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The map label for this gene is bioB

Identifier: 220918695

GI number: 220918695

Start: 4023848

End: 4024933

Strand: Reverse

Name: bioB

Synonym: A2cp1_3603

Alternate gene names: 220918695

Gene position: 4024933-4023848 (Counterclockwise)

Preceding gene: 220918697

Following gene: 220918694

Centisome position: 80.03

GC content: 73.76

Gene sequence:

>1086_bases
ATGTGCGAGACCGCTTCCAGGACGCTGCCGCCCGGCATCACGCCGATCTCCGGTGACGAGGCCCGCCGCCTCATCCACCA
CACGTCAGGCCCCGAGCTCGAGGCGCTGCTCGACCGGGCCGAGGCGGTCCGGCGCGCGGTGCACGGCGACGAGGTCGCGC
TGTGCGGCATCACGAACGCGAAGAGCGGCCGCTGCCCCGAGGACTGCGGCTTCTGCTCGCAGTCGGCGCGCTTCGAGGGC
GCCGACGCGCCCGTGTACCCGATGATCGGCGCCGGCGAGATCGTGGAGCAGGCGCACAAGGCGGAGCGGGCCGGCGCGCG
CGAGTTCTCGATCGTGGCGAGCGGGACGCGCCTGGCGCGGGAGCAGGAGCTCGCGACCGTCGAGGAGGCGCTGCGGCGGC
TGCGGGCGGAGACCGCGGTGGAGCCCTGCGCCTCGCTCGGCCTCATGCGCGAGCCGGAGCTGCGGCGGCTGAAGGACGCC
GGGCTCATGCACTACCACCACAACCTCGAGACCGCGCGCAGCCACTTCGAGAACGTCTGCACGACGCACACGTTCGACGA
GCAGCTCGAGACCATCCGCGCCGCGAAGGGGCTCGGGCTGAAGCTCTGCTCCGGCGGCATCCTGGGCATGGGCGAGACCC
CGGAGCAGCGCGTGGAGTTCGCCGAGGAGGTCCGCGACCTCGGCGTGGACTGCGTGCCGGTGAACTTCCTCAACCCGCGC
GCCGGCACGCCCATGGCGCACCTGAAGGCCATCACGCCGGAGGAGTGCCTGGCGGCCGTGGCCGTGTTCCGCCTGATGAT
GCCGGCGGCGCACATCTTCGTGATGGGCGGGCGCGAGGTGAACCTCGGCGACCGGCAGGACCTCATCTTCCGCGCCGGCG
CGAACGGCACCATGGTCGGCAACTACCTCACCAGCGCCGGCCGCGCGCCCGACCTCACCGTCGGCATGGTGGAGCGGCAG
GGCCTCACGCTCCGGCCGCCCGACACCGGGAAGGCCTGGGCGTTCGACGGCCACGCGCCGTCCGACGCCGACTGGAACCG
CAAGGCCGCGGAGCCGCGCCCGCGGCCGCTGCCGGTGGTCCGCTAG

Upstream 100 bases:

>100_bases
CGGTCCGGAGGAGGCGCCCCCTGCCTGGTTGGGGGTCGTCAACCCACGTGCGACCGCCTGGTTGACGAGCGGATCCCCGC
CTGTCTATCGTGCGGGCCCC

Downstream 100 bases:

>100_bases
GCGATGGCGCGCGGCGCGCTCGACTGGATCGGCGCCGAGCTCGAGGCCCTCGACGCGAAGGGCCTGCGCCGGTCCCTCGA
GCCCATCGGCCCGGCGCAGG

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MCETASRTLPPGITPISGDEARRLIHHTSGPELEALLDRAEAVRRAVHGDEVALCGITNAKSGRCPEDCGFCSQSARFEG
ADAPVYPMIGAGEIVEQAHKAERAGAREFSIVASGTRLAREQELATVEEALRRLRAETAVEPCASLGLMREPELRRLKDA
GLMHYHHNLETARSHFENVCTTHTFDEQLETIRAAKGLGLKLCSGGILGMGETPEQRVEFAEEVRDLGVDCVPVNFLNPR
AGTPMAHLKAITPEECLAAVAVFRLMMPAAHIFVMGGREVNLGDRQDLIFRAGANGTMVGNYLTSAGRAPDLTVGMVERQ
GLTLRPPDTGKAWAFDGHAPSDADWNRKAAEPRPRPLPVVR

Sequences:

>Translated_361_residues
MCETASRTLPPGITPISGDEARRLIHHTSGPELEALLDRAEAVRRAVHGDEVALCGITNAKSGRCPEDCGFCSQSARFEG
ADAPVYPMIGAGEIVEQAHKAERAGAREFSIVASGTRLAREQELATVEEALRRLRAETAVEPCASLGLMREPELRRLKDA
GLMHYHHNLETARSHFENVCTTHTFDEQLETIRAAKGLGLKLCSGGILGMGETPEQRVEFAEEVRDLGVDCVPVNFLNPR
AGTPMAHLKAITPEECLAAVAVFRLMMPAAHIFVMGGREVNLGDRQDLIFRAGANGTMVGNYLTSAGRAPDLTVGMVERQ
GLTLRPPDTGKAWAFDGHAPSDADWNRKAAEPRPRPLPVVR
>Mature_361_residues
MCETASRTLPPGITPISGDEARRLIHHTSGPELEALLDRAEAVRRAVHGDEVALCGITNAKSGRCPEDCGFCSQSARFEG
ADAPVYPMIGAGEIVEQAHKAERAGAREFSIVASGTRLAREQELATVEEALRRLRAETAVEPCASLGLMREPELRRLKDA
GLMHYHHNLETARSHFENVCTTHTFDEQLETIRAAKGLGLKLCSGGILGMGETPEQRVEFAEEVRDLGVDCVPVNFLNPR
AGTPMAHLKAITPEECLAAVAVFRLMMPAAHIFVMGGREVNLGDRQDLIFRAGANGTMVGNYLTSAGRAPDLTVGMVERQ
GLTLRPPDTGKAWAFDGHAPSDADWNRKAAEPRPRPLPVVR

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=297, Percent_Identity=31.3131313131313, Blast_Score=159, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6321725, Length=310, Percent_Identity=33.2258064516129, Blast_Score=166, Evalue=4e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_ANAD2 (B8J638)

Other databases:

- EMBL:   CP001359
- RefSeq:   YP_002493999.1
- ProteinModelPortal:   B8J638
- SMR:   B8J638
- GeneID:   7298725
- GenomeReviews:   CP001359_GR
- KEGG:   acp:A2cp1_3603
- HOGENOM:   HBG405569
- ProtClustDB:   CLSK946684
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 39124; Mature: 39124

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCETASRTLPPGITPISGDEARRLIHHTSGPELEALLDRAEAVRRAVHGDEVALCGITNA
CCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCC
KSGRCPEDCGFCSQSARFEGADAPVYPMIGAGEIVEQAHKAERAGAREFSIVASGTRLAR
CCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHH
EQELATVEEALRRLRAETAVEPCASLGLMREPELRRLKDAGLMHYHHNLETARSHFENVC
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
TTHTFDEQLETIRAAKGLGLKLCSGGILGMGETPEQRVEFAEEVRDLGVDCVPVNFLNPR
HCCCHHHHHHHHHHHCCCCEEEECCCEECCCCCHHHHHHHHHHHHHCCCCEEEECCCCCC
AGTPMAHLKAITPEECLAAVAVFRLMMPAAHIFVMGGREVNLGDRQDLIFRAGANGTMVG
CCCCHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEECCCEECCCCCCCEEEECCCCCCEEH
NYLTSAGRAPDLTVGMVERQGLTLRPPDTGKAWAFDGHAPSDADWNRKAAEPRPRPLPVV
HHHHCCCCCCCCEEEEECCCCCEECCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCC
R
C
>Mature Secondary Structure
MCETASRTLPPGITPISGDEARRLIHHTSGPELEALLDRAEAVRRAVHGDEVALCGITNA
CCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCC
KSGRCPEDCGFCSQSARFEGADAPVYPMIGAGEIVEQAHKAERAGAREFSIVASGTRLAR
CCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHH
EQELATVEEALRRLRAETAVEPCASLGLMREPELRRLKDAGLMHYHHNLETARSHFENVC
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
TTHTFDEQLETIRAAKGLGLKLCSGGILGMGETPEQRVEFAEEVRDLGVDCVPVNFLNPR
HCCCHHHHHHHHHHHCCCCEEEECCCEECCCCCHHHHHHHHHHHHHCCCCEEEECCCCCC
AGTPMAHLKAITPEECLAAVAVFRLMMPAAHIFVMGGREVNLGDRQDLIFRAGANGTMVG
CCCCHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEECCCEECCCCCCCEEEECCCCCCEEH
NYLTSAGRAPDLTVGMVERQGLTLRPPDTGKAWAFDGHAPSDADWNRKAAEPRPRPLPVV
HHHHCCCCCCCCEEEEECCCCCEECCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA