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The map label for this gene is dnaK [H]

Identifier: 220918328

GI number: 220918328

Start: 3591035

End: 3592918

Strand: Reverse

Name: dnaK [H]

Synonym: A2cp1_3232

Alternate gene names: 220918328

Gene position: 3592918-3591035 (Counterclockwise)

Preceding gene: 220918329

Following gene: 220918327

Centisome position: 71.44

GC content: 79.14

Gene sequence:

>1884_bases
ATGGCGGACGCCCCGCTGGCGGTCGGCATCGACCTCGGCACCACCAACTGCGCCATCGCCTGGCGCGCGCTGGAGGCCGG
CCCGGCGCCGGACGGCGCGCGCGCCGAGGCGCTCGCGATCCCGCAGCTCGTGCGCCCGGGCGAGGTGGAGCCGCGCCCGC
TGCTCCCGTCGTTCCTGTACCTGCCGCACCCGGCGGAGCTGCCGCCGGGGGCGACCGCCCTGCCGTGGGCGCCGGACGCC
GCGGGGGAGCTCGTGGGCGAGCTGGCGCGCGACCTGGGCGCGCAGACGCCCATCCGCCTCGTCTCCTCCGCCAAGAGCTG
GCTCTCGCACCCCACGCTCGACCGGCGCGCCGCGACGCTCCCGGCGGGCGCGCCGGCCGAGGTTCCTCGCCGCTCCCCGG
TGGAGGCCTCCGCCCGCTACCTCGCGCACCTGCGCGCGGCGTGGGACCGCGAGGTGGCCGCCGGGGATCCCGCCCGCGCG
CTGGCGGTCCAGCGGGTGACGCTGACCGTCCCCGCGTCCTTCGACGCCGTGGCGCGCGAGCTGACGGTGGAGGCGGCGCG
GCTCGCCGGGCTGCCGCAGGTGGTGCTGCTGGAGGAGCCGCAGGCCGCGCTCTACGCGTGGGTGGAGGCGATGGGCGAGG
GCTGGCGCAAGGCGGTCCGGCCCGGCGACCTGGTGCTGGTGGTGGACGTGGGCGGCGGCACCACCGACCTCTCGCTGGTC
GCGGTGGTGGATCGCGGCGGCGACCTCGGTCTCGAGCGCGTGGCGGTGGGCGACCACATCCTGCTCGGCGGCGACAACAT
GGACCTCGCGCTGGCGCACGCGCTCGCCGGCCGGCTGGAGCGCGACGGGCGCACGCTGGACCGCTGGCAGCTCCTCGCGC
TGGGGCACTCCGCCCGGCGCGCCAAGGAGGCGCTGTTCGCGGATCCGTCCCTGGACGAGCTGCCGGTCGCCATCCCCGGC
CGCGGCTCGTCGCTGGTCGGCGGCGCGCTGCGCACCGCGCTGGCCCGCGCCGAGCTGGAGGCGGTGCTCACCGACGGCTT
CTTCCCGCGCGTGGACGCGGACGCGAGGCCGCAGGCGCCGCGCCGTGTCGCGCTCACCACGCTCGGCCTGCCGTACGCCT
CGGACCCGGGCATCACCCGCCACCTCGCCGCGTTCCTCGCCCGCCACCGCGCCGCGCTCGGCTCGGGCGGGCACGCGGAG
GTCCCGCTCGCGGGCGGCGCGTTCCTCCACCCGACGGCCGTGCTGTTCAACGGCGGCGTCATGAAGGCCCCGCCGCTCGC
CGCGCGCGTCGTGGAGGTGCTGGCGGGCTGGCTCGCCGCCGAGGGCGCGCCGCCGCCCCGCGTGCTGCCGGCCGCCGAGC
TCGACCTGGCGGTGGCGCGCGGCGCGGCGGTGTACGGGCGCGTCCGCGCCGGCCACGGGATCCGGATCCGGGGCGGCACC
TCGCGTGCGTACTACGTCGGCGTGGAGGCGTCGATGCCGGCGGTGCCGGGCGTCGCGCCGCCGCTCCGGGCGGTGTGCCT
CGCGCCCATGGGCATGGAGGAGGGTACGCGCGCGGAGCTGCCGGGCGAGCCGCTCGGGTTGGTGGTGGGGGAGCCGGTGG
ACTTCCGGCTCTTCAGCTCCTCGGTCCGGCGCGACGACCGGCCCGGCGACGTGGTGGAGGACGCCGGCTCCCTCGAGGAG
GGGCCGCCGGTGACCGCCACCATCGCCGCCGGAACGCGCCCGGCCGGCGAGGTGGTGCCGGTGCGGCTGCGCGCGCACGT
CACCGAGATCGGGACGCTGGAGCTGGACTGCGTCGGTCCGGACGAGCGCTGGTCGCTGGAGTGGACCCTGCGCCAGCCGC
CTGGCGCCGGCGGCGAGCCGGAGCCGGCGGCCCCGCCCGCCTGA

Upstream 100 bases:

