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The map label for this gene is rluB [H]

Identifier: 220917363

GI number: 220917363

Start: 2512697

End: 2513671

Strand: Direct

Name: rluB [H]

Synonym: A2cp1_2263

Alternate gene names: 220917363

Gene position: 2512697-2513671 (Clockwise)

Preceding gene: 220917362

Following gene: 220917364

Centisome position: 49.96

GC content: 78.56

Gene sequence:

>975_bases
ATGGCCGAGGAGCGGCTGCAGAAGTTCCTGGCGGGGGCGGGCATCGCCTCGCGGCGCAAGGCGGAGGCGCTCATCAGCGC
CGGCCGCGTGCGCGTCAACCAGCGCACGGTGACCGAGCTGGGCACCAAGGTGGACCCGGGGCGCGACCTCGTCACGGTGG
ACGGCGCGCTCGTCTCGCAGCCGGAGCAGCGCAGCTACTACCTGCTCTACAAGCCGTCCGGCTGCGTCACCACCGCGTCG
GATCCGCAGGGTCGCCCCACCGCGATGGAGTACCTGCGCGGCGTGCCGGGGCGGCCGTTCCCGGTGGGCCGGCTCGACTA
CGACGCGGAGGGCGCGCTGCTGCTCACCGACGATGGCGAGCTGGCGAACCGCCTGGCCCACCCGCGCTACGGCCATCAGC
GCGTGTACCTGGTGAAGGTGAAGGGCGATCCGCCGGCGGAGGCGCTGGAGCGGATGAAGGCCGGGGTCCGGCTGGAGGAC
GGCCCGGCGCGCGCGCTGGAGGTGGCCGTCCACGAGCACGCCGACCGGAACGTGTGGATCCGCGTGGTGGTGGGCGAGGG
CCGCTTCCACCTGGTGAAGCGGCTCTGCGAGGCGGTGGGGCTGCAGGTCCAGCGCCTGTTCCGGCCGGAGTTCGGCGGCA
TCGGCGTCGGCGGGCTGCGGCCCGGCAGGTTCCGCCGGCTGGAGCCGGAGGAGGTGCGCGCGCTGCGGCAGCGGGTCGGG
CTCGAGTCCGGCACGCCGGCCCGGGGACCGTCGGTGCGCGAGCTCCCGAAGGCGGCCCGCCGCCACGGCCACGGGCCGCC
GGCGGCGCCCGGCGCGGCCCCGCCGCCGGCCGAGCCGGACGCGGACGAGGTCGGGCCGCGGGCTCGCGGCGCGAGCCGCG
CCGGTCCGAGGGCCGCCGCCCCCGGCCCCGGCGGCCGCGCGTCCCGGCCGTCGCGCCCGTCGCGCCCGGGCCGCGCCGGC
ACCCGCCGCCGCTGA

Upstream 100 bases:

>100_bases
TCGCCGACAAGAAGCGGGCCGACGAGGCCGAGGCGCAGGCCGACGCGGAGCCCGGCGCCGGCGCCGGGCCCGGTGGACCG
CCGGCGGCGGGGAGCTAGCG

Downstream 100 bases:

>100_bases
CCAAGGGCGCTGGCCGCCGCGGGCGCCCGCGCTTGCGCGCGGCGGGGTTCGCGGCGAGCATGGCGGCCCGTGTCCCGCGT
TCCCGCAGCCGCCACCTTCG

Product: pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MAEERLQKFLAGAGIASRRKAEALISAGRVRVNQRTVTELGTKVDPGRDLVTVDGALVSQPEQRSYYLLYKPSGCVTTAS
DPQGRPTAMEYLRGVPGRPFPVGRLDYDAEGALLLTDDGELANRLAHPRYGHQRVYLVKVKGDPPAEALERMKAGVRLED
GPARALEVAVHEHADRNVWIRVVVGEGRFHLVKRLCEAVGLQVQRLFRPEFGGIGVGGLRPGRFRRLEPEEVRALRQRVG
LESGTPARGPSVRELPKAARRHGHGPPAAPGAAPPPAEPDADEVGPRARGASRAGPRAAAPGPGGRASRPSRPSRPGRAG
TRRR

Sequences:

>Translated_324_residues
MAEERLQKFLAGAGIASRRKAEALISAGRVRVNQRTVTELGTKVDPGRDLVTVDGALVSQPEQRSYYLLYKPSGCVTTAS
DPQGRPTAMEYLRGVPGRPFPVGRLDYDAEGALLLTDDGELANRLAHPRYGHQRVYLVKVKGDPPAEALERMKAGVRLED
GPARALEVAVHEHADRNVWIRVVVGEGRFHLVKRLCEAVGLQVQRLFRPEFGGIGVGGLRPGRFRRLEPEEVRALRQRVG
LESGTPARGPSVRELPKAARRHGHGPPAAPGAAPPPAEPDADEVGPRARGASRAGPRAAAPGPGGRASRPSRPSRPGRAG
TRRR
>Mature_323_residues
AEERLQKFLAGAGIASRRKAEALISAGRVRVNQRTVTELGTKVDPGRDLVTVDGALVSQPEQRSYYLLYKPSGCVTTASD
PQGRPTAMEYLRGVPGRPFPVGRLDYDAEGALLLTDDGELANRLAHPRYGHQRVYLVKVKGDPPAEALERMKAGVRLEDG
PARALEVAVHEHADRNVWIRVVVGEGRFHLVKRLCEAVGLQVQRLFRPEFGGIGVGGLRPGRFRRLEPEEVRALRQRVGL
ESGTPARGPSVRELPKAARRHGHGPPAAPGAAPPPAEPDADEVGPRARGASRAGPRAAAPGPGGRASRPSRPSRPGRAGT
RRR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2633 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=246, Percent_Identity=42.6829268292683, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1788510, Length=238, Percent_Identity=34.453781512605, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1790453, Length=237, Percent_Identity=31.2236286919831, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI87081838, Length=177, Percent_Identity=33.8983050847458, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 34931; Mature: 34800

Theoretical pI: Translated: 11.37; Mature: 11.37

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEERLQKFLAGAGIASRRKAEALISAGRVRVNQRTVTELGTKVDPGRDLVTVDGALVSQ
CCHHHHHHHHHCCCHHHHHHHHHHHHHCCEEECHHHHHHHCCCCCCCCCEEEECCHHHCC
PEQRSYYLLYKPSGCVTTASDPQGRPTAMEYLRGVPGRPFPVGRLDYDAEGALLLTDDGE
CCCCCEEEEECCCCCEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCHH
LANRLAHPRYGHQRVYLVKVKGDPPAEALERMKAGVRLEDGPARALEVAVHEHADRNVWI
HHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCEECCCCCHHHHHHHHHCCCCCEEE
RVVVGEGRFHLVKRLCEAVGLQVQRLFRPEFGGIGVGGLRPGRFRRLEPEEVRALRQRVG
EEEECCCHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHC
LESGTPARGPSVRELPKAARRHGHGPPAAPGAAPPPAEPDADEVGPRARGASRAGPRAAA
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCC
PGPGGRASRPSRPSRPGRAGTRRR
CCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AEERLQKFLAGAGIASRRKAEALISAGRVRVNQRTVTELGTKVDPGRDLVTVDGALVSQ
CHHHHHHHHHCCCHHHHHHHHHHHHHCCEEECHHHHHHHCCCCCCCCCEEEECCHHHCC
PEQRSYYLLYKPSGCVTTASDPQGRPTAMEYLRGVPGRPFPVGRLDYDAEGALLLTDDGE
CCCCCEEEEECCCCCEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCHH
LANRLAHPRYGHQRVYLVKVKGDPPAEALERMKAGVRLEDGPARALEVAVHEHADRNVWI
HHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCEECCCCCHHHHHHHHHCCCCCEEE
RVVVGEGRFHLVKRLCEAVGLQVQRLFRPEFGGIGVGGLRPGRFRRLEPEEVRALRQRVG
EEEECCCHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHC
LESGTPARGPSVRELPKAARRHGHGPPAAPGAAPPPAEPDADEVGPRARGASRAGPRAAA
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCC
PGPGGRASRPSRPSRPGRAGTRRR
CCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 9888802 [H]