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The map label for this gene is pdhD [H]

Identifier: 220917211

GI number: 220917211

Start: 2350405

End: 2351859

Strand: Direct

Name: pdhD [H]

Synonym: A2cp1_2108

Alternate gene names: 220917211

Gene position: 2350405-2351859 (Clockwise)

Preceding gene: 220917210

Following gene: 220917212

Centisome position: 46.73

GC content: 71.75

Gene sequence:

>1455_bases
ATGCCCACCCAGACCTACGACGCCATCGTGATCGGCGCCGGCACCGGAGGCTACCCGGCCGCCATCCGCCTCGCCCAGCT
CGGCAAGAAGGTCGCGCTCATCGAGAAGGACGCCACCCTGGGCGGCGTCTGCCTGAACTGGGGGTGCATCCCGTCGAAGG
CGCTCATCGCGGCCGCCAACCTGGTGGACGAGATGCGCGGCGCCGCCGATCGCGGGATCATCGCCGAGCCGCCGCGCGTC
GACGTGGCGAAGCTGCGCGAGTTCAAGGACGGCGTGGTGAAGAAGCTCACCGGCGGCGTGGCGCTGCTCGAGAAGGGCAA
CGGCGTCGAGGTGGTGCGCGGGACCGCCACCGTGGTCGCGCCGAACGCGGTCGAGGTGGCCGGCAAGGACGGGCAGAAGA
CGCGGCTCGAGGCCGGCGCGATCCTGGTCGCCACCGGCGCGCGGCCCATCGAGATCCCGGGGTTCGCGTTCGACGGGAAG
GACGTCTGGAGCGCGCGGGAGGCGGTGGATCTCCCGGAGGTGCCGAAGCGCCTGGTCTGCATCGGCGGCGGCATCATCGG
CATGGAGCTCGGGACCGTCTACGCGAAGCTCGGCGCGCAGGTGACCTTCGTCGAGGCGCTGCCGCAGGTGCTCACCGGCG
TCGATCCCGACGCGGTGCGCCTGGTGCAGAAGGGGCTCCGCCAGCGCGGCGTGGCCGTGCACGTGAACGCGAAGGCGAAG
GGGTACGAGCGCCGCGGCAAGGAGCTGGTCGTCAAGATCGAGATCGAGGGCAAGGAGCAGGAGATCCCCTGCGACAAGAT
CCTGGTCGCGGTCGGCTTCAAGCCGAGCTCGGCCGGCTTCGGCCTCGAGCAGGTGGGCGTGAAGATCGGGCCGAAGGGCT
TCATCGAGGTCGATCAGCAGTACCGCACCAGCGTGCCGACCATCTTCGCCGCCGGCGACGTGACCGGGCCGCCGCTGCTC
GCGCACAAGGCGTCGAAGGAGGGCGAGATCGCCGCCGAGGTCATCGCGGGCCACAAGACCGTGCGCGACTGGGTGGGGAT
GCCCACCGCCATCTTCACCGACCCCGAGGTCGCCGCGGTGGGCCTCTCGGAGGAGGAGGCGCGCAAGCAGGGGTACGATC
CCATCGTCGGCAAGTTCGCGTTCGGGGCGCTGGGTCGCGCCATCGCCATCCACCACACCGAGGGCTTCGTGAAGGTGGTG
GGCGACCGCAAGACGAAGCTCCTGCTCGGCGCGAGCATCTGCGGGCCCGAGGCCGGCGACCTCATCGCCGAGGCCGCGCT
GGCGCTGGAGATGGGCGCGTACCTGGAGGACGTGGCGCTCACCATCCACGCCCACCCGACGCTCCCCGAGGCGCTCAACG
AGGCCTGCCGCGCCGCGCTCGGCGAGGCCATCCACATGCTGAACCGCCCGGAGCGCCCGAAGAAGGGCGAGGCCGCCGCG
GGCGCCCGGGCCTGA

Upstream 100 bases:

>100_bases
GTTTTGCGTTTACCCGGCCCCATCCCGCCGTTAGAGTCCGCGCGTGCGCGGGGACACCCGCGCTTCGCTCTTCCAGGTCC
TCCTTCATCGAGGGAACGCC

Downstream 100 bases:

>100_bases
CGCGCGCGCTCCGCCGGGCACCGGCGCCGCCACCCGGCGGTGCGCGGTCCCGCGGGGAGGGATCGATGCCGCGCGTCCTG
CGCACCTACCGCCTCGGCCG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex [H]

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MPTQTYDAIVIGAGTGGYPAAIRLAQLGKKVALIEKDATLGGVCLNWGCIPSKALIAAANLVDEMRGAADRGIIAEPPRV
DVAKLREFKDGVVKKLTGGVALLEKGNGVEVVRGTATVVAPNAVEVAGKDGQKTRLEAGAILVATGARPIEIPGFAFDGK
DVWSAREAVDLPEVPKRLVCIGGGIIGMELGTVYAKLGAQVTFVEALPQVLTGVDPDAVRLVQKGLRQRGVAVHVNAKAK
GYERRGKELVVKIEIEGKEQEIPCDKILVAVGFKPSSAGFGLEQVGVKIGPKGFIEVDQQYRTSVPTIFAAGDVTGPPLL
AHKASKEGEIAAEVIAGHKTVRDWVGMPTAIFTDPEVAAVGLSEEEARKQGYDPIVGKFAFGALGRAIAIHHTEGFVKVV
GDRKTKLLLGASICGPEAGDLIAEAALALEMGAYLEDVALTIHAHPTLPEALNEACRAALGEAIHMLNRPERPKKGEAAA
GARA

Sequences:

>Translated_484_residues
MPTQTYDAIVIGAGTGGYPAAIRLAQLGKKVALIEKDATLGGVCLNWGCIPSKALIAAANLVDEMRGAADRGIIAEPPRV
DVAKLREFKDGVVKKLTGGVALLEKGNGVEVVRGTATVVAPNAVEVAGKDGQKTRLEAGAILVATGARPIEIPGFAFDGK
DVWSAREAVDLPEVPKRLVCIGGGIIGMELGTVYAKLGAQVTFVEALPQVLTGVDPDAVRLVQKGLRQRGVAVHVNAKAK
GYERRGKELVVKIEIEGKEQEIPCDKILVAVGFKPSSAGFGLEQVGVKIGPKGFIEVDQQYRTSVPTIFAAGDVTGPPLL
AHKASKEGEIAAEVIAGHKTVRDWVGMPTAIFTDPEVAAVGLSEEEARKQGYDPIVGKFAFGALGRAIAIHHTEGFVKVV
GDRKTKLLLGASICGPEAGDLIAEAALALEMGAYLEDVALTIHAHPTLPEALNEACRAALGEAIHMLNRPERPKKGEAAA
GARA
>Mature_483_residues
PTQTYDAIVIGAGTGGYPAAIRLAQLGKKVALIEKDATLGGVCLNWGCIPSKALIAAANLVDEMRGAADRGIIAEPPRVD
VAKLREFKDGVVKKLTGGVALLEKGNGVEVVRGTATVVAPNAVEVAGKDGQKTRLEAGAILVATGARPIEIPGFAFDGKD
VWSAREAVDLPEVPKRLVCIGGGIIGMELGTVYAKLGAQVTFVEALPQVLTGVDPDAVRLVQKGLRQRGVAVHVNAKAKG
YERRGKELVVKIEIEGKEQEIPCDKILVAVGFKPSSAGFGLEQVGVKIGPKGFIEVDQQYRTSVPTIFAAGDVTGPPLLA
HKASKEGEIAAEVIAGHKTVRDWVGMPTAIFTDPEVAAVGLSEEEARKQGYDPIVGKFAFGALGRAIAIHHTEGFVKVVG
DRKTKLLLGASICGPEAGDLIAEAALALEMGAYLEDVALTIHAHPTLPEALNEACRAALGEAIHMLNRPERPKKGEAAAG
ARA

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=480, Percent_Identity=42.5, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI50301238, Length=469, Percent_Identity=29.2110874200426, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI22035672, Length=476, Percent_Identity=29.8319327731092, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI291045266, Length=457, Percent_Identity=27.3522975929978, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI148277065, Length=474, Percent_Identity=29.957805907173, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI148277071, Length=474, Percent_Identity=29.957805907173, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI33519430, Length=473, Percent_Identity=30.0211416490486, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI33519428, Length=473, Percent_Identity=30.0211416490486, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI33519426, Length=473, Percent_Identity=30.0211416490486, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI291045268, Length=449, Percent_Identity=24.9443207126949, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1786307, Length=470, Percent_Identity=41.2765957446808, Blast_Score=350, Evalue=2e-97,
Organism=Escherichia coli, GI87082354, Length=476, Percent_Identity=28.1512605042017, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI87081717, Length=454, Percent_Identity=27.7533039647577, Blast_Score=172, Evalue=6e-44,
Organism=Escherichia coli, GI1789915, Length=461, Percent_Identity=30.3687635574837, Blast_Score=157, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI32565766, Length=468, Percent_Identity=44.017094017094, Blast_Score=360, Evalue=1e-99,
Organism=Caenorhabditis elegans, GI17557007, Length=478, Percent_Identity=28.8702928870293, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI71983429, Length=437, Percent_Identity=28.3752860411899, Blast_Score=131, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI71983419, Length=454, Percent_Identity=29.0748898678414, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71982272, Length=482, Percent_Identity=27.1784232365145, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17559934, Length=258, Percent_Identity=26.7441860465116, Blast_Score=81, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321091, Length=476, Percent_Identity=42.0168067226891, Blast_Score=335, Evalue=1e-92,
Organism=Saccharomyces cerevisiae, GI6325240, Length=475, Percent_Identity=27.7894736842105, Blast_Score=179, Evalue=8e-46,
Organism=Saccharomyces cerevisiae, GI6325166, Length=465, Percent_Identity=30.1075268817204, Blast_Score=164, Evalue=2e-41,
Organism=Drosophila melanogaster, GI21358499, Length=471, Percent_Identity=43.9490445859873, Blast_Score=350, Evalue=1e-96,
Organism=Drosophila melanogaster, GI24640551, Length=484, Percent_Identity=30.3719008264463, Blast_Score=129, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24640549, Length=487, Percent_Identity=30.1848049281314, Blast_Score=129, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24640553, Length=487, Percent_Identity=30.1848049281314, Blast_Score=129, Evalue=6e-30,
Organism=Drosophila melanogaster, GI17737741, Length=484, Percent_Identity=27.6859504132231, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50533; Mature: 50402

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTQTYDAIVIGAGTGGYPAAIRLAQLGKKVALIEKDATLGGVCLNWGCIPSKALIAAAN
CCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCCCHHHHHHHHH
LVDEMRGAADRGIIAEPPRVDVAKLREFKDGVVKKLTGGVALLEKGNGVEVVRGTATVVA
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCEEEEC
PNAVEVAGKDGQKTRLEAGAILVATGARPIEIPGFAFDGKDVWSAREAVDLPEVPKRLVC
CCEEEECCCCCCCEEEECCEEEEEECCCEEECCCEEECCCHHHHHHHHCCCCCCCCEEEE
IGGGIIGMELGTVYAKLGAQVTFVEALPQVLTGVDPDAVRLVQKGLRQRGVAVHVNAKAK
ECCCEEEHHHHHHHHHHCCCEEHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEEEECCCC
GYERRGKELVVKIEIEGKEQEIPCDKILVAVGFKPSSAGFGLEQVGVKIGPKGFIEVDQQ
CHHCCCCEEEEEEEECCCCCCCCHHHEEEEECCCCCCCCCCHHHCCEEECCCCCEEECHH
YRTSVPTIFAAGDVTGPPLLAHKASKEGEIAAEVIAGHKTVRDWVGMPTAIFTDPEVAAV
HHCCCCEEEEECCCCCCCCEEECCCCCCCHHHHHHHCCHHHHHHCCCCEEEECCCCEEEE
GLSEEEARKQGYDPIVGKFAFGALGRAIAIHHTEGFVKVVGDRKTKLLLGASICGPEAGD
CCCHHHHHHCCCCCHHHHHHHHHHCCEEEEEECCCEEEEECCCCEEEEEEECCCCCCHHH
LIAEAALALEMGAYLEDVALTIHAHPTLPEALNEACRAALGEAIHMLNRPERPKKGEAAA
HHHHHHHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GARA
CCCC
>Mature Secondary Structure 
PTQTYDAIVIGAGTGGYPAAIRLAQLGKKVALIEKDATLGGVCLNWGCIPSKALIAAAN
CCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCCCHHHHHHHHH
LVDEMRGAADRGIIAEPPRVDVAKLREFKDGVVKKLTGGVALLEKGNGVEVVRGTATVVA
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEECCEEEEC
PNAVEVAGKDGQKTRLEAGAILVATGARPIEIPGFAFDGKDVWSAREAVDLPEVPKRLVC
CCEEEECCCCCCCEEEECCEEEEEECCCEEECCCEEECCCHHHHHHHHCCCCCCCCEEEE
IGGGIIGMELGTVYAKLGAQVTFVEALPQVLTGVDPDAVRLVQKGLRQRGVAVHVNAKAK
ECCCEEEHHHHHHHHHHCCCEEHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEEEECCCC
GYERRGKELVVKIEIEGKEQEIPCDKILVAVGFKPSSAGFGLEQVGVKIGPKGFIEVDQQ
CHHCCCCEEEEEEEECCCCCCCCHHHEEEEECCCCCCCCCCHHHCCEEECCCCCEEECHH
YRTSVPTIFAAGDVTGPPLLAHKASKEGEIAAEVIAGHKTVRDWVGMPTAIFTDPEVAAV
HHCCCCEEEEECCCCCCCCEEECCCCCCCHHHHHHHCCHHHHHHCCCCEEEECCCCEEEE
GLSEEEARKQGYDPIVGKFAFGALGRAIAIHHTEGFVKVVGDRKTKLLLGASICGPEAGD
CCCHHHHHHCCCCCHHHHHHHHHHCCEEEEEECCCEEEEECCCCEEEEEEECCCCCCHHH
LIAEAALALEMGAYLEDVALTIHAHPTLPEALNEACRAALGEAIHMLNRPERPKKGEAAA
HHHHHHHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GARA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2200674; 2253629; 6896188; 8805537 [H]