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The map label for this gene is tolC [H]

Identifier: 220918262

GI number: 220918262

Start: 3504997

End: 3506334

Strand: Reverse

Name: tolC [H]

Synonym: A2cp1_3165

Alternate gene names: 220918262

Gene position: 3506334-3504997 (Counterclockwise)

Preceding gene: 220918263

Following gene: 220918257

Centisome position: 69.72

GC content: 79.67

Gene sequence:

>1338_bases
ATGACGAACGCCCCGCGGCTCGCTGCCCTCCTCCTGTTCGCCCCGGCGCTGGCGCTAGGGCAGGCCGCCCCGCCGCCGGC
CGCTGCGCCGCCTCCGGACGCGCCGGCCACCGACCGGCCCGGGGCCCGGCGCGTCCTCTCGCTCGAGGAGGCGGTGCGCA
CCGCCCGGGCACACCAGCCGCAGCTCCGCTCGGCGCGCGCCGCCACCGACGCGGCCCTCGCCCGCGCCGACCAGGCCCGG
GCGCCGCTCCTGCCCCAGCTCACCGGCAGCGCCGGGCTGGGCCGCACCACCGACAACTTCGCCGGGAAGCCGGGCGGCGC
GAGCTGGGGCACGAGCGGCACGTTCGACGCGCGCGCGGTGCTGAGCCAGACGCTCGTCGATCTCGGTCAGGTCGCCCGCT
GGCGCGCGGCCGGCGCGAGCGCCGACGCGCAGCGCGCCACCGAGCGCGCCACCGAGCTGGACGTGGTGGCCGGCGCGCAG
TCCGCGTTCTTCGTGGCGCGCGCCGCGCACGCGCTGGTGCGGGTGGCCCGCGAGACGCTCGACAACCAGGAGGCGCACCT
GCGCCAGGTGGAGGCGTTCGTGCAGGTCGGCACGCGCCCCGCCATCGACCTCGCCCAGGCGCGCGCCGATCGCGCGACCG
CGCAGGTCCAGCTGGTGCGCGCGGAGAACAGCTACGAGAACGCGCGGGCGCTCCTCGCCCAGGCCATCGGCCTGGACTGG
CCCGCCGACATCGAGCCGTCCGACGAGGCCATCGCGCCGGTGCCCGGCGAGGACGGGCCGCTCGCGCCGCTGGTCGCGGA
GGCGTCCTCGGCACGGCCGGAGCTGGCCTCGCTCGAGGCGCAGCGCCGCGCCGGGGCGCGCTCGCTCGACGCCGCGCGGG
CCGAGTGGCTGCCCACGCTCTCGGCGCAGACCGCGGTCACGGACGCCGGCACGCGCCCGGACGCGGACGGCCTCAACTGG
TCGGCCACGCTGAACCTGGGCTGGAACCTGCTCGAGGGCGGCGGCTCGCTGGCGCGGGCGCGGGAGGCGCGCGCCAACCT
GGCGGGCGTGGACGCGCAGGAGGCGGCGCTCCGCACGCAGATCACCGTCGACGTGGACGCCGCGCGCCAGGGCGTGCAGA
CCGCGCGCGCCGCCGCCGGGGCGGCCGACGAGGCGCTGGTCAACGCGCGCGAGCGCCTGCGGCTGGCCGAGGGCCGCTAC
CAGGCCGGCGCGGGCAGCATCATCGAGCTGGGCGACGCGCAGGTGGCGGCCACCTCCGCCGGGGCGCAGGTGGTGCAGGC
GGAGTACGACCTGGCCTCGGCGCGCGCGAGGCTGCTGCGCGCGCTGGGCCGCGGCTGA

Upstream 100 bases:

>100_bases
TGATCGCGGTCGGCTTCAGCGGGCTGGTGGGCGTCGCGTTCGGGCTCTACCCGGCCCGCCGCGCCTCCCGGCTCGATCCC
ATCCAGGCGCTGAGGTTCGA

Downstream 100 bases:

>100_bases
TCGCCGTGCCGCGCGACGCCGCGGTCAGCCCGAGTGCCGGGCGATCCCTCGCGCGACCGCGTCCACCAGGTACGGCCGCA
GGTCGGCGGGCGCGATGATC

Product: outer membrane efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MTNAPRLAALLLFAPALALGQAAPPPAAAPPPDAPATDRPGARRVLSLEEAVRTARAHQPQLRSARAATDAALARADQAR
APLLPQLTGSAGLGRTTDNFAGKPGGASWGTSGTFDARAVLSQTLVDLGQVARWRAAGASADAQRATERATELDVVAGAQ
SAFFVARAAHALVRVARETLDNQEAHLRQVEAFVQVGTRPAIDLAQARADRATAQVQLVRAENSYENARALLAQAIGLDW
PADIEPSDEAIAPVPGEDGPLAPLVAEASSARPELASLEAQRRAGARSLDAARAEWLPTLSAQTAVTDAGTRPDADGLNW
SATLNLGWNLLEGGGSLARAREARANLAGVDAQEAALRTQITVDVDAARQGVQTARAAAGAADEALVNARERLRLAEGRY
QAGAGSIIELGDAQVAATSAGAQVVQAEYDLASARARLLRALGRG

Sequences:

>Translated_445_residues
MTNAPRLAALLLFAPALALGQAAPPPAAAPPPDAPATDRPGARRVLSLEEAVRTARAHQPQLRSARAATDAALARADQAR
APLLPQLTGSAGLGRTTDNFAGKPGGASWGTSGTFDARAVLSQTLVDLGQVARWRAAGASADAQRATERATELDVVAGAQ
SAFFVARAAHALVRVARETLDNQEAHLRQVEAFVQVGTRPAIDLAQARADRATAQVQLVRAENSYENARALLAQAIGLDW
PADIEPSDEAIAPVPGEDGPLAPLVAEASSARPELASLEAQRRAGARSLDAARAEWLPTLSAQTAVTDAGTRPDADGLNW
SATLNLGWNLLEGGGSLARAREARANLAGVDAQEAALRTQITVDVDAARQGVQTARAAAGAADEALVNARERLRLAEGRY
QAGAGSIIELGDAQVAATSAGAQVVQAEYDLASARARLLRALGRG
>Mature_444_residues
TNAPRLAALLLFAPALALGQAAPPPAAAPPPDAPATDRPGARRVLSLEEAVRTARAHQPQLRSARAATDAALARADQARA
PLLPQLTGSAGLGRTTDNFAGKPGGASWGTSGTFDARAVLSQTLVDLGQVARWRAAGASADAQRATERATELDVVAGAQS
AFFVARAAHALVRVARETLDNQEAHLRQVEAFVQVGTRPAIDLAQARADRATAQVQLVRAENSYENARALLAQAIGLDWP
ADIEPSDEAIAPVPGEDGPLAPLVAEASSARPELASLEAQRRAGARSLDAARAEWLPTLSAQTAVTDAGTRPDADGLNWS
ATLNLGWNLLEGGGSLARAREARANLAGVDAQEAALRTQITVDVDAARQGVQTARAAAGAADEALVNARERLRLAEGRYQ
AGAGSIIELGDAQVAATSAGAQVVQAEYDLASARARLLRALGRG

Specific function: Required for proper expression of outer membrane protein genes such as ompF, nmpC, protein 2, hemolysin, colicin V, or colicin E1. May be specialized for signal sequence independent, extracellular secretion in Gram-negative bacteria [H]

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI87082199, Length=420, Percent_Identity=24.5238095238095, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010130 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 45943; Mature: 45811

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
0.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNAPRLAALLLFAPALALGQAAPPPAAAPPPDAPATDRPGARRVLSLEEAVRTARAHQP
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCH
QLRSARAATDAALARADQARAPLLPQLTGSAGLGRTTDNFAGKPGGASWGTSGTFDARAV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
LSQTLVDLGQVARWRAAGASADAQRATERATELDVVAGAQSAFFVARAAHALVRVARETL
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
DNQEAHLRQVEAFVQVGTRPAIDLAQARADRATAQVQLVRAENSYENARALLAQAIGLDW
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHCCCC
PADIEPSDEAIAPVPGEDGPLAPLVAEASSARPELASLEAQRRAGARSLDAARAEWLPTL
CCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCC
SAQTAVTDAGTRPDADGLNWSATLNLGWNLLEGGGSLARAREARANLAGVDAQEAALRTQ
CCHHHHCCCCCCCCCCCCCEEEEEECCCEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHE
ITVDVDAARQGVQTARAAAGAADEALVNARERLRLAEGRYQAGAGSIIELGDAQVAATSA
EEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEHHC
GAQVVQAEYDLASARARLLRALGRG
CHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TNAPRLAALLLFAPALALGQAAPPPAAAPPPDAPATDRPGARRVLSLEEAVRTARAHQP
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCH
QLRSARAATDAALARADQARAPLLPQLTGSAGLGRTTDNFAGKPGGASWGTSGTFDARAV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
LSQTLVDLGQVARWRAAGASADAQRATERATELDVVAGAQSAFFVARAAHALVRVARETL
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
DNQEAHLRQVEAFVQVGTRPAIDLAQARADRATAQVQLVRAENSYENARALLAQAIGLDW
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHCCCC
PADIEPSDEAIAPVPGEDGPLAPLVAEASSARPELASLEAQRRAGARSLDAARAEWLPTL
CCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCC
SAQTAVTDAGTRPDADGLNWSATLNLGWNLLEGGGSLARAREARANLAGVDAQEAALRTQ
CCHHHHCCCCCCCCCCCCCEEEEEECCCEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHE
ITVDVDAARQGVQTARAAAGAADEALVNARERLRLAEGRYQAGAGSIIELGDAQVAATSA
EEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEHHC
GAQVVQAEYDLASARARLLRALGRG
CHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 6312426; 2216730; 9278503; 6303857; 9298646; 9629924; 10879525 [H]