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The map label for this gene is sigB [H]

Identifier: 220917075

GI number: 220917075

Start: 2227079

End: 2228002

Strand: Reverse

Name: sigB [H]

Synonym: A2cp1_1972

Alternate gene names: 220917075

Gene position: 2228002-2227079 (Counterclockwise)

Preceding gene: 220917079

Following gene: 220917072

Centisome position: 44.3

GC content: 68.18

Gene sequence:

>924_bases
ATGAAGCGCCAGAGCGTCCCCGGTACCGCGAGCTCCCTCGAGGTCTACCTCTCGGAGATCAATCGGTTTCCGCTGCTGAC
GGTCGAGGACGAGCAGCGGCTGGCCCGGCAGTACTGCGCAGATCGCGACACCCGCGCCGCCCACCGGCTGGTCACCGCGA
ACCTCCGCTTCGTGGTCAAGGTCGCCTACGAATACCGCTCCTACGGGTTCCGCATGGCCGACCTCATCCAGGAGGGCAAC
ATCGGCCTGATGAAGGCGGTCCAGAAGTTCGATCCGGACAAGGGCATCCGCCTCATCTCGTACGCGGTCTGGTGGATCCG
CGCGTACATCCAGAACTACATCCTCAAGTCCTGGTCGCTGGTGAAGCTCGGGACGACGCAGGCGCAGCGCAAGCTGTTCT
TCTCGCTGGCCCGGACGAAGCGCGAGCTCGACAAGCGCTCCCAGGAGCACGGCGCCGACTCCGACGGGCACGACGCGAAC
AAGGTGGCGAAGAAGCTCCGCGTGAAGCCCGGCGAGGTCCGCGAGATGGAGCAGCGGATGGAGGGGCGCGACCTGTCGCT
CGACGCGCCCATGGGCGACGACGGTGGCTACTCCCACGTGGACTTCCTCCCCTCCGGCACCGCCTCGCAGGACGACGAGC
TGTCCGGCGTGCAGGAGCAGAAGGTCGTCTCCGGCCGGGTCGTCGAGGCGCTGGGCCGCCTCGACCAGCGCGAGCGCTAC
ATCATCGAGATGCGCGTGATGAGCGACCGCCCGCTCACGCTCAAGGAGCTGGGCGAGCACTTCGGCTTCTCGCGCGAGCG
CGCCCGGCAGCTCGAGATCCGCGCCAAGGAGAAGCTGAAGGAGGAGCTGCGCGCGCTCGCGACCGAGATCGACTGGCCCA
CCGACGGCGCGCCGGTGGACATCGACGACCGCGCGCTGGCCTGA

Upstream 100 bases:

>100_bases
CAACCACTCAGGATCACGGCCGAAAGGCGCTTGACCCCAGACGGCACATGGGTTGCTTAGGGGCCTACTTGCTGCCACCG
CCACGCACCATGGAGGTCCG

Downstream 100 bases:

>100_bases
CCGCGGGCTCGCCCGGCCCTGGGCCGCCCAGGGCCGGCGCTACGCCATGCCGTGCTTCTTGAGGCGGTAGCGGAGCGTGT
CGCGCGACACGCCCAGCAGG

Product: RNA polymerase sigma-32 subunit RpoH

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MKRQSVPGTASSLEVYLSEINRFPLLTVEDEQRLARQYCADRDTRAAHRLVTANLRFVVKVAYEYRSYGFRMADLIQEGN
IGLMKAVQKFDPDKGIRLISYAVWWIRAYIQNYILKSWSLVKLGTTQAQRKLFFSLARTKRELDKRSQEHGADSDGHDAN
KVAKKLRVKPGEVREMEQRMEGRDLSLDAPMGDDGGYSHVDFLPSGTASQDDELSGVQEQKVVSGRVVEALGRLDQRERY
IIEMRVMSDRPLTLKELGEHFGFSRERARQLEIRAKEKLKEELRALATEIDWPTDGAPVDIDDRALA

Sequences:

>Translated_307_residues
MKRQSVPGTASSLEVYLSEINRFPLLTVEDEQRLARQYCADRDTRAAHRLVTANLRFVVKVAYEYRSYGFRMADLIQEGN
IGLMKAVQKFDPDKGIRLISYAVWWIRAYIQNYILKSWSLVKLGTTQAQRKLFFSLARTKRELDKRSQEHGADSDGHDAN
KVAKKLRVKPGEVREMEQRMEGRDLSLDAPMGDDGGYSHVDFLPSGTASQDDELSGVQEQKVVSGRVVEALGRLDQRERY
IIEMRVMSDRPLTLKELGEHFGFSRERARQLEIRAKEKLKEELRALATEIDWPTDGAPVDIDDRALA
>Mature_307_residues
MKRQSVPGTASSLEVYLSEINRFPLLTVEDEQRLARQYCADRDTRAAHRLVTANLRFVVKVAYEYRSYGFRMADLIQEGN
IGLMKAVQKFDPDKGIRLISYAVWWIRAYIQNYILKSWSLVKLGTTQAQRKLFFSLARTKRELDKRSQEHGADSDGHDAN
KVAKKLRVKPGEVREMEQRMEGRDLSLDAPMGDDGGYSHVDFLPSGTASQDDELSGVQEQKVVSGRVVEALGRLDQRERY
IIEMRVMSDRPLTLKELGEHFGFSRERARQLEIRAKEKLKEELRALATEIDWPTDGAPVDIDDRALA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=295, Percent_Identity=38.3050847457627, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1789098, Length=287, Percent_Identity=32.404181184669, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1789448, Length=243, Percent_Identity=30.8641975308642, Blast_Score=106, Evalue=2e-24,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 35164; Mature: 35164

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRQSVPGTASSLEVYLSEINRFPLLTVEDEQRLARQYCADRDTRAAHRLVTANLRFVVK
CCCCCCCCCHHHHHHHHHHHCCCCEEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
VAYEYRSYGFRMADLIQEGNIGLMKAVQKFDPDKGIRLISYAVWWIRAYIQNYILKSWSL
HHHHHHHHCHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCE
VKLGTTQAQRKLFFSLARTKRELDKRSQEHGADSDGHDANKVAKKLRVKPGEVREMEQRM
EEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHH
EGRDLSLDAPMGDDGGYSHVDFLPSGTASQDDELSGVQEQKVVSGRVVEALGRLDQRERY
CCCCCEECCCCCCCCCCCCEEECCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHE
IIEMRVMSDRPLTLKELGEHFGFSRERARQLEIRAKEKLKEELRALATEIDWPTDGAPVD
EEEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
IDDRALA
CCCCCCC
>Mature Secondary Structure
MKRQSVPGTASSLEVYLSEINRFPLLTVEDEQRLARQYCADRDTRAAHRLVTANLRFVVK
CCCCCCCCCHHHHHHHHHHHCCCCEEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
VAYEYRSYGFRMADLIQEGNIGLMKAVQKFDPDKGIRLISYAVWWIRAYIQNYILKSWSL
HHHHHHHHCHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCE
VKLGTTQAQRKLFFSLARTKRELDKRSQEHGADSDGHDANKVAKKLRVKPGEVREMEQRM
EEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHH
EGRDLSLDAPMGDDGGYSHVDFLPSGTASQDDELSGVQEQKVVSGRVVEALGRLDQRERY
CCCCCEECCCCCCCCCCCCEEECCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHE
IIEMRVMSDRPLTLKELGEHFGFSRERARQLEIRAKEKLKEELRALATEIDWPTDGAPVD
EEEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
IDDRALA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1475207 [H]