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The map label for this gene is traI [H]

Identifier: 220916218

GI number: 220916218

Start: 1242880

End: 1245660

Strand: Reverse

Name: traI [H]

Synonym: A2cp1_1110

Alternate gene names: 220916218

Gene position: 1245660-1242880 (Counterclockwise)

Preceding gene: 220916219

Following gene: 220916217

Centisome position: 24.77

GC content: 70.55

Gene sequence:

>2781_bases
ATGCTGAGCCACAAGGTGCTCACGCGACAGGACGTAGGCCGCGCCGCGAGCTACTACGAGGACGGCGCCGACGACTACTA
CGCCGGCGAGGGCGACTCCTCCGCGTGGCAGGGGAAGGGCGCCGAGGCGCTCGGGCTCGAGGGCGCGGTCGACAGCCGCC
GGTTCCGCGAGCTTCTCGCCGGCCGGGTGACTGCGGATGACCCGATGTCCCGCTCCGCGACGCGCCGGGACTCCCAGAGC
CGGATCGGGATCGACCTCACCTTCTCCGCTCCGAAGAGCGTCTCGCTCCAAGCGCTCGTCGGCGGGGACGAACGCATCGT
GGAGGCGCACGACCGGGCGGTCGCGCGAGCGATCGCCGCCGCCGAGGAACGCGCCCAGACGCGCAAGAAGGTGAAGGGCG
AGAGCCGGGTCGAGGACACGCGCAACCTCGTCGTCGCGAAGTTCCGTCACGAGACGAGCCGCGAGCAGGATCCCCAGCTC
CATACTCACGCCCTGGTGCTGAACCTCACCCGCCGTTCGGATGGCGCTTGGCGCGCGCTCCGCAACGACGAGATCGTGAA
GGCGACGAAGTACCTCGGCGCCATGTATCGGGCCGAGCTAGCCGCCGAACTGCAGGACGTGGGGTATGCGCTCCGGCACG
GTCGGGAGGGCACGTTCGAACTCGCCCACATGGAACGGGCGCAGCTCATCGCGTTCAGCCGGAGGACGGAACAGATCGAA
CGCCGGATGGCGCAGGAGGGGCTGACGCCCGAGAACGCGACTTCCGAGCAGAAGCAGCGGGTGAAGCTCGCGACGCGTCC
TCGGAAGGTATCGGTGGATCGCCAGGCCCTCTTCGCCGGGTGGCATGCCCGGGCACGCGAGCTCCGGATCGACCTGACCC
GACCTGCTCCCTTCGACGCGGGCCATCGTCGCCGAACGGCTTCCGGACGTCGTGACCCCGGCGCCGCAGCACACTCCGCA
GGCGAGGGCGCCCGGCGCAGTGTCCGGTTCGCGATCGCGCACCTCACCGAGCGCCAAGCCATCATCGAGGAGCGCGAGCT
GCTCGACGTCGCCCTCAAGCATGCCGTCGGCCGCGCGACGCTGCCCAACGTTCGCCGCGAGATCGCACGGCTCTCGTCTA
TCGGGTACCTCATCCCGGAGAGCCCGCTCTACCGCGCCGCCGATGCACCGATCAATGCGCCGGGCTCGCCGAAGGGGGCC
TGGGTCGCAGCGGAAGTACGTCGCGGGATGGCGCGCGACGAAGCGCGGACGCACATCGATGCGGCGATCGCGGGGGGCCG
GCTTGTACTTGTCGAGCGGCGGTTCACTACGCAGACTGCCCTGGAACGCGAGAAGCGCATCCTCCGGATCGAGCGCGACG
GGCGCGGGGCGGCGCAGCCGATCGGCGGCTCGGACCAGATCCGGAGCCGGCTCGCCTCCACTGACCTCAACGACGGGCAG
CGCGCGGCAGTGGAGCTCATCACCACGAGCCCACACCGGGTCGTCGGCGTCCAGGGATACGCCGGCACCGGGAAGAGCCA
CATGCTCGACCACGCGAAGGGACTCGCCGAGGAGCGCGGCCATCGCGTGGTCGCCCTCGCACCGTATGCGGCTCACGTCC
GCGCCCTGCGAGAGCTTGGCGTCGAGGCGAAGACGCTCGCCTCGTTCCTGGCCGCCGGCGACAAGGCGCTCGACGACAAG
ACGATGCTGGTCATCGACGAGGCGGGCACCGTCCCGACCCGCCAGATGGAACGGGCGCTCAAGCTAGCCGAGCAAGCTGG
CGCGCGCGTCGTGCTTCTCGGCGACACCGGGCAGACGAAGGCCATCGAGGCGGGGCGGCCGTTCCACCAGCTCCAGGCGG
CCGGCATGCAGACCGCCGTCATGGCCGAGATCGAACGGCAGAAGGATCCGGCGCTCCGGGAGGCCGTGTCGCTCGCTGCG
CGAGGCGAGAGCGAGTCCTCGCTCGCGCGCCTCTCCGACGTCCGCGAGGTCCGGGACGACCACGAGCGCCGCCGGGCCAT
CGCCGCCGATTACGCGCAGCTCCCCGAGGAAGAGCGCGCGAGCACCATCGTCGTCGCCGGCACGAACGAGGCGCGGCGCG
AGATCAACCGTGCGATCCGGGAGGATCTGGGCCTCGCCGGGCGCGGGCACGAGTTCGCGACGCTCACCCGGCGCGACACG
ACTCAGGCCGAGCGTGCCTTCTCCAAGAACTACTCCCCAGGCGACGTCATCCAGCCGGAGCGCGACTACCCGAAGGCGGG
GCTCTCGCGCGGCGGGCTGTACGAGGTCGTGGAGAACGGTCCCGGCAACCGGCTCACCGTACGTGCCGAGAAGGGCGAGG
TGGTCGAGTTCAGCCCCATGACCTGCCGCCGACTCTCGGTCTACGAGCCGGAGCGTTCGGAGCTCGCGGCCGGCGACCGC
GTCCGGATAACCCGGAACGACGCCGCGCGCGACCTCGCGAACGGCGACCGCTTCGCCGTGGCCGACGTGACGCGTGAGAC
CGTCACCCTCACCGACGGGAAGCGCACAGTGGAACTGCCCGCCAACAAGCCGCTCCACGTCGACCACGCCTACGCCACGA
CCGTCCACGCCAGCCAGGGGACGACCGCCGAGCGCGTTCTCATCGACGTGGCCACGAAGAGCAGAACGACGTCTCAGGAC
GTATATTACGTGGCGATCAGCAGGGCGCGGCAGGAGGCGCGGATCTACACCGACGACCTCGCGAGGCTTCCTGCGGCCGT
GGCGCGAGAGCACGCGAAGCACGCGGCGCTCGATCTCGATCGCTCAGACCGTAATAGGTAG

Upstream 100 bases:

>100_bases
CCGGCTACCTCGCGTTCGCCGGGAACCACCCCATCGCGAAGGTGAAGCTCGACGTGGTCCGTTTCCGGAACCGCGTCCCG
GCCTTCGAGGAGAGGCTGGC

Downstream 100 bases:

>100_bases
TCCTCGGGGAATTGGGCACCTCACGCCTTACCGCCGCGAATCGGCTTCCGCGCCGGAAACAAGCTCATCGTCTTACCCGG
CGGTGGGACCGGCTTCTTGC

Product: conjugative relaxase domain-containing protein

Products: NA

Alternate protein names: DNA helicase I [H]

Number of amino acids: Translated: 926; Mature: 926

Protein sequence:

>926_residues
MLSHKVLTRQDVGRAASYYEDGADDYYAGEGDSSAWQGKGAEALGLEGAVDSRRFRELLAGRVTADDPMSRSATRRDSQS
RIGIDLTFSAPKSVSLQALVGGDERIVEAHDRAVARAIAAAEERAQTRKKVKGESRVEDTRNLVVAKFRHETSREQDPQL
HTHALVLNLTRRSDGAWRALRNDEIVKATKYLGAMYRAELAAELQDVGYALRHGREGTFELAHMERAQLIAFSRRTEQIE
RRMAQEGLTPENATSEQKQRVKLATRPRKVSVDRQALFAGWHARARELRIDLTRPAPFDAGHRRRTASGRRDPGAAAHSA
GEGARRSVRFAIAHLTERQAIIEERELLDVALKHAVGRATLPNVRREIARLSSIGYLIPESPLYRAADAPINAPGSPKGA
WVAAEVRRGMARDEARTHIDAAIAGGRLVLVERRFTTQTALEREKRILRIERDGRGAAQPIGGSDQIRSRLASTDLNDGQ
RAAVELITTSPHRVVGVQGYAGTGKSHMLDHAKGLAEERGHRVVALAPYAAHVRALRELGVEAKTLASFLAAGDKALDDK
TMLVIDEAGTVPTRQMERALKLAEQAGARVVLLGDTGQTKAIEAGRPFHQLQAAGMQTAVMAEIERQKDPALREAVSLAA
RGESESSLARLSDVREVRDDHERRRAIAADYAQLPEEERASTIVVAGTNEARREINRAIREDLGLAGRGHEFATLTRRDT
TQAERAFSKNYSPGDVIQPERDYPKAGLSRGGLYEVVENGPGNRLTVRAEKGEVVEFSPMTCRRLSVYEPERSELAAGDR
VRITRNDAARDLANGDRFAVADVTRETVTLTDGKRTVELPANKPLHVDHAYATTVHASQGTTAERVLIDVATKSRTTSQD
VYYVAISRARQEARIYTDDLARLPAAVAREHAKHAALDLDRSDRNR

Sequences:

>Translated_926_residues
MLSHKVLTRQDVGRAASYYEDGADDYYAGEGDSSAWQGKGAEALGLEGAVDSRRFRELLAGRVTADDPMSRSATRRDSQS
RIGIDLTFSAPKSVSLQALVGGDERIVEAHDRAVARAIAAAEERAQTRKKVKGESRVEDTRNLVVAKFRHETSREQDPQL
HTHALVLNLTRRSDGAWRALRNDEIVKATKYLGAMYRAELAAELQDVGYALRHGREGTFELAHMERAQLIAFSRRTEQIE
RRMAQEGLTPENATSEQKQRVKLATRPRKVSVDRQALFAGWHARARELRIDLTRPAPFDAGHRRRTASGRRDPGAAAHSA
GEGARRSVRFAIAHLTERQAIIEERELLDVALKHAVGRATLPNVRREIARLSSIGYLIPESPLYRAADAPINAPGSPKGA
WVAAEVRRGMARDEARTHIDAAIAGGRLVLVERRFTTQTALEREKRILRIERDGRGAAQPIGGSDQIRSRLASTDLNDGQ
RAAVELITTSPHRVVGVQGYAGTGKSHMLDHAKGLAEERGHRVVALAPYAAHVRALRELGVEAKTLASFLAAGDKALDDK
TMLVIDEAGTVPTRQMERALKLAEQAGARVVLLGDTGQTKAIEAGRPFHQLQAAGMQTAVMAEIERQKDPALREAVSLAA
RGESESSLARLSDVREVRDDHERRRAIAADYAQLPEEERASTIVVAGTNEARREINRAIREDLGLAGRGHEFATLTRRDT
TQAERAFSKNYSPGDVIQPERDYPKAGLSRGGLYEVVENGPGNRLTVRAEKGEVVEFSPMTCRRLSVYEPERSELAAGDR
VRITRNDAARDLANGDRFAVADVTRETVTLTDGKRTVELPANKPLHVDHAYATTVHASQGTTAERVLIDVATKSRTTSQD
VYYVAISRARQEARIYTDDLARLPAAVAREHAKHAALDLDRSDRNR
>Mature_926_residues
MLSHKVLTRQDVGRAASYYEDGADDYYAGEGDSSAWQGKGAEALGLEGAVDSRRFRELLAGRVTADDPMSRSATRRDSQS
RIGIDLTFSAPKSVSLQALVGGDERIVEAHDRAVARAIAAAEERAQTRKKVKGESRVEDTRNLVVAKFRHETSREQDPQL
HTHALVLNLTRRSDGAWRALRNDEIVKATKYLGAMYRAELAAELQDVGYALRHGREGTFELAHMERAQLIAFSRRTEQIE
RRMAQEGLTPENATSEQKQRVKLATRPRKVSVDRQALFAGWHARARELRIDLTRPAPFDAGHRRRTASGRRDPGAAAHSA
GEGARRSVRFAIAHLTERQAIIEERELLDVALKHAVGRATLPNVRREIARLSSIGYLIPESPLYRAADAPINAPGSPKGA
WVAAEVRRGMARDEARTHIDAAIAGGRLVLVERRFTTQTALEREKRILRIERDGRGAAQPIGGSDQIRSRLASTDLNDGQ
RAAVELITTSPHRVVGVQGYAGTGKSHMLDHAKGLAEERGHRVVALAPYAAHVRALRELGVEAKTLASFLAAGDKALDDK
TMLVIDEAGTVPTRQMERALKLAEQAGARVVLLGDTGQTKAIEAGRPFHQLQAAGMQTAVMAEIERQKDPALREAVSLAA
RGESESSLARLSDVREVRDDHERRRAIAADYAQLPEEERASTIVVAGTNEARREINRAIREDLGLAGRGHEFATLTRRDT
TQAERAFSKNYSPGDVIQPERDYPKAGLSRGGLYEVVENGPGNRLTVRAEKGEVVEFSPMTCRRLSVYEPERSELAAGDR
VRITRNDAARDLANGDRFAVADVTRETVTLTDGKRTVELPANKPLHVDHAYATTVHASQGTTAERVLIDVATKSRTTSQD
VYYVAISRARQEARIYTDDLARLPAAVAREHAKHAALDLDRSDRNR

Specific function: TraI has been identified as DNA helicase I and it also has an additional activity of site-specific nicking at oriT. DNA helicase I is a potent DNA-dependent ATPase [H]

COG id: COG0507

COG function: function code L; ATP-dependent exoDNAse (exonuclease V), alpha subunit - helicase superfamily I member

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To traI of plasmid IncFII R100 [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014059
- InterPro:   IPR014129
- InterPro:   IPR009767
- InterPro:   IPR014862 [H]

Pfam domain/function: PF07057 TraI; PF08751 TrwC [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 101943; Mature: 101943

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSHKVLTRQDVGRAASYYEDGADDYYAGEGDSSAWQGKGAEALGLEGAVDSRRFRELLA
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
GRVTADDPMSRSATRRDSQSRIGIDLTFSAPKSVSLQALVGGDERIVEAHDRAVARAIAA
CCCCCCCCCCHHHHCCCCCCCCCEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHH
AEERAQTRKKVKGESRVEDTRNLVVAKFRHETSREQDPQLHTHALVLNLTRRSDGAWRAL
HHHHHHHHHHHCCCHHHHHHHHHEEHHHHHHCCCCCCCCHHHEEEEEEEECCCCCHHHHH
RNDEIVKATKYLGAMYRAELAAELQDVGYALRHGREGTFELAHMERAQLIAFSRRTEQIE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHH
RRMAQEGLTPENATSEQKQRVKLATRPRKVSVDRQALFAGWHARARELRIDLTRPAPFDA
HHHHHHCCCCCCCCHHHHHHHHHHCCCCEECCCHHHHHHHHHHCCEEEEEEECCCCCCCC
GHRRRTASGRRDPGAAAHSAGEGARRSVRFAIAHLTERQAIIEERELLDVALKHAVGRAT
CCCCCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LPNVRREIARLSSIGYLIPESPLYRAADAPINAPGSPKGAWVAAEVRRGMARDEARTHID
CHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHCCHHHHHHHHHHH
AAIAGGRLVLVERRFTTQTALEREKRILRIERDGRGAAQPIGGSDQIRSRLASTDLNDGQ
HHCCCCEEEEEECCHHHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCC
RAAVELITTSPHRVVGVQGYAGTGKSHMLDHAKGLAEERGHRVVALAPYAAHVRALRELG
EEEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHC
VEAKTLASFLAAGDKALDDKTMLVIDEAGTVPTRQMERALKLAEQAGARVVLLGDTGQTK
CCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCC
AIEAGRPFHQLQAAGMQTAVMAEIERQKDPALREAVSLAARGESESSLARLSDVREVRDD
EECCCCCHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
HERRRAIAADYAQLPEEERASTIVVAGTNEARREINRAIREDLGLAGRGHEFATLTRRDT
HHHHHHHHHHHHHCCCHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCH
TQAERAFSKNYSPGDVIQPERDYPKAGLSRGGLYEVVENGPGNRLTVRAEKGEVVEFSPM
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEEECCCCEEEECCC
TCRRLSVYEPERSELAAGDRVRITRNDAARDLANGDRFAVADVTRETVTLTDGKRTVELP
CEEECCCCCCCHHHHCCCCEEEEECCHHHHHHCCCCEEEEEECCCCEEEEECCCEEEECC
ANKPLHVDHAYATTVHASQGTTAERVLIDVATKSRTTSQDVYYVAISRARQEARIYTDDL
CCCCEEEECCEEEEEECCCCCCHHEEEEEEECCCCCCCCCEEEEEEHHHHHHHHHHHHHH
ARLPAAVAREHAKHAALDLDRSDRNR
HHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLSHKVLTRQDVGRAASYYEDGADDYYAGEGDSSAWQGKGAEALGLEGAVDSRRFRELLA
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
GRVTADDPMSRSATRRDSQSRIGIDLTFSAPKSVSLQALVGGDERIVEAHDRAVARAIAA
CCCCCCCCCCHHHHCCCCCCCCCEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHH
AEERAQTRKKVKGESRVEDTRNLVVAKFRHETSREQDPQLHTHALVLNLTRRSDGAWRAL
HHHHHHHHHHHCCCHHHHHHHHHEEHHHHHHCCCCCCCCHHHEEEEEEEECCCCCHHHHH
RNDEIVKATKYLGAMYRAELAAELQDVGYALRHGREGTFELAHMERAQLIAFSRRTEQIE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHH
RRMAQEGLTPENATSEQKQRVKLATRPRKVSVDRQALFAGWHARARELRIDLTRPAPFDA
HHHHHHCCCCCCCCHHHHHHHHHHCCCCEECCCHHHHHHHHHHCCEEEEEEECCCCCCCC
GHRRRTASGRRDPGAAAHSAGEGARRSVRFAIAHLTERQAIIEERELLDVALKHAVGRAT
CCCCCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LPNVRREIARLSSIGYLIPESPLYRAADAPINAPGSPKGAWVAAEVRRGMARDEARTHID
CHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHCCHHHHHHHHHHH
AAIAGGRLVLVERRFTTQTALEREKRILRIERDGRGAAQPIGGSDQIRSRLASTDLNDGQ
HHCCCCEEEEEECCHHHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCC
RAAVELITTSPHRVVGVQGYAGTGKSHMLDHAKGLAEERGHRVVALAPYAAHVRALRELG
EEEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHC
VEAKTLASFLAAGDKALDDKTMLVIDEAGTVPTRQMERALKLAEQAGARVVLLGDTGQTK
CCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCC
AIEAGRPFHQLQAAGMQTAVMAEIERQKDPALREAVSLAARGESESSLARLSDVREVRDD
EECCCCCHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
HERRRAIAADYAQLPEEERASTIVVAGTNEARREINRAIREDLGLAGRGHEFATLTRRDT
HHHHHHHHHHHHHCCCHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCH
TQAERAFSKNYSPGDVIQPERDYPKAGLSRGGLYEVVENGPGNRLTVRAEKGEVVEFSPM
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEEECCCCEEEECCC
TCRRLSVYEPERSELAAGDRVRITRNDAARDLANGDRFAVADVTRETVTLTDGKRTVELP
CEEECCCCCCCHHHHCCCCEEEEECCHHHHHHCCCCEEEEEECCCCEEEEECCCEEEECC
ANKPLHVDHAYATTVHASQGTTAERVLIDVATKSRTTSQDVYYVAISRARQEARIYTDDL
CCCCEEEECCEEEEEECCCCCCHHEEEEEEECCCCCCCCCEEEEEEHHHHHHHHHHHHHH
ARLPAAVAREHAKHAALDLDRSDRNR
HHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2163400; 7915817; 2680768; 2164585; 8736534 [H]