Click here to switch to the map view.

The map label for this gene is truB [H]

Identifier: 220916339

GI number: 220916339

Start: 1383977

End: 1384918

Strand: Direct

Name: truB [H]

Synonym: A2cp1_1233

Alternate gene names: 220916339

Gene position: 1383977-1384918 (Clockwise)

Preceding gene: 220916338

Following gene: 220916340

Centisome position: 27.52

GC content: 79.83

Gene sequence:

>942_bases
GTGCGCCCGGGCGTGCTCGTGGTGGACAAGCCCGCCGGCCCCACCAGCTTCGAGGTGGTGCGCCGGATCCGGCGCGCGCT
CGGCGCGGCGCGGGCGGGCCACGCCGGCACGCTCGACCCGGCCGCCACCGGCGTGCTGGCGATCTGCGTCGAGGACGGCG
TGAAGCTGCAGCAGTTCCTGTCCGACGGCGACAAGGCCTACGACGCCGCCGTGGCGTTCGGCGCGGCCACGAGCACCGAG
GACGCGGACGGCGAGGTGACCGCGCGCGGCGACGCCTCGGCGCTGACCGAGGACGCCCTGCGCGCCGCGCTCGCCGGGCT
CACCGGCGACATCGAGCAGGTCCCCCCGATGTACTCGGCGGTCCGGGTGGGCGGGCGGCGCCTGCACGAGGTGGCCCGCG
CCGGGGAGACGGTGGACCGCACGCCGCGGCGGGTGCGCGTGCACGCGCTCGAGCTGGGCGGGCTCGACGCCGCGCCCGGG
CCGGACGGCCTGCGGCGCGCCCGGGTGGCGGTGCGCTGCGGGAAGGGCACCTACGTCCGCACGCTCGCGGCCGACCTGGG
CCGGGCGCTCGGCGTTCCCGCGCACCTCGCGGCCCTGCGCCGCACCATGGCGAGCGGGTTCGACCTCTCGCGCGCCATCG
GCCTGGAGGACGCGGAGGCGCTCGCGCGCGACCACGGGCGCGAGGCGCTGGCGGAGCGGATCGTTCCCATGGCGGACGCG
CTGGGCGCGCTCGGCGCCATCCGGCTGGGAGAGCGCGAGGCGTGGGATCTCGTGCACGGGCGCCCGGTGCCCCGGCCGCC
CGGGGCCCCCGGCGTGGTGCGCGCGCTGGCGCCGGACGGCCGCCTGGTCGCCGTGTGCGCGCCGGGCGAGGGGCGCCTGC
GGACCCTGCGCGTGTTCCTGGGCCCCTCCGACCTCCGGGCGGGCCCCGCCCAGAATCGTTGA

Upstream 100 bases:

>100_bases
CTGCGCGAGGCGCGCACGCAGGGGCAGGCGGCGCCGGCCCCGGACGTCGAGCCCGCCCCGGGCGCGGCGCCGGACGACGA
GGCAGAGGAGTAGCCGCCGC

Downstream 100 bases:

>100_bases
CTCCCCCGCCGCGATCGCTTAAGACCGCGCATTCACGACGCTCACTGCGGCCCGAGGCGCGCGCCTTCGCCCCCGCGGCG
GTGAGCGATACAGACGAAAG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MRPGVLVVDKPAGPTSFEVVRRIRRALGAARAGHAGTLDPAATGVLAICVEDGVKLQQFLSDGDKAYDAAVAFGAATSTE
DADGEVTARGDASALTEDALRAALAGLTGDIEQVPPMYSAVRVGGRRLHEVARAGETVDRTPRRVRVHALELGGLDAAPG
PDGLRRARVAVRCGKGTYVRTLAADLGRALGVPAHLAALRRTMASGFDLSRAIGLEDAEALARDHGREALAERIVPMADA
LGALGAIRLGEREAWDLVHGRPVPRPPGAPGVVRALAPDGRLVAVCAPGEGRLRTLRVFLGPSDLRAGPAQNR

Sequences:

>Translated_313_residues
MRPGVLVVDKPAGPTSFEVVRRIRRALGAARAGHAGTLDPAATGVLAICVEDGVKLQQFLSDGDKAYDAAVAFGAATSTE
DADGEVTARGDASALTEDALRAALAGLTGDIEQVPPMYSAVRVGGRRLHEVARAGETVDRTPRRVRVHALELGGLDAAPG
PDGLRRARVAVRCGKGTYVRTLAADLGRALGVPAHLAALRRTMASGFDLSRAIGLEDAEALARDHGREALAERIVPMADA
LGALGAIRLGEREAWDLVHGRPVPRPPGAPGVVRALAPDGRLVAVCAPGEGRLRTLRVFLGPSDLRAGPAQNR
>Mature_313_residues
MRPGVLVVDKPAGPTSFEVVRRIRRALGAARAGHAGTLDPAATGVLAICVEDGVKLQQFLSDGDKAYDAAVAFGAATSTE
DADGEVTARGDASALTEDALRAALAGLTGDIEQVPPMYSAVRVGGRRLHEVARAGETVDRTPRRVRVHALELGGLDAAPG
PDGLRRARVAVRCGKGTYVRTLAADLGRALGVPAHLAALRRTMASGFDLSRAIGLEDAEALARDHGREALAERIVPMADA
LGALGAIRLGEREAWDLVHGRPVPRPPGAPGVVRALAPDGRLVAVCAPGEGRLRTLRVFLGPSDLRAGPAQNR

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=223, Percent_Identity=34.9775784753363, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI215599015, Length=301, Percent_Identity=27.2425249169435, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI4503337, Length=301, Percent_Identity=27.2425249169435, Blast_Score=82, Evalue=4e-16,
Organism=Escherichia coli, GI2367200, Length=280, Percent_Identity=35.7142857142857, Blast_Score=162, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6324037, Length=135, Percent_Identity=35.5555555555556, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6323204, Length=201, Percent_Identity=30.8457711442786, Blast_Score=87, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240 [H]

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 32520; Mature: 32520

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS00687 ALDEHYDE_DEHYDR_GLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPGVLVVDKPAGPTSFEVVRRIRRALGAARAGHAGTLDPAATGVLAICVEDGVKLQQFL
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHCEEEEEHHCCHHHHHHH
SDGDKAYDAAVAFGAATSTEDADGEVTARGDASALTEDALRAALAGLTGDIEQVPPMYSA
CCCCHHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCHHHCCHHHHH
VRVGGRRLHEVARAGETVDRTPRRVRVHALELGGLDAAPGPDGLRRARVAVRCGKGTYVR
HHHHHHHHHHHHHCCCCHHCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHEEECCCHHHH
TLAADLGRALGVPAHLAALRRTMASGFDLSRAIGLEDAEALARDHGREALAERIVPMADA
HHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHH
LGALGAIRLGEREAWDLVHGRPVPRPPGAPGVVRALAPDGRLVAVCAPGEGRLRTLRVFL
HHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCEEEECCCCCEEEEECCCCCCEEEEEEEE
GPSDLRAGPAQNR
CCHHCCCCCCCCC
>Mature Secondary Structure
MRPGVLVVDKPAGPTSFEVVRRIRRALGAARAGHAGTLDPAATGVLAICVEDGVKLQQFL
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHCEEEEEHHCCHHHHHHH
SDGDKAYDAAVAFGAATSTEDADGEVTARGDASALTEDALRAALAGLTGDIEQVPPMYSA
CCCCHHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCHHHCCHHHHH
VRVGGRRLHEVARAGETVDRTPRRVRVHALELGGLDAAPGPDGLRRARVAVRCGKGTYVR
HHHHHHHHHHHHHCCCCHHCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHEEECCCHHHH
TLAADLGRALGVPAHLAALRRTMASGFDLSRAIGLEDAEALARDHGREALAERIVPMADA
HHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHH
LGALGAIRLGEREAWDLVHGRPVPRPPGAPGVVRALAPDGRLVAVCAPGEGRLRTLRVFL
HHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCEEEECCCCCEEEEECCCCCCEEEEEEEE
GPSDLRAGPAQNR
CCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA