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The map label for this gene is lexA [H]

Identifier: 220917338

GI number: 220917338

Start: 2481960

End: 2482646

Strand: Reverse

Name: lexA [H]

Synonym: A2cp1_2238

Alternate gene names: 220917338

Gene position: 2482646-2481960 (Counterclockwise)

Preceding gene: 220917340

Following gene: 220917337

Centisome position: 49.36

GC content: 69.43

Gene sequence:

>687_bases
ATGGAAGGCCTCACCGACCGCCAGCTCGAGGTGCTCCGCTTCATCGCCTCGCAGATCGAGGACCACGGCTACCCGCCCAC
GATCCGCGAGATCGGCGAGGCGCTGGACATCCGCTCCACGAACGGCGTCAACGACCACCTCAAGGCGCTCGAGCGCAAGG
GCTACCTCTCGCGCGATCCGGTGAAGTCGCGCGCGCTCATCCCGACGTCGGCGGCGCGCGAGGCGCTCGGCGGCGGCGAG
ACCGGCTCCAACGTGGTCCCGCTGGTGCGCGGGCCGGCCCGGCCCGGCAGCCGGATGATCGAGATCCCCATCGTCGGCCG
CGTGGCCGCCGGCATGCCGATCCTCGCGCAGGAGCGCGTCGAGGACACGGTGCAGGTGGACGCGTTCCTGCTCGGCACGA
ACAAGAAGGTCTACGGGCTGCGCGTCCAGGGCGACTCGATGATCGGCGACGGGATCCTGCCCGGCGACTACGTGTTCGTG
AAGAAGCAGCTCAACGCGGACGACGGCGAGATCGTGGTCGCGATGATCGACGACGAGGCCACCGTGAAGCGCGTCTACTT
CGAGGGCGACCGCGTGCGCTTCCAGCCCTCGAACCCGCGCATGGCGCCCATCTACGTCCGCCACTCCGACTTCCGCAGCA
CCATGATCCTGGGCGTGGTGGTGGGCGTGTACCGCAAGCTCACCTGA

Upstream 100 bases:

>100_bases
CGTATCCCCGCCCCGCGCCCCGGGCAACCGGGCCGGCGGCCGGGCGTTTGCTTGTCTTCTGAACGCCGAGCCAGTATACC
GATGTTCAGGAGGTGCCCCC

Downstream 100 bases:

>100_bases
CCGCGCGGCGGCCGAGCCGCCGGGCGACGCTCGTACGTCCGCCACCTGCGACGGGTCCTGCCCACCAGGGCGAGCCCGCC
CTCGGCTTACCTTGCCCTCC

Product: SOS-response transcriptional repressor, LexA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MEGLTDRQLEVLRFIASQIEDHGYPPTIREIGEALDIRSTNGVNDHLKALERKGYLSRDPVKSRALIPTSAAREALGGGE
TGSNVVPLVRGPARPGSRMIEIPIVGRVAAGMPILAQERVEDTVQVDAFLLGTNKKVYGLRVQGDSMIGDGILPGDYVFV
KKQLNADDGEIVVAMIDDEATVKRVYFEGDRVRFQPSNPRMAPIYVRHSDFRSTMILGVVVGVYRKLT

Sequences:

>Translated_228_residues
MEGLTDRQLEVLRFIASQIEDHGYPPTIREIGEALDIRSTNGVNDHLKALERKGYLSRDPVKSRALIPTSAAREALGGGE
TGSNVVPLVRGPARPGSRMIEIPIVGRVAAGMPILAQERVEDTVQVDAFLLGTNKKVYGLRVQGDSMIGDGILPGDYVFV
KKQLNADDGEIVVAMIDDEATVKRVYFEGDRVRFQPSNPRMAPIYVRHSDFRSTMILGVVVGVYRKLT
>Mature_228_residues
MEGLTDRQLEVLRFIASQIEDHGYPPTIREIGEALDIRSTNGVNDHLKALERKGYLSRDPVKSRALIPTSAAREALGGGE
TGSNVVPLVRGPARPGSRMIEIPIVGRVAAGMPILAQERVEDTVQVDAFLLGTNKKVYGLRVQGDSMIGDGILPGDYVFV
KKQLNADDGEIVVAMIDDEATVKRVYFEGDRVRFQPSNPRMAPIYVRHSDFRSTMILGVVVGVYRKLT

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family [H]

Homologues:

Organism=Escherichia coli, GI1790476, Length=232, Percent_Identity=39.2241379310345, Blast_Score=127, Evalue=6e-31,
Organism=Escherichia coli, GI1787431, Length=120, Percent_Identity=31.6666666666667, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24 [H]

EC number: =3.4.21.88 [H]

Molecular weight: Translated: 25044; Mature: 25044

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGLTDRQLEVLRFIASQIEDHGYPPTIREIGEALDIRSTNGVNDHLKALERKGYLSRDP
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCC
VKSRALIPTSAAREALGGGETGSNVVPLVRGPARPGSRMIEIPIVGRVAAGMPILAQERV
CCCCCCCCCHHHHHHCCCCCCCCCEEEEEECCCCCCCEEEEEEEECHHHCCCCHHHHHHH
EDTVQVDAFLLGTNKKVYGLRVQGDSMIGDGILPGDYVFVKKQLNADDGEIVVAMIDDEA
HHHHEEEEEEEECCCEEEEEEEECCCEECCCCCCCCEEEEEEECCCCCCCEEEEEECCCC
TVKRVYFEGDRVRFQPSNPRMAPIYVRHSDFRSTMILGVVVGVYRKLT
EEEEEEEECCEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEGLTDRQLEVLRFIASQIEDHGYPPTIREIGEALDIRSTNGVNDHLKALERKGYLSRDP
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCC
VKSRALIPTSAAREALGGGETGSNVVPLVRGPARPGSRMIEIPIVGRVAAGMPILAQERV
CCCCCCCCCHHHHHHCCCCCCCCCEEEEEECCCCCCCEEEEEEEECHHHCCCCHHHHHHH
EDTVQVDAFLLGTNKKVYGLRVQGDSMIGDGILPGDYVFVKKQLNADDGEIVVAMIDDEA
HHHHEEEEEEEECCCEEEEEEEECCCEECCCCCCCCEEEEEEECCCCCCCEEEEEECCCC
TVKRVYFEGDRVRFQPSNPRMAPIYVRHSDFRSTMILGVVVGVYRKLT
EEEEEEEECCEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA