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The map label for this gene is slt [H]

Identifier: 220917448

GI number: 220917448

Start: 2601854

End: 2604145

Strand: Direct

Name: slt [H]

Synonym: A2cp1_2348

Alternate gene names: 220917448

Gene position: 2601854-2604145 (Clockwise)

Preceding gene: 220917446

Following gene: 220917451

Centisome position: 51.73

GC content: 82.11

Gene sequence:

>2292_bases
ATGCAGCCCGTCAAGGCCCCCACATCCCCCCTAGGGGTCCCCCTTCCCACCTGGGCGGAAAGCATCCGTAGGCCCTGGGC
TTACCTCGCTCGCGGGCGCGGTCGCCGCGCGCTCCTCGCCTGCACCCTGCTGCTGGCGGCCCGGCCCGCGTGCGCGGCCC
GGTCCGTCCCCGAGCCGCTCACCGACTGGCTCGCCGGCCTCGGTGACGCGGCCCAGGCCCTGGCCGGCGGGCAGGCGGAC
GCGTCCGCCGCGGCGGCGCGCCGCGCGTTCTCCGCGCGGCCGCGGGGGGCGGCAGGCGCGCGCGCGCAGGCGGCGCTGGG
CCTCGCGCTCGCGGAGGCCGGCCGGCCCTCGGAGGCGGCCGAGGCGCTGGAGGTGGCGCTCGCCCCGGCGATCGCGCCGG
CGCGGCCGCACCTCGCGTTCCTGCGCGGCCAGGCGCTGCTCGCGTCGGGCGAGCCGCACGCGGCGGCGCGGCTGCTGGAG
CAGGCGGGCGCCGACCTGCGCCTCGCGGTCGCGCGGCGCGCCCGCTTCCTCGCCGGCGAGGCGCTGGTCGAGGCGGGGCT
CCCCGCCGAGGCGGTGGCCACGCTCGACGCGGCGCTGCGCGCCTGGCCCACCGATCCCGCCGCGGCGGCGGCGCGGCTCG
CCCTCGGCCGCGCGCTGCGCGCCGCCGGCGACGAGGCGCGGGCGGCGGAGACCTGGCGCGCGCTCTGGCTCGAGGCGGAC
CTGCCGGAGGCGCGGGGCGCGGGCGAGCTGCTCGCGGCCTGGCGCGCCGCGGGCGGCCCGGTGCCGCTCGCCACCGCCGA
CGATCACCTGGCGCGGGCCGAGCGGCTGGTCGCGACCGCCCGGCCCGACGACGCGCTCCAGGAGCTCGCCGCGGCGCGCG
AGGCCGACCCCGAGGTGCCGGCGGGCCGCGTCGAGGTGCTGCGCGCCGCGGCGCTGGTCGGCCTCGGGCGGCACGCCGAG
GCGGCGAGGGTCGCGGCGCCGTTCGCGGACGATCCCGACCCGCGGGTGCAGCGCTCGGCGCGCCTCGCGCTGGCGCGGGC
CGCGGCGCGGGCCGGCCGCGTCGACGAGGCCAGCCGGCGCTACCGAGAGGTGGCCGCCTCCGACGCGCCCATCGCCGGGC
TGCCCGAGTGGCGCCAGCGCGACCTGGGCGACGAGGCGGCGTACCTCGCGGCCTGGCTCCACTACGACGCGGGCGACTAC
CGCCGCGCGGCGGCGGAGCTGGACGCGTTCGCGCGCGCCTACCGCCGCACCTCGCGGCGCGTGGACGAGGCGCTCTGGTT
CGCGGCCTGGTCGCGCTACCGGCTCGGCCGCACCGCCGACGCCGCGCGCGCCTTCGCCCGCCTGTCGCGCGGGCCGTTCG
CAGACGCCGCCGCCTACTGGCAGGGCCGCCTGGCGAAGGACGCGCGCCGCGGCGCCAGGCTCCTGCGCGCCGCCGCGGCC
GGCGCCGACCCCTGGTACGCGCTGCTCGCCCGCGCCCGGCTCGAGGCGGCCGGGATCGCCGCGGCGCCGCCTGCGGCGTC
GCCCGCGCGGCCGCTGCCCGACGCCGCGCCGGCCGCCGCCGCCGGGCAGCTCGCCGTCGCGGTCGAGCTGATGGGGCTCG
GGCTCGAGGACGAGGGGCTGGACGAGCTCCGGCAGCTCGCCCGCGGCGGCGCCGCCCGCGCGGCCGCGCCGCTCGTGGCG
CAGCTCGCGGCCCACGCCGGCGACGCGGAGCTGCCCTTCCGCATGGCGCGCGACTTCCTCGGGCAGAGCGCGCGCACGCT
GCGCTGGTCCCACCCCGAGCCGTACCCGGAGCTGCTCCCGGCCCGCGCGCGCGCGTTCGGCGTGGACCCGGCGCTGGTGC
TGGCGGTGATGCGGCGCGAGTCGAGCTTCCGCCGCGCGGTGCGGAGCGGCGCCGGGGCCGAGGGGCTGCTGCAGCTCCGG
CCGGTCACCGCCGAGCGGCTGGCGGCGCTGCTGGGCGTCCCCGGCGGCGTCGGCGACCGGCTCGACCGGCCCGAGGTGAA
CGTGGCGCTCGGGGCGCACTACCTCGCCCTGCTCGGCGCGCGCTTCGCGGACCCGGCGGTGGTGCTGGCCGCCTACAACG
CCGGCCCGGGGCCGGCCTCGGACTGGGCCCGGTCTCGGGCCGGCATGCCGCTCGACGCGTGGGTGGAGTGCATCCCGTAC
CGCGAGACGCGCCAGTACGTGAAGGTCGTGCTGTCGGACTGGGACGTCTACCGCCGGCTCTCCGGCGGCCCGGCGGCGCC
CATCGACCCCGCGCGGCCGGTGGCGGCGCCGGCCCCCGGCGTCGGGTTCTAG

Upstream 100 bases:

>100_bases
CTCCCCGAGGCATGGCGCGCCGCGTTCCGAAGTTGAAAGACTCGAAGCTCTCGCCCGGCCGACAGGCGGCCAGGCTGCGC
GCGCTATATCGCGCTCCCGC

Downstream 100 bases:

>100_bases
GCGACCGACCTACCCGGCCGCCTTCAGGGCGGCGGCGATCGTCTCCCCCATGCGCCCGAGCACCGACGGCGTGACGCCCG
GGATGGCGGCGAGCGCCTCG

Product: lytic transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 763; Mature: 763

Protein sequence:

>763_residues
MQPVKAPTSPLGVPLPTWAESIRRPWAYLARGRGRRALLACTLLLAARPACAARSVPEPLTDWLAGLGDAAQALAGGQAD
ASAAAARRAFSARPRGAAGARAQAALGLALAEAGRPSEAAEALEVALAPAIAPARPHLAFLRGQALLASGEPHAAARLLE
QAGADLRLAVARRARFLAGEALVEAGLPAEAVATLDAALRAWPTDPAAAAARLALGRALRAAGDEARAAETWRALWLEAD
LPEARGAGELLAAWRAAGGPVPLATADDHLARAERLVATARPDDALQELAAAREADPEVPAGRVEVLRAAALVGLGRHAE
AARVAAPFADDPDPRVQRSARLALARAAARAGRVDEASRRYREVAASDAPIAGLPEWRQRDLGDEAAYLAAWLHYDAGDY
RRAAAELDAFARAYRRTSRRVDEALWFAAWSRYRLGRTADAARAFARLSRGPFADAAAYWQGRLAKDARRGARLLRAAAA
GADPWYALLARARLEAAGIAAAPPAASPARPLPDAAPAAAAGQLAVAVELMGLGLEDEGLDELRQLARGGAARAAAPLVA
QLAAHAGDAELPFRMARDFLGQSARTLRWSHPEPYPELLPARARAFGVDPALVLAVMRRESSFRRAVRSGAGAEGLLQLR
PVTAERLAALLGVPGGVGDRLDRPEVNVALGAHYLALLGARFADPAVVLAAYNAGPGPASDWARSRAGMPLDAWVECIPY
RETRQYVKVVLSDWDVYRRLSGGPAAPIDPARPVAAPAPGVGF

Sequences:

>Translated_763_residues
MQPVKAPTSPLGVPLPTWAESIRRPWAYLARGRGRRALLACTLLLAARPACAARSVPEPLTDWLAGLGDAAQALAGGQAD
ASAAAARRAFSARPRGAAGARAQAALGLALAEAGRPSEAAEALEVALAPAIAPARPHLAFLRGQALLASGEPHAAARLLE
QAGADLRLAVARRARFLAGEALVEAGLPAEAVATLDAALRAWPTDPAAAAARLALGRALRAAGDEARAAETWRALWLEAD
LPEARGAGELLAAWRAAGGPVPLATADDHLARAERLVATARPDDALQELAAAREADPEVPAGRVEVLRAAALVGLGRHAE
AARVAAPFADDPDPRVQRSARLALARAAARAGRVDEASRRYREVAASDAPIAGLPEWRQRDLGDEAAYLAAWLHYDAGDY
RRAAAELDAFARAYRRTSRRVDEALWFAAWSRYRLGRTADAARAFARLSRGPFADAAAYWQGRLAKDARRGARLLRAAAA
GADPWYALLARARLEAAGIAAAPPAASPARPLPDAAPAAAAGQLAVAVELMGLGLEDEGLDELRQLARGGAARAAAPLVA
QLAAHAGDAELPFRMARDFLGQSARTLRWSHPEPYPELLPARARAFGVDPALVLAVMRRESSFRRAVRSGAGAEGLLQLR
PVTAERLAALLGVPGGVGDRLDRPEVNVALGAHYLALLGARFADPAVVLAAYNAGPGPASDWARSRAGMPLDAWVECIPY
RETRQYVKVVLSDWDVYRRLSGGPAAPIDPARPVAAPAPGVGF
>Mature_763_residues
MQPVKAPTSPLGVPLPTWAESIRRPWAYLARGRGRRALLACTLLLAARPACAARSVPEPLTDWLAGLGDAAQALAGGQAD
ASAAAARRAFSARPRGAAGARAQAALGLALAEAGRPSEAAEALEVALAPAIAPARPHLAFLRGQALLASGEPHAAARLLE
QAGADLRLAVARRARFLAGEALVEAGLPAEAVATLDAALRAWPTDPAAAAARLALGRALRAAGDEARAAETWRALWLEAD
LPEARGAGELLAAWRAAGGPVPLATADDHLARAERLVATARPDDALQELAAAREADPEVPAGRVEVLRAAALVGLGRHAE
AARVAAPFADDPDPRVQRSARLALARAAARAGRVDEASRRYREVAASDAPIAGLPEWRQRDLGDEAAYLAAWLHYDAGDY
RRAAAELDAFARAYRRTSRRVDEALWFAAWSRYRLGRTADAARAFARLSRGPFADAAAYWQGRLAKDARRGARLLRAAAA
GADPWYALLARARLEAAGIAAAPPAASPARPLPDAAPAAAAGQLAVAVELMGLGLEDEGLDELRQLARGGAARAAAPLVA
QLAAHAGDAELPFRMARDFLGQSARTLRWSHPEPYPELLPARARAFGVDPALVLAVMRRESSFRRAVRSGAGAEGLLQLR
PVTAERLAALLGVPGGVGDRLDRPEVNVALGAHYLALLGARFADPAVVLAAYNAGPGPASDWARSRAGMPLDAWVECIPY
RETRQYVKVVLSDWDVYRRLSGGPAAPIDPARPVAAPAPGVGF

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=164, Percent_Identity=35.9756097560976, Blast_Score=91, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 79881; Mature: 79881

Theoretical pI: Translated: 10.26; Mature: 10.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPVKAPTSPLGVPLPTWAESIRRPWAYLARGRGRRALLACTLLLAARPACAARSVPEPL
CCCCCCCCCCCCCCCHHHHHHHHCHHHHHHCCCCCHHHHHHHHHHHCCCHHHHCCCCHHH
TDWLAGLGDAAQALAGGQADASAAAARRAFSARPRGAAGARAQAALGLALAEAGRPSEAA
HHHHHCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCHHHH
EALEVALAPAIAPARPHLAFLRGQALLASGEPHAAARLLEQAGADLRLAVARRARFLAGE
HHHHHHHHHHCCCCCCHHHHHHCCHHEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
ALVEAGLPAEAVATLDAALRAWPTDPAAAAARLALGRALRAAGDEARAAETWRALWLEAD
HHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEECC
LPEARGAGELLAAWRAAGGPVPLATADDHLARAERLVATARPDDALQELAAAREADPEVP
CCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCC
AGRVEVLRAAALVGLGRHAEAARVAAPFADDPDPRVQRSARLALARAAARAGRVDEASRR
CHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHH
YREVAASDAPIAGLPEWRQRDLGDEAAYLAAWLHYDAGDYRRAAAELDAFARAYRRTSRR
HHHHHCCCCCCCCCCHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
VDEALWFAAWSRYRLGRTADAARAFARLSRGPFADAAAYWQGRLAKDARRGARLLRAAAA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
GADPWYALLARARLEAAGIAAAPPAASPARPLPDAAPAAAAGQLAVAVELMGLGLEDEGL
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCH
DELRQLARGGAARAAAPLVAQLAAHAGDAELPFRMARDFLGQSARTLRWSHPEPYPELLP
HHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEECCCCCCCHHHHH
ARARAFGVDPALVLAVMRRESSFRRAVRSGAGAEGLLQLRPVTAERLAALLGVPGGVGDR
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHCCCCCCCCC
LDRPEVNVALGAHYLALLGARFADPAVVLAAYNAGPGPASDWARSRAGMPLDAWVECIPY
CCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHCCCCHHHHHHHCCC
RETRQYVKVVLSDWDVYRRLSGGPAAPIDPARPVAAPAPGVGF
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQPVKAPTSPLGVPLPTWAESIRRPWAYLARGRGRRALLACTLLLAARPACAARSVPEPL
CCCCCCCCCCCCCCCHHHHHHHHCHHHHHHCCCCCHHHHHHHHHHHCCCHHHHCCCCHHH
TDWLAGLGDAAQALAGGQADASAAAARRAFSARPRGAAGARAQAALGLALAEAGRPSEAA
HHHHHCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCHHHH
EALEVALAPAIAPARPHLAFLRGQALLASGEPHAAARLLEQAGADLRLAVARRARFLAGE
HHHHHHHHHHCCCCCCHHHHHHCCHHEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
ALVEAGLPAEAVATLDAALRAWPTDPAAAAARLALGRALRAAGDEARAAETWRALWLEAD
HHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEECC
LPEARGAGELLAAWRAAGGPVPLATADDHLARAERLVATARPDDALQELAAAREADPEVP
CCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCC
AGRVEVLRAAALVGLGRHAEAARVAAPFADDPDPRVQRSARLALARAAARAGRVDEASRR
CHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHH
YREVAASDAPIAGLPEWRQRDLGDEAAYLAAWLHYDAGDYRRAAAELDAFARAYRRTSRR
HHHHHCCCCCCCCCCHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
VDEALWFAAWSRYRLGRTADAARAFARLSRGPFADAAAYWQGRLAKDARRGARLLRAAAA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
GADPWYALLARARLEAAGIAAAPPAASPARPLPDAAPAAAAGQLAVAVELMGLGLEDEGL
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCH
DELRQLARGGAARAAAPLVAQLAAHAGDAELPFRMARDFLGQSARTLRWSHPEPYPELLP
HHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEECCCCCCCHHHHH
ARARAFGVDPALVLAVMRRESSFRRAVRSGAGAEGLLQLRPVTAERLAALLGVPGGVGDR
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHCCCCCCCCC
LDRPEVNVALGAHYLALLGARFADPAVVLAAYNAGPGPASDWARSRAGMPLDAWVECIPY
CCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHCCCCHHHHHHHCCC
RETRQYVKVVLSDWDVYRRLSGGPAAPIDPARPVAAPAPGVGF
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]