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The map label for this gene is sdhA [C]

Identifier: 220931060

GI number: 220931060

Start: 219652

End: 221376

Strand: Direct

Name: sdhA [C]

Synonym: Hore_02120

Alternate gene names: 220931060

Gene position: 219652-221376 (Clockwise)

Preceding gene: 220931059

Following gene: 220931061

Centisome position: 8.52

GC content: 45.86

Gene sequence:

>1725_bases
GTGAAGAAAAAGGATATAGAAGTTAAAGTAATGGATACAGATTTGCTGATTATTGGTGGAGGGACGGCCGGGTGCATGGC
TGCCATCAGGGCAAAAGAGTTAGATCCTGATGTAGACGTATTAATTATGGAAAAGGCTCATATAAGACGGAGTGGTTGTC
TGGCCAGTGGTATGAATGCCATCAATGCCTATTTAAATCCGGGTGAAACTCCGGAAAGTTTTGTCAGGTATGTCAGGGCA
GATGCTATGGGGCTTGTTAGAGAGGATTTACTTTTGGGTATGGCCCGGAGATTAAACCAGGTAGTACATAAGGTTGAATC
ATGGGGCCTACCGATTGAAAAGGATAAACAGGGAAATTATGTACCCCGGGGCCGGTGGAATATAAAAATAAACGGGGAAT
CACTCAAACCTATTATTGCCGATAAGGCCATTGAATCCGGAGCCAGGGTTTTAAACAGGGTTACGGTTACCAATCTTATA
ACCACAGAGGATGGAAGTGTTATGGGAGCCATGGGCTTTGGAATTCGTGATGGGGTGTTTTATGTGGTAAAGGCCGGGGC
TACCATAGTGGCTACCGGGGGGGCGGCCGGTATATATAAACCGAATAATGATGGATCAGCCCATCATAAAATGTGGTATT
CCCCCTTTAATACTGGGGCCGGTTATGCAATAGGCATCAGGGCTGGAGCCGAGATGACATCCTTTGAAATGAGGTTTATT
GCCCTGAGGACCAAAGATGTCATTGCTCCGACAGGAACCCTGGCCCTGGGATTTGGGGCAACCCAGGTTAACGCCCGGGG
GGAAAGGTTTATGGCAAAAAAATTCAGACACCTCGGTTCTGAAGGGGCTCCAACTCCCATCAGGGTTTATGGTCCAACCC
GGGAGATAAAAGAAGGAAGAGGTCCGGTTTTTATGGATACCACCCATTTGTCTGAGGAAGAGGTAAGGGATTTAAAGAAA
TCATATTTAAATATGTATCCCGATATTGTTTTGTTCTGGGCTGCAAATAAGATTGATCCCGGCCAGGAACCCCTTGAAAT
CCAGGGTACAGAACCGTACCTGGTAGGGGGGCATGCCCAGGCCGGTTACTGGATTACAGAAAAAAGAGAAACAACCCTGC
CGGGTCTTTATGCAGCCGGTGATGTGGCCGGAGGTGCCCCGTATAAATTTGTCAGTGGAGCCTGGGCTGAAGGAGAAATC
GCTGCCGAAAATGCCCTTGAATATCTCCGCAACAATTATTACAGAGAAGATAAAAAATATGTTGCCCAGGTTATCGAACG
GGAAATAATGAGGGTATATGCCCCGCTGAGGAGAAGGGATGGGGTTAAGGCTGAGGAAATGGAAGAACGACTCCAGAAAA
TAATGGACCAGTATGCTGGAGGGGTAAGTCGGTATTATGAGATGAATGAAGATGAGTTAAAACTGGCCCGAAAATTACTT
AAAAAGATGCGGGGTCAGGTAAAATATCTCAGAGCTGAAGACTACCATAAGCTAATGCTCTGTCATGAAGTGCTTGATAG
ACTGGATGTTGCCTCAGTTCTGGTTGAACACCTTTTATATAGACGGGAAACCAGGTGGCCTGCCTATCAGAGCAGAGTTG
ATTACCCGGATAGAAATGATCGGGAGTGGTTAAAGTTTGTAAATAGTCGACTTGAAGGTAATAAAGTTAGTATTATAGAA
AGGCCCTATAAACAGATAGTTCCTGGAGACAGGTATTTACCCTGA

Upstream 100 bases:

>100_bases
GATAATTTCAAACAATAGGGCTGGCCTTTAAGATTTTAACAAATTACAGAATTTATTCATAATATATTAAGGTGATGAAA
TTAGTTACGGGGGGATGATT

Downstream 100 bases:

>100_bases
GGGGTAGCCAGAATAATTTTAGATAAGGAGGTTAAAACTATGGGTATCAGGATAGATAAAGGGAAGTGCACGGGATGTCC
GGGAAGGGATGAACCACCCT

Product: adenylylsulfate reductase subunit alpha

Products: Adenylylsulfate; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MKKKDIEVKVMDTDLLIIGGGTAGCMAAIRAKELDPDVDVLIMEKAHIRRSGCLASGMNAINAYLNPGETPESFVRYVRA
DAMGLVREDLLLGMARRLNQVVHKVESWGLPIEKDKQGNYVPRGRWNIKINGESLKPIIADKAIESGARVLNRVTVTNLI
TTEDGSVMGAMGFGIRDGVFYVVKAGATIVATGGAAGIYKPNNDGSAHHKMWYSPFNTGAGYAIGIRAGAEMTSFEMRFI
ALRTKDVIAPTGTLALGFGATQVNARGERFMAKKFRHLGSEGAPTPIRVYGPTREIKEGRGPVFMDTTHLSEEEVRDLKK
SYLNMYPDIVLFWAANKIDPGQEPLEIQGTEPYLVGGHAQAGYWITEKRETTLPGLYAAGDVAGGAPYKFVSGAWAEGEI
AAENALEYLRNNYYREDKKYVAQVIEREIMRVYAPLRRRDGVKAEEMEERLQKIMDQYAGGVSRYYEMNEDELKLARKLL
KKMRGQVKYLRAEDYHKLMLCHEVLDRLDVASVLVEHLLYRRETRWPAYQSRVDYPDRNDREWLKFVNSRLEGNKVSIIE
RPYKQIVPGDRYLP

Sequences:

>Translated_574_residues
MKKKDIEVKVMDTDLLIIGGGTAGCMAAIRAKELDPDVDVLIMEKAHIRRSGCLASGMNAINAYLNPGETPESFVRYVRA
DAMGLVREDLLLGMARRLNQVVHKVESWGLPIEKDKQGNYVPRGRWNIKINGESLKPIIADKAIESGARVLNRVTVTNLI
TTEDGSVMGAMGFGIRDGVFYVVKAGATIVATGGAAGIYKPNNDGSAHHKMWYSPFNTGAGYAIGIRAGAEMTSFEMRFI
ALRTKDVIAPTGTLALGFGATQVNARGERFMAKKFRHLGSEGAPTPIRVYGPTREIKEGRGPVFMDTTHLSEEEVRDLKK
SYLNMYPDIVLFWAANKIDPGQEPLEIQGTEPYLVGGHAQAGYWITEKRETTLPGLYAAGDVAGGAPYKFVSGAWAEGEI
AAENALEYLRNNYYREDKKYVAQVIEREIMRVYAPLRRRDGVKAEEMEERLQKIMDQYAGGVSRYYEMNEDELKLARKLL
KKMRGQVKYLRAEDYHKLMLCHEVLDRLDVASVLVEHLLYRRETRWPAYQSRVDYPDRNDREWLKFVNSRLEGNKVSIIE
RPYKQIVPGDRYLP
>Mature_574_residues
MKKKDIEVKVMDTDLLIIGGGTAGCMAAIRAKELDPDVDVLIMEKAHIRRSGCLASGMNAINAYLNPGETPESFVRYVRA
DAMGLVREDLLLGMARRLNQVVHKVESWGLPIEKDKQGNYVPRGRWNIKINGESLKPIIADKAIESGARVLNRVTVTNLI
TTEDGSVMGAMGFGIRDGVFYVVKAGATIVATGGAAGIYKPNNDGSAHHKMWYSPFNTGAGYAIGIRAGAEMTSFEMRFI
ALRTKDVIAPTGTLALGFGATQVNARGERFMAKKFRHLGSEGAPTPIRVYGPTREIKEGRGPVFMDTTHLSEEEVRDLKK
SYLNMYPDIVLFWAANKIDPGQEPLEIQGTEPYLVGGHAQAGYWITEKRETTLPGLYAAGDVAGGAPYKFVSGAWAEGEI
AAENALEYLRNNYYREDKKYVAQVIEREIMRVYAPLRRRDGVKAEEMEERLQKIMDQYAGGVSRYYEMNEDELKLARKLL
KKMRGQVKYLRAEDYHKLMLCHEVLDRLDVASVLVEHLLYRRETRWPAYQSRVDYPDRNDREWLKFVNSRLEGNKVSIIE
RPYKQIVPGDRYLP

Specific function: Two Distinct, Membrane-Bound, FAD-Containing Enzymes Are Responsible For The Catalysis Of Fumarate And Succinate Interconversion; The Fumarate Reductase Is Used In Anaerobic Growth, And The Succinate Dehydrogenase Is Used In Aerobic Growth. [C]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786942, Length=452, Percent_Identity=25.2212389380531, Blast_Score=80, Evalue=5e-16,

Paralogues:

None

Copy number: 1900 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1360 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1100 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: 1.8.99.2

Molecular weight: Translated: 64530; Mature: 64530

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKDIEVKVMDTDLLIIGGGTAGCMAAIRAKELDPDVDVLIMEKAHIRRSGCLASGMNA
CCCCCEEEEEEECEEEEECCCHHHHHHHHHHHCCCCCCCEEEECHHHHHHHCCHHHCHHH
INAYLNPGETPESFVRYVRADAMGLVREDLLLGMARRLNQVVHKVESWGLPIEKDKQGNY
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
VPRGRWNIKINGESLKPIIADKAIESGARVLNRVTVTNLITTEDGSVMGAMGFGIRDGVF
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEECCCCCCCCEE
YVVKAGATIVATGGAAGIYKPNNDGSAHHKMWYSPFNTGAGYAIGIRAGAEMTSFEMRFI
EEEECCCEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEECCCCCHHEEEEEE
ALRTKDVIAPTGTLALGFGATQVNARGERFMAKKFRHLGSEGAPTPIRVYGPTREIKEGR
EEECCCCCCCCCCEEEECCCEEECCCHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHCCC
GPVFMDTTHLSEEEVRDLKKSYLNMYPDIVLFWAANKIDPGQEPLEIQGTEPYLVGGHAQ
CCEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEECCCCEEECCCCC
AGYWITEKRETTLPGLYAAGDVAGGAPYKFVSGAWAEGEIAAENALEYLRNNYYREDKKY
CCEEEECCCCCCCCCEEECCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VAQVIEREIMRVYAPLRRRDGVKAEEMEERLQKIMDQYAGGVSRYYEMNEDELKLARKLL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHH
KKMRGQVKYLRAEDYHKLMLCHEVLDRLDVASVLVEHLLYRRETRWPAYQSRVDYPDRND
HHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCH
REWLKFVNSRLEGNKVSIIERPYKQIVPGDRYLP
HHHHHHHHHHCCCCEEEEEECCHHHCCCCCCCCC
>Mature Secondary Structure
MKKKDIEVKVMDTDLLIIGGGTAGCMAAIRAKELDPDVDVLIMEKAHIRRSGCLASGMNA
CCCCCEEEEEEECEEEEECCCHHHHHHHHHHHCCCCCCCEEEECHHHHHHHCCHHHCHHH
INAYLNPGETPESFVRYVRADAMGLVREDLLLGMARRLNQVVHKVESWGLPIEKDKQGNY
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
VPRGRWNIKINGESLKPIIADKAIESGARVLNRVTVTNLITTEDGSVMGAMGFGIRDGVF
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEECCCCCCCCEE
YVVKAGATIVATGGAAGIYKPNNDGSAHHKMWYSPFNTGAGYAIGIRAGAEMTSFEMRFI
EEEECCCEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEECCCCCHHEEEEEE
ALRTKDVIAPTGTLALGFGATQVNARGERFMAKKFRHLGSEGAPTPIRVYGPTREIKEGR
EEECCCCCCCCCCEEEECCCEEECCCHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHCCC
GPVFMDTTHLSEEEVRDLKKSYLNMYPDIVLFWAANKIDPGQEPLEIQGTEPYLVGGHAQ
CCEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEECCCCEEECCCCC
AGYWITEKRETTLPGLYAAGDVAGGAPYKFVSGAWAEGEIAAENALEYLRNNYYREDKKY
CCEEEECCCCCCCCCEEECCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VAQVIEREIMRVYAPLRRRDGVKAEEMEERLQKIMDQYAGGVSRYYEMNEDELKLARKLL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHH
KKMRGQVKYLRAEDYHKLMLCHEVLDRLDVASVLVEHLLYRRETRWPAYQSRVDYPDRND
HHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCH
REWLKFVNSRLEGNKVSIIERPYKQIVPGDRYLP
HHHHHHHHHHCCCCEEEEEECCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: AMP; sulfite; acceptor

Specific reaction: AMP + sulfite + acceptor = adenylyl sulfate + reduced acceptor

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]