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The map label for this gene is bioF

Identifier: 220918694

GI number: 220918694

Start: 4022648

End: 4023844

Strand: Reverse

Name: bioF

Synonym: A2cp1_3602

Alternate gene names: 220918694

Gene position: 4023844-4022648 (Counterclockwise)

Preceding gene: 220918695

Following gene: 220918683

Centisome position: 80.01

GC content: 78.61

Gene sequence:

>1197_bases
ATGGCGCGCGGCGCGCTCGACTGGATCGGCGCCGAGCTCGAGGCCCTCGACGCGAAGGGCCTGCGCCGGTCCCTCGAGCC
CATCGGCCCGGCGCAGGGGCCGGTGGTGCAGGTGGGCGGCCGCGCGCTCGTGAACCTCTGCTCCAACGACTACCTCGGGC
TGGCCGCGGATCCGCGCGTCCGCGCGGCGGCCGCCGACGCCGCCATGCGCTTCGGCGCGGGCAGCGGCGCGGCGCGGCTC
GTCGCGGGCGACCTGCCGCCACACGGCGCCCTGGAGGCGCGGCTCGCCGCGTGGAAGGGCCGGGAGGCCGCGCTGCTGTT
CGGCTCGGGCTACCACGCGAACGCCGGCGTGCCCGGCGCGCTGGTGGGGCGCGACGACGCGGTGTTCTCCGACGTCCTGA
ACCACGCCTCGATCGTGGACGGCTGCCTGCTGTCGCGCGCCGAGCTGGTCCGCTACCGCCACTGCGACGTGGAGGAGCTG
GCCGGGCTGCTGGCGCGCACGCGGGCCCGCCGGAAGCTGGTGGTGACCGACGCGATCTTCTCCATGGACGGCGACGCCGC
GCCGCTCCGCGAGCTGGCCGAGCTGTGCGACCGCCACGGGGCCATGCTCTACGTGGACGAGGCGCACGCGGCCGGCGTGC
TCGGGCCGAACGGCGCCGGCCTGGCCGAGGCGCTCGGGGTGCAGGATCGCGTGGACGTGCACATGGGCACGCTGGGCAAG
GCGCTCGGCGCGTTCGGCGCGTACGTCGCCGGCGAGCGGCGCCTCATCGAGCTGCTCGTCTCCCGGGCGCGGCCGTTCGT
CTTCAGCACCGCGCTCCCGCCGCCCGCCTGCGCCGCGGCGCTCGCCGCGCTGGAGGTGGTGGCGACCGAGCCGTCGCGGC
GGACCCACCTGTTCGCGCTCTGCGCGCGGATGCAGGCCGGGCTGGCGCGGCTCGGGTTCGACGTGGCGCGGGTCGCGTCG
CCCATCTTCCCGGTGGTGCTGGGCACCGAGGCGCGGGCGCTGGCGGCGGCGGCGGCGCTCCGCGAGCGGGGCTGGTTCGT
GCGCGCCATCCGCCCGCCCACCGTGCCGCACGGCACGAGCCGGCTGCGGGTGGCGCTCTCGGCCGCGCACGACGCGGCGC
AGGTGGACGGGTTCCTGGCGGCGCTGGCGGCGGTGCTGCCGGACCTGCCGCCGGCCGGGCCGCGGCGGGCCGGCTAG

Upstream 100 bases:

>100_bases
GAAGGCCTGGGCGTTCGACGGCCACGCGCCGTCCGACGCCGACTGGAACCGCAAGGCCGCGGAGCCGCGCCCGCGGCCGC
TGCCGGTGGTCCGCTAGGCG

Downstream 100 bases:

>100_bases
CCGGAGCTCAACGCCGTCCCGGCTCGCGACCCGACGGGCGCGCCGCCGGCGGGCGCGCACCCCTCCGCGTTCAGCGCGCG
TGGCCGAGCATGTACCACTG

Product: 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MARGALDWIGAELEALDAKGLRRSLEPIGPAQGPVVQVGGRALVNLCSNDYLGLAADPRVRAAAADAAMRFGAGSGAARL
VAGDLPPHGALEARLAAWKGREAALLFGSGYHANAGVPGALVGRDDAVFSDVLNHASIVDGCLLSRAELVRYRHCDVEEL
AGLLARTRARRKLVVTDAIFSMDGDAAPLRELAELCDRHGAMLYVDEAHAAGVLGPNGAGLAEALGVQDRVDVHMGTLGK
ALGAFGAYVAGERRLIELLVSRARPFVFSTALPPPACAAALAALEVVATEPSRRTHLFALCARMQAGLARLGFDVARVAS
PIFPVVLGTEARALAAAAALRERGWFVRAIRPPTVPHGTSRLRVALSAAHDAAQVDGFLAALAAVLPDLPPAGPRRAG

Sequences:

>Translated_398_residues
MARGALDWIGAELEALDAKGLRRSLEPIGPAQGPVVQVGGRALVNLCSNDYLGLAADPRVRAAAADAAMRFGAGSGAARL
VAGDLPPHGALEARLAAWKGREAALLFGSGYHANAGVPGALVGRDDAVFSDVLNHASIVDGCLLSRAELVRYRHCDVEEL
AGLLARTRARRKLVVTDAIFSMDGDAAPLRELAELCDRHGAMLYVDEAHAAGVLGPNGAGLAEALGVQDRVDVHMGTLGK
ALGAFGAYVAGERRLIELLVSRARPFVFSTALPPPACAAALAALEVVATEPSRRTHLFALCARMQAGLARLGFDVARVAS
PIFPVVLGTEARALAAAAALRERGWFVRAIRPPTVPHGTSRLRVALSAAHDAAQVDGFLAALAAVLPDLPPAGPRRAG
>Mature_397_residues
ARGALDWIGAELEALDAKGLRRSLEPIGPAQGPVVQVGGRALVNLCSNDYLGLAADPRVRAAAADAAMRFGAGSGAARLV
AGDLPPHGALEARLAAWKGREAALLFGSGYHANAGVPGALVGRDDAVFSDVLNHASIVDGCLLSRAELVRYRHCDVEELA
GLLARTRARRKLVVTDAIFSMDGDAAPLRELAELCDRHGAMLYVDEAHAAGVLGPNGAGLAEALGVQDRVDVHMGTLGKA
LGAFGAYVAGERRLIELLVSRARPFVFSTALPPPACAAALAALEVVATEPSRRTHLFALCARMQAGLARLGFDVARVASP
IFPVVLGTEARALAAAAALRERGWFVRAIRPPTVPHGTSRLRVALSAAHDAAQVDGFLAALAAVLPDLPPAGPRRAG

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily

Homologues:

Organism=Homo sapiens, GI7657118, Length=387, Percent_Identity=41.343669250646, Blast_Score=270, Evalue=2e-72,
Organism=Homo sapiens, GI284448556, Length=413, Percent_Identity=38.7409200968523, Blast_Score=259, Evalue=2e-69,
Organism=Homo sapiens, GI83977442, Length=349, Percent_Identity=35.243553008596, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI83977440, Length=349, Percent_Identity=35.243553008596, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI83977444, Length=349, Percent_Identity=35.243553008596, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI4502025, Length=349, Percent_Identity=36.3896848137536, Blast_Score=186, Evalue=5e-47,
Organism=Homo sapiens, GI40316939, Length=349, Percent_Identity=36.3896848137536, Blast_Score=186, Evalue=5e-47,
Organism=Homo sapiens, GI4758668, Length=366, Percent_Identity=32.7868852459016, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI119220554, Length=362, Percent_Identity=30.939226519337, Blast_Score=162, Evalue=7e-40,
Organism=Homo sapiens, GI5454084, Length=292, Percent_Identity=29.1095890410959, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI1790046, Length=369, Percent_Identity=39.2953929539295, Blast_Score=248, Evalue=7e-67,
Organism=Escherichia coli, GI1786993, Length=358, Percent_Identity=41.340782122905, Blast_Score=233, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI71994529, Length=380, Percent_Identity=37.1052631578947, Blast_Score=253, Evalue=9e-68,
Organism=Caenorhabditis elegans, GI32566772, Length=362, Percent_Identity=28.1767955801105, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17560912, Length=367, Percent_Identity=27.7929155313352, Blast_Score=128, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17560914, Length=367, Percent_Identity=27.7929155313352, Blast_Score=127, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI71982617, Length=291, Percent_Identity=27.4914089347079, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI71982625, Length=291, Percent_Identity=27.4914089347079, Blast_Score=115, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6320438, Length=370, Percent_Identity=35.4054054054054, Blast_Score=201, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6320267, Length=361, Percent_Identity=31.3019390581717, Blast_Score=162, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6323954, Length=292, Percent_Identity=28.4246575342466, Blast_Score=114, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24662918, Length=382, Percent_Identity=36.9109947643979, Blast_Score=261, Evalue=4e-70,
Organism=Drosophila melanogaster, GI17137420, Length=351, Percent_Identity=36.4672364672365, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI17136286, Length=366, Percent_Identity=29.5081967213115, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24653280, Length=382, Percent_Identity=25.3926701570681, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24653276, Length=382, Percent_Identity=25.3926701570681, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24653278, Length=382, Percent_Identity=25.3926701570681, Blast_Score=126, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOF_ANAD2 (B8J637)

Other databases:

- EMBL:   CP001359
- RefSeq:   YP_002493998.1
- ProteinModelPortal:   B8J637
- GeneID:   7298724
- GenomeReviews:   CP001359_GR
- KEGG:   acp:A2cp1_3602
- HOGENOM:   HBG649839
- ProtClustDB:   CLSK946683
- HAMAP:   MF_01693
- InterPro:   IPR022834
- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- TIGRFAMs:   TIGR00858

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.3.1.47

Molecular weight: Translated: 41256; Mature: 41125

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: BINDING 23-23 BINDING 135-135 BINDING 181-181 BINDING 354-354

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARGALDWIGAELEALDAKGLRRSLEPIGPAQGPVVQVGGRALVNLCSNDYLGLAADPRV
CCCCCHHHHCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHCCCCCCCCCCCCH
RAAAADAAMRFGAGSGAARLVAGDLPPHGALEARLAAWKGREAALLFGSGYHANAGVPGA
HHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCE
LVGRDDAVFSDVLNHASIVDGCLLSRAELVRYRHCDVEELAGLLARTRARRKLVVTDAIF
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCEEEEEHHHH
SMDGDAAPLRELAELCDRHGAMLYVDEAHAAGVLGPNGAGLAEALGVQDRVDVHMGTLGK
HCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHH
ALGAFGAYVAGERRLIELLVSRARPFVFSTALPPPACAAALAALEVVATEPSRRTHLFAL
HHHHHHHHHHCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
CARMQAGLARLGFDVARVASPIFPVVLGTEARALAAAAALRERGWFVRAIRPPTVPHGTS
HHHHHHHHHHHCHHHHHHHCCHHHEEECCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCH
RLRVALSAAHDAAQVDGFLAALAAVLPDLPPAGPRRAG
HHEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
ARGALDWIGAELEALDAKGLRRSLEPIGPAQGPVVQVGGRALVNLCSNDYLGLAADPRV
CCCCHHHHCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHCCCCCCCCCCCCH
RAAAADAAMRFGAGSGAARLVAGDLPPHGALEARLAAWKGREAALLFGSGYHANAGVPGA
HHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCE
LVGRDDAVFSDVLNHASIVDGCLLSRAELVRYRHCDVEELAGLLARTRARRKLVVTDAIF
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCEEEEEHHHH
SMDGDAAPLRELAELCDRHGAMLYVDEAHAAGVLGPNGAGLAEALGVQDRVDVHMGTLGK
HCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHH
ALGAFGAYVAGERRLIELLVSRARPFVFSTALPPPACAAALAALEVVATEPSRRTHLFAL
HHHHHHHHHHCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
CARMQAGLARLGFDVARVASPIFPVVLGTEARALAAAAALRERGWFVRAIRPPTVPHGTS
HHHHHHHHHHHCHHHHHHHCCHHHEEECCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCH
RLRVALSAAHDAAQVDGFLAALAAVLPDLPPAGPRRAG
HHEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA