Click here to switch to the map view.

The map label for this gene is phoA [H]

Identifier: 220916154

GI number: 220916154

Start: 1166033

End: 1167469

Strand: Reverse

Name: phoA [H]

Synonym: A2cp1_1041

Alternate gene names: 220916154

Gene position: 1167469-1166033 (Counterclockwise)

Preceding gene: 220916155

Following gene: 220916153

Centisome position: 23.21

GC content: 74.53

Gene sequence:

>1437_bases
ATGCGATCTTCACCCGGCTCCTTCTCCCACCGCGCGCTCGCGGCGCTCGCCGCGGGCGCCCTCGCGCTCGGCGGCTGCTC
GTCCGACTCCTCGTCGAGCCCGCCCCCGGCCCCGCCGCGCAACGTCCTCTTCTTCCTGGGCGACGGCATGGGCATGACCA
CGCTCACCGCCGCGCGCATCTACTCGGTCGGCGAGGACGGCGCGCTCACCATCGACGCGCTGCCCGAGACCGGCTTCGTC
AAGACCTACTCGAACGACGCCCAGGTCACCGACAGCGCGCCGTCCATGGCCGCGTACATGACCGGCGTGAAGGCGAACAA
CGAGGTCATCTCGATGTCCCCCGACACGCGCGCGTTCGACGCCGCCGGCGTCGACTACCTCTCCGGCGCGGACAGCGCCT
GCCCGCCCAGCGGCAACGGCGCGCCCGTGACCACGCTGCTCGAGCTCGCGAAGGCGGCCGGCCGCGCCACCGGCGTCGTG
ACCACCACCCGCGTCACCCACGCCACGCCGGCCGCGACCTACGCCCACGTCTGCCACCGCGACGCCGAGAACACGATCGC
CGCGCAGCTCGTCCCGGGCGCCGGCTTCAACGCGGCGCTCGGCTCGGGGGTCGACGTGGTGCTGGGCGGCGGCCGCCGGC
ACTTCCTCCCCGCCGCCAAGGGCGGCGCCCGCACCGACGGGCGCGACCTCGTCGCCGAGCTGAAGGCGGCGGGCTGGGCC
TACGCCTCCACCGAGGCGGAGCTGGACGCCGCGCCGGCCGGCGGCCGGCTCCTCGGCCTGTTCACGGGAAGCCACATGGC
CTACGACCTCGACCGCGACCCGGCCGCGCAGCCCAGCCTGGCCGAGATGACCGGGAAGGCGCTCGACGTGCTCACGCGCA
ACCCGAAGGGCTTCTTCTTGATGGTCGAGGGCGGGCGCATCGACCACGCCCTCCACGAGACCACCGCGAAGAAGGCGCTC
CAGGACACCGTGGCGTTCGACCACGCCATCGCGACCGCGCTCGCGCGCATGCAGCAGGTCGACCCCGGCCTGAAGAACAC
GCTCGTGGTGGTCACCGCCGACCACGACCACACGCTCGTCCTGAACGGCTACGCGCGGCGGACGGGCCCGACCACCGCGG
CCGCCCCAGGTGTCCTCGGCCTCGTCCGCAACGTGCTCACCGGCGCGCCCGACCCGGACGTGGGCGGGCTCCCGTACACG
GTGATCGGCTTCGGGAACGGCGAGAGCCGCCCGGCCACGCGCACCGCGCTCGACGACGCGACCGTCTCCGCGAGCACCTA
CCACCAGGAGGCCGCCGTCCCCATGCCGGCCGGCGGCGAGACCCACGGCGGCACCGACGTGTTCATCGGCGCAGCCGGGC
TCGGCGCGGAGCGCTTCACCGGGGTCATCGACAACACCGCCGTCTTCACGCTGGTCCGCCGGGCCGCGGGCCTCTAG

Upstream 100 bases:

>100_bases
CTGGGCGCTGCGCGAGAAGCTCACGCGCTCGGCACCGGCTCGCCACGCGGCGGCGCCTATACCCGGCTCCTCCCACTCCA
CGCAAAGGACGGTCCTTCGC

Downstream 100 bases:

>100_bases
CCCTCGCTCCCAGGAGACACTCCCATGCACGCACGCACCCTCGCGGCGCTCGCGCTCGCGCTCTCCGCCGGCGACGCCGT
CGCCGCCGGCCCGGCCAAGA

Product: Alkaline phosphatase

Products: NA

Alternate protein names: APase [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MRSSPGSFSHRALAALAAGALALGGCSSDSSSSPPPAPPRNVLFFLGDGMGMTTLTAARIYSVGEDGALTIDALPETGFV
KTYSNDAQVTDSAPSMAAYMTGVKANNEVISMSPDTRAFDAAGVDYLSGADSACPPSGNGAPVTTLLELAKAAGRATGVV
TTTRVTHATPAATYAHVCHRDAENTIAAQLVPGAGFNAALGSGVDVVLGGGRRHFLPAAKGGARTDGRDLVAELKAAGWA
YASTEAELDAAPAGGRLLGLFTGSHMAYDLDRDPAAQPSLAEMTGKALDVLTRNPKGFFLMVEGGRIDHALHETTAKKAL
QDTVAFDHAIATALARMQQVDPGLKNTLVVVTADHDHTLVLNGYARRTGPTTAAAPGVLGLVRNVLTGAPDPDVGGLPYT
VIGFGNGESRPATRTALDDATVSASTYHQEAAVPMPAGGETHGGTDVFIGAAGLGAERFTGVIDNTAVFTLVRRAAGL

Sequences:

>Translated_478_residues
MRSSPGSFSHRALAALAAGALALGGCSSDSSSSPPPAPPRNVLFFLGDGMGMTTLTAARIYSVGEDGALTIDALPETGFV
KTYSNDAQVTDSAPSMAAYMTGVKANNEVISMSPDTRAFDAAGVDYLSGADSACPPSGNGAPVTTLLELAKAAGRATGVV
TTTRVTHATPAATYAHVCHRDAENTIAAQLVPGAGFNAALGSGVDVVLGGGRRHFLPAAKGGARTDGRDLVAELKAAGWA
YASTEAELDAAPAGGRLLGLFTGSHMAYDLDRDPAAQPSLAEMTGKALDVLTRNPKGFFLMVEGGRIDHALHETTAKKAL
QDTVAFDHAIATALARMQQVDPGLKNTLVVVTADHDHTLVLNGYARRTGPTTAAAPGVLGLVRNVLTGAPDPDVGGLPYT
VIGFGNGESRPATRTALDDATVSASTYHQEAAVPMPAGGETHGGTDVFIGAAGLGAERFTGVIDNTAVFTLVRRAAGL
>Mature_478_residues
MRSSPGSFSHRALAALAAGALALGGCSSDSSSSPPPAPPRNVLFFLGDGMGMTTLTAARIYSVGEDGALTIDALPETGFV
KTYSNDAQVTDSAPSMAAYMTGVKANNEVISMSPDTRAFDAAGVDYLSGADSACPPSGNGAPVTTLLELAKAAGRATGVV
TTTRVTHATPAATYAHVCHRDAENTIAAQLVPGAGFNAALGSGVDVVLGGGRRHFLPAAKGGARTDGRDLVAELKAAGWA
YASTEAELDAAPAGGRLLGLFTGSHMAYDLDRDPAAQPSLAEMTGKALDVLTRNPKGFFLMVEGGRIDHALHETTAKKAL
QDTVAFDHAIATALARMQQVDPGLKNTLVVVTADHDHTLVLNGYARRTGPTTAAAPGVLGLVRNVLTGAPDPDVGGLPYT
VIGFGNGESRPATRTALDDATVSASTYHQEAAVPMPAGGETHGGTDVFIGAAGLGAERFTGVIDNTAVFTLVRRAAGL

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI157266296, Length=478, Percent_Identity=37.4476987447699, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI157266292, Length=470, Percent_Identity=36.8085106382979, Blast_Score=240, Evalue=2e-63,
Organism=Homo sapiens, GI94721246, Length=478, Percent_Identity=37.0292887029289, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI116734717, Length=474, Percent_Identity=35.8649789029536, Blast_Score=236, Evalue=3e-62,
Organism=Homo sapiens, GI294660770, Length=470, Percent_Identity=35.7446808510638, Blast_Score=230, Evalue=2e-60,
Organism=Homo sapiens, GI294660772, Length=434, Percent_Identity=34.331797235023, Blast_Score=198, Evalue=9e-51,
Organism=Escherichia coli, GI48994877, Length=482, Percent_Identity=31.5352697095436, Blast_Score=149, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6320689, Length=341, Percent_Identity=35.1906158357771, Blast_Score=166, Evalue=6e-42,
Organism=Drosophila melanogaster, GI21355151, Length=460, Percent_Identity=37.6086956521739, Blast_Score=245, Evalue=5e-65,
Organism=Drosophila melanogaster, GI85725200, Length=465, Percent_Identity=35.4838709677419, Blast_Score=244, Evalue=9e-65,
Organism=Drosophila melanogaster, GI85815833, Length=465, Percent_Identity=35.4838709677419, Blast_Score=244, Evalue=9e-65,
Organism=Drosophila melanogaster, GI21355981, Length=471, Percent_Identity=35.6687898089172, Blast_Score=243, Evalue=2e-64,
Organism=Drosophila melanogaster, GI19921912, Length=484, Percent_Identity=35.5371900826446, Blast_Score=240, Evalue=2e-63,
Organism=Drosophila melanogaster, GI21358067, Length=541, Percent_Identity=33.826247689464, Blast_Score=239, Evalue=3e-63,
Organism=Drosophila melanogaster, GI21355149, Length=475, Percent_Identity=33.2631578947368, Blast_Score=225, Evalue=5e-59,
Organism=Drosophila melanogaster, GI24585213, Length=476, Percent_Identity=35.7142857142857, Blast_Score=223, Evalue=3e-58,
Organism=Drosophila melanogaster, GI18859923, Length=457, Percent_Identity=35.4485776805252, Blast_Score=222, Evalue=3e-58,
Organism=Drosophila melanogaster, GI