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The map label for this gene is purH

Identifier: 220916593

GI number: 220916593

Start: 1661654

End: 1663228

Strand: Direct

Name: purH

Synonym: A2cp1_1487

Alternate gene names: 220916593

Gene position: 1661654-1663228 (Clockwise)

Preceding gene: 220916592

Following gene: 220916594

Centisome position: 33.04

GC content: 72.38

Gene sequence:

>1575_bases
ATGACTCGCCGCGCGCTCGTCTCGGTCTCCGACAAGACCGGTCTCGTTCCTTTCGCCAGGCGGCTCGCCGCCCTCGGCGT
GGAGCTGCTCTCCACCGGCGGCACGCAGAAGGCGCTCGCCGAGGCCGGCGTCCCGGTGGTCGGCGTGGGCGACTACACGC
AGGCCCCGGAGATCCTGGGTGGCCGCGTGAAGACGCTCCACCCGCGCGTGCACGGCGGCATCCTCTATCGCCGCGGCCTC
GCCTCCGACGAGGCCGACGTGAAGGCGCGGGACATCCCGCCCATCGACCTCGTGGTGGTGAACCTGTACCCGTTCCGCGA
GGCGGTCGCGGCCGGCAAGCCGTTCGAGACCTGCGTCGAGGAGATCGACATCGGCGGGCCGACCATGGTCCGGAGCGCCG
CCAAGAACTCCGCGCACGTGGGCGTGGTGGTGGACCCGGCCGACTACGAGAAGGTGGCGGCGGAGCTGGAGGCGACGCGC
ACGCTGTCCGCCGCGACGCGCTTCTACCTCATGAAGAAGGCGTTCGCGCACACGGCCGCGTACGACGCCGCCATCTCCGA
GTACCTCACCGCGCGCGAGGCCCCCGAGGCCGCGCCGGCGCACTTCCCGGCCACGCTCGCCGCCGTCTACACGAAGGCGT
ACGATCTCCGGTACGGCGAGAACCCGCACCAGGCCGGCGCGTTCTACCGCGCGGCCCGCGAGCCGGAGGAGCCCTCGGTC
GCGTTCGCCGACGTGCTGCAGGGCAAGGAGCTCAGCTACAACAACCTGCTCGACCTGCAGGCCGCGCTCGCCGGCGTGAT
GGAGTTCGACGAGACCGCCTGCGTGATCATCAAGCACAACACGCCGTGCGGCGTCTCCACCGGCCGCACGGCGGGCGAGG
CGTTCGCGCGCGCCCGCGAGTGCGATCCGGTCTCGGCGTTCGGCGGCATCGTGGCGCTGAACCGGCCCGTGGACGAGGCC
ACCGCCTCGGAGCTCACCAGCCTGTTCCTGGAGTGCGTGATCGCGCCCGGCTACGACGCCGCCGCCCGCGCCGCGCTCGC
GGTGAAGAAGAACCTCCGCCTGCTCGAGGCCCCGCGGCTCGGCGCCGCGCGCGCCACCTGGCGCCGGCGCCCCGAGGAGG
GGCGCGAGCTCCGCTCCATCCCCGGCGGCCTGCTGGTGATGGACCGCGACCTCGGCTCGGTCCGCCGCGACGACTGCAAG
GTGATGACGAAGCGCGCGCCCACCGAGCAGGAGTGGAAGGACCTGCTGTTCGCGTGGAAGGTCGTGAAGCACGTGAAGTC
GAACGCCATCGTGTTCGCGAAGGACGACCGGACCGTGGCGATCGGCGGCGGCCAGACCAGCCGGGTGGAGTCGGTGAAGA
CCGCGGTGATGAAGGCGGCGCTCGACGTCCGCGGCTCCTCGGTGGGCTCCGACGCGTTCTTCCCGTTCGCCGACGGCGTC
GAGGAGATCATCAAGGCCGGCGCCACCGCCATCATCCAGCCCGGCGGCTCGATGCGCGACGCCGAGGTGATCGCCGCGGC
CGACAAGGCCGGCATCGCCATGGTCGCGACCGGCATGCGGCACTTCCGGCACTGA

Upstream 100 bases:

>100_bases
CTGAGCTGGCCGAAGGGGCGGCGGGGCGTCGGGCACCCACGCCCTTGAAAGGTGTCGCCGCGAGCGTTACAAGGGCGCCC
TCCCACGGAGGAAGCCCGAC

Downstream 100 bases:

>100_bases
CCGCCGGGGCGCCTTTCCGGGCGCGGCCGCGCGCCGGCCCGGGCCGGGGAGGCCCGCGGCGCCTGCGCCCCCGCCGCGCG
GGGCCCCCTCCCTGCACGCC

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MTRRALVSVSDKTGLVPFARRLAALGVELLSTGGTQKALAEAGVPVVGVGDYTQAPEILGGRVKTLHPRVHGGILYRRGL
ASDEADVKARDIPPIDLVVVNLYPFREAVAAGKPFETCVEEIDIGGPTMVRSAAKNSAHVGVVVDPADYEKVAAELEATR
TLSAATRFYLMKKAFAHTAAYDAAISEYLTAREAPEAAPAHFPATLAAVYTKAYDLRYGENPHQAGAFYRAAREPEEPSV
AFADVLQGKELSYNNLLDLQAALAGVMEFDETACVIIKHNTPCGVSTGRTAGEAFARARECDPVSAFGGIVALNRPVDEA
TASELTSLFLECVIAPGYDAAARAALAVKKNLRLLEAPRLGAARATWRRRPEEGRELRSIPGGLLVMDRDLGSVRRDDCK
VMTKRAPTEQEWKDLLFAWKVVKHVKSNAIVFAKDDRTVAIGGGQTSRVESVKTAVMKAALDVRGSSVGSDAFFPFADGV
EEIIKAGATAIIQPGGSMRDAEVIAAADKAGIAMVATGMRHFRH

Sequences:

>Translated_524_residues
MTRRALVSVSDKTGLVPFARRLAALGVELLSTGGTQKALAEAGVPVVGVGDYTQAPEILGGRVKTLHPRVHGGILYRRGL
ASDEADVKARDIPPIDLVVVNLYPFREAVAAGKPFETCVEEIDIGGPTMVRSAAKNSAHVGVVVDPADYEKVAAELEATR
TLSAATRFYLMKKAFAHTAAYDAAISEYLTAREAPEAAPAHFPATLAAVYTKAYDLRYGENPHQAGAFYRAAREPEEPSV
AFADVLQGKELSYNNLLDLQAALAGVMEFDETACVIIKHNTPCGVSTGRTAGEAFARARECDPVSAFGGIVALNRPVDEA
TASELTSLFLECVIAPGYDAAARAALAVKKNLRLLEAPRLGAARATWRRRPEEGRELRSIPGGLLVMDRDLGSVRRDDCK
VMTKRAPTEQEWKDLLFAWKVVKHVKSNAIVFAKDDRTVAIGGGQTSRVESVKTAVMKAALDVRGSSVGSDAFFPFADGV
EEIIKAGATAIIQPGGSMRDAEVIAAADKAGIAMVATGMRHFRH
>Mature_523_residues
TRRALVSVSDKTGLVPFARRLAALGVELLSTGGTQKALAEAGVPVVGVGDYTQAPEILGGRVKTLHPRVHGGILYRRGLA
SDEADVKARDIPPIDLVVVNLYPFREAVAAGKPFETCVEEIDIGGPTMVRSAAKNSAHVGVVVDPADYEKVAAELEATRT
LSAATRFYLMKKAFAHTAAYDAAISEYLTAREAPEAAPAHFPATLAAVYTKAYDLRYGENPHQAGAFYRAAREPEEPSVA
FADVLQGKELSYNNLLDLQAALAGVMEFDETACVIIKHNTPCGVSTGRTAGEAFARARECDPVSAFGGIVALNRPVDEAT
ASELTSLFLECVIAPGYDAAARAALAVKKNLRLLEAPRLGAARATWRRRPEEGRELRSIPGGLLVMDRDLGSVRRDDCKV
MTKRAPTEQEWKDLLFAWKVVKHVKSNAIVFAKDDRTVAIGGGQTSRVESVKTAVMKAALDVRGSSVGSDAFFPFADGVE
EIIKAGATAIIQPGGSMRDAEVIAAADKAGIAMVATGMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family

Homologues:

Organism=Homo sapiens, GI20127454, Length=485, Percent_Identity=38.7628865979381, Blast_Score=249, Evalue=3e-66,
Organism=Escherichia coli, GI1790439, Length=534, Percent_Identity=54.1198501872659, Blast_Score=523, Evalue=1e-149,
Organism=Caenorhabditis elegans, GI71985564, Length=484, Percent_Identity=35.1239669421488, Blast_Score=222, Evalue=4e-58,
Organism=Caenorhabditis elegans, GI71985574, Length=316, Percent_Identity=28.1645569620253, Blast_Score=76, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6323768, Length=491, Percent_Identity=36.6598778004073, Blast_Score=247, Evalue=3e-66,
Organism=Saccharomyces cerevisiae, GI6323056, Length=483, Percent_Identity=37.2670807453416, Blast_Score=245, Evalue=1e-65,
Organism=Drosophila melanogaster, GI24649832, Length=500, Percent_Identity=36.2, Blast_Score=225, Evalue=6e-59,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PUR9_ANAD2 (B8JHC9)

Other databases:

- EMBL:   CP001359
- RefSeq:   YP_002491897.1
- ProteinModelPortal:   B8JHC9
- SMR:   B8JHC9
- GeneID:   7296895
- GenomeReviews:   CP001359_GR
- KEGG:   acp:A2cp1_1487
- HOGENOM:   HBG498048
- ProtClustDB:   PRK00881
- HAMAP:   MF_00139
- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607
- Gene3D:   G3DSA:3.40.50.1380
- PANTHER:   PTHR11692
- PIRSF:   PIRSF000414
- SMART:   SM00798
- SMART:   SM00851
- TIGRFAMs:   TIGR00355

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS; SSF53927 Cytidine_deaminase-like; SSF52335 MGS-like_dom

EC number: =2.1.2.3; =3.5.4.10

Molecular weight: Translated: 55939; Mature: 55808

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRRALVSVSDKTGLVPFARRLAALGVELLSTGGTQKALAEAGVPVVGVGDYTQAPEILG
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEECCCCCCCHHHHC
GRVKTLHPRVHGGILYRRGLASDEADVKARDIPPIDLVVVNLYPFREAVAAGKPFETCVE
CCCEEECCHHHCCCCEECCCCCCCCCCCCCCCCCEEEEEEEECCHHHHHHCCCCHHHHHH
EIDIGGPTMVRSAAKNSAHVGVVVDPADYEKVAAELEATRTLSAATRFYLMKKAFAHTAA
HHCCCCHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YDAAISEYLTAREAPEAAPAHFPATLAAVYTKAYDLRYGENPHQAGAFYRAAREPEEPSV
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCH
AFADVLQGKELSYNNLLDLQAALAGVMEFDETACVIIKHNTPCGVSTGRTAGEAFARARE
HHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHC
CDPVSAFGGIVALNRPVDEATASELTSLFLECVIAPGYDAAARAALAVKKNLRLLEAPRL
CCCHHHHCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEECCCC
GAARATWRRRPEEGRELRSIPGGLLVMDRDLGSVRRDDCKVMTKRAPTEQEWKDLLFAWK
CCHHHHHHCCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHH
VVKHVKSNAIVFAKDDRTVAIGGGQTSRVESVKTAVMKAALDVRGSSVGSDAFFPFADGV
HHHHHCCCEEEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
EEIIKAGATAIIQPGGSMRDAEVIAAADKAGIAMVATGMRHFRH
HHHHHCCCCEEECCCCCCCCCHHEEECCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TRRALVSVSDKTGLVPFARRLAALGVELLSTGGTQKALAEAGVPVVGVGDYTQAPEILG
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEECCCCCCCHHHHC
GRVKTLHPRVHGGILYRRGLASDEADVKARDIPPIDLVVVNLYPFREAVAAGKPFETCVE
CCCEEECCHHHCCCCEECCCCCCCCCCCCCCCCCEEEEEEEECCHHHHHHCCCCHHHHHH
EIDIGGPTMVRSAAKNSAHVGVVVDPADYEKVAAELEATRTLSAATRFYLMKKAFAHTAA
HHCCCCHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YDAAISEYLTAREAPEAAPAHFPATLAAVYTKAYDLRYGENPHQAGAFYRAAREPEEPSV
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCH
AFADVLQGKELSYNNLLDLQAALAGVMEFDETACVIIKHNTPCGVSTGRTAGEAFARARE
HHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHC
CDPVSAFGGIVALNRPVDEATASELTSLFLECVIAPGYDAAARAALAVKKNLRLLEAPRL
CCCHHHHCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEECCCC
GAARATWRRRPEEGRELRSIPGGLLVMDRDLGSVRRDDCKVMTKRAPTEQEWKDLLFAWK
CCHHHHHHCCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHH
VVKHVKSNAIVFAKDDRTVAIGGGQTSRVESVKTAVMKAALDVRGSSVGSDAFFPFADGV
HHHHHCCCEEEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
EEIIKAGATAIIQPGGSMRDAEVIAAADKAGIAMVATGMRHFRH
HHHHHCCCCEEECCCCCCCCCHHEEECCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA