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The map label for this gene is guaA

Identifier: 220931051

GI number: 220931051

Start: 207995

End: 209527

Strand: Direct

Name: guaA

Synonym: Hore_02020

Alternate gene names: 220931051

Gene position: 207995-209527 (Clockwise)

Preceding gene: 220931050

Following gene: 220931052

Centisome position: 8.07

GC content: 42.14

Gene sequence:

>1533_bases
ATGAAAGAAGATAAAATTTTAGTACTTGATTTCGGTGGCCAGTACAGTCAATTAATTGCTAGACGGGTAAGGGAATGTAA
TGTTTATTCCATTATCAAGCCATATAATATCTCTATAGACCAGATTAAAGATATAAATCCGGCCGGAATTATCTTTTCCG
GGGGTCCCAAGAGTGTTACCGGGCCAGAGGCTCCCATGATTGATAAAGAAGTTTTTAAGTTAAATATACCCATTCTTGGC
ATCTGCTACGGTATGCAGTTAATGACTGCATTGCTACCCGGTGGGGCTGTTGAAAAGGCCAGGCACGGGGAATATGGAAA
GGCCAGGTTACATATTACTGAAAAGGATAAATTATTTGAGGGTATTTCTGATAGTCAGGTCTGGATGAGTCACTGGGACC
GTGTTAAGAAGGTTCCCGACGGATTTAAAGTAACCGCCAAAACTGATATTACGCCGGTAGCAGCTATGGGTAATCCTGAA
CTTAAGTTTTACGGTGTTCAATTTCATCCGGAAGTTGTTCATACCATCCAGGGTTTAAACATTATTCGCAATTATTTATT
TAAAGTTTGTGGGGTTAAAGGTACCTGGAATATGGCTGATTTTATCAATGAGGAAATTGATAGGATAAAGGAGGAAGTAG
GTTCGAGTCAGGTTATTTCTGGCCTGTCCGGTGGGGTTGATTCTGCTGTGGCTTCAACCATGGTTCACCGGGCAATCGGG
GATCAGCTGACCTGTATTTTTGTAGACCATGGTCTTTTGAGAAAGGGAGAAGCAGAACAGGTAAAACGGACATTTGTAGA
TGAGTTTAATATTCCCCTTGTTTACGTGGATGCCCGGGAACGGTTTTTAAACAGGCTTAATGGCGTTAAGGACCCTGAAA
CTAAACGAAAGATAATAGGGGAGGAGTTTATAAGGGTTTTTGAAGAGGAGGCCAGAAAACTTGGTGATGCCAGATATCTG
GTCCAGGGAACCCTGTATTCTGATGTAATTGAAAGCGGTAGTGAAACTGCTGAAACTATAAAAAGCCACCATAATGTCGG
GGGTTTACCTGACAACATGGACTTTAAATTAATTGAACCCCTCAGGAACCTCTTTAAGGATGAGGTCCGGAAAATAGGTG
AGGTTCTGGGTCTCCCTGATGAGATAGTCTGGCGGCAGCCTTTTCCTGGTCCGGGTCTGGCTATAAGAATAATCGGTGAA
ATTGATGGTAAAAAACTTGAAATCCTGAGAAAAGCAGATTCCATCATCCAGGAAGAGATAAAAGAGGCCGGGTATTATAA
AGAAATCTGGCAGTCTTTTGCTGTTCTCCCTGATTTGAAAAGTGTTGGTGTTATGGGAGATACAAGGACCTATGGTTATC
CTATTATCTTAAGGGCTGTTACCAGTGATGATGCCATGACAGCAGACTGGGCCAGATTACCGTATGAGTTGCTGGAGAAA
ATATCAACCCGGATAGTAAATGAAGTTGACTCGGTTAACAGGGTTGTCTATGACATTACCAGTAAACCTCCAGCAACTAT
AGAATGGGAATAA

Upstream 100 bases:

>100_bases
AGGAAGGGGAAAATATATTTGTCCATAATTCCTTGACATAGGTTATTAAGATAGATATACTTTAATCAAGTGAAGTAAAG
AAGGATGAAAGGGGAGTTAT

Downstream 100 bases:

>100_bases
CCAGGAGGGGGAGAATTATGGGGAAGGATATTAAAGACCGATTTACAGAACAATTATTTGATGCTATTTTACTTCTGGAA
AACAAAGAAGAGTGTTATCG

Product: GMP synthase

Products: NA

Alternate protein names: GMP synthetase; Glutamine amidotransferase

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MKEDKILVLDFGGQYSQLIARRVRECNVYSIIKPYNISIDQIKDINPAGIIFSGGPKSVTGPEAPMIDKEVFKLNIPILG
ICYGMQLMTALLPGGAVEKARHGEYGKARLHITEKDKLFEGISDSQVWMSHWDRVKKVPDGFKVTAKTDITPVAAMGNPE
LKFYGVQFHPEVVHTIQGLNIIRNYLFKVCGVKGTWNMADFINEEIDRIKEEVGSSQVISGLSGGVDSAVASTMVHRAIG
DQLTCIFVDHGLLRKGEAEQVKRTFVDEFNIPLVYVDARERFLNRLNGVKDPETKRKIIGEEFIRVFEEEARKLGDARYL
VQGTLYSDVIESGSETAETIKSHHNVGGLPDNMDFKLIEPLRNLFKDEVRKIGEVLGLPDEIVWRQPFPGPGLAIRIIGE
IDGKKLEILRKADSIIQEEIKEAGYYKEIWQSFAVLPDLKSVGVMGDTRTYGYPIILRAVTSDDAMTADWARLPYELLEK
ISTRIVNEVDSVNRVVYDITSKPPATIEWE

Sequences:

>Translated_510_residues
MKEDKILVLDFGGQYSQLIARRVRECNVYSIIKPYNISIDQIKDINPAGIIFSGGPKSVTGPEAPMIDKEVFKLNIPILG
ICYGMQLMTALLPGGAVEKARHGEYGKARLHITEKDKLFEGISDSQVWMSHWDRVKKVPDGFKVTAKTDITPVAAMGNPE
LKFYGVQFHPEVVHTIQGLNIIRNYLFKVCGVKGTWNMADFINEEIDRIKEEVGSSQVISGLSGGVDSAVASTMVHRAIG
DQLTCIFVDHGLLRKGEAEQVKRTFVDEFNIPLVYVDARERFLNRLNGVKDPETKRKIIGEEFIRVFEEEARKLGDARYL
VQGTLYSDVIESGSETAETIKSHHNVGGLPDNMDFKLIEPLRNLFKDEVRKIGEVLGLPDEIVWRQPFPGPGLAIRIIGE
IDGKKLEILRKADSIIQEEIKEAGYYKEIWQSFAVLPDLKSVGVMGDTRTYGYPIILRAVTSDDAMTADWARLPYELLEK
ISTRIVNEVDSVNRVVYDITSKPPATIEWE
>Mature_510_residues
MKEDKILVLDFGGQYSQLIARRVRECNVYSIIKPYNISIDQIKDINPAGIIFSGGPKSVTGPEAPMIDKEVFKLNIPILG
ICYGMQLMTALLPGGAVEKARHGEYGKARLHITEKDKLFEGISDSQVWMSHWDRVKKVPDGFKVTAKTDITPVAAMGNPE
LKFYGVQFHPEVVHTIQGLNIIRNYLFKVCGVKGTWNMADFINEEIDRIKEEVGSSQVISGLSGGVDSAVASTMVHRAIG
DQLTCIFVDHGLLRKGEAEQVKRTFVDEFNIPLVYVDARERFLNRLNGVKDPETKRKIIGEEFIRVFEEEARKLGDARYL
VQGTLYSDVIESGSETAETIKSHHNVGGLPDNMDFKLIEPLRNLFKDEVRKIGEVLGLPDEIVWRQPFPGPGLAIRIIGE
IDGKKLEILRKADSIIQEEIKEAGYYKEIWQSFAVLPDLKSVGVMGDTRTYGYPIILRAVTSDDAMTADWARLPYELLEK
ISTRIVNEVDSVNRVVYDITSKPPATIEWE

Specific function: Catalyzes the synthesis of GMP from XMP

COG id: COG0519

COG function: function code F; GMP synthase, PP-ATPase domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GMP-binding domain

Homologues:

Organism=Homo sapiens, GI4504035, Length=551, Percent_Identity=36.4791288566243, Blast_Score=290, Evalue=2e-78,
Organism=Escherichia coli, GI1788854, Length=522, Percent_Identity=50.1915708812261, Blast_Score=541, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI71992710, Length=570, Percent_Identity=35.9649122807018, Blast_Score=297, Evalue=8e-81,
Organism=Caenorhabditis elegans, GI71992717, Length=570, Percent_Identity=35.9649122807018, Blast_Score=297, Evalue=9e-81,
Organism=Caenorhabditis elegans, GI133901714, Length=570, Percent_Identity=35.9649122807018, Blast_Score=297, Evalue=1e-80,
Organism=Saccharomyces cerevisiae, GI6323873, Length=516, Percent_Identity=52.9069767441861, Blast_Score=538, Evalue=1e-154,
Organism=Drosophila melanogaster, GI281365319, Length=550, Percent_Identity=36.7272727272727, Blast_Score=288, Evalue=4e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GUAA_HALOH (B8D0Z5)

Other databases:

- EMBL:   CP001098
- RefSeq:   YP_002507959.1
- ProteinModelPortal:   B8D0Z5
- SMR:   B8D0Z5
- GeneID:   7312521
- GenomeReviews:   CP001098_GR
- KEGG:   hor:Hore_02020
- HOGENOM:   HBG391775
- OMA:   MKLVEPL
- HAMAP:   MF_00344
- InterPro:   IPR006220
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR001674
- InterPro:   IPR004739
- InterPro:   IPR022955
- InterPro:   IPR022310
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR00097
- PRINTS:   PR00096
- TIGRFAMs:   TIGR00884
- TIGRFAMs:   TIGR00888

Pfam domain/function: PF00117 GATase; PF00958 GMP_synt_C; PF02540 NAD_synthase

EC number: =6.3.5.2

Molecular weight: Translated: 57286; Mature: 57286

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 82-82 ACT_SITE 169-169 ACT_SITE 171-171

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEDKILVLDFGGQYSQLIARRVRECNVYSIIKPYNISIDQIKDINPAGIIFSGGPKSVT
CCCCCEEEEECCCCHHHHHHHHHHHCCEEEEECCCCCCHHHHCCCCCCCEEECCCCCCCC
GPEAPMIDKEVFKLNIPILGICYGMQLMTALLPGGAVEKARHGEYGKARLHITEKDKLFE
CCCCCCCCCHHEEECCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEECHHHHHC
GISDSQVWMSHWDRVKKVPDGFKVTAKTDITPVAAMGNPELKFYGVQFHPEVVHTIQGLN
CCCCCHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCEEEEEEEECHHHHHHHHHHH
IIRNYLFKVCGVKGTWNMADFINEEIDRIKEEVGSSQVISGLSGGVDSAVASTMVHRAIG
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHC
DQLTCIFVDHGLLRKGEAEQVKRTFVDEFNIPLVYVDARERFLNRLNGVKDPETKRKIIG
CCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEECHHHHHHHHHCCCCCCHHHHHHHH
EEFIRVFEEEARKLGDARYLVQGTLYSDVIESGSETAETIKSHHNVGGLPDNMDFKLIEP
HHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHHH
LRNLFKDEVRKIGEVLGLPDEIVWRQPFPGPGLAIRIIGEIDGKKLEILRKADSIIQEEI
HHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHH
KEAGYYKEIWQSFAVLPDLKSVGVMGDTRTYGYPIILRAVTSDDAMTADWARLPYELLEK
HHCCHHHHHHHHHHCCCCHHHCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHCCHHHHHH
ISTRIVNEVDSVNRVVYDITSKPPATIEWE
HHHHHHHHHHHHHHEEEEECCCCCCCEECC
>Mature Secondary Structure
MKEDKILVLDFGGQYSQLIARRVRECNVYSIIKPYNISIDQIKDINPAGIIFSGGPKSVT
CCCCCEEEEECCCCHHHHHHHHHHHCCEEEEECCCCCCHHHHCCCCCCCEEECCCCCCCC
GPEAPMIDKEVFKLNIPILGICYGMQLMTALLPGGAVEKARHGEYGKARLHITEKDKLFE
CCCCCCCCCHHEEECCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEECHHHHHC
GISDSQVWMSHWDRVKKVPDGFKVTAKTDITPVAAMGNPELKFYGVQFHPEVVHTIQGLN
CCCCCHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCEEEEEEEECHHHHHHHHHHH
IIRNYLFKVCGVKGTWNMADFINEEIDRIKEEVGSSQVISGLSGGVDSAVASTMVHRAIG
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHC
DQLTCIFVDHGLLRKGEAEQVKRTFVDEFNIPLVYVDARERFLNRLNGVKDPETKRKIIG
CCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEECHHHHHHHHHCCCCCCHHHHHHHH
EEFIRVFEEEARKLGDARYLVQGTLYSDVIESGSETAETIKSHHNVGGLPDNMDFKLIEP
HHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHHH
LRNLFKDEVRKIGEVLGLPDEIVWRQPFPGPGLAIRIIGEIDGKKLEILRKADSIIQEEI
HHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHH
KEAGYYKEIWQSFAVLPDLKSVGVMGDTRTYGYPIILRAVTSDDAMTADWARLPYELLEK
HHCCHHHHHHHHHHCCCCHHHCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHCCHHHHHH
ISTRIVNEVDSVNRVVYDITSKPPATIEWE
HHHHHHHHHHHHHHEEEEECCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA