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The map label for this gene is lipA [H]

Identifier: 220917205

GI number: 220917205

Start: 2343986

End: 2344966

Strand: Direct

Name: lipA [H]

Synonym: A2cp1_2102

Alternate gene names: 220917205

Gene position: 2343986-2344966 (Clockwise)

Preceding gene: 220917202

Following gene: 220917206

Centisome position: 46.61

GC content: 73.19

Gene sequence:

>981_bases
ATGAGCGTACCCCGCAGCAGCCTGCCGGTCCTCGGCACCGACGCGCCCACCTCGCGGCGCGACATCGCGAGCGCGATGCG
CGAGCGCGGCGTCCCCATGGACGCGAAGAAGCCGAGCTGGCTCCGGGTGAACGTGCCGGGGGGCGAGCGGTACCAGAAGG
TCCGGGAGACGCTGAAGGGCCTCCAGCTCCACACCGTCTGCGCCGAGGCGCACTGCCCGAACGTGGCGGAGTGCTGGGGC
GGCGGCACCGCGACCGTCATGCTGATGGGCGACGTGTGCACCCGCGGCTGCCGCTTCTGCAACGTGAAGACGGCCGCCCA
CCCGCCCGCGCTCGATCCGGACGAGCCGCGCCACCTGGCCGAGGCCATCGCCGAGCTGGCGCTCGACTACATCGTCGTGA
CCAGCGTGGACCGCGACGACCTGCCCGACGGCGGCGCCGCGCACTTCGCCGACGCGATCCGGCGGCTGAAGGAGATCCCG
GGTCTGCTCGTCGAGGTGCTCACGCCGGACTTCCGCGGCGACCCGGAGGCCGTGCGCACCGTCGGCCGCGCCGCGCCCGA
CGTCTTCGCGAACAACCTCGAGACCGTGCGTCGGCTCACGCCCGCGGTCCGCGACGCGAAGGCCACCTACGACCAGACGC
TCGGCGTCCTCGCGCAGCTGAAGCGCGAGTTCCCGCAGGTGGTCACCAAGTCGTCGATCATGGTCGGCCTGGGCGAGCAG
GAGGCCGAGGTGGTCGAGGCGATGCGGGACCTCCGCGCGCACGGCGTCGAGATCCTCACGCTCGGCCAGTACCTGCGGCC
GAGCGCCTGGCACCTGCCGGTGGTGGAGTACGTGTCGCCCGAGCGCTTCGCGGCGTACCGGGACCAGGGGCTCGCCCTCG
GGTTCCGGTACGTGGCGAGCGGCCCGCTGGTCCGGTCGTCGTACCGCGCGGCGGAGCTGTTCCTGCGCGGCGAGATCGAG
TCGAGGACGAAGCCCCGCTGA

Upstream 100 bases:

>100_bases
TGCAGGTACGCTCCCCAATGTGTAGGGTAGCAGAGGGCGGGCGGGCAAAGGAGCGGCGCGTCTTGACCCGGCTCGAACCC
ATCGCTACATCGGCGGCGCA

Downstream 100 bases:

>100_bases
CGACAGGAGCGAGCATGCGCCGAGAGCACGTGGTGGGTCCGCCGACGAGGCATCACCCGCGCGAGGCACGGGACTACGAG
CCCGGCCGGTTCCTGAAGGA

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MSVPRSSLPVLGTDAPTSRRDIASAMRERGVPMDAKKPSWLRVNVPGGERYQKVRETLKGLQLHTVCAEAHCPNVAECWG
GGTATVMLMGDVCTRGCRFCNVKTAAHPPALDPDEPRHLAEAIAELALDYIVVTSVDRDDLPDGGAAHFADAIRRLKEIP
GLLVEVLTPDFRGDPEAVRTVGRAAPDVFANNLETVRRLTPAVRDAKATYDQTLGVLAQLKREFPQVVTKSSIMVGLGEQ
EAEVVEAMRDLRAHGVEILTLGQYLRPSAWHLPVVEYVSPERFAAYRDQGLALGFRYVASGPLVRSSYRAAELFLRGEIE
SRTKPR

Sequences:

>Translated_326_residues
MSVPRSSLPVLGTDAPTSRRDIASAMRERGVPMDAKKPSWLRVNVPGGERYQKVRETLKGLQLHTVCAEAHCPNVAECWG
GGTATVMLMGDVCTRGCRFCNVKTAAHPPALDPDEPRHLAEAIAELALDYIVVTSVDRDDLPDGGAAHFADAIRRLKEIP
GLLVEVLTPDFRGDPEAVRTVGRAAPDVFANNLETVRRLTPAVRDAKATYDQTLGVLAQLKREFPQVVTKSSIMVGLGEQ
EAEVVEAMRDLRAHGVEILTLGQYLRPSAWHLPVVEYVSPERFAAYRDQGLALGFRYVASGPLVRSSYRAAELFLRGEIE
SRTKPR
>Mature_325_residues
SVPRSSLPVLGTDAPTSRRDIASAMRERGVPMDAKKPSWLRVNVPGGERYQKVRETLKGLQLHTVCAEAHCPNVAECWGG
GTATVMLMGDVCTRGCRFCNVKTAAHPPALDPDEPRHLAEAIAELALDYIVVTSVDRDDLPDGGAAHFADAIRRLKEIPG
LLVEVLTPDFRGDPEAVRTVGRAAPDVFANNLETVRRLTPAVRDAKATYDQTLGVLAQLKREFPQVVTKSSIMVGLGEQE
AEVVEAMRDLRAHGVEILTLGQYLRPSAWHLPVVEYVSPERFAAYRDQGLALGFRYVASGPLVRSSYRAAELFLRGEIES
RTKPR

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=291, Percent_Identity=54.9828178694158, Blast_Score=338, Evalue=6e-93,
Organism=Homo sapiens, GI37577164, Length=243, Percent_Identity=55.5555555555556, Blast_Score=283, Evalue=2e-76,
Organism=Escherichia coli, GI1786846, Length=278, Percent_Identity=49.6402877697842, Blast_Score=272, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI32564533, Length=264, Percent_Identity=52.6515151515151, Blast_Score=292, Evalue=1e-79,
Organism=Saccharomyces cerevisiae, GI6324770, Length=297, Percent_Identity=50.8417508417508, Blast_Score=319, Evalue=3e-88,
Organism=Drosophila melanogaster, GI221513272, Length=304, Percent_Identity=53.6184210526316, Blast_Score=348, Evalue=2e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 35745; Mature: 35614

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVPRSSLPVLGTDAPTSRRDIASAMRERGVPMDAKKPSWLRVNVPGGERYQKVRETLKG
CCCCCCCCCEECCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHC
LQLHTVCAEAHCPNVAECWGGGTATVMLMGDVCTRGCRFCNVKTAAHPPALDPDEPRHLA
CHHHHHHHHHCCCCHHHHHCCCCEEEEEECHHHHCCCCEECCCCCCCCCCCCCCCHHHHH
EAIAELALDYIVVTSVDRDDLPDGGAAHFADAIRRLKEIPGLLVEVLTPDFRGDPEAVRT
HHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
VGRAAPDVFANNLETVRRLTPAVRDAKATYDQTLGVLAQLKREFPQVVTKSSIMVGLGEQ
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEECCCH
EAEVVEAMRDLRAHGVEILTLGQYLRPSAWHLPVVEYVSPERFAAYRDQGLALGFRYVAS
HHHHHHHHHHHHHCCEEEEEHHHHHCCCCCCCCHHHHCCHHHHHHHHCCCHHHHHHHHHC
GPLVRSSYRAAELFLRGEIESRTKPR
CCHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SVPRSSLPVLGTDAPTSRRDIASAMRERGVPMDAKKPSWLRVNVPGGERYQKVRETLKG
CCCCCCCCEECCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHC
LQLHTVCAEAHCPNVAECWGGGTATVMLMGDVCTRGCRFCNVKTAAHPPALDPDEPRHLA
CHHHHHHHHHCCCCHHHHHCCCCEEEEEECHHHHCCCCEECCCCCCCCCCCCCCCHHHHH
EAIAELALDYIVVTSVDRDDLPDGGAAHFADAIRRLKEIPGLLVEVLTPDFRGDPEAVRT
HHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
VGRAAPDVFANNLETVRRLTPAVRDAKATYDQTLGVLAQLKREFPQVVTKSSIMVGLGEQ
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEECCCH
EAEVVEAMRDLRAHGVEILTLGQYLRPSAWHLPVVEYVSPERFAAYRDQGLALGFRYVAS
HHHHHHHHHHHHHCCEEEEEHHHHHCCCCCCCCHHHHCCHHHHHHHHCCCHHHHHHHHHC
GPLVRSSYRAAELFLRGEIESRTKPR
CCHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA