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The map label for this gene is groL

Identifier: 220931050

GI number: 220931050

Start: 206110

End: 207753

Strand: Direct

Name: groL

Synonym: Hore_02010

Alternate gene names: 220931050

Gene position: 206110-207753 (Clockwise)

Preceding gene: 220931049

Following gene: 220931051

Centisome position: 7.99

GC content: 44.34

Gene sequence:

>1644_bases
ATGGCAAAAGAATTAAAATTTAGTGAAGATGCTCGCCGTGCTCTGGAACGCGGTGTTGATACTCTGGCAAATGCTGTTAA
AGTAACTTTAGGTCCAAAAGGACGAAATGTAGTTCTTGAAAAGAGCTTTGGAGCTCCTACTATCACCAACGATGGTGTTA
GTATTGCCCGTGAAATAGAACTTGAAAATCACTACGAAAACATGGGGGCTCAGACTGTAAAAGAGGTTGCTACCAAAACC
AATGATGTTGCCGGTGATGGTACAACCACTGCTACAGTACTGGCTCAGGCTATTTTCAAGGAAGGTTTAAAGAATGTGGC
CGCCGGTGCCAACCCCATGATCCTGAAAAGGGGTATTGAAAAGGCCGTTCAGAAGCTGGTAGAAGAGATTAAGGAACTAA
GCAAACCTGTTGAAGGAAAAGAAGCAGTTTCCCAGGTTGCTGCTATTTCTGCCGGTAATGATGAAGAAGTCGGTAAGCTT
ATTGCTGAAGCTATGGAGAAAGTTGGTCAGGATGGAGTTATCTCTGTTGAAGAATCCAAGAGTATGGGGACTTCTTTAGA
TGTAGTTGAAGGTATGCAGTTCGATAGAGGATATCTCTCCCCCTATATGGTAACCGATACTGATGCTATGGAAGCTTCCC
TTGAAGATCCCTATATCCTGATCACTGACAAGAAGATATCTAATATCCAGGAAATCTTACCCCTGTTAGAAAAAGTAGCC
CAGAGTGGTAAACCTCTCTTAATAATTGCTGAAGATGTTGAAGGGGAAGCCCTGGCTACTCTTGTTGTCAACAAGATTCG
TGGTACCTTTAACTGTGTTGCTGTTAAAGCACCTGGCTTTGGTGATCGTCGTAAGGCTATGTTAGAAGACATTGCTATTC
TGACCGGTGGTCAGGTAATCACTGAAGACCTGGGTCTCAAGCTCGAAAATGCTGATATTAGTATGCTTGGTCGGGCCCAC
AAAGTAACAGTAACCAAAGAGGATACTACTATTGTAGAAGGTGCTGGAGATAGCAAAGAAATTCAGAATAGAATTAAGCA
GATCAGGACTCAAATTGAAAATACTGATTCTGATTTTGACAGGGAAAAACTGCAGGAAAGACTGGCTAAACTGGCCGGTG
GTGTGGCTGTAATTAAGGTTGGTGCTGCTACTGAAACTGAATTAAAAGAAAAGAAACACCGTATTGAAGATGCTCTCTCT
GCTACCAGGGCCGCTGTAGAAGAAGGACTGGTAGCCGGTGGTGGAACCACCCTTATTGATGCCATTCCTGCCCTTGATGA
ACTGAACCTTGAAGGTGACGAAGCTACCGGTGTTGACATTGTTAGAAAAGCACTTGAAGCCCCGGTACGTCTCATAGCAG
ACAATGCCGGTTATGAAGGTTCAGTAATTGTTGAGAAGGTTAAGTCTGAAGATAAAGGTATCGGTTTCGATGCCTATAAC
GGTGAGTTTGTAAATATGATTGAATCCGGTATTGTAGACCCGGCTAAGGTAACCCGTTCTGCCCTTCAGAATGCTGCCAG
TGCTGCTGCTATGTTGCTGACTACTGAATGCCTGGTGGCTGATAAAGAAGAGGATAATGACAGTAATGGTAATGCCGGAA
TGCCCGGTGGCGGTATGCCCGGCGGAATGGGTGGCATGATGTAA

Upstream 100 bases:

>100_bases
TAGACGGTGAAGATTATATCATTTTAAATCTGGAGGATGTATTGGCAGTAATTGAAAAATAATCTGAAAACCCTAAACTA
CTTAAGGAGGGATACATAAA

Downstream 100 bases:

>100_bases
GAAGCCTTTTCCCTAAACAAATGGCAGCGTACCTTTTAAAGGTACGCTGCTTTTTTTTATGTCAAAGTCAATTAGTCTGC
CCGAAAAATCTTGCATGGAA

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein; Protein Cpn60

Number of amino acids: Translated: 547; Mature: 546

Protein sequence:

>547_residues
MAKELKFSEDARRALERGVDTLANAVKVTLGPKGRNVVLEKSFGAPTITNDGVSIAREIELENHYENMGAQTVKEVATKT
NDVAGDGTTTATVLAQAIFKEGLKNVAAGANPMILKRGIEKAVQKLVEEIKELSKPVEGKEAVSQVAAISAGNDEEVGKL
IAEAMEKVGQDGVISVEESKSMGTSLDVVEGMQFDRGYLSPYMVTDTDAMEASLEDPYILITDKKISNIQEILPLLEKVA
QSGKPLLIIAEDVEGEALATLVVNKIRGTFNCVAVKAPGFGDRRKAMLEDIAILTGGQVITEDLGLKLENADISMLGRAH
KVTVTKEDTTIVEGAGDSKEIQNRIKQIRTQIENTDSDFDREKLQERLAKLAGGVAVIKVGAATETELKEKKHRIEDALS
ATRAAVEEGLVAGGGTTLIDAIPALDELNLEGDEATGVDIVRKALEAPVRLIADNAGYEGSVIVEKVKSEDKGIGFDAYN
GEFVNMIESGIVDPAKVTRSALQNAASAAAMLLTTECLVADKEEDNDSNGNAGMPGGGMPGGMGGMM

Sequences:

>Translated_547_residues
MAKELKFSEDARRALERGVDTLANAVKVTLGPKGRNVVLEKSFGAPTITNDGVSIAREIELENHYENMGAQTVKEVATKT
NDVAGDGTTTATVLAQAIFKEGLKNVAAGANPMILKRGIEKAVQKLVEEIKELSKPVEGKEAVSQVAAISAGNDEEVGKL
IAEAMEKVGQDGVISVEESKSMGTSLDVVEGMQFDRGYLSPYMVTDTDAMEASLEDPYILITDKKISNIQEILPLLEKVA
QSGKPLLIIAEDVEGEALATLVVNKIRGTFNCVAVKAPGFGDRRKAMLEDIAILTGGQVITEDLGLKLENADISMLGRAH
KVTVTKEDTTIVEGAGDSKEIQNRIKQIRTQIENTDSDFDREKLQERLAKLAGGVAVIKVGAATETELKEKKHRIEDALS
ATRAAVEEGLVAGGGTTLIDAIPALDELNLEGDEATGVDIVRKALEAPVRLIADNAGYEGSVIVEKVKSEDKGIGFDAYN
GEFVNMIESGIVDPAKVTRSALQNAASAAAMLLTTECLVADKEEDNDSNGNAGMPGGGMPGGMGGMM
>Mature_546_residues
AKELKFSEDARRALERGVDTLANAVKVTLGPKGRNVVLEKSFGAPTITNDGVSIAREIELENHYENMGAQTVKEVATKTN
DVAGDGTTTATVLAQAIFKEGLKNVAAGANPMILKRGIEKAVQKLVEEIKELSKPVEGKEAVSQVAAISAGNDEEVGKLI
AEAMEKVGQDGVISVEESKSMGTSLDVVEGMQFDRGYLSPYMVTDTDAMEASLEDPYILITDKKISNIQEILPLLEKVAQ
SGKPLLIIAEDVEGEALATLVVNKIRGTFNCVAVKAPGFGDRRKAMLEDIAILTGGQVITEDLGLKLENADISMLGRAHK
VTVTKEDTTIVEGAGDSKEIQNRIKQIRTQIENTDSDFDREKLQERLAKLAGGVAVIKVGAATETELKEKKHRIEDALSA
TRAAVEEGLVAGGGTTLIDAIPALDELNLEGDEATGVDIVRKALEAPVRLIADNAGYEGSVIVEKVKSEDKGIGFDAYNG
EFVNMIESGIVDPAKVTRSALQNAASAAAMLLTTECLVADKEEDNDSNGNAGMPGGGMPGGMGGMM

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family

Homologues:

Organism=Homo sapiens, GI41399285, Length=529, Percent_Identity=50.2835538752363, Blast_Score=528, Evalue=1e-150,
Organism=Homo sapiens, GI31542947, Length=529, Percent_Identity=50.2835538752363, Blast_Score=528, Evalue=1e-150,
Organism=Homo sapiens, GI58331173, Length=554, Percent_Identity=22.5631768953069, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI38455427, Length=118, Percent_Identity=31.3559322033898, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI302058290, Length=518, Percent_Identity=23.5521235521236, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI5453607, Length=128, Percent_Identity=32.03125, Blast_Score=66, Evalue=8e-11,
Organism=Escherichia coli, GI1790586, Length=521, Percent_Identity=64.1074856046065, Blast_Score=647, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17555558, Length=529, Percent_Identity=48.7712665406427, Blast_Score=504, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI193210679, Length=210, Percent_Identity=50.4761904761905, Blast_Score=201, Evalue=6e-52,
Organism=Caenorhabditis elegans, GI17564182, Length=545, Percent_Identity=22.2018348623853, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI25144674, Length=174, Percent_Identity=28.1609195402299, Blast_Score=73, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI32566944, Length=118, Percent_Identity=32.2033898305085, Blast_Score=65, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6323288, Length=524, Percent_Identity=53.8167938931298, Blast_Score=543, Evalue=1e-155,
Organism=Saccharomyces cerevisiae, GI6322350, Length=556, Percent_Identity=24.1007194244604, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6322524, Length=135, Percent_Identity=30.3703703703704, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24641193, Length=522, Percent_Identity=49.0421455938697, Blast_Score=517, Evalue=1e-147,
Organism=Drosophila melanogaster, GI24641191, Length=522, Percent_Identity=49.0421455938697, Blast_Score=517, Evalue=1e-147,
Organism=Drosophila melanogaster, GI45550936, Length=522, Percent_Identity=46.7432950191571, Blast_Score=490, Evalue=1e-139,
Organism=Drosophila melanogaster, GI45550132, Length=522, Percent_Identity=46.7432950191571, Blast_Score=490, Evalue=1e-139,
Organism=Drosophila melanogaster, GI45550935, Length=522, Percent_Identity=46.7432950191571, Blast_Score=490, Evalue=1e-139,
Organism=Drosophila melanogaster, GI17864606, Length=552, Percent_Identity=42.0289855072464, Blast_Score=441, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24584129, Length=528, Percent_Identity=36.3636363636364, Blast_Score=314, Evalue=8e-86,
Organism=Drosophila melanogaster, GI19921262, Length=528, Percent_Identity=36.3636363636364, Blast_Score=314, Evalue=8e-86,
Organism=Drosophila melanogaster, GI24645179, Length=535, Percent_Identity=22.803738317757, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): CH60_HALOH (B8D0Z4)

Other databases:

