRSRIGGD
VKTIILTTFGQEEVTQRVVELGANYYIMKPFDLDKLVERINQLMTPPSGSSGGYAISQNNSRNSGVDLNVRITEILHQLG
VPAHIKGYLYLREAIELVIKNVELLGAVTKELYPSVAERYNTTPSRVERAIRHAIEVCWDRGNVSVLNKYFGSTVSSNSG
KPTNSQFIAKIADKLRLEIMAS

Specific function: May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with spo0H (a sigma factor) to control the ex

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788192, Length=107, Percent_Identity=38.3177570093458, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788550, Length=123, Percent_Identity=36.5853658536585, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789809, Length=125, Percent_Identity=37.6, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI1786599, Length=119, Percent_Identity=33.6134453781513, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR014879
- InterPro:   IPR012052
- InterPro:   IPR011991
- ProDom:   PD010908 [H]

Pfam domain/function: PF00072 Response_reg; PF08769 Spo0A_C [H]

EC number: 3.1.1.61 [C]

Molecular weight: Translated: 29351; Mature: 29351

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDNVRILLVDDNKEFCQLLEEYLEEQEKFEVVGTAHNGVDALELLKSKEPDVLVLDLIM
CCCCEEEEEEECCHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHCCCCCEEEHHHHH
PHLDGIGVMEEMNRSRIGGDVKTIILTTFGQEEVTQRVVELGANYYIMKPFDLDKLVERI
HCCCCCHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHHHHCCCEEEECCCCHHHHHHHH
NQLMTPPSGSSGGYAISQNNSRNSGVDLNVRITEILHQLGVPAHIKGYLYLREAIELVIK
HHHCCCCCCCCCCEEEECCCCCCCCCEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
NVELLGAVTKELYPSVAERYNTTPSRVERAIRHAIEVCWDRGNVSVLNKYFGSTVSSNSG
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCC
KPTNSQFIAKIADKLRLEIMAS
CCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEDNVRILLVDDNKEFCQLLEEYLEEQEKFEVVGTAHNGVDALELLKSKEPDVLVLDLIM
CCCCEEEEEEECCHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHCCCCCEEEHHHHH
PHLDGIGVMEEMNRSRIGGDVKTIILTTFGQEEVTQRVVELGANYYIMKPFDLDKLVERI
HCCCCCHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHHHHCCCEEEECCCCHHHHHHHH
NQLMTPPSGSSGGYAISQNNSRNSGVDLNVRITEILHQLGVPAHIKGYLYLREAIELVIK
HHHCCCCCCCCCCEEEECCCCCCCCCEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
NVELLGAVTKELYPSVAERYNTTPSRVERAIRHAIEVCWDRGNVSVLNKYFGSTVSSNSG
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCC
KPTNSQFIAKIADKLRLEIMAS
CCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein L-glutamate Omethyl ester; Tapglu-Me; H2O; Targlu-Me; Trgglu-Me; Tsrglu-Me; Tsrgln; Trggln; Targln; Tapgln; protein-L-glutamine [C]

Specific reaction: Protein L-glutamate Omethyl ester + H2O = protein L-glutamate+ methanol H2O + Tapglu-Me = methanol + Tapglu H2O + Targlu-Me = methanol + Targlu H2O + Trgglu-Me = methanol + Trgglu H2O + Tsrglu-Me = methanol + Tsrglu H2O + Tsrgln = NH3 + Tsrglu H2O + Trggl

General reaction: Hydrolysis of carboxylic ester [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7885226 [H]