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The map label for this gene is gyrB [H]

Identifier: 220930856

GI number: 220930856

Start: 5083

End: 7011

Strand: Direct

Name: gyrB [H]

Synonym: Hore_00060

Alternate gene names: 220930856

Gene position: 5083-7011 (Clockwise)

Preceding gene: 220930855

Following gene: 220930857

Centisome position: 0.2

GC content: 42.72

Gene sequence:

>1929_bases
ATGGCTGAAGAAAGTTTTTTAAATAACAAAGGTAATTATGAAGCAGAGCATATACAGGTTTTAGAGGGATTAGAAGCAGT
AAGAAAAAGGCCCGGGATGTATATTGGGTCTACAGGAATGGATGGTCTCCACCATCTTGTATATGAGGTTGTAGATAATA
GTATTGATGAGGCCATGCAGGGTTACTGTGACTTGATAGAAGTTATTATAAAACCCGGTAATATAATTACAGTTAAAGAT
AATGGCCGGGGAATCCCCGTAGACCCCCATCCCAAACTTAAAAAACCAGCCGTTGAGGTGGTTTTAACCGTTCTCCATGC
CGGAGGTAAGTTCGGAGGGGATGGTTATAAGGTTTCAGGTGGTCTTCACGGAGTTGGTGTATCTGTTGTCAATGCCCTGT
CCAAATGGCTGGAAGTTGAGGTCTGCTGGCGGGATGGTAAGGTATACCATCAAAAATATAACAGGGGTATCCCGGTATAT
GACCTGAAGGTTATTGGTGAATGTTCTGGAACAGGGACTACCATATCTTTTAAACCAGATGAGGAAATCTTCGAAGATGT
TAACTTTAAGTATAAGGTTCTGGCCCAGCGCTTAAAAGAACTTGCCTTCCTGAATAAAGGGGTCAGAATTATTTTAAGGG
ATGAACGGGAAGAGGAGGCCCGTCAGGATGAGTATTGTTTTGAAGGGGGATTAGTTTCTTTTGTTAAGTATCTGAATAAG
AATAAAAATCCCTTATTTGATGAACCTTTTTACCTTGAGGAAAAGGATGAAAATAGAGAAGTAGAGATTGCAATCCAGTA
CAATGATGGTTATGTTGATAGTATCTTTACTTTTGCCAATAATATAAATACCCGGGAAGGTGGAACCCATTTAAGTGGAT
TTAAGAGTGCCTTAACCAGAACTGTTAATGACTATGCCCGGAGTAAGGGTATTTTAAAAGAAAATGATGAAAATCTGACC
GGGGAGGATATCAGGGAAGGAATTACAGCTATTATCAGTGTCAGGCTTACAGATCCCCAGTTTGAGGGGCAGACCAAGAC
CAAACTGGGTAATAGTGAAATAAGGGGATTTGTTGATTCTGCCCTATCGAGTAATTTAAGGACCTTCCTTGAGGAAAACC
CTAAAATAGGGAAGATAATTGTGGAAAAGGCCAGGAGTGCCGCCCGGGCCCGGAAAGCTGCCAAAAAAGCCCGGGAGCTC
ACCAGGCGAAAAGGGGCCTTATCAAATACAGCTTTACCGGGTAAACTGGCTGATTGTTCTTCCCGTGATACTGAAAATAC
CGAAATTTATATAGTTGAGGGGGATTCAGCCGGTGGTTCTGCTAAACAGGGCCGGGACCGGAAGTTCCAGGCCATCTTAC
CGCTCCGGGGTAAGATTTTAAATGTAGAAAAGGCCAGATTGAATAAGATTCTCAACAATGAAGAAATAAGGGCCTTAATA
ACAGCCATCGGAACCGGAATTGGGGATGAATTTGATATAAATAAGGCCCGCTATGGCAAAATAATTATAATGACCGATGC
CGATGTTGATGGGGCCCATATCAGGACCCTGTTGCTTACCTTTTTCTACAGGTATATGCGTCCTTTAATTGAAAATGGCA
TGATATATATAGCCCAGCCTCCCCTCTATAAGGTGAAAAAGGGGAGAAGGGAAGAGTATGTTTATAACGACCGGGGTTTA
CAAAAACTACTCAATGACATAGGACGTGATAAAATAACCCTGCAGCGTTACAAAGGTCTTGGAGAGATGAACCCCGACCA
GCTCTGGGAAACTACCATGAACCCTGAAAACCGTATTATCCTCAAGGTCAAAATTGAGGATGCGGTAGTAGCAGATGAGA
CCTTTACTACCCTCATGGGGGATAAGGTCCAGCCCAGGCGCGAATTTATTCAGGAACATGCCCATGAAGTTCAGAACCTG
GATGTTTAA

Upstream 100 bases:

>100_bases
TTTGTTTTGACCGGTGAGACAGTCTACCTGTCTATGATTTCATCCCACACACTGGCTAAAAGGGTAAAAGAACTGTTTAA
AGAAAATGGGGGAAATTCCA

Downstream 100 bases:

>100_bases
TTTTATTAATTATTCATAGTTAATTTCCTTGTTAATTTCCTAAATATCCTTAACTAATTTATTTCCTTTTTATCTCTAAC
TTTACCAGCTTTATATAAAT

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 642; Mature: 641

Protein sequence:

>642_residues
MAEESFLNNKGNYEAEHIQVLEGLEAVRKRPGMYIGSTGMDGLHHLVYEVVDNSIDEAMQGYCDLIEVIIKPGNIITVKD
NGRGIPVDPHPKLKKPAVEVVLTVLHAGGKFGGDGYKVSGGLHGVGVSVVNALSKWLEVEVCWRDGKVYHQKYNRGIPVY
DLKVIGECSGTGTTISFKPDEEIFEDVNFKYKVLAQRLKELAFLNKGVRIILRDEREEEARQDEYCFEGGLVSFVKYLNK
NKNPLFDEPFYLEEKDENREVEIAIQYNDGYVDSIFTFANNINTREGGTHLSGFKSALTRTVNDYARSKGILKENDENLT
GEDIREGITAIISVRLTDPQFEGQTKTKLGNSEIRGFVDSALSSNLRTFLEENPKIGKIIVEKARSAARARKAAKKAREL
TRRKGALSNTALPGKLADCSSRDTENTEIYIVEGDSAGGSAKQGRDRKFQAILPLRGKILNVEKARLNKILNNEEIRALI
TAIGTGIGDEFDINKARYGKIIIMTDADVDGAHIRTLLLTFFYRYMRPLIENGMIYIAQPPLYKVKKGRREEYVYNDRGL
QKLLNDIGRDKITLQRYKGLGEMNPDQLWETTMNPENRIILKVKIEDAVVADETFTTLMGDKVQPRREFIQEHAHEVQNL
DV

Sequences:

>Translated_642_residues
MAEESFLNNKGNYEAEHIQVLEGLEAVRKRPGMYIGSTGMDGLHHLVYEVVDNSIDEAMQGYCDLIEVIIKPGNIITVKD
NGRGIPVDPHPKLKKPAVEVVLTVLHAGGKFGGDGYKVSGGLHGVGVSVVNALSKWLEVEVCWRDGKVYHQKYNRGIPVY
DLKVIGECSGTGTTISFKPDEEIFEDVNFKYKVLAQRLKELAFLNKGVRIILRDEREEEARQDEYCFEGGLVSFVKYLNK
NKNPLFDEPFYLEEKDENREVEIAIQYNDGYVDSIFTFANNINTREGGTHLSGFKSALTRTVNDYARSKGILKENDENLT
GEDIREGITAIISVRLTDPQFEGQTKTKLGNSEIRGFVDSALSSNLRTFLEENPKIGKIIVEKARSAARARKAAKKAREL
TRRKGALSNTALPGKLADCSSRDTENTEIYIVEGDSAGGSAKQGRDRKFQAILPLRGKILNVEKARLNKILNNEEIRALI
TAIGTGIGDEFDINKARYGKIIIMTDADVDGAHIRTLLLTFFYRYMRPLIENGMIYIAQPPLYKVKKGRREEYVYNDRGL
QKLLNDIGRDKITLQRYKGLGEMNPDQLWETTMNPENRIILKVKIEDAVVADETFTTLMGDKVQPRREFIQEHAHEVQNL
DV
>Mature_641_residues
AEESFLNNKGNYEAEHIQVLEGLEAVRKRPGMYIGSTGMDGLHHLVYEVVDNSIDEAMQGYCDLIEVIIKPGNIITVKDN
GRGIPVDPHPKLKKPAVEVVLTVLHAGGKFGGDGYKVSGGLHGVGVSVVNALSKWLEVEVCWRDGKVYHQKYNRGIPVYD
LKVIGECSGTGTTISFKPDEEIFEDVNFKYKVLAQRLKELAFLNKGVRIILRDEREEEARQDEYCFEGGLVSFVKYLNKN
KNPLFDEPFYLEEKDENREVEIAIQYNDGYVDSIFTFANNINTREGGTHLSGFKSALTRTVNDYARSKGILKENDENLTG
EDIREGITAIISVRLTDPQFEGQTKTKLGNSEIRGFVDSALSSNLRTFLEENPKIGKIIVEKARSAARARKAAKKARELT
RRKGALSNTALPGKLADCSSRDTENTEIYIVEGDSAGGSAKQGRDRKFQAILPLRGKILNVEKARLNKILNNEEIRALIT
AIGTGIGDEFDINKARYGKIIIMTDADVDGAHIRTLLLTFFYRYMRPLIENGMIYIAQPPLYKVKKGRREEYVYNDRGLQ
KLLNDIGRDKITLQRYKGLGEMNPDQLWETTMNPENRIILKVKIEDAVVADETFTTLMGDKVQPRREFIQEHAHEVQNLD
V

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=602, Percent_Identity=24.9169435215947, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI19913408, Length=602, Percent_Identity=25.0830564784053, Blast_Score=130, Evalue=5e-30,
Organism=Escherichia coli, GI48994957, Length=567, Percent_Identity=55.2028218694885, Blast_Score=602, Evalue=1e-173,
Organism=Escherichia coli, GI1789408, Length=633, Percent_Identity=40.2843601895735, Blast_Score=447, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI17535065, Length=607, Percent_Identity=25.0411861614498, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI212645845, Length=602, Percent_Identity=24.7508305647841, Blast_Score=134, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI212645657, Length=177, Percent_Identity=28.2485875706215, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324241, Length=620, Percent_Identity=25.9677419354839, Blast_Score=113, Evalue=9e-26,
Organism=Drosophila melanogaster, GI17136538, Length=667, Percent_Identity=25.3373313343328, Blast_Score=148, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 72304; Mature: 72173

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEESFLNNKGNYEAEHIQVLEGLEAVRKRPGMYIGSTGMDGLHHLVYEVVDNSIDEAMQ
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHH
GYCDLIEVIIKPGNIITVKDNGRGIPVDPHPKLKKPAVEVVLTVLHAGGKFGGDGYKVSG
HHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEECC
GLHGVGVSVVNALSKWLEVEVCWRDGKVYHQKYNRGIPVYDLKVIGECSGTGTTISFKPD
CCCHHHHHHHHHHHHHHEEEEEECCCHHHHHHHCCCCCEEEEEEEEECCCCCCEEEECCC
EEIFEDVNFKYKVLAQRLKELAFLNKGVRIILRDEREEEARQDEYCFEGGLVSFVKYLNK
HHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCCHHHHHHHHCC
NKNPLFDEPFYLEEKDENREVEIAIQYNDGYVDSIFTFANNINTREGGTHLSGFKSALTR
CCCCCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
TVNDYARSKGILKENDENLTGEDIREGITAIISVRLTDPQFEGQTKTKLGNSEIRGFVDS
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHEEEEEEECCCCCCCCHHHCCCCHHHHHHHHH
ALSSNLRTFLEENPKIGKIIVEKARSAARARKAAKKARELTRRKGALSNTALPGKLADCS
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
SRDTENTEIYIVEGDSAGGSAKQGRDRKFQAILPLRGKILNVEKARLNKILNNEEIRALI
CCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCEEECHHHHHHHHHHCCHHHHHHH
TAIGTGIGDEFDINKARYGKIIIMTDADVDGAHIRTLLLTFFYRYMRPLIENGMIYIAQP
HHHHCCCCCCCCCCCHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECC
PLYKVKKGRREEYVYNDRGLQKLLNDIGRDKITLQRYKGLGEMNPDQLWETTMNPENRII
CCHHHHCCCCHHHEECHHHHHHHHHHCCCCCEEHHHHCCCCCCCHHHHHHHCCCCCCEEE
LKVKIEDAVVADETFTTLMGDKVQPRREFIQEHAHEVQNLDV
EEEEECCEEECCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AEESFLNNKGNYEAEHIQVLEGLEAVRKRPGMYIGSTGMDGLHHLVYEVVDNSIDEAMQ
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHH
GYCDLIEVIIKPGNIITVKDNGRGIPVDPHPKLKKPAVEVVLTVLHAGGKFGGDGYKVSG
HHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEECC
GLHGVGVSVVNALSKWLEVEVCWRDGKVYHQKYNRGIPVYDLKVIGECSGTGTTISFKPD
CCCHHHHHHHHHHHHHHEEEEEECCCHHHHHHHCCCCCEEEEEEEEECCCCCCEEEECCC
EEIFEDVNFKYKVLAQRLKELAFLNKGVRIILRDEREEEARQDEYCFEGGLVSFVKYLNK
HHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCCHHHHHHHHCC
NKNPLFDEPFYLEEKDENREVEIAIQYNDGYVDSIFTFANNINTREGGTHLSGFKSALTR
CCCCCCCCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
TVNDYARSKGILKENDENLTGEDIREGITAIISVRLTDPQFEGQTKTKLGNSEIRGFVDS
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHEEEEEEECCCCCCCCHHHCCCCHHHHHHHHH
ALSSNLRTFLEENPKIGKIIVEKARSAARARKAAKKARELTRRKGALSNTALPGKLADCS
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
SRDTENTEIYIVEGDSAGGSAKQGRDRKFQAILPLRGKILNVEKARLNKILNNEEIRALI
CCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCEEECHHHHHHHHHHCCHHHHHHH
TAIGTGIGDEFDINKARYGKIIIMTDADVDGAHIRTLLLTFFYRYMRPLIENGMIYIAQP
HHHHCCCCCCCCCCCHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECC
PLYKVKKGRREEYVYNDRGLQKLLNDIGRDKITLQRYKGLGEMNPDQLWETTMNPENRII
CCHHHHCCCCHHHEECHHHHHHHHHHCCCCCEEHHHHCCCCCCCHHHHHHHCCCCCCEEE
LKVKIEDAVVADETFTTLMGDKVQPRREFIQEHAHEVQNLDV
EEEEECCEEECCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]