- EMBL:   CP001098
- RefSeq:   YP_002507958.1
- ProteinModelPortal:   B8D0Z4
- SMR:   B8D0Z4
- GeneID:   7312520
- GenomeReviews:   CP001098_GR
- KEGG:   hor:Hore_02010
- HOGENOM:   HBG625289
- OMA:   EIDTKEQ
- ProtClustDB:   CLSK2806181
- GO:   GO:0005737
- HAMAP:   MF_00600
- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423
- PANTHER:   PTHR11353
- PRINTS:   PR00298
- TIGRFAMs:   TIGR02348

Pfam domain/function: PF00118 Cpn60_TCP1; SSF48592 GroEL-ATPase

EC number: NA

Molecular weight: Translated: 57967; Mature: 57835

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKELKFSEDARRALERGVDTLANAVKVTLGPKGRNVVLEKSFGAPTITNDGVSIAREIE
CCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCCCCCCCCCCCHHHHHHH
LENHYENMGAQTVKEVATKTNDVAGDGTTTATVLAQAIFKEGLKNVAAGANPMILKRGIE
HHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
KAVQKLVEEIKELSKPVEGKEAVSQVAAISAGNDEEVGKLIAEAMEKVGQDGVISVEESK
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEECCC
SMGTSLDVVEGMQFDRGYLSPYMVTDTDAMEASLEDPYILITDKKISNIQEILPLLEKVA
CCCCCHHHHHCCCCCCCCCCCEEEECCHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHH
QSGKPLLIIAEDVEGEALATLVVNKIRGTFNCVAVKAPGFGDRRKAMLEDIAILTGGQVI
HCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCHH
TEDLGLKLENADISMLGRAHKVTVTKEDTTIVEGAGDSKEIQNRIKQIRTQIENTDSDFD
HHHHCCEECCCCHHHHCCCEEEEEEECCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCC
REKLQERLAKLAGGVAVIKVGAATETELKEKKHRIEDALSATRAAVEEGLVAGGGTTLID
HHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
AIPALDELNLEGDEATGVDIVRKALEAPVRLIADNAGYEGSVIVEKVKSEDKGIGFDAYN
HHCCHHHCCCCCCCCCCHHHHHHHHHCCHHEEECCCCCCCHHHHHHHHCCCCCCCCCCCC
GEFVNMIESGIVDPAKVTRSALQNAASAAAMLLTTECLVADKEEDNDSNGNAGMPGGGMP
CHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
GGMGGMM
CCCCCCC
>Mature Secondary Structure 
AKELKFSEDARRALERGVDTLANAVKVTLGPKGRNVVLEKSFGAPTITNDGVSIAREIE
CCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCCCCCCCCCCCHHHHHHH
LENHYENMGAQTVKEVATKTNDVAGDGTTTATVLAQAIFKEGLKNVAAGANPMILKRGIE
HHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
KAVQKLVEEIKELSKPVEGKEAVSQVAAISAGNDEEVGKLIAEAMEKVGQDGVISVEESK
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEECCC
SMGTSLDVVEGMQFDRGYLSPYMVTDTDAMEASLEDPYILITDKKISNIQEILPLLEKVA
CCCCCHHHHHCCCCCCCCCCCEEEECCHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHH
QSGKPLLIIAEDVEGEALATLVVNKIRGTFNCVAVKAPGFGDRRKAMLEDIAILTGGQVI
HCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCHH
TEDLGLKLENADISMLGRAHKVTVTKEDTTIVEGAGDSKEIQNRIKQIRTQIENTDSDFD
HHHHCCEECCCCHHHHCCCEEEEEEECCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCC
REKLQERLAKLAGGVAVIKVGAATETELKEKKHRIEDALSATRAAVEEGLVAGGGTTLID
HHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
AIPALDELNLEGDEATGVDIVRKALEAPVRLIADNAGYEGSVIVEKVKSEDKGIGFDAYN
HHCCHHHCCCCCCCCCCHHHHHHHHHCCHHEEECCCCCCCHHHHHHHHCCCCCCCCCCCC
GEFVNMIESGIVDPAKVTRSALQNAASAAAMLLTTECLVADKEEDNDSNGNAGMPGGGMP
CHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
GGMGGMM
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA