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The map label for this gene is sigW [H]

Identifier: 220917367

GI number: 220917367

Start: 2518072

End: 2518662

Strand: Reverse

Name: sigW [H]

Synonym: A2cp1_2267

Alternate gene names: 220917367

Gene position: 2518662-2518072 (Counterclockwise)

Preceding gene: 220917368

Following gene: 220917366

Centisome position: 50.08

GC content: 75.13

Gene sequence:

>591_bases
TTGGGTCCCTCACTGTCCGGCGAGGCCGCGTGGACGCGGGCGGCCGCGCGAGGCGACCGGCAGGCCTTCTCACGGCTGGT
GGACCTGCACAAGCGCACGGTGTTCGCCCTCTGCGTCCGGCTGCTGCGGGACCAGGACGAGGCGCAGGACGCCGCCCAGG
AGGCGTTCGCGCGCGCCTACGCGAGCCTGGCCGCGTTCGATGCCTCGCAGCCGTTCGCGCCCTGGCTGCTGCGCATCGCC
CGCAACCACTGCCTCGACGTGCTCCGCCGGCGCCTGCCGCAGGCGCAGCGGGTCGAGCTGGACGCGGACCCGGAGGACGG
CGCGCCGCGCGACCTCGCCGACCCGGACGCGCCCCGCGGCGACGACGCGCTGGAGCGCCGCGAGCTGGCGCGCACGATCG
AGGCCGCGGTCGCGGCCCTGCCGGCGAACTACCGCGAGGTCGTGCACCTCTTTCACGTGGAGCACCTCTCCTACAAGGAG
ATCGCCGCCGCCATGGACGTCCCCATCGGCACCGTGATGACCTGGCTGCACCGCGCCCGCGCGCGGCTCAAGGCGACCCT
CGACGCGAGCGCCCCGGAGATCACGCCATGA

Upstream 100 bases:

>100_bases
GAAATAGGACCCGCCGTTCTCCGTAGGGTTCTGCATGGTTGCGGTGGCCGCACTCCCCGATCTAGCTTTCGCGCTCACCT
GTCCCGCAGAGGAGCGCTCG

Downstream 100 bases:

>100_bases
CGACCTTCACCGGACACCTCACCGACGCGCAGGCGCAGCGCCTGATGGACGGGATGCTGCTCGAGGCCGAGGCGGACGAG
GTCCTGGCGCACGCCGGCGG

Product: ECF subfamily RNA polymerase sigma-24 subunit

Products: NA

Alternate protein names: Sigma-W factor [H]

Number of amino acids: Translated: 196; Mature: 195

Protein sequence:

>196_residues
MGPSLSGEAAWTRAAARGDRQAFSRLVDLHKRTVFALCVRLLRDQDEAQDAAQEAFARAYASLAAFDASQPFAPWLLRIA
RNHCLDVLRRRLPQAQRVELDADPEDGAPRDLADPDAPRGDDALERRELARTIEAAVAALPANYREVVHLFHVEHLSYKE
IAAAMDVPIGTVMTWLHRARARLKATLDASAPEITP

Sequences:

>Translated_196_residues
MGPSLSGEAAWTRAAARGDRQAFSRLVDLHKRTVFALCVRLLRDQDEAQDAAQEAFARAYASLAAFDASQPFAPWLLRIA
RNHCLDVLRRRLPQAQRVELDADPEDGAPRDLADPDAPRGDDALERRELARTIEAAVAALPANYREVVHLFHVEHLSYKE
IAAAMDVPIGTVMTWLHRARARLKATLDASAPEITP
>Mature_195_residues
GPSLSGEAAWTRAAARGDRQAFSRLVDLHKRTVFALCVRLLRDQDEAQDAAQEAFARAYASLAAFDASQPFAPWLLRIAR
NHCLDVLRRRLPQAQRVELDADPEDGAPRDLADPDAPRGDDALERRELARTIEAAVAALPANYREVVHLFHVEHLSYKEI
AAAMDVPIGTVMTWLHRARARLKATLDASAPEITP

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Sigma-W controls genes involved in transport processes and detoxification [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=169, Percent_Identity=35.5029585798817, Blast_Score=84, Evalue=5e-18,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014294
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 21723; Mature: 21592

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGPSLSGEAAWTRAAARGDRQAFSRLVDLHKRTVFALCVRLLRDQDEAQDAAQEAFARAY
CCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
ASLAAFDASQPFAPWLLRIARNHCLDVLRRRLPQAQRVELDADPEDGAPRDLADPDAPRG
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHEECCCCCCCCCCCCCCCCCCCCCC
DDALERRELARTIEAAVAALPANYREVVHLFHVEHLSYKEIAAAMDVPIGTVMTWLHRAR
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ARLKATLDASAPEITP
HHHHHHHCCCCCCCCC
>Mature Secondary Structure 
GPSLSGEAAWTRAAARGDRQAFSRLVDLHKRTVFALCVRLLRDQDEAQDAAQEAFARAY
CCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
ASLAAFDASQPFAPWLLRIARNHCLDVLRRRLPQAQRVELDADPEDGAPRDLADPDAPRG
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHEECCCCCCCCCCCCCCCCCCCCCC
DDALERRELARTIEAAVAALPANYREVVHLFHVEHLSYKEIAAAMDVPIGTVMTWLHRAR
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ARLKATLDASAPEITP
HHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274029; 9384377; 12207695 [H]