>100_bases
CACGGCTGGCGCGCCCGCGAGGTGAAGCTGCCGCCGCCGCCCGACGGCAAGGACCTGACCGTCGTGGCGCCGGCCGAGAT
CGAGCTGTGAGGGACGGCTG

Downstream 100 bases:

>100_bases
TCCGGAGCCACATGTCCGGCCCGCGCTACGTCGTCGGCATCGACCTCGGCACGGTCAACTCCGCGCTCGCCTCGGTGGAC
CTCGCGCGCGCGGGCGACCT

Product: heat-shock chaperone protein

Products: NA

Alternate protein names: HSP70; Heat shock 70 kDa protein; Heat shock protein 70 [H]

Number of amino acids: Translated: 627; Mature: 626

Protein sequence:

>627_residues
MADAPLAVGIDLGTTNCAIAWRALEAGPAPDGARAEALAIPQLVRPGEVEPRPLLPSFLYLPHPAELPPGATALPWAPDA
AGELVGELARDLGAQTPIRLVSSAKSWLSHPTLDRRAATLPAGAPAEVPRRSPVEASARYLAHLRAAWDREVAAGDPARA
LAVQRVTLTVPASFDAVARELTVEAARLAGLPQVVLLEEPQAALYAWVEAMGEGWRKAVRPGDLVLVVDVGGGTTDLSLV
AVVDRGGDLGLERVAVGDHILLGGDNMDLALAHALAGRLERDGRTLDRWQLLALGHSARRAKEALFADPSLDELPVAIPG
RGSSLVGGALRTALARAELEAVLTDGFFPRVDADARPQAPRRVALTTLGLPYASDPGITRHLAAFLARHRAALGSGGHAE
VPLAGGAFLHPTAVLFNGGVMKAPPLAARVVEVLAGWLAAEGAPPPRVLPAAELDLAVARGAAVYGRVRAGHGIRIRGGT
SRAYYVGVEASMPAVPGVAPPLRAVCLAPMGMEEGTRAELPGEPLGLVVGEPVDFRLFSSSVRRDDRPGDVVEDAGSLEE
GPPVTATIAAGTRPAGEVVPVRLRAHVTEIGTLELDCVGPDERWSLEWTLRQPPGAGGEPEPAAPPA

Sequences:

>Translated_627_residues
MADAPLAVGIDLGTTNCAIAWRALEAGPAPDGARAEALAIPQLVRPGEVEPRPLLPSFLYLPHPAELPPGATALPWAPDA
AGELVGELARDLGAQTPIRLVSSAKSWLSHPTLDRRAATLPAGAPAEVPRRSPVEASARYLAHLRAAWDREVAAGDPARA
LAVQRVTLTVPASFDAVARELTVEAARLAGLPQVVLLEEPQAALYAWVEAMGEGWRKAVRPGDLVLVVDVGGGTTDLSLV
AVVDRGGDLGLERVAVGDHILLGGDNMDLALAHALAGRLERDGRTLDRWQLLALGHSARRAKEALFADPSLDELPVAIPG
RGSSLVGGALRTALARAELEAVLTDGFFPRVDADARPQAPRRVALTTLGLPYASDPGITRHLAAFLARHRAALGSGGHAE
VPLAGGAFLHPTAVLFNGGVMKAPPLAARVVEVLAGWLAAEGAPPPRVLPAAELDLAVARGAAVYGRVRAGHGIRIRGGT
SRAYYVGVEASMPAVPGVAPPLRAVCLAPMGMEEGTRAELPGEPLGLVVGEPVDFRLFSSSVRRDDRPGDVVEDAGSLEE
GPPVTATIAAGTRPAGEVVPVRLRAHVTEIGTLELDCVGPDERWSLEWTLRQPPGAGGEPEPAAPPA
>Mature_626_residues
ADAPLAVGIDLGTTNCAIAWRALEAGPAPDGARAEALAIPQLVRPGEVEPRPLLPSFLYLPHPAELPPGATALPWAPDAA
GELVGELARDLGAQTPIRLVSSAKSWLSHPTLDRRAATLPAGAPAEVPRRSPVEASARYLAHLRAAWDREVAAGDPARAL
AVQRVTLTVPASFDAVARELTVEAARLAGLPQVVLLEEPQAALYAWVEAMGEGWRKAVRPGDLVLVVDVGGGTTDLSLVA
VVDRGGDLGLERVAVGDHILLGGDNMDLALAHALAGRLERDGRTLDRWQLLALGHSARRAKEALFADPSLDELPVAIPGR
GSSLVGGALRTALARAELEAVLTDGFFPRVDADARPQAPRRVALTTLGLPYASDPGITRHLAAFLARHRAALGSGGHAEV
PLAGGAFLHPTAVLFNGGVMKAPPLAARVVEVLAGWLAAEGAPPPRVLPAAELDLAVARGAAVYGRVRAGHGIRIRGGTS
RAYYVGVEASMPAVPGVAPPLRAVCLAPMGMEEGTRAELPGEPLGLVVGEPVDFRLFSSSVRRDDRPGDVVEDAGSLEEG
PPVTATIAAGTRPAGEVVPVRLRAHVTEIGTLELDCVGPDERWSLEWTLRQPPGAGGEPEPAAPPA

Specific function: Acts as a chaperone [H]

COG id: COG0443

COG function: function code O; Molecular chaperone

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the heat shock protein 70 family [H]

Homologues:

Organism=Escherichia coli, GI1788875, Length=273, Percent_Identity=32.967032967033, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1786196, Length=246, Percent_Identity=27.2357723577236, Blast_Score=69, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6320950, Length=361, Percent_Identity=26.3157894736842, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319396, Length=361, Percent_Identity=26.0387811634349, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012725
- InterPro:   IPR018181
- InterPro:   IPR001023
- InterPro:   IPR013126 [H]

Pfam domain/function: PF00012 HSP70 [H]

EC number: NA

Molecular weight: Translated: 65131; Mature: 65000

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00297 HSP70_1 ; PS00329 HSP70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADAPLAVGIDLGTTNCAIAWRALEAGPAPDGARAEALAIPQLVRPGEVEPRPLLPSFLY
CCCCCEEEEEECCCCCEEEEEEEHHCCCCCCCCCCHHHHCHHHCCCCCCCCCCCCCHHEE
LPHPAELPPGATALPWAPDAAGELVGELARDLGAQTPIRLVSSAKSWLSHPTLDRRAATL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHCCC
PAGAPAEVPRRSPVEASARYLAHLRAAWDREVAAGDPARALAVQRVTLTVPASFDAVARE
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHEEEEEEEEEECCCHHHHHHH
LTVEAARLAGLPQVVLLEEPQAALYAWVEAMGEGWRKAVRPGDLVLVVDVGGGTTDLSLV
HHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEE
AVVDRGGDLGLERVAVGDHILLGGDNMDLALAHALAGRLERDGRTLDRWQLLALGHSARR
EEEECCCCCCCEEEEECCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHEEHHHHCCHHHH
AKEALFADPSLDELPVAIPGRGSSLVGGALRTALARAELEAVLTDGFFPRVDADARPQAP
HHHHHCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
RRVALTTLGLPYASDPGITRHLAAFLARHRAALGSGGHAEVPLAGGAFLHPTAVLFNGGV
CEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEECCCCHHCCEEEEECCCC
MKAPPLAARVVEVLAGWLAAEGAPPPRVLPAAELDLAVARGAAVYGRVRAGHGIRIRGGT
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCEEEEECCC
SRAYYVGVEASMPAVPGVAPPLRAVCLAPMGMEEGTRAELPGEPLGLVVGEPVDFRLFSS
CEEEEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHH
SVRRDDRPGDVVEDAGSLEEGPPVTATIAAGTRPAGEVVPVRLRAHVTEIGTLELDCVGP
HHCCCCCCCCHHHCCCCCCCCCCEEEEEECCCCCCCCEEEEEEEEEEEECCCEEEEECCC
DERWSLEWTLRQPPGAGGEPEPAAPPA
CCCEEEEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ADAPLAVGIDLGTTNCAIAWRALEAGPAPDGARAEALAIPQLVRPGEVEPRPLLPSFLY
CCCCEEEEEECCCCCEEEEEEEHHCCCCCCCCCCHHHHCHHHCCCCCCCCCCCCCHHEE
LPHPAELPPGATALPWAPDAAGELVGELARDLGAQTPIRLVSSAKSWLSHPTLDRRAATL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHCCC
PAGAPAEVPRRSPVEASARYLAHLRAAWDREVAAGDPARALAVQRVTLTVPASFDAVARE
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHEEEEEEEEEECCCHHHHHHH
LTVEAARLAGLPQVVLLEEPQAALYAWVEAMGEGWRKAVRPGDLVLVVDVGGGTTDLSLV
HHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEE
AVVDRGGDLGLERVAVGDHILLGGDNMDLALAHALAGRLERDGRTLDRWQLLALGHSARR
EEEECCCCCCCEEEEECCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHEEHHHHCCHHHH
AKEALFADPSLDELPVAIPGRGSSLVGGALRTALARAELEAVLTDGFFPRVDADARPQAP
HHHHHCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
RRVALTTLGLPYASDPGITRHLAAFLARHRAALGSGGHAEVPLAGGAFLHPTAVLFNGGV
CEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEECCCCHHCCEEEEECCCC
MKAPPLAARVVEVLAGWLAAEGAPPPRVLPAAELDLAVARGAAVYGRVRAGHGIRIRGGT
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCEEEEECCC
SRAYYVGVEASMPAVPGVAPPLRAVCLAPMGMEEGTRAELPGEPLGLVVGEPVDFRLFSS
CEEEEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHH
SVRRDDRPGDVVEDAGSLEEGPPVTATIAAGTRPAGEVVPVRLRAHVTEIGTLELDCVGP
HHCCCCCCCCHHHCCCCCCCCCCEEEEEECCCCCCCCEEEEEEEEEEEECCCEEEEECCC
DERWSLEWTLRQPPGAGGEPEPAAPPA
CCCEEEEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12835416 [H]