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The map label for this gene is yfiN [C]

Identifier: 220931367

GI number: 220931367

Start: 569891

End: 571396

Strand: Reverse

Name: yfiN [C]

Synonym: Hore_05220

Alternate gene names: 220931367

Gene position: 571396-569891 (Counterclockwise)

Preceding gene: 220931368

Following gene: 220931366

Centisome position: 22.16

GC content: 30.74

Gene sequence:

>1506_bases
ATGAATAATGTTATAGAAAAACATAAATATTATATTATAATAGCTGTTTTTTTAATCATCATCTTTTTATCTTATAACAG
CTATCAGAATACCACAAACATTATTAAATCCAAATATATAACCCATCAAACCCTGATTGAAAAAAGTATTATTAATTCCA
TAAATAATACCAACTACGCCTACCAGATAGCAGAAAAACTACTTAATGATAAAATGGAGAAATTATCACGTCAATTAATA
TCTAAATACAGAGATAATCCAGATGTGCTGACCTGGGATTTAGAAAAAATCAAACAAACACATAAATATGTTAATATTTA
TATTATTGATAATAACTTAAAAATAATTCATACTACCTACAAAAAAGATTTAGGGCTTGATTTCAATAAATATCCTTTAT
TCAGTAAGGTCTTAAGAAAAAGACTTGAAGGAGATAATTTCATTGTTGACCGGATGGATATATCAATTAAATCTGGCCAG
CTTAAGAAATACAGTTATATGCCCACTCCAGATCATAAATATCTTATCGAATTAAGCATCAACCTCAAGGAGATTTTCCC
CCAGATAGATAGTATTAACCTTATTTCACTGACAGAACGTTTAAAATCAGAGTATCCCACTATTGAAGAAATAAATTTTT
ATAGAATTACTCAACAGGGAAATATAGTTACAGAATCAATAACACTCTCTGAATCACTTCTACATACCGATATTCCTGAC
AAAATTAAGTCACTTGTAAAAAAGTGTGCCAGTACCAGTCAGGTAGTTTCCAGCAAAATAAAATCAAACTCACAAAATAG
ATATTATTCAGTCAGGTTTATCCCCTGTCTTACCCAGAATCCAGAGGATCCCTACTGGTGGGATTCTTCCGTAATCGGAA
TTAAATATAATAATGAGTTTATGTTAGAAGAATTAAATCATTATAAACATGTATTTATTATAAACATGACGATAATAACC
ATTGTATTTGTTTTATTTGTTTTAGTAATAGAATATCTCCTGAAAGAAACAAAATATATGGCTTATCATGATCACCTTAC
CAAACTTCCCAACCGGGCTTTATTTGAAAGATACTTTAATAAACTTAAAAATAATACAATAAACCTTAACAAGATGGCAG
TCCTTTTCCTTGATCTCGATGACTTTAAAAATGTCAACGATACCCTGGGACATGCTATCGGTGATAAACTGCTTATTCAG
GTAGCCAAACGATTAAAAAAGAACCTTAAACCATCAGATACCTTATCAAGACTGGGTGGGGATGAATTCACTTTACTCCT
GACCAGTATTAGAACAAGGGAAGATGTGATTAAATTTGCCTCAGATTTAAGTGAAGACTTCAAAAAACCATTTATTCTAG
ATGGTAATAAACTTACAATCCACCCCAGTATAGGTATCAGCATCTACCCTGATAATGGTAAAAATCTGGACTCTCTTTTA
AAGCAGGCCGATAACGCCATGTATCAGGCTAAAAAACAAAAATTAGATTTTATCCTCTGGAGATAA

Upstream 100 bases:

>100_bases
TTTTTGTATAATTTTAAAACAAATAAATAAAGGAAAGTATACAATCAATCTAGAACTATATTATAAAGTGTCGGACTTTG
TTGAGAAATGAGGGTTTATG

Downstream 100 bases:

>100_bases
TAATGTAATACTAATTATTTAATGGCAGGATATCCTGCCATTTTTTATACTTAAAAAACTTAAAGTGACAAAAATCAACT
TTACTAAATAATATATACTA

Product: diguanylate cyclase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MNNVIEKHKYYIIIAVFLIIIFLSYNSYQNTTNIIKSKYITHQTLIEKSIINSINNTNYAYQIAEKLLNDKMEKLSRQLI
SKYRDNPDVLTWDLEKIKQTHKYVNIYIIDNNLKIIHTTYKKDLGLDFNKYPLFSKVLRKRLEGDNFIVDRMDISIKSGQ
LKKYSYMPTPDHKYLIELSINLKEIFPQIDSINLISLTERLKSEYPTIEEINFYRITQQGNIVTESITLSESLLHTDIPD
KIKSLVKKCASTSQVVSSKIKSNSQNRYYSVRFIPCLTQNPEDPYWWDSSVIGIKYNNEFMLEELNHYKHVFIINMTIIT
IVFVLFVLVIEYLLKETKYMAYHDHLTKLPNRALFERYFNKLKNNTINLNKMAVLFLDLDDFKNVNDTLGHAIGDKLLIQ
VAKRLKKNLKPSDTLSRLGGDEFTLLLTSIRTREDVIKFASDLSEDFKKPFILDGNKLTIHPSIGISIYPDNGKNLDSLL
KQADNAMYQAKKQKLDFILWR

Sequences:

>Translated_501_residues
MNNVIEKHKYYIIIAVFLIIIFLSYNSYQNTTNIIKSKYITHQTLIEKSIINSINNTNYAYQIAEKLLNDKMEKLSRQLI
SKYRDNPDVLTWDLEKIKQTHKYVNIYIIDNNLKIIHTTYKKDLGLDFNKYPLFSKVLRKRLEGDNFIVDRMDISIKSGQ
LKKYSYMPTPDHKYLIELSINLKEIFPQIDSINLISLTERLKSEYPTIEEINFYRITQQGNIVTESITLSESLLHTDIPD
KIKSLVKKCASTSQVVSSKIKSNSQNRYYSVRFIPCLTQNPEDPYWWDSSVIGIKYNNEFMLEELNHYKHVFIINMTIIT
IVFVLFVLVIEYLLKETKYMAYHDHLTKLPNRALFERYFNKLKNNTINLNKMAVLFLDLDDFKNVNDTLGHAIGDKLLIQ
VAKRLKKNLKPSDTLSRLGGDEFTLLLTSIRTREDVIKFASDLSEDFKKPFILDGNKLTIHPSIGISIYPDNGKNLDSLL
KQADNAMYQAKKQKLDFILWR
>Mature_501_residues
MNNVIEKHKYYIIIAVFLIIIFLSYNSYQNTTNIIKSKYITHQTLIEKSIINSINNTNYAYQIAEKLLNDKMEKLSRQLI
SKYRDNPDVLTWDLEKIKQTHKYVNIYIIDNNLKIIHTTYKKDLGLDFNKYPLFSKVLRKRLEGDNFIVDRMDISIKSGQ
LKKYSYMPTPDHKYLIELSINLKEIFPQIDSINLISLTERLKSEYPTIEEINFYRITQQGNIVTESITLSESLLHTDIPD
KIKSLVKKCASTSQVVSSKIKSNSQNRYYSVRFIPCLTQNPEDPYWWDSSVIGIKYNNEFMLEELNHYKHVFIINMTIIT
IVFVLFVLVIEYLLKETKYMAYHDHLTKLPNRALFERYFNKLKNNTINLNKMAVLFLDLDDFKNVNDTLGHAIGDKLLIQ
VAKRLKKNLKPSDTLSRLGGDEFTLLLTSIRTREDVIKFASDLSEDFKKPFILDGNKLTIHPSIGISIYPDNGKNLDSLL
KQADNAMYQAKKQKLDFILWR

Specific function: Unknown

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 MHYT domain [H]

Homologues:

Organism=Escherichia coli, GI1788956, Length=157, Percent_Identity=41.4012738853503, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI1788381, Length=161, Percent_Identity=39.1304347826087, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787262, Length=183, Percent_Identity=36.0655737704918, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI1787541, Length=155, Percent_Identity=35.4838709677419, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI87082007, Length=237, Percent_Identity=26.1603375527426, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI87081977, Length=158, Percent_Identity=37.9746835443038, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786584, Length=159, Percent_Identity=28.9308176100629, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI145693134, Length=189, Percent_Identity=30.1587301587302, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1787802, Length=213, Percent_Identity=26.7605633802817, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI87081881, Length=169, Percent_Identity=30.7692307692308, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1788085, Length=170, Percent_Identity=27.6470588235294, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081921, Length=144, Percent_Identity=33.3333333333333, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1787816, Length=151, Percent_Identity=31.1258278145695, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1787056, Length=163, Percent_Identity=29.4478527607362, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR005330 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF03707 MHYT [H]

EC number: NA

Molecular weight: Translated: 58711; Mature: 58711

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNVIEKHKYYIIIAVFLIIIFLSYNSYQNTTNIIKSKYITHQTLIEKSIINSINNTNYA
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
YQIAEKLLNDKMEKLSRQLISKYRDNPDVLTWDLEKIKQTHKYVNIYIIDNNLKIIHTTY
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHCEEEEEEEECCEEEEEEEH
KKDLGLDFNKYPLFSKVLRKRLEGDNFIVDRMDISIKSGQLKKYSYMPTPDHKYLIELSI
HHHCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEEEEECCCCEEECCCCCCCCCEEEEEEC
NLKEIFPQIDSINLISLTERLKSEYPTIEEINFYRITQQGNIVTESITLSESLLHTDIPD
CHHHHCCCCCCCHHHHHHHHHHHHCCCHHCCEEEEEECCCCEEEEEEEHHHHHHHCCCHH
KIKSLVKKCASTSQVVSSKIKSNSQNRYYSVRFIPCLTQNPEDPYWWDSSVIGIKYNNEF
HHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCCCCEEEEEEECCHH
MLEELNHYKHVFIINMTIITIVFVLFVLVIEYLLKETKYMAYHDHLTKLPNRALFERYFN
HHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
KLKNNTINLNKMAVLFLDLDDFKNVNDTLGHAIGDKLLIQVAKRLKKNLKPSDTLSRLGG
HHCCCCEEEEEEEEEEEECHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCC
DEFTLLLTSIRTREDVIKFASDLSEDFKKPFILDGNKLTIHPSIGISIYPDNGKNLDSLL
CCEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEECCCCEEEECCCCCCHHHHH
KQADNAMYQAKKQKLDFILWR
HHHHHHHHHHHHHCCCEEECC
>Mature Secondary Structure
MNNVIEKHKYYIIIAVFLIIIFLSYNSYQNTTNIIKSKYITHQTLIEKSIINSINNTNYA
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
YQIAEKLLNDKMEKLSRQLISKYRDNPDVLTWDLEKIKQTHKYVNIYIIDNNLKIIHTTY
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHCEEEEEEEECCEEEEEEEH
KKDLGLDFNKYPLFSKVLRKRLEGDNFIVDRMDISIKSGQLKKYSYMPTPDHKYLIELSI
HHHCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEEEEECCCCEEECCCCCCCCCEEEEEEC
NLKEIFPQIDSINLISLTERLKSEYPTIEEINFYRITQQGNIVTESITLSESLLHTDIPD
CHHHHCCCCCCCHHHHHHHHHHHHCCCHHCCEEEEEECCCCEEEEEEEHHHHHHHCCCHH
KIKSLVKKCASTSQVVSSKIKSNSQNRYYSVRFIPCLTQNPEDPYWWDSSVIGIKYNNEF
HHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCCCCEEEEEEECCHH
MLEELNHYKHVFIINMTIITIVFVLFVLVIEYLLKETKYMAYHDHLTKLPNRALFERYFN
HHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
KLKNNTINLNKMAVLFLDLDDFKNVNDTLGHAIGDKLLIQVAKRLKKNLKPSDTLSRLGG
HHCCCCEEEEEEEEEEEECHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCC
DEFTLLLTSIRTREDVIKFASDLSEDFKKPFILDGNKLTIHPSIGISIYPDNGKNLDSLL
CCEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEECCCCEEEECCCCCCHHHHH
KQADNAMYQAKKQKLDFILWR
HHHHHHHHHHHHHCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]