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The map label for this gene is amtB [C]

Identifier: 220916833

GI number: 220916833

Start: 1929024

End: 1930547

Strand: Reverse

Name: amtB [C]

Synonym: A2cp1_1729

Alternate gene names: 220916833

Gene position: 1930547-1929024 (Counterclockwise)

Preceding gene: 220916835

Following gene: 220916830

Centisome position: 38.39

GC content: 70.73

Gene sequence:

>1524_bases
ATGAAGAAGCTCCGAGCCTTGCTCCTCCTCGCAGCGCTGGCCGCCCCGGCCGCGCTGCTCCTCCACGCCCCGGCCGCGCG
CGCCGACGACGCCGCCGCGGCGGCGCCGGTCGCGTTCACGCAGGGCATGGCCGACGCCATCGCCGCCCAGAAGGTCGGGC
TCGACACGATCTGGGTGATGATCGCGGCGTTCCTGGTCTTCTTCATGAACCTCGGCTTCGCGCTGGTCGAGTCCGGCTTC
TGCCGCGCGAAGAACACCGTGAACATCCTGTTCAAGAACTTCGTGGTGTTCGCGATCTCGTCGCTCGCGTTCCTGGTGAT
CGGGTACGGCCTGATGTTCGGGGACGGCAACCCGTTCTTCGGCACGAGCGGGCTCCTGTTCGCGTCCGGCGCGGACAACA
GCCCCGCGCTCGGCGACGCGTACCAGGGCGTCTACCACGCGCTCAACTGGACCGGCGTGCCGCTGTGGGCGAAGTTCTTC
TTCCAGCTCGTGTTCGCCGGCACCGCCGCCACCATCGTGTCCGGCGCGGTCGCCGAGCGGATCAAGTTCAAGGCCTTCAT
CGTCTTCACGCTGTTCCTGGTGGGCGTGGTCTACCCGGTCGGCGGCCACATGATCTGGGGCGGCGGCTGGCTCGCCAGCA
AGGGCTTCCTCGACTTCGCCGGCTCGACGGTGGTGCACTCGATCGGCGGCTGGGCGGCGCTCGCCGGCATCCTCGTGCTC
GGGCCGCGCCTCGGGAAGTACGGCCCCGGCAAGCAGATCAACGTCATCCCCGGCCACAACATGACGAGCGCCGCCATCGG
CGTGTTCGTGCTGTGGTTCGGCTGGTTCGGCTTCAACCCCGGCTCGACCATGGCGGCGGACGGCGCGTCCATCGCGCACA
TCGCCACCACCACCAACGTGGCCGCCGCGGCGGGCACCGTCTCCTCCATGCTCGCGGCCTGGATCTTCCTGAAGAAGCCG
GACTTCGGGATCACGCTGAACGGCTGCCTGGCGGGCCTGGTGGCCATCACGGCCGGCTGCGCGTTCGTGAGCGTGCTGAG
CTCGCTCGTCATCGGCCTCGTCGCCGGGGTGCTGGTGGTCGCGGCGGTGGTGTTCTTCGACCGGATCCGCGTGGACGACC
CGGTGGGCGCGACCGCGGTGCACCTCGCGAACGGCGTCTTCGGCACGATCGCGCTCGGCCTGTTCGCGGATCCCACCGTC
GCGCCGTGCGCGGCGGTCGCGAAGCCCGGCCTGTTCCTGGGTGGCGGCATGGCGCAGCTCGGCCCGCAGCTCCTGGGCGT
CGGGGTGGTGGCGGTGTCCGTGTTCACGCTGTCGCTGGCGGCCTGGTACGTGACGAAGCTCCTGTCCGGCGGGATCCGCG
TGACGGCCGAGGAGGAGCTGGAGGGCCTGGACGTCGGCGAGCACGGCAACTCCGCCTACCCGGAGTTCCAGATCCGCTCG
GGCGGCGCGTTCGGCGGCACCGCGTCTCACGACGGCGCGCCGGTCGCGGTCGCGGCGGTCGGCAAGACGACCCCGGCCTA
CTGA

Upstream 100 bases:

>100_bases
ACCGGCCTCGGCGGGTTCCGGTACGCGCGCTGCAGAGGCGCCTCCCGTCCCATCCAACGCGACCGCGTGCGGCCGCCCAC
AGCACGGGAGAACTCCGCCG

Downstream 100 bases:

>100_bases
CCTGGGGCGGGGGGAACGGACGCGAGGCGGGGCCCGTCGCACGGCGGGTCCCGCCTCCGCGCATCCGGGCCGCGGCGCGC
TACCGCGGGGGCAGCGCGGC

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MKKLRALLLLAALAAPAALLLHAPAARADDAAAAAPVAFTQGMADAIAAQKVGLDTIWVMIAAFLVFFMNLGFALVESGF
CRAKNTVNILFKNFVVFAISSLAFLVIGYGLMFGDGNPFFGTSGLLFASGADNSPALGDAYQGVYHALNWTGVPLWAKFF
FQLVFAGTAATIVSGAVAERIKFKAFIVFTLFLVGVVYPVGGHMIWGGGWLASKGFLDFAGSTVVHSIGGWAALAGILVL
GPRLGKYGPGKQINVIPGHNMTSAAIGVFVLWFGWFGFNPGSTMAADGASIAHIATTTNVAAAAGTVSSMLAAWIFLKKP
DFGITLNGCLAGLVAITAGCAFVSVLSSLVIGLVAGVLVVAAVVFFDRIRVDDPVGATAVHLANGVFGTIALGLFADPTV
APCAAVAKPGLFLGGGMAQLGPQLLGVGVVAVSVFTLSLAAWYVTKLLSGGIRVTAEEELEGLDVGEHGNSAYPEFQIRS
GGAFGGTASHDGAPVAVAAVGKTTPAY

Sequences:

>Translated_507_residues
MKKLRALLLLAALAAPAALLLHAPAARADDAAAAAPVAFTQGMADAIAAQKVGLDTIWVMIAAFLVFFMNLGFALVESGF
CRAKNTVNILFKNFVVFAISSLAFLVIGYGLMFGDGNPFFGTSGLLFASGADNSPALGDAYQGVYHALNWTGVPLWAKFF
FQLVFAGTAATIVSGAVAERIKFKAFIVFTLFLVGVVYPVGGHMIWGGGWLASKGFLDFAGSTVVHSIGGWAALAGILVL
GPRLGKYGPGKQINVIPGHNMTSAAIGVFVLWFGWFGFNPGSTMAADGASIAHIATTTNVAAAAGTVSSMLAAWIFLKKP
DFGITLNGCLAGLVAITAGCAFVSVLSSLVIGLVAGVLVVAAVVFFDRIRVDDPVGATAVHLANGVFGTIALGLFADPTV
APCAAVAKPGLFLGGGMAQLGPQLLGVGVVAVSVFTLSLAAWYVTKLLSGGIRVTAEEELEGLDVGEHGNSAYPEFQIRS
GGAFGGTASHDGAPVAVAAVGKTTPAY
>Mature_507_residues
MKKLRALLLLAALAAPAALLLHAPAARADDAAAAAPVAFTQGMADAIAAQKVGLDTIWVMIAAFLVFFMNLGFALVESGF
CRAKNTVNILFKNFVVFAISSLAFLVIGYGLMFGDGNPFFGTSGLLFASGADNSPALGDAYQGVYHALNWTGVPLWAKFF
FQLVFAGTAATIVSGAVAERIKFKAFIVFTLFLVGVVYPVGGHMIWGGGWLASKGFLDFAGSTVVHSIGGWAALAGILVL
GPRLGKYGPGKQINVIPGHNMTSAAIGVFVLWFGWFGFNPGSTMAADGASIAHIATTTNVAAAAGTVSSMLAAWIFLKKP
DFGITLNGCLAGLVAITAGCAFVSVLSSLVIGLVAGVLVVAAVVFFDRIRVDDPVGATAVHLANGVFGTIALGLFADPTV
APCAAVAKPGLFLGGGMAQLGPQLLGVGVVAVSVFTLSLAAWYVTKLLSGGIRVTAEEELEGLDVGEHGNSAYPEFQIRS
GGAFGGTASHDGAPVAVAAVGKTTPAY

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=448, Percent_Identity=35.4910714285714, Blast_Score=205, Evalue=7e-54,
Organism=Caenorhabditis elegans, GI32564042, Length=427, Percent_Identity=33.2552693208431, Blast_Score=209, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI17550180, Length=477, Percent_Identity=33.7526205450734, Blast_Score=201, Evalue=9e-52,
Organism=Caenorhabditis elegans, GI17550182, Length=431, Percent_Identity=34.1067285382831, Blast_Score=197, Evalue=8e-51,
Organism=Caenorhabditis elegans, GI133931100, Length=430, Percent_Identity=29.7674418604651, Blast_Score=167, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6324187, Length=442, Percent_Identity=28.7330316742081, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6325396, Length=428, Percent_Identity=28.2710280373832, Blast_Score=118, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6321559, Length=429, Percent_Identity=27.7389277389277, Blast_Score=113, Evalue=6e-26,
Organism=Drosophila melanogaster, GI161078314, Length=423, Percent_Identity=39.9527186761229, Blast_Score=243, Evalue=2e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 51783; Mature: 51783

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: PS00064 L_LDH ; PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLRALLLLAALAAPAALLLHAPAARADDAAAAAPVAFTQGMADAIAAQKVGLDTIWVM
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCHHHHHHH
IAAFLVFFMNLGFALVESGFCRAKNTVNILFKNFVVFAISSLAFLVIGYGLMFGDGNPFF
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCC
GTSGLLFASGADNSPALGDAYQGVYHALNWTGVPLWAKFFFQLVFAGTAATIVSGAVAER
CCCCEEEECCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IKFKAFIVFTLFLVGVVYPVGGHMIWGGGWLASKGFLDFAGSTVVHSIGGWAALAGILVL
HHHHHHHHHHHHHHHHHHCCCCEEEECCCEECCCCCHHHCCHHHHHHHCHHHHHHHHHHH
GPRLGKYGPGKQINVIPGHNMTSAAIGVFVLWFGWFGFNPGSTMAADGASIAHIATTTNV
CCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCHH
AAAAGTVSSMLAAWIFLKKPDFGITLNGCLAGLVAITAGCAFVSVLSSLVIGLVAGVLVV
HHHHHHHHHHHHHHHHEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAVVFFDRIRVDDPVGATAVHLANGVFGTIALGLFADPTVAPCAAVAKPGLFLGGGMAQL
HHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHCCCEEECCCHHHH
GPQLLGVGVVAVSVFTLSLAAWYVTKLLSGGIRVTAEEELEGLDVGEHGNSAYPEFQIRS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCCCCCCCCCCCCCEEEEC
GGAFGGTASHDGAPVAVAAVGKTTPAY
CCCCCCCCCCCCCCEEEEEECCCCCCC
>Mature Secondary Structure
MKKLRALLLLAALAAPAALLLHAPAARADDAAAAAPVAFTQGMADAIAAQKVGLDTIWVM
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCHHHHHHH
IAAFLVFFMNLGFALVESGFCRAKNTVNILFKNFVVFAISSLAFLVIGYGLMFGDGNPFF
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCC
GTSGLLFASGADNSPALGDAYQGVYHALNWTGVPLWAKFFFQLVFAGTAATIVSGAVAER
CCCCEEEECCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IKFKAFIVFTLFLVGVVYPVGGHMIWGGGWLASKGFLDFAGSTVVHSIGGWAALAGILVL
HHHHHHHHHHHHHHHHHHCCCCEEEECCCEECCCCCHHHCCHHHHHHHCHHHHHHHHHHH
GPRLGKYGPGKQINVIPGHNMTSAAIGVFVLWFGWFGFNPGSTMAADGASIAHIATTTNV
CCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCHH
AAAAGTVSSMLAAWIFLKKPDFGITLNGCLAGLVAITAGCAFVSVLSSLVIGLVAGVLVV
HHHHHHHHHHHHHHHHEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAVVFFDRIRVDDPVGATAVHLANGVFGTIALGLFADPTVAPCAAVAKPGLFLGGGMAQL
HHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHCCCEEECCCHHHH
GPQLLGVGVVAVSVFTLSLAAWYVTKLLSGGIRVTAEEELEGLDVGEHGNSAYPEFQIRS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCCCCCCCCCCCCCEEEEC
GGAFGGTASHDGAPVAVAAVGKTTPAY
CCCCCCCCCCCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]