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The map label for this gene is paaF [C]

Identifier: 220917197

GI number: 220917197

Start: 2334292

End: 2335068

Strand: Reverse

Name: paaF [C]

Synonym: A2cp1_2094

Alternate gene names: 220917197

Gene position: 2335068-2334292 (Counterclockwise)

Preceding gene: 220917198

Following gene: 220917193

Centisome position: 46.43

GC content: 72.07

Gene sequence:

>777_bases
ATGAACGACGCGCTCGTCAGGACCAGGAAGGAAGGCCGGATCGGCTGGATCACGCTCCACCGGCCGGAGGCGATGAACAC
CTTCATCGTCGCGTTCGCCGACCAGCTCGACCAGGCGCTGCGGCGGATGGAGGACGATCCGGAGGTGACGGTCGTCGTCG
TGGACGCGGCGGGGAAGCACTACTGCACCGGGATCGCGCTCGAGCAGTTCACGCCGCGGACGCAACGCGAGTACCGCGAG
CTCCTCCACCGGATCGACGCGTTCTACCGGACCCTCGCGCGGATGCGGACGGTGACGATCGCGGCGGTGCAGGGCTACGC
GGTGGCGAACGGCGCCGGGCTCGCGTTCGCCTGCGACCTCACCGTCGCGGCGGACACGGCGCGGTTCGGCACCACCGCCA
TCAACGTCGGCCTCATCTGCCTCGGCCCCGCGGCGGCGATGGCGCGGCTCGTCGGGCGGAAGAAGGCGGCGGAGCTGCTG
CTGACGGGCGAGCTGGTGAGCGCGGCGGACGCGCTGGCGCTCGGCCTCGTGAACCGGGTCGTGCCGGAGGCGTCGCTCGC
GGACGAGGCGCTGAAGCTCGCGCAGAAGCTCGCCGCGAAGAGCCCGCTCGCGCTGCGCATCGGCAAGGAGGGCCTGAACC
GGCTGCCGGACCTGCCCGCGCTCGAGCGCATCGACCTCGCCGACGATCTGTTCGCCACGCTCGCCGCCACCCAGGACGCG
GTGGAGGGCGTGAGCGCGTTCCTGGAGAAGCGTGCGCCCACCTGGAAGGAGCGCTAG

Upstream 100 bases:

>100_bases
ACGGCGCGGCCCAACCGGTGGCGCGCGCCCGGTGCGCCGGTCGTGAAAAGCTCGGGGGCGAGTACGCCGCCTCCACCCGC
TCCACCGCCCAGGGAATCCC

Downstream 100 bases:

>100_bases
GCCGGCGCCGGCGCCCGGGATCGGCCCGCCGCGAGCGCGGGACGCCGCCGCGCGTCACCGCGGGTAGTACGACACGGTGA
ACGCCGCGCCGTTGCGGGTC

Product: Enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MNDALVRTRKEGRIGWITLHRPEAMNTFIVAFADQLDQALRRMEDDPEVTVVVVDAAGKHYCTGIALEQFTPRTQREYRE
LLHRIDAFYRTLARMRTVTIAAVQGYAVANGAGLAFACDLTVAADTARFGTTAINVGLICLGPAAAMARLVGRKKAAELL
LTGELVSAADALALGLVNRVVPEASLADEALKLAQKLAAKSPLALRIGKEGLNRLPDLPALERIDLADDLFATLAATQDA
VEGVSAFLEKRAPTWKER

Sequences:

>Translated_258_residues
MNDALVRTRKEGRIGWITLHRPEAMNTFIVAFADQLDQALRRMEDDPEVTVVVVDAAGKHYCTGIALEQFTPRTQREYRE
LLHRIDAFYRTLARMRTVTIAAVQGYAVANGAGLAFACDLTVAADTARFGTTAINVGLICLGPAAAMARLVGRKKAAELL
LTGELVSAADALALGLVNRVVPEASLADEALKLAQKLAAKSPLALRIGKEGLNRLPDLPALERIDLADDLFATLAATQDA
VEGVSAFLEKRAPTWKER
>Mature_258_residues
MNDALVRTRKEGRIGWITLHRPEAMNTFIVAFADQLDQALRRMEDDPEVTVVVVDAAGKHYCTGIALEQFTPRTQREYRE
LLHRIDAFYRTLARMRTVTIAAVQGYAVANGAGLAFACDLTVAADTARFGTTAINVGLICLGPAAAMARLVGRKKAAELL
LTGELVSAADALALGLVNRVVPEASLADEALKLAQKLAAKSPLALRIGKEGLNRLPDLPALERIDLADDLFATLAATQDA
VEGVSAFLEKRAPTWKER

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=253, Percent_Identity=32.4110671936759, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI31542718, Length=248, Percent_Identity=34.2741935483871, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI70995211, Length=263, Percent_Identity=26.615969581749, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI4502327, Length=246, Percent_Identity=26.4227642276423, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI150378541, Length=247, Percent_Identity=26.3157894736842, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1787659, Length=260, Percent_Identity=35, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1788597, Length=245, Percent_Identity=33.469387755102, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI221142681, Length=274, Percent_Identity=31.3868613138686, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787660, Length=245, Percent_Identity=32.6530612244898, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI87082183, Length=251, Percent_Identity=26.2948207171315, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788682, Length=213, Percent_Identity=30.0469483568075, Blast_Score=74, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17554946, Length=259, Percent_Identity=30.8880308880309, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI25145438, Length=254, Percent_Identity=33.0708661417323, Blast_Score=128, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17540714, Length=208, Percent_Identity=31.25, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17560910, Length=251, Percent_Identity=26.6932270916335, Blast_Score=101, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17540306, Length=234, Percent_Identity=27.3504273504274, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17536985, Length=258, Percent_Identity=24.031007751938, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17534483, Length=264, Percent_Identity=25.3787878787879, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17549921, Length=218, Percent_Identity=28.8990825688073, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17535521, Length=258, Percent_Identity=26.7441860465116, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI20129971, Length=243, Percent_Identity=33.3333333333333, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24653477, Length=243, Percent_Identity=33.3333333333333, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI19922422, Length=256, Percent_Identity=28.125, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24653139, Length=249, Percent_Identity=27.710843373494, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21357171, Length=198, Percent_Identity=31.3131313131313, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24650670, Length=201, Percent_Identity=31.8407960199005, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI19920382, Length=249, Percent_Identity=25.7028112449799, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24654903, Length=190, Percent_Identity=25.7894736842105, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27892; Mature: 27892

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDALVRTRKEGRIGWITLHRPEAMNTFIVAFADQLDQALRRMEDDPEVTVVVVDAAGKH
CCCCHHEECCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
YCTGIALEQFTPRTQREYRELLHRIDAFYRTLARMRTVTIAAVQGYAVANGAGLAFACDL
HHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCEEEECCCCEEEEEEE
TVAADTARFGTTAINVGLICLGPAAAMARLVGRKKAAELLLTGELVSAADALALGLVNRV
EEECCCHHHCCHHHEECEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPEASLADEALKLAQKLAAKSPLALRIGKEGLNRLPDLPALERIDLADDLFATLAATQDA
CCCHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHH
VEGVSAFLEKRAPTWKER
HHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNDALVRTRKEGRIGWITLHRPEAMNTFIVAFADQLDQALRRMEDDPEVTVVVVDAAGKH
CCCCHHEECCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
YCTGIALEQFTPRTQREYRELLHRIDAFYRTLARMRTVTIAAVQGYAVANGAGLAFACDL
HHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCEEEECCCCEEEEEEE
TVAADTARFGTTAINVGLICLGPAAAMARLVGRKKAAELLLTGELVSAADALALGLVNRV
EEECCCHHHCCHHHEECEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPEASLADEALKLAQKLAAKSPLALRIGKEGLNRLPDLPALERIDLADDLFATLAATQDA
CCCHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHH
VEGVSAFLEKRAPTWKER
HHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA