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The map label for this gene is traD [H]

Identifier: 220916219

GI number: 220916219

Start: 1245654

End: 1247183

Strand: Reverse

Name: traD [H]

Synonym: A2cp1_1111

Alternate gene names: 220916219

Gene position: 1247183-1245654 (Counterclockwise)

Preceding gene: 220916220

Following gene: 220916218

Centisome position: 24.8

GC content: 70.46

Gene sequence:

>1530_bases
ATGCACCCCGACGATCGGATGAAGGCGGGCGCCTTCCTCGTGGCCGGGCTGCCGCTCGCGGCGTGGATCGCGGCGGCGTG
GGCGACCGCCGAGATCGAGCACCCGGGCCTGAGCCGGCTCCTCCACCTCGCGACGCTCACGGCCACGGGAGCGCGGCCGC
TCCTTCTCGTCGCGGCGTTGGGCGGTCTCGCCCTCGCCGTCGTGGTCATCGTCGTAGCGCGCAGGCTGGGGGCGGCCGGC
TTCGGCGGGGCTGCGTTCCGGCTCCACCTCCGCGGCACGAGGGTAGTGAGCGCGATGCGCCTCGCTTGGTCCACCCGCGA
GCGCAGGCGCCAGCAGATCACCGTCGCGGGCGTACCGATGCCGACGTCGGTAGAGACCCTCCACCTCCTCGTCGGCGGGT
CGACTGGGTCCGGCAAGTCGGTCCTCATCCGCGAGATGGCCTACTCGGCCCTCCTCCGCGGCGATCGCATCGTGGTAGCC
GATCCGAACGGCGACATGCTCGCGAAGTTCTACCGGCCTGGCGACGTCATCTTGAACCCCTACGACTCGCGCGGTCTCGG
CTGGACCTTCTTCAACGAGATCAGGGCCGAGTACGACTTCAAGCGCTTCGCCCTGTCGCTCGTCCCGCGAGGCCAGACGA
AGGAGGAGGAGGAGTGGTGCTCCTACGCTCGGCTCCTCCTGCGCGAGACCGCCCGGAAGCTCTCGCTCGTGGGCCAGCCG
TCGGTGGCCGAGCTGTTCCGCTGGACGACCATCGCGAAGCCGGCGGACCTCCAGGCCTTCCTCGCCCAGACGGCGGCCGA
GTCGCTCTTCGTCGGAGCCGACAAGGCACTCGCGAGCGCCCGCTTCGTGCTCTCGGCGAAACTCCCAGAGCATCTCTCCA
TGCCAGCCGGCGACTTCTCGCTGCGTTCCTGGCTCGCCGATCCCGCCGGCGGGAACCTCTTCATCACGTGGCGCGAGGAC
ATGGCCGAGGCGCTGAAGCCGCTCATCTCGGCCTGGGTCGACGTGCTGTGCACCTCCATCCTGTCGCTCGGCGAGGACCG
GGAGCGGCGCGTCTGGATGTTCCTCGACGAGCTTGCGAGCCTGGAGAAGCTGCCGAGCTTGGAGGACGCCGCGACCAAGG
GCCGCAAGGCCGGGCTCCGGATCGTGGCCGGCCTCCAGTCGACCGCCCAGCTCGAGCGGATCTACGGGCGCGAGGAGGCC
CAGACGCTCCGGAGCTGCTTCCGGTCGCTCGTCGTCCTCGGCGGCGCGAAGACAGATCCCCGGACCTGCGAGGACATGAG
CCAGAGCCTCGGCGAGCACGAGGTGGAGCGCGAGAGCTTCAGCAGGACGACCGGGCTCCGGGGCGACTCGACTAGCAGCC
AGCTCCAGCGCGCGCGGGAGCGCGTCGTGCTCCCGTCCGAGATCGCGAGCCTCCCCGACCTGACCGGCTACCTCGCGTTC
GCCGGGAACCACCCCATCGCGAAGGTGAAGCTCGACGTGGTCCGTTTCCGGAACCGCGTCCCGGCCTTCGAGGAGAGGCT
GGCATGCTGA

Upstream 100 bases:

>100_bases
CGAGGTGCTGCTCTTGCTCCGCTCGCTCGCGGGTCCGCAGAAGTCGGCCGTCGCCCAGAAGGAAGTGGAGCGGCGCGGCC
TCTCGTCCTGGAGATAGCCC

Downstream 100 bases:

>100_bases
GCCACAAGGTGCTCACGCGACAGGACGTAGGCCGCGCCGCGAGCTACTACGAGGACGGCGCCGACGACTACTACGCCGGC
GAGGGCGACTCCTCCGCGTG

Product: type IV secretion system protein VirD4

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MHPDDRMKAGAFLVAGLPLAAWIAAAWATAEIEHPGLSRLLHLATLTATGARPLLLVAALGGLALAVVVIVVARRLGAAG
FGGAAFRLHLRGTRVVSAMRLAWSTRERRRQQITVAGVPMPTSVETLHLLVGGSTGSGKSVLIREMAYSALLRGDRIVVA
DPNGDMLAKFYRPGDVILNPYDSRGLGWTFFNEIRAEYDFKRFALSLVPRGQTKEEEEWCSYARLLLRETARKLSLVGQP
SVAELFRWTTIAKPADLQAFLAQTAAESLFVGADKALASARFVLSAKLPEHLSMPAGDFSLRSWLADPAGGNLFITWRED
MAEALKPLISAWVDVLCTSILSLGEDRERRVWMFLDELASLEKLPSLEDAATKGRKAGLRIVAGLQSTAQLERIYGREEA
QTLRSCFRSLVVLGGAKTDPRTCEDMSQSLGEHEVERESFSRTTGLRGDSTSSQLQRARERVVLPSEIASLPDLTGYLAF
AGNHPIAKVKLDVVRFRNRVPAFEERLAC

Sequences:

>Translated_509_residues
MHPDDRMKAGAFLVAGLPLAAWIAAAWATAEIEHPGLSRLLHLATLTATGARPLLLVAALGGLALAVVVIVVARRLGAAG
FGGAAFRLHLRGTRVVSAMRLAWSTRERRRQQITVAGVPMPTSVETLHLLVGGSTGSGKSVLIREMAYSALLRGDRIVVA
DPNGDMLAKFYRPGDVILNPYDSRGLGWTFFNEIRAEYDFKRFALSLVPRGQTKEEEEWCSYARLLLRETARKLSLVGQP
SVAELFRWTTIAKPADLQAFLAQTAAESLFVGADKALASARFVLSAKLPEHLSMPAGDFSLRSWLADPAGGNLFITWRED
MAEALKPLISAWVDVLCTSILSLGEDRERRVWMFLDELASLEKLPSLEDAATKGRKAGLRIVAGLQSTAQLERIYGREEA
QTLRSCFRSLVVLGGAKTDPRTCEDMSQSLGEHEVERESFSRTTGLRGDSTSSQLQRARERVVLPSEIASLPDLTGYLAF
AGNHPIAKVKLDVVRFRNRVPAFEERLAC
>Mature_509_residues
MHPDDRMKAGAFLVAGLPLAAWIAAAWATAEIEHPGLSRLLHLATLTATGARPLLLVAALGGLALAVVVIVVARRLGAAG
FGGAAFRLHLRGTRVVSAMRLAWSTRERRRQQITVAGVPMPTSVETLHLLVGGSTGSGKSVLIREMAYSALLRGDRIVVA
DPNGDMLAKFYRPGDVILNPYDSRGLGWTFFNEIRAEYDFKRFALSLVPRGQTKEEEEWCSYARLLLRETARKLSLVGQP
SVAELFRWTTIAKPADLQAFLAQTAAESLFVGADKALASARFVLSAKLPEHLSMPAGDFSLRSWLADPAGGNLFITWRED
MAEALKPLISAWVDVLCTSILSLGEDRERRVWMFLDELASLEKLPSLEDAATKGRKAGLRIVAGLQSTAQLERIYGREEA
QTLRSCFRSLVVLGGAKTDPRTCEDMSQSLGEHEVERESFSRTTGLRGDSTSSQLQRARERVVLPSEIASLPDLTGYLAF
AGNHPIAKVKLDVVRFRNRVPAFEERLAC

Specific function: TraD is a cell envelope protein. The function of traD is unknown but it is essential for DNA transfer. It may form or may be part of a non-specific pore for DNA export, or it may directly energizes DNA transport [H]

COG id: COG3505

COG function: function code U; Type IV secretory pathway, VirD4 components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To traD of plasmid F [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014128
- InterPro:   IPR019476
- InterPro:   IPR022585 [H]

Pfam domain/function: PF12615 TraD_N; PF10412 TrwB_AAD_bind [H]

EC number: NA

Molecular weight: Translated: 55702; Mature: 55702

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPDDRMKAGAFLVAGLPLAAWIAAAWATAEIEHPGLSRLLHLATLTATGARPLLLVAAL
CCCCCHHCCCCCEEECHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHH
GGLALAVVVIVVARRLGAAGFGGAAFRLHLRGTRVVSAMRLAWSTRERRRQQITVAGVPM
HHHHHHHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHEEEEECCCC
PTSVETLHLLVGGSTGSGKSVLIREMAYSALLRGDRIVVADPNGDMLAKFYRPGDVILNP
CCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCEEHEEECCCCEEECC
YDSRGLGWTFFNEIRAEYDFKRFALSLVPRGQTKEEEEWCSYARLLLRETARKLSLVGQP
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
SVAELFRWTTIAKPADLQAFLAQTAAESLFVGADKALASARFVLSAKLPEHLSMPAGDFS
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHCCCCCCHH
LRSWLADPAGGNLFITWREDMAEALKPLISAWVDVLCTSILSLGEDRERRVWMFLDELAS
HHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LEKLPSLEDAATKGRKAGLRIVAGLQSTAQLERIYGREEAQTLRSCFRSLVVLGGAKTDP
HHHCCCHHHHHHCCHHCCHHHEECCHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCC
RTCEDMSQSLGEHEVERESFSRTTGLRGDSTSSQLQRARERVVLPSEIASLPDLTGYLAF
CCHHHHHHHHCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHCCCCHHHHEEE
AGNHPIAKVKLDVVRFRNRVPAFEERLAC
CCCCCEEEHHHHHHHHHHCCCCHHHHCCC
>Mature Secondary Structure
MHPDDRMKAGAFLVAGLPLAAWIAAAWATAEIEHPGLSRLLHLATLTATGARPLLLVAAL
CCCCCHHCCCCCEEECHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHH
GGLALAVVVIVVARRLGAAGFGGAAFRLHLRGTRVVSAMRLAWSTRERRRQQITVAGVPM
HHHHHHHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHEEEEECCCC
PTSVETLHLLVGGSTGSGKSVLIREMAYSALLRGDRIVVADPNGDMLAKFYRPGDVILNP
CCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCEEHEEECCCCEEECC
YDSRGLGWTFFNEIRAEYDFKRFALSLVPRGQTKEEEEWCSYARLLLRETARKLSLVGQP
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
SVAELFRWTTIAKPADLQAFLAQTAAESLFVGADKALASARFVLSAKLPEHLSMPAGDFS
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHCCCCCCHH
LRSWLADPAGGNLFITWREDMAEALKPLISAWVDVLCTSILSLGEDRERRVWMFLDELAS
HHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LEKLPSLEDAATKGRKAGLRIVAGLQSTAQLERIYGREEAQTLRSCFRSLVVLGGAKTDP
HHHCCCHHHHHHCCHHCCHHHEECCHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCC
RTCEDMSQSLGEHEVERESFSRTTGLRGDSTSSQLQRARERVVLPSEIASLPDLTGYLAF
CCHHHHHHHHCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHCCCCHHHHEEE
AGNHPIAKVKLDVVRFRNRVPAFEERLAC
CCCCCEEEHHHHHHHHHHCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2164585 [H]