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The map label for this gene is aspS

Identifier: 220916524

GI number: 220916524

Start: 1584965

End: 1586776

Strand: Direct

Name: aspS

Synonym: A2cp1_1418

Alternate gene names: 220916524

Gene position: 1584965-1586776 (Clockwise)

Preceding gene: 220916521

Following gene: 220916525

Centisome position: 31.51

GC content: 68.27

Gene sequence:

>1812_bases
TTGCCGCGCTTCATCACCGAGCTCAAGCGTACCCACTCCTGCGGTGAACTCACCAAGGCCGACATCGGCAAGGAGGTCGT
GCTGTTCGGCTGGGTGAACAACCGGCGGGACCACGGCGGCGCGGTGTTCATCGACCTGCGCGACCGCGCCGGCCTGACCC
AGGTGGTGTTCGAGGAGGACGTCCGCCCCGACGTGCACGAGCTCGCCGGGCAGCTCCGGCTCGAGTACTGCGTCGGCGTG
CGCGGCAAGGTGGTCTCGCGCGGCGGCAACGTGAACCCGAAGCTCCGCACCGGCGAGATCGAGGTCCACGCGTCGGACCT
CGAGATCTTCAACCGCTCCGAGCCGGCGCCGTTCCAGATCGAGGACGAGATCGACACCGGCGAGGAGAAGCGGCTCCAGT
ACCGCTACCTCGACCTGCGCCGCGCCCCGCTGCAGCGGACGCTCATGACCCGCGCGAAGGTGAACCACCTCACCCGCAAC
TACTTCACCGACAAGGGCTTCCTCGAGCTCGAGACGCCGTTCATGGTGAAGTACACCCCGGGCGGCGCCCGCAACTTCCT
CGTCCCCTCGCGCCTCAACCCCGGCAAGTTCTACGCGCTCGCCGAGAGCCCGCAGCTGTTCAAGCAGCTCTACATGATGG
CGGGCTTCGACCGGTACTTCCAGATCGTCCGCTGCTTCCGCGACGAGGACCTCCGGCTCGACCGGCAGCCCGAGTTCACG
CAGATCGACGTCGAGATGTCGTTCGTGGAGCAGAACGACGTGTTCGACGTGATGGAGGGGCTGGTGGTGAAGCTCTGGAA
GGAGGTGCTCGGGATCGAGATCCCGCGCCCGTTCCAGCGCATGCCGTTCGAGGAGTCGATGGCGAAGTACGGGAACGACA
AGCCCGACCTCCGCTTCGACATGCCGCACGTGGTGCTCACCGACCTGGTGCGCCAGCACGACGGCGGCGGCGTCCCGCTC
ATGCACGAGGCGGTGAAGGCCAAGGGCATCGTGAAGGCGATGCGCGTGCCCGCCTCGGCGAACTTCTCGCGCACCGAGAT
CGACAAGCTCGAGGAGTACGTGAAGGGCATGGGCGCGAAGGGGCTCGCCCGCGCCAAGGTCGGCGAGGGCGGCGAGTGGA
CCCAGTCGCCGCTCGCGAAGACGATCACCCCCGCGCTGCGCCAGGCGATCAACGAGGCCTGCGAGGCGAAGCCCGGCGAC
CTGCTCCTGTTCCAGTTCGGCAAGGAGTCGGTGGTCCACACGGTGATGGCGAACCTGCGCGTGCACCTCGCCAAGCGCAT
GGGCCTCATCCCCGAGTACGGGTCGGGCGGCGCCTGGCGCTTCCTGTGGGTGGTGAACCCGCCGCTGTTCGAGTACGACG
AGGAGTCCGGCCAGTGGGCCGCCGCGCACCACGCGTTCACGCGGCCGCACGACTCCGATCTCCAGTTCCTCGAGAGCGAT
CCGGGCAAGGTGAACTGCTACCGGTACGACCTGGTGCTGAACGGGTTCGAGATCGGCGGCGGATCCATCCGCCTCCACGA
CCCCGAGGTCCAGGCCCGGGTGTTCAAGGCCATGGGCATCACCGACGAGGAGGCCCGCAGCAAGTTCGGCTTCCTGCTCG
ACGCGCTCAAGATGGGCGCGCCGCCGCACGGCGGCATCGCGCTCGGCATGGACCGCCTGGTCATGCTCCTCACCGGCGCC
GAGTCGCTCCGCGACGTGGTGGCCTGGCCCAAGACGCAGAAGGGCACCGACCTCATGACCGGCGCCCCCGGCGACGTGGA
CGCGCGCCAGCTCCGCGAGCTGTACGTGAAGAGCACGTACGAGCCGAAGTAG

Upstream 100 bases:

>100_bases
GAGACCCGGCGGCGGCGCGCACCGGAAGGGCTGCGGTCGTCCTTGACGCCTGAGGTTCCGCAGTGTTAGCTGCCTTTCCC
CACGTCCGGAGGCCCTTCCC

Downstream 100 bases:

>100_bases
CGGCGCCGCGCCTGCCCGCGGCGCGCCGCCGCGGCCCCGCGCCCGGAGGCGCCTCACCTTTCGCGCGACCGCGCCCCCGG
TCCGGGCTACGCTTCGGAGC

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 603; Mature: 602

Protein sequence:

>603_residues
MPRFITELKRTHSCGELTKADIGKEVVLFGWVNNRRDHGGAVFIDLRDRAGLTQVVFEEDVRPDVHELAGQLRLEYCVGV
RGKVVSRGGNVNPKLRTGEIEVHASDLEIFNRSEPAPFQIEDEIDTGEEKRLQYRYLDLRRAPLQRTLMTRAKVNHLTRN
YFTDKGFLELETPFMVKYTPGGARNFLVPSRLNPGKFYALAESPQLFKQLYMMAGFDRYFQIVRCFRDEDLRLDRQPEFT
QIDVEMSFVEQNDVFDVMEGLVVKLWKEVLGIEIPRPFQRMPFEESMAKYGNDKPDLRFDMPHVVLTDLVRQHDGGGVPL
MHEAVKAKGIVKAMRVPASANFSRTEIDKLEEYVKGMGAKGLARAKVGEGGEWTQSPLAKTITPALRQAINEACEAKPGD
LLLFQFGKESVVHTVMANLRVHLAKRMGLIPEYGSGGAWRFLWVVNPPLFEYDEESGQWAAAHHAFTRPHDSDLQFLESD
PGKVNCYRYDLVLNGFEIGGGSIRLHDPEVQARVFKAMGITDEEARSKFGFLLDALKMGAPPHGGIALGMDRLVMLLTGA
ESLRDVVAWPKTQKGTDLMTGAPGDVDARQLRELYVKSTYEPK

Sequences:

