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The map label for this gene is ilvB [H]

Identifier: 220917088

GI number: 220917088

Start: 2241896

End: 2243602

Strand: Direct

Name: ilvB [H]

Synonym: A2cp1_1985

Alternate gene names: 220917088

Gene position: 2241896-2243602 (Clockwise)

Preceding gene: 220917087

Following gene: 220917089

Centisome position: 44.58

GC content: 70.59

Gene sequence:

>1707_bases
ATGGCCAAGGAGAAGATGACGGGCGCGAAGGCGGTCCTGCGCGCCCTGGACGCGGAGGGGGTCGAGGTCCTCTTCGGCAT
CCCGGGCGGCGCGATCCTCCCGCTCTACGACGCGCTGTACGGGGTCGAGCGGCCCAAGCACGTGCTGGTGCGCCACGAGC
AGGTGGGCGGGCACGCCGCGGAGGGCTACGCCCACGCCACCGGCAAGGTGGGCGTGTGCATGGGCACCTCCGGCCCCGGC
GCGACCAACCTCGTGACCGCGATCGCCGACGCGTACATGGACTCGGTGCCCATGGTCGCGATCACCGCGAACGTGTCCTC
GAGCCTCATCGGCACCGACGCGTTCCAGGAGGCGGACATCACCGGCATCACCATGCCGATCACCAAGCACAACTGGCTGG
TGACCGACGTGCGCGAGCTGCCCCGCATCCTCAAGGAGGCGTTCTACGTGGCCCGCACCGGCCGGCCCGGCCCGGTGCTG
GTGGACATCCCCAAGGACGTCCTCAACGCCGAGCTCGAGTTCGAGTGGCCGGAGACGGTGGAGATCCCCGGCTACCACCC
GCCGGTCCGCGAGGAGCCGCGGCTCAAGGACGCCGCGCAGCTCATGCGCGCGTCCAGCCGGCCGGTGGTGTACCTGGGCG
GCGGCGTCCGTACCGCCGACGCGCACAAGGAGGTCTTCGCCTTCGCCGAGTTCCTCGGCGCGCCGGTGGTGACGACGCTC
CACGGCAAGGGCGCGTTCCCGGAGACCAGCCCGCTGTGCCTGGGCATGTTCGGCATGCACGGCTCGCGGTTCGCGAACTA
CACGGTGCAGGCGTCCGACCTGATCATCGCGCTCGGCGCCCGCTTCGACGACCGCGTCACCGGCAAGCTCTCCACGTTCG
CCCCCGAGGCGAAGGTGATCCACCTCGACGTGGACCCGGCGGAGATCTCGAAGCTGGTGACCGCGACGGTGCCGCTGGTG
GGCGACCTGAAGCGCCTGCTGCCGCAGCTCCACGAGGAGGCGCGGCGCGCGTTCGCCGACCACGGGCGCCCGGACCTCGC
GCCCTGGCGGAAGCGGGTGAACGACTGGCGCGAGAAGCACCCGCTCCGCTACCGCCAGGATCCCAAGCAGCCGCTGCTCC
CGCAGTACGTCATCGACACGCTCTGGAAGAAGACCGGCGGCAAGGCCATCGTCACCACCGGCGTGGGCGAGCACCAGATG
TTCGCGGCGCAGTGGTGGAAGACCGCCGAGCCCCGGACGTTCGTCACCTCCGGCGGCCTCGGGACCATGGGCTTCTGCCT
CCCGTCCGCGATCGGCATCCAGCTCGGCCGCCCGGACGCGCTGGTGATCGGCATCGACGGCGACGGCTCGTTCCAGATGA
CGCTGCAGGATCTCGCCACCGCGTCGGAGCTCCGGCTGCCCATCAAGATCTTCGTCCTGAACAACCTGTTCCTGGGGATG
GTCCGCCAGTGGCAGGAGCTGTTCTACGAGGAGAAGTACGCGGAGACGCCGCTCGCCGACCTCCCGGACCTGGTGAAGCT
CGCCGACGCCTACGGGTGCCTCGGCCTGCGCGCCCGGACCCCGGAGGAGCTGGACCAGGTCATCGACCGCGCGCTCGCGA
ACCAGGACGGCCCCACCATCGTGGACGTCCGGATCCGGCGCGAGGAGAAGGTGTACCCGATGGTGCCGGCCGGTGCCCCC
CTCAACGACATGATCGACGGTGAGTGA

Upstream 100 bases:

>100_bases
CGTCGCCGCCCCCCCGGGCGGCGACGCCGGGTCGTGCGTCGGCGTTCCGCTTCGACGACAGCGCAGCTCCCTCGAACACT
CGTGAATCGAAGGTGACGGC

Downstream 100 bases:

>100_bases
CATGGAACGGACCGAGACCCCGCAGCCGAACGGCTCCACCCACACCATCAGCCTCCTCGTCGAGAACAAGCCGGGCGTGC
TGCACCGGATCGCCGGGCTG

Product: acetolactate synthase, large subunit, biosynthetic type

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 568; Mature: 567

Protein sequence:

>568_residues
MAKEKMTGAKAVLRALDAEGVEVLFGIPGGAILPLYDALYGVERPKHVLVRHEQVGGHAAEGYAHATGKVGVCMGTSGPG
ATNLVTAIADAYMDSVPMVAITANVSSSLIGTDAFQEADITGITMPITKHNWLVTDVRELPRILKEAFYVARTGRPGPVL
VDIPKDVLNAELEFEWPETVEIPGYHPPVREEPRLKDAAQLMRASSRPVVYLGGGVRTADAHKEVFAFAEFLGAPVVTTL
HGKGAFPETSPLCLGMFGMHGSRFANYTVQASDLIIALGARFDDRVTGKLSTFAPEAKVIHLDVDPAEISKLVTATVPLV
GDLKRLLPQLHEEARRAFADHGRPDLAPWRKRVNDWREKHPLRYRQDPKQPLLPQYVIDTLWKKTGGKAIVTTGVGEHQM
FAAQWWKTAEPRTFVTSGGLGTMGFCLPSAIGIQLGRPDALVIGIDGDGSFQMTLQDLATASELRLPIKIFVLNNLFLGM
VRQWQELFYEEKYAETPLADLPDLVKLADAYGCLGLRARTPEELDQVIDRALANQDGPTIVDVRIRREEKVYPMVPAGAP
LNDMIDGE

Sequences:

>Translated_568_residues
MAKEKMTGAKAVLRALDAEGVEVLFGIPGGAILPLYDALYGVERPKHVLVRHEQVGGHAAEGYAHATGKVGVCMGTSGPG
ATNLVTAIADAYMDSVPMVAITANVSSSLIGTDAFQEADITGITMPITKHNWLVTDVRELPRILKEAFYVARTGRPGPVL
VDIPKDVLNAELEFEWPETVEIPGYHPPVREEPRLKDAAQLMRASSRPVVYLGGGVRTADAHKEVFAFAEFLGAPVVTTL
HGKGAFPETSPLCLGMFGMHGSRFANYTVQASDLIIALGARFDDRVTGKLSTFAPEAKVIHLDVDPAEISKLVTATVPLV
GDLKRLLPQLHEEARRAFADHGRPDLAPWRKRVNDWREKHPLRYRQDPKQPLLPQYVIDTLWKKTGGKAIVTTGVGEHQM
FAAQWWKTAEPRTFVTSGGLGTMGFCLPSAIGIQLGRPDALVIGIDGDGSFQMTLQDLATASELRLPIKIFVLNNLFLGM
VRQWQELFYEEKYAETPLADLPDLVKLADAYGCLGLRARTPEELDQVIDRALANQDGPTIVDVRIRREEKVYPMVPAGAP
LNDMIDGE
>Mature_567_residues
AKEKMTGAKAVLRALDAEGVEVLFGIPGGAILPLYDALYGVERPKHVLVRHEQVGGHAAEGYAHATGKVGVCMGTSGPGA
TNLVTAIADAYMDSVPMVAITANVSSSLIGTDAFQEADITGITMPITKHNWLVTDVRELPRILKEAFYVARTGRPGPVLV
DIPKDVLNAELEFEWPETVEIPGYHPPVREEPRLKDAAQLMRASSRPVVYLGGGVRTADAHKEVFAFAEFLGAPVVTTLH
GKGAFPETSPLCLGMFGMHGSRFANYTVQASDLIIALGARFDDRVTGKLSTFAPEAKVIHLDVDPAEISKLVTATVPLVG
DLKRLLPQLHEEARRAFADHGRPDLAPWRKRVNDWREKHPLRYRQDPKQPLLPQYVIDTLWKKTGGKAIVTTGVGEHQMF
AAQWWKTAEPRTFVTSGGLGTMGFCLPSAIGIQLGRPDALVIGIDGDGSFQMTLQDLATASELRLPIKIFVLNNLFLGMV
RQWQELFYEEKYAETPLADLPDLVKLADAYGCLGLRARTPEELDQVIDRALANQDGPTIVDVRIRREEKVYPMVPAGAPL
NDMIDGE

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=563, Percent_Identity=25.044404973357, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI21361361, Length=608, Percent_Identity=24.5065789473684, Blast_Score=115, Evalue=1e-25,
Organism=Escherichia coli, GI87081685, Length=566, Percent_Identity=45.583038869258, Blast_Score=530, Evalue=1e-151,
Organism=Escherichia coli, GI1790104, Length=573, Percent_Identity=44.5026178010471, Blast_Score=458, Evalue=1e-130,
Organism=Escherichia coli, GI1786717, Length=581, Percent_Identity=32.8743545611015, Blast_Score=322, Evalue=4e-89,
Organism=Escherichia coli, GI1787096, Length=562, Percent_Identity=31.1387900355872, Blast_Score=234, Evalue=2e-62,
Organism=Escherichia coli, GI1788716, Length=556, Percent_Identity=24.6402877697842, Blast_Score=132, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17531299, Length=571, Percent_Identity=24.1681260945709, Blast_Score=146, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17531301, Length=571, Percent_Identity=24.1681260945709, Blast_Score=145, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI17542570, Length=545, Percent_Identity=25.8715596330275, Blast_Score=113, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6323755, Length=583, Percent_Identity=44.4253859348199, Blast_Score=462, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6320816, Length=480, Percent_Identity=23.3333333333333, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321524, Length=491, Percent_Identity=23.8289205702648, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6323163, Length=489, Percent_Identity=23.3128834355828, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323073, Length=491, Percent_Identity=22.6069246435845, Blast_Score=84, Evalue=6e-17,
Organism=Drosophila melanogaster, GI19922626, Length=570, Percent_Identity=26.140350877193, Blast_Score=159, Evalue=4e-39,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62043; Mature: 61912

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKMTGAKAVLRALDAEGVEVLFGIPGGAILPLYDALYGVERPKHVLVRHEQVGGHAA
CCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCHHHEEEEHHCCCCCC
EGYAHATGKVGVCMGTSGPGATNLVTAIADAYMDSVPMVAITANVSSSLIGTDAFQEADI
CCCEECCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHCCCCCCCCCC
TGITMPITKHNWLVTDVRELPRILKEAFYVARTGRPGPVLVDIPKDVLNAELEFEWPETV
CEEEEEEECCCEEHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHCCCEEEECCCCEE
EIPGYHPPVREEPRLKDAAQLMRASSRPVVYLGGGVRTADAHKEVFAFAEFLGAPVVTTL
ECCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCEECCHHHHHHHHHHHHHCCCEEEEE
HGKGAFPETSPLCLGMFGMHGSRFANYTVQASDLIIALGARFDDRVTGKLSTFAPEAKVI
CCCCCCCCCCCHHEEECCCCCCCCCCEEEEECCEEEEECCCCCCCCCCCHHHCCCCCEEE
HLDVDPAEISKLVTATVPLVGDLKRLLPQLHEEARRAFADHGRPDLAPWRKRVNDWREKH
EEECCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHC
PLRYRQDPKQPLLPQYVIDTLWKKTGGKAIVTTGVGEHQMFAAQWWKTAEPRTFVTSGGL
CCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCEEEECCCC
GTMGFCLPSAIGIQLGRPDALVIGIDGDGSFQMTLQDLATASELRLPIKIFVLNNLFLGM
CHHHHHHHHHHCEEECCCCEEEEEECCCCCEEEEHHHHHHHHHCCCCEEEEEHHHHHHHH
VRQWQELFYEEKYAETPLADLPDLVKLADAYGCLGLRARTPEELDQVIDRALANQDGPTI
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCEE
VDVRIRREEKVYPMVPAGAPLNDMIDGE
EEEEEECCCCCCEECCCCCCHHHCCCCC
>Mature Secondary Structure 
AKEKMTGAKAVLRALDAEGVEVLFGIPGGAILPLYDALYGVERPKHVLVRHEQVGGHAA
CCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCHHHEEEEHHCCCCCC
EGYAHATGKVGVCMGTSGPGATNLVTAIADAYMDSVPMVAITANVSSSLIGTDAFQEADI
CCCEECCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHCCCCCCCCCC
TGITMPITKHNWLVTDVRELPRILKEAFYVARTGRPGPVLVDIPKDVLNAELEFEWPETV
CEEEEEEECCCEEHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHCCCEEEECCCCEE
EIPGYHPPVREEPRLKDAAQLMRASSRPVVYLGGGVRTADAHKEVFAFAEFLGAPVVTTL
ECCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCEECCHHHHHHHHHHHHHCCCEEEEE
HGKGAFPETSPLCLGMFGMHGSRFANYTVQASDLIIALGARFDDRVTGKLSTFAPEAKVI
CCCCCCCCCCCHHEEECCCCCCCCCCEEEEECCEEEEECCCCCCCCCCCHHHCCCCCEEE
HLDVDPAEISKLVTATVPLVGDLKRLLPQLHEEARRAFADHGRPDLAPWRKRVNDWREKH
EEECCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHC
PLRYRQDPKQPLLPQYVIDTLWKKTGGKAIVTTGVGEHQMFAAQWWKTAEPRTFVTSGGL
CCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCEEEECCCC
GTMGFCLPSAIGIQLGRPDALVIGIDGDGSFQMTLQDLATASELRLPIKIFVLNNLFLGM
CHHHHHHHHHHCEEECCCCEEEEEECCCCCEEEEHHHHHHHHHCCCCEEEEEHHHHHHHH
VRQWQELFYEEKYAETPLADLPDLVKLADAYGCLGLRARTPEELDQVIDRALANQDGPTI
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCEE
VDVRIRREEKVYPMVPAGAPLNDMIDGE
EEEEEECCCCCCEECCCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8366043; 12948626 [H]