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The map label for this gene is truA [H]

Identifier: 220931093

GI number: 220931093

Start: 252300

End: 253040

Strand: Direct

Name: truA [H]

Synonym: Hore_02450

Alternate gene names: 220931093

Gene position: 252300-253040 (Clockwise)

Preceding gene: 220931092

Following gene: 220931094

Centisome position: 9.79

GC content: 38.33

Gene sequence:

>741_bases
ATGGAGCGAAATATTAAGATAACCGTAGAATATGATGGAACCAACTATAGTGGCTGGCAAATCCAGAATAATACCCCGGA
GACAATACAGGGAGTATTAAGTAAATGTCTTTTTGATATAAATAAGTCGCCAGTTAAATTGATAGGGGCCAGCCGGACAG
ATGCCGGGGTTCATGCCCGGGGCCAGGTGGCCAATTTCAGGCTTAAAGTGGGAATCCCGGTAGAAAGAATTCCCCTTGCT
TTAAACAGTAAACTACCACCTGATATAGTATGTAAAGATGCTGAGGAAGTAACTGAAGATTTTCATGCTCGTTTTGATGC
CAGGGGGAAAAGATATATCTATCGAATTCTTAATAGTGAATATCCATCTCCTTTCTTACGTAATTATACCTATTTTATCC
CCCATAATCTAAATATAGATAAAATGAGAGAGGCAGGTTCGCTTTTAATTGGAGAACATGATTTTGCCTCTTTTCAGGCA
TCCGGTGGATCCAATAAAACCACTGTGAGGACAATAACAAAATTAGATATATATCGTGACCAGTATCAAATAAAAATAGA
AGTATGTGGTAATGGTTTTTTATATAAAATGGTCAGGATAATAGCAGGTACTTTGATTGAAGTCGGGTTAGGGAAAAGAA
CTGTAAAGAGTGTTGGTCAGCTATTGGAGATAAAGGATAGAGATAGAGCAGGTTTTACTGCACCACCCCGGGGGTTAACC
CTTATTGAAGTATTTTATTAA

Upstream 100 bases:

>100_bases
ATTAACTTATATATTTTATTCAATTTATTAAATAGTTTATTAATATTTATATTTCGTTACTGTAACTGTAACCCTGAGAT
GATTCAGGTGGGGGATTGAG

Downstream 100 bases:

>100_bases
AAAAGTCTGTCAGGGTATTATAATATCCTTGACAAAGGGTATTTGTTGTATTAAAATTACTATGAGTTAGTATGTAAAAA
TGCCCCGTTACATTATATTA

Product: pseudouridylate synthase I

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MERNIKITVEYDGTNYSGWQIQNNTPETIQGVLSKCLFDINKSPVKLIGASRTDAGVHARGQVANFRLKVGIPVERIPLA
LNSKLPPDIVCKDAEEVTEDFHARFDARGKRYIYRILNSEYPSPFLRNYTYFIPHNLNIDKMREAGSLLIGEHDFASFQA
SGGSNKTTVRTITKLDIYRDQYQIKIEVCGNGFLYKMVRIIAGTLIEVGLGKRTVKSVGQLLEIKDRDRAGFTAPPRGLT
LIEVFY

Sequences:

>Translated_246_residues
MERNIKITVEYDGTNYSGWQIQNNTPETIQGVLSKCLFDINKSPVKLIGASRTDAGVHARGQVANFRLKVGIPVERIPLA
LNSKLPPDIVCKDAEEVTEDFHARFDARGKRYIYRILNSEYPSPFLRNYTYFIPHNLNIDKMREAGSLLIGEHDFASFQA
SGGSNKTTVRTITKLDIYRDQYQIKIEVCGNGFLYKMVRIIAGTLIEVGLGKRTVKSVGQLLEIKDRDRAGFTAPPRGLT
LIEVFY
>Mature_246_residues
MERNIKITVEYDGTNYSGWQIQNNTPETIQGVLSKCLFDINKSPVKLIGASRTDAGVHARGQVANFRLKVGIPVERIPLA
LNSKLPPDIVCKDAEEVTEDFHARFDARGKRYIYRILNSEYPSPFLRNYTYFIPHNLNIDKMREAGSLLIGEHDFASFQA
SGGSNKTTVRTITKLDIYRDQYQIKIEVCGNGFLYKMVRIIAGTLIEVGLGKRTVKSVGQLLEIKDRDRAGFTAPPRGLT
LIEVFY

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=270, Percent_Identity=30.7407407407407, Blast_Score=104, Evalue=8e-23,
Organism=Escherichia coli, GI1788657, Length=243, Percent_Identity=36.2139917695473, Blast_Score=158, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI71985046, Length=265, Percent_Identity=26.7924528301887, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI116007752, Length=297, Percent_Identity=27.9461279461279, Blast_Score=81, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 27762; Mature: 27762

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERNIKITVEYDGTNYSGWQIQNNTPETIQGVLSKCLFDINKSPVKLIGASRTDAGVHAR
CCCCEEEEEEECCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCC
GQVANFRLKVGIPVERIPLALNSKLPPDIVCKDAEEVTEDFHARFDARGKRYIYRILNSE
CCEEEEEEEECCCHHHCCEEECCCCCCCEEECCHHHHHHHHHHHHCCCCHHHHHEECCCC
YPSPFLRNYTYFIPHNLNIDKMREAGSLLIGEHDFASFQASGGSNKTTVRTITKLDIYRD
CCCHHHHCEEEEECCCCCHHHHHCCCCEEEECCCCCEEECCCCCCCEEEEEEEEHEEECC
QYQIKIEVCGNGFLYKMVRIIAGTLIEVGLGKRTVKSVGQLLEIKDRDRAGFTAPPRGLT
CEEEEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCE
LIEVFY
EEEEEC
>Mature Secondary Structure
MERNIKITVEYDGTNYSGWQIQNNTPETIQGVLSKCLFDINKSPVKLIGASRTDAGVHAR
CCCCEEEEEEECCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCC
GQVANFRLKVGIPVERIPLALNSKLPPDIVCKDAEEVTEDFHARFDARGKRYIYRILNSE
CCEEEEEEEECCCHHHCCEEECCCCCCCEEECCHHHHHHHHHHHHCCCCHHHHHEECCCC
YPSPFLRNYTYFIPHNLNIDKMREAGSLLIGEHDFASFQASGGSNKTTVRTITKLDIYRD
CCCHHHHCEEEEECCCCCHHHHHCCCCEEEECCCCCEEECCCCCCCEEEEEEEEHEEECC
QYQIKIEVCGNGFLYKMVRIIAGTLIEVGLGKRTVKSVGQLLEIKDRDRAGFTAPPRGLT
CEEEEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCE
LIEVFY
EEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA