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The map label for this gene is ddl

Identifier: 220918999

GI number: 220918999

Start: 4354770

End: 4355696

Strand: Direct

Name: ddl

Synonym: A2cp1_3914

Alternate gene names: 220918999

Gene position: 4354770-4355696 (Clockwise)

Preceding gene: 220918998

Following gene: 220919001

Centisome position: 86.59

GC content: 72.71

Gene sequence:

>927_bases
ATGAGCACGTGGACGGGCAAGCGGGTGGCGGTGCTGTACGGCGGACGCTCCAGCGAGCGCGAGGTGTCGCTCAGGACCGG
CGCCGCCTGCGCCGACGCGCTCCGGCAGAAGGGGCACGAGGTGGTGCTGCTGGACGTGGACCTCGAGGTGGCGGCGCGGC
TCCGCGCCGAGCGGGTCGAGGTCGCGTTCGTGGCGCTGCACGGCCGCTTCGGCGAGGACGGCAGCATCCAGGGGCTGCTC
GAGTCGATGGCCATCCCGTACACCGGCTCCGGCGTGCTCGCCTCGGCCATGGGCATGGACAAGACCGTGTCGAAGGCGAT
CTTCCGCTCGCTCGGCCTGGCCGTCGCCGACTACCGCGTGTTCCCGCGCGCCTCGGCCGGCTCGATCGGCGTGGGCGATC
TCCCGTTCGGCCTTCCGTGCGTGGTGAAGCCCGCCGGCGAGGGCTCGTCCGTGGGCGTGCACCTCGTGAACGAGGCCGCG
GAGCTCGGCCCCGCCTGCCGCGACGCCGCCTCCCACGCCGGCGACGTGATCGTCGAGCGGTACGTCAAGGGCACCGAGGT
GGACGTGGCGGTGCTGGACGGGAAGGCGCTCGGGGCGATCGAGATCGTCCCCGCGAACGCGTTCTACGACTACGCCGCGA
AGTACACCGCCGGCACCACGAAGTACTTCTACCCGGCGCGCCTCCCCGAGGCGCACGTCCGCGCGGTGATGCAGGCGGCC
GAGGCCGCGCACCGCGGGATCGGCTGCAGCGGCGTGACCCGCGTGGACTTCATCGTGGCCGGCGACGGGACGCCGTACAT
CCTGGAGGTGAACACGCTGCCCGGGATGACCGCGACGTCGCTCGTCCCGAAGATCGCCGCCGGCCTGGGCCTGTCGTTCC
CCGACCTGTGCGAGCGGATCCTGGACGGCGCTGCGTTGAAGGCCTGA

Upstream 100 bases:

>100_bases
GCGAGTTCCTCGAAGAGGATCTGGAAGGGCTGGACGGGCACGCCGCCGCGGGCGGCGGCCCCGGCGCGGCGAGCGGGGGC
GTGCGCCCCCCGGAGGCGAC

Downstream 100 bases:

>100_bases
GCGAGGTCCGCGCCGCCCGTCAGCGGGCGACGTACGCCTTCGCCTCGTGGCACGCCTTCGCGCAGCTCCCGCCGCTGGCG
GTGCGCGTGAAGCCGAGCTT

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MSTWTGKRVAVLYGGRSSEREVSLRTGAACADALRQKGHEVVLLDVDLEVAARLRAERVEVAFVALHGRFGEDGSIQGLL
ESMAIPYTGSGVLASAMGMDKTVSKAIFRSLGLAVADYRVFPRASAGSIGVGDLPFGLPCVVKPAGEGSSVGVHLVNEAA
ELGPACRDAASHAGDVIVERYVKGTEVDVAVLDGKALGAIEIVPANAFYDYAAKYTAGTTKYFYPARLPEAHVRAVMQAA
EAAHRGIGCSGVTRVDFIVAGDGTPYILEVNTLPGMTATSLVPKIAAGLGLSFPDLCERILDGAALKA

Sequences:

>Translated_308_residues
MSTWTGKRVAVLYGGRSSEREVSLRTGAACADALRQKGHEVVLLDVDLEVAARLRAERVEVAFVALHGRFGEDGSIQGLL
ESMAIPYTGSGVLASAMGMDKTVSKAIFRSLGLAVADYRVFPRASAGSIGVGDLPFGLPCVVKPAGEGSSVGVHLVNEAA
ELGPACRDAASHAGDVIVERYVKGTEVDVAVLDGKALGAIEIVPANAFYDYAAKYTAGTTKYFYPARLPEAHVRAVMQAA
EAAHRGIGCSGVTRVDFIVAGDGTPYILEVNTLPGMTATSLVPKIAAGLGLSFPDLCERILDGAALKA
>Mature_307_residues
STWTGKRVAVLYGGRSSEREVSLRTGAACADALRQKGHEVVLLDVDLEVAARLRAERVEVAFVALHGRFGEDGSIQGLLE
SMAIPYTGSGVLASAMGMDKTVSKAIFRSLGLAVADYRVFPRASAGSIGVGDLPFGLPCVVKPAGEGSSVGVHLVNEAAE
LGPACRDAASHAGDVIVERYVKGTEVDVAVLDGKALGAIEIVPANAFYDYAAKYTAGTTKYFYPARLPEAHVRAVMQAAE
AAHRGIGCSGVTRVDFIVAGDGTPYILEVNTLPGMTATSLVPKIAAGLGLSFPDLCERILDGAALKA

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=302, Percent_Identity=44.3708609271523, Blast_Score=226, Evalue=1e-60,
Organism=Escherichia coli, GI1786579, Length=349, Percent_Identity=34.3839541547278, Blast_Score=167, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_ANAD2 (B8J8F1)

Other databases:

- EMBL:   CP001359
- RefSeq:   YP_002494303.1
- ProteinModelPortal:   B8J8F1
- SMR:   B8J8F1
- GeneID:   7297254
- GenomeReviews:   CP001359_GR
- KEGG:   acp:A2cp1_3914
- HOGENOM:   HBG427092
- ProtClustDB:   PRK01372
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 32036; Mature: 31905

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTWTGKRVAVLYGGRSSEREVSLRTGAACADALRQKGHEVVLLDVDLEVAARLRAERVE
CCCCCCCEEEEEECCCCCCCEEEECCCHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHEE
VAFVALHGRFGEDGSIQGLLESMAIPYTGSGVLASAMGMDKTVSKAIFRSLGLAVADYRV
EEEEEEECCCCCCCCHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHHCCHHHHEEE
FPRASAGSIGVGDLPFGLPCVVKPAGEGSSVGVHLVNEAAELGPACRDAASHAGDVIVER
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEHHHHHHCHHHHHHHHHHHHHHHHH
YVKGTEVDVAVLDGKALGAIEIVPANAFYDYAAKYTAGTTKYFYPARLPEAHVRAVMQAA
HCCCCCEEEEEECCCCCCEEEEEECCHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHH
EAAHRGIGCSGVTRVDFIVAGDGTPYILEVNTLPGMTATSLVPKIAAGLGLSFPDLCERI
HHHHCCCCCCCCEEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHH
LDGAALKA
HCCCCCCC
>Mature Secondary Structure 
STWTGKRVAVLYGGRSSEREVSLRTGAACADALRQKGHEVVLLDVDLEVAARLRAERVE
CCCCCCEEEEEECCCCCCCEEEECCCHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHEE
VAFVALHGRFGEDGSIQGLLESMAIPYTGSGVLASAMGMDKTVSKAIFRSLGLAVADYRV
EEEEEEECCCCCCCCHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHHCCHHHHEEE
FPRASAGSIGVGDLPFGLPCVVKPAGEGSSVGVHLVNEAAELGPACRDAASHAGDVIVER
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEHHHHHHCHHHHHHHHHHHHHHHHH
YVKGTEVDVAVLDGKALGAIEIVPANAFYDYAAKYTAGTTKYFYPARLPEAHVRAVMQAA
HCCCCCEEEEEECCCCCCEEEEEECCHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHH
EAAHRGIGCSGVTRVDFIVAGDGTPYILEVNTLPGMTATSLVPKIAAGLGLSFPDLCERI
HHHHCCCCCCCCEEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHH
LDGAALKA
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA