Click here to switch to the map view.

The map label for this gene is fcl [C]

Identifier: 220919526

GI number: 220919526

Start: 4950607

End: 4951557

Strand: Direct

Name: fcl [C]

Synonym: A2cp1_4447

Alternate gene names: 220919526

Gene position: 4950607-4951557 (Clockwise)

Preceding gene: 220919525

Following gene: 220919527

Centisome position: 98.43

GC content: 69.09

Gene sequence:

>951_bases
ATGAAATTCGACTGGTCCTCCGAGCGCGTGGTCGTCACCGGCGGCGCGGGGTTCCTCGGCGGCTTCGTCCAGGAAGCGCT
GCGGCGGCGCGGCGCGAAGGACGTCTTCGTGCCCCGCTCGAAGGACTACGACCTCGTCCAGATGGAGGGGGTCCGCGCGC
TCTACCGCGACGCCCGGCCGACGATGGTCCTGCACCTCGCCGCGCGGGTGGGCGGCATCGGGGCGAACCGCGACAACCCG
GGCAAGTTCTTCTACGACAACCTCATGATGGGCGTTCAGCTCATCGAGGTGGGCCGCCAGGTCGGCCTCAAGAAGCTCGT
CGCGCTCGGCACCATCTGCGCGTACCCGAAGTTCTGCCCGGTGCCGTTCAAGGAAGAAGACCTCTGGAACGGCTATCCGG
AGGAGACGAACGCGCCCTACGGCCTCGCCAAGAAGATGCTGCTCGTGCAGAGCGAGGCGTACCGGCAGCAGTACGGGTTC
CACTCGGTGGTCCTGTTCCCGGTGAACCTGTACGGCCCGCACGACAACTTCGACCTGCGCACCTCGCACGTCATCCCGGC
GCTCGTCCGGAAGTGCGTCGAGGCGCGCGAGCGCGGCGACAAGCAGATCGTCGTGTGGGGCGACGGCTCGGCCTCGCGCG
AGTTCCTGCACGCGCGCGACGCGGCCGAGGGCATCCTCGACGCGGCCGAGCGCTACGACAGGTCCGAGGCGGTGAACCTG
GGCGCGGGGTTCGAGATCAAGATCCGCGACCTCGTGCCGCTCGTGGCCCGGCTGTGCCGCTTCGAGGGCGAGCTCGTCTG
GGACACCACCAAGCCGAACGGCCAGCCGCGGCGCATGCTCGACACGTCGAAGGCCCTGCGCGAGTTCGGCTGGAAGGCGC
GCATCGGGTTCGAGGACGGGCTGCGCGAGACGGTGGAGTGGTTCGAGGCCAACCGAGGGAGCATCCCGTGA

Upstream 100 bases:

>100_bases
GCCGGCCCGGCCGCAGGTCCCCGGCGCCGGCTGGCGGCGAAGCGCGGGCGGCGGGTGCCGCAATTGACGCGAGCCGCCCC
GCCCCCTACGCTCCGCCCCC

Downstream 100 bases:

>100_bases
AGAAGGCGTTCATCACCGGCATCACCGGCCAGGACGGCAGCTACCTCGCGGAGCTGCTGCTCGAGAAGGGCTACGAGGTC
CACGGCATGGTCCGCCGCTC

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MKFDWSSERVVVTGGAGFLGGFVQEALRRRGAKDVFVPRSKDYDLVQMEGVRALYRDARPTMVLHLAARVGGIGANRDNP
GKFFYDNLMMGVQLIEVGRQVGLKKLVALGTICAYPKFCPVPFKEEDLWNGYPEETNAPYGLAKKMLLVQSEAYRQQYGF
HSVVLFPVNLYGPHDNFDLRTSHVIPALVRKCVEARERGDKQIVVWGDGSASREFLHARDAAEGILDAAERYDRSEAVNL
GAGFEIKIRDLVPLVARLCRFEGELVWDTTKPNGQPRRMLDTSKALREFGWKARIGFEDGLRETVEWFEANRGSIP

Sequences:

>Translated_316_residues
MKFDWSSERVVVTGGAGFLGGFVQEALRRRGAKDVFVPRSKDYDLVQMEGVRALYRDARPTMVLHLAARVGGIGANRDNP
GKFFYDNLMMGVQLIEVGRQVGLKKLVALGTICAYPKFCPVPFKEEDLWNGYPEETNAPYGLAKKMLLVQSEAYRQQYGF
HSVVLFPVNLYGPHDNFDLRTSHVIPALVRKCVEARERGDKQIVVWGDGSASREFLHARDAAEGILDAAERYDRSEAVNL
GAGFEIKIRDLVPLVARLCRFEGELVWDTTKPNGQPRRMLDTSKALREFGWKARIGFEDGLRETVEWFEANRGSIP
>Mature_316_residues
MKFDWSSERVVVTGGAGFLGGFVQEALRRRGAKDVFVPRSKDYDLVQMEGVRALYRDARPTMVLHLAARVGGIGANRDNP
GKFFYDNLMMGVQLIEVGRQVGLKKLVALGTICAYPKFCPVPFKEEDLWNGYPEETNAPYGLAKKMLLVQSEAYRQQYGF
HSVVLFPVNLYGPHDNFDLRTSHVIPALVRKCVEARERGDKQIVVWGDGSASREFLHARDAAEGILDAAERYDRSEAVNL
GAGFEIKIRDLVPLVARLCRFEGELVWDTTKPNGQPRRMLDTSKALREFGWKARIGFEDGLRETVEWFEANRGSIP

Specific function: Putative nucleotide sugar epimerase/dehydrogenase [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=312, Percent_Identity=34.9358974358974, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI7657641, Length=334, Percent_Identity=22.1556886227545, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI42516563, Length=331, Percent_Identity=23.5649546827795, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1788365, Length=316, Percent_Identity=35.7594936708861, Blast_Score=217, Evalue=8e-58,
Organism=Escherichia coli, GI48994969, Length=344, Percent_Identity=21.8023255813953, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1788353, Length=348, Percent_Identity=23.5632183908046, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1786974, Length=337, Percent_Identity=21.6617210682493, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17554428, Length=310, Percent_Identity=31.2903225806452, Blast_Score=176, Evalue=1e-44,
Organism=Drosophila melanogaster, GI19922778, Length=314, Percent_Identity=31.2101910828025, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI21356223, Length=319, Percent_Identity=23.5109717868339, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46

Molecular weight: Translated: 35617; Mature: 35617

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFDWSSERVVVTGGAGFLGGFVQEALRRRGAKDVFVPRSKDYDLVQMEGVRALYRDARP
CCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHHHHCCCC
TMVLHLAARVGGIGANRDNPGKFFYDNLMMGVQLIEVGRQVGLKKLVALGTICAYPKFCP
HHEEHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VPFKEEDLWNGYPEETNAPYGLAKKMLLVQSEAYRQQYGFHSVVLFPVNLYGPHDNFDLR
CCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCC
TSHVIPALVRKCVEARERGDKQIVVWGDGSASREFLHARDAAEGILDAAERYDRSEAVNL
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHEEC
GAGFEIKIRDLVPLVARLCRFEGELVWDTTKPNGQPRRMLDTSKALREFGWKARIGFEDG
CCCCEEEHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCEEEECHHHH
LRETVEWFEANRGSIP
HHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKFDWSSERVVVTGGAGFLGGFVQEALRRRGAKDVFVPRSKDYDLVQMEGVRALYRDARP
CCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHHHHCCCC
TMVLHLAARVGGIGANRDNPGKFFYDNLMMGVQLIEVGRQVGLKKLVALGTICAYPKFCP
HHEEHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VPFKEEDLWNGYPEETNAPYGLAKKMLLVQSEAYRQQYGFHSVVLFPVNLYGPHDNFDLR
CCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCC
TSHVIPALVRKCVEARERGDKQIVVWGDGSASREFLHARDAAEGILDAAERYDRSEAVNL
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHEEC
GAGFEIKIRDLVPLVARLCRFEGELVWDTTKPNGQPRRMLDTSKALREFGWKARIGFEDG
CCCCEEEHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCEEEECHHHH
LRETVEWFEANRGSIP
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDPglucose

Specific reaction: dTDP-glucose = dTDP-4-dehydro-6-deoxy-D-glucose + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]