>Translated_603_residues
MPRFITELKRTHSCGELTKADIGKEVVLFGWVNNRRDHGGAVFIDLRDRAGLTQVVFEEDVRPDVHELAGQLRLEYCVGV
RGKVVSRGGNVNPKLRTGEIEVHASDLEIFNRSEPAPFQIEDEIDTGEEKRLQYRYLDLRRAPLQRTLMTRAKVNHLTRN
YFTDKGFLELETPFMVKYTPGGARNFLVPSRLNPGKFYALAESPQLFKQLYMMAGFDRYFQIVRCFRDEDLRLDRQPEFT
QIDVEMSFVEQNDVFDVMEGLVVKLWKEVLGIEIPRPFQRMPFEESMAKYGNDKPDLRFDMPHVVLTDLVRQHDGGGVPL
MHEAVKAKGIVKAMRVPASANFSRTEIDKLEEYVKGMGAKGLARAKVGEGGEWTQSPLAKTITPALRQAINEACEAKPGD
LLLFQFGKESVVHTVMANLRVHLAKRMGLIPEYGSGGAWRFLWVVNPPLFEYDEESGQWAAAHHAFTRPHDSDLQFLESD
PGKVNCYRYDLVLNGFEIGGGSIRLHDPEVQARVFKAMGITDEEARSKFGFLLDALKMGAPPHGGIALGMDRLVMLLTGA
ESLRDVVAWPKTQKGTDLMTGAPGDVDARQLRELYVKSTYEPK
>Mature_602_residues
PRFITELKRTHSCGELTKADIGKEVVLFGWVNNRRDHGGAVFIDLRDRAGLTQVVFEEDVRPDVHELAGQLRLEYCVGVR
GKVVSRGGNVNPKLRTGEIEVHASDLEIFNRSEPAPFQIEDEIDTGEEKRLQYRYLDLRRAPLQRTLMTRAKVNHLTRNY
FTDKGFLELETPFMVKYTPGGARNFLVPSRLNPGKFYALAESPQLFKQLYMMAGFDRYFQIVRCFRDEDLRLDRQPEFTQ
IDVEMSFVEQNDVFDVMEGLVVKLWKEVLGIEIPRPFQRMPFEESMAKYGNDKPDLRFDMPHVVLTDLVRQHDGGGVPLM
HEAVKAKGIVKAMRVPASANFSRTEIDKLEEYVKGMGAKGLARAKVGEGGEWTQSPLAKTITPALRQAINEACEAKPGDL
LLFQFGKESVVHTVMANLRVHLAKRMGLIPEYGSGGAWRFLWVVNPPLFEYDEESGQWAAAHHAFTRPHDSDLQFLESDP
GKVNCYRYDLVLNGFEIGGGSIRLHDPEVQARVFKAMGITDEEARSKFGFLLDALKMGAPPHGGIALGMDRLVMLLTGAE
SLRDVVAWPKTQKGTDLMTGAPGDVDARQLRELYVKSTYEPK

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=609, Percent_Identity=40.3940886699507, Blast_Score=428, Evalue=1e-120,
Organism=Homo sapiens, GI45439306, Length=295, Percent_Identity=27.7966101694915, Blast_Score=90, Evalue=8e-18,
Organism=Homo sapiens, GI5031815, Length=255, Percent_Identity=27.4509803921569, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI194272210, Length=255, Percent_Identity=27.4509803921569, Blast_Score=81, Evalue=3e-15,
Organism=Escherichia coli, GI1788173, Length=592, Percent_Identity=48.1418918918919, Blast_Score=529, Evalue=1e-151,
Organism=Escherichia coli, GI1789256, Length=313, Percent_Identity=27.7955271565495, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1790571, Length=313, Percent_Identity=27.7955271565495, Blast_Score=112, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI32566633, Length=293, Percent_Identity=41.296928327645, Blast_Score=225, Evalue=6e-59,
Organism=Caenorhabditis elegans, GI71994340, Length=306, Percent_Identity=24.1830065359477, Blast_Score=79, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17535927, Length=262, Percent_Identity=25.1908396946565, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17535925, Length=262, Percent_Identity=25.1908396946565, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17551876, Length=315, Percent_Identity=24.7619047619048, Blast_Score=75, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6325153, Length=315, Percent_Identity=36.5079365079365, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6323011, Length=289, Percent_Identity=30.7958477508651, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6321807, Length=264, Percent_Identity=25.3787878787879, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320242, Length=203, Percent_Identity=27.5862068965517, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24584738, Length=596, Percent_Identity=36.744966442953, Blast_Score=356, Evalue=3e-98,
Organism=Drosophila melanogaster, GI17136276, Length=275, Percent_Identity=26.1818181818182, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24640851, Length=247, Percent_Identity=25.5060728744939, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24640849, Length=248, Percent_Identity=25.8064516129032, Blast_Score=79, Evalue=1e-14,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_ANAD2 (B8JH60)

Other databases:

- EMBL:   CP001359
- RefSeq:   YP_002491828.1
- GeneID:   7299939
- GenomeReviews:   CP001359_GR
- KEGG:   acp:A2cp1_1418
- HOGENOM:   HBG396032
- ProtClustDB:   PRK00476
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 68149; Mature: 68018

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRFITELKRTHSCGELTKADIGKEVVLFGWVNNRRDHGGAVFIDLRDRAGLTQVVFEED
CCHHHHHHHHHCCCCCHHHHCCCCEEEEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHC
VRPDVHELAGQLRLEYCVGVRGKVVSRGGNVNPKLRTGEIEVHASDLEIFNRSEPAPFQI
CCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEECEEEEEECCHHHHCCCCCCCEEE
EDEIDTGEEKRLQYRYLDLRRAPLQRTLMTRAKVNHLTRNYFTDKGFLELETPFMVKYTP
CCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEECC
GGARNFLVPSRLNPGKFYALAESPQLFKQLYMMAGFDRYFQIVRCFRDEDLRLDRQPEFT
CCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCE
QIDVEMSFVEQNDVFDVMEGLVVKLWKEVLGIEIPRPFQRMPFEESMAKYGNDKPDLRFD
EEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHCCCCCCEEEC
MPHVVLTDLVRQHDGGGVPLMHEAVKAKGIVKAMRVPASANFSRTEIDKLEEYVKGMGAK
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCC
GLARAKVGEGGEWTQSPLAKTITPALRQAINEACEAKPGDLLLFQFGKESVVHTVMANLR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHH
VHLAKRMGLIPEYGSGGAWRFLWVVNPPLFEYDEESGQWAAAHHAFTRPHDSDLQFLESD
HHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEHHHHCCCCCCCCCCHHHCCC
PGKVNCYRYDLVLNGFEIGGGSIRLHDPEVQARVFKAMGITDEEARSKFGFLLDALKMGA
CCEEEEEEEEEEEEEEEECCCEEEEECHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
PPHGGIALGMDRLVMLLTGAESLRDVVAWPKTQKGTDLMTGAPGDVDARQLRELYVKSTY
CCCCCEEHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
EPK
CCC
>Mature Secondary Structure 
PRFITELKRTHSCGELTKADIGKEVVLFGWVNNRRDHGGAVFIDLRDRAGLTQVVFEED
CHHHHHHHHHCCCCCHHHHCCCCEEEEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHC
VRPDVHELAGQLRLEYCVGVRGKVVSRGGNVNPKLRTGEIEVHASDLEIFNRSEPAPFQI
CCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEECEEEEEECCHHHHCCCCCCCEEE
EDEIDTGEEKRLQYRYLDLRRAPLQRTLMTRAKVNHLTRNYFTDKGFLELETPFMVKYTP
CCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEECC
GGARNFLVPSRLNPGKFYALAESPQLFKQLYMMAGFDRYFQIVRCFRDEDLRLDRQPEFT
CCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCE
QIDVEMSFVEQNDVFDVMEGLVVKLWKEVLGIEIPRPFQRMPFEESMAKYGNDKPDLRFD
EEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHCCCCCCEEEC
MPHVVLTDLVRQHDGGGVPLMHEAVKAKGIVKAMRVPASANFSRTEIDKLEEYVKGMGAK
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCC
GLARAKVGEGGEWTQSPLAKTITPALRQAINEACEAKPGDLLLFQFGKESVVHTVMANLR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHH
VHLAKRMGLIPEYGSGGAWRFLWVVNPPLFEYDEESGQWAAAHHAFTRPHDSDLQFLESD
HHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEHHHHCCCCCCCCCCHHHCCC
PGKVNCYRYDLVLNGFEIGGGSIRLHDPEVQARVFKAMGITDEEARSKFGFLLDALKMGA
CCEEEEEEEEEEEEEEEECCCEEEEECHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
PPHGGIALGMDRLVMLLTGAESLRDVVAWPKTQKGTDLMTGAPGDVDARQLRELYVKSTY
CCCCCEEHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
EPK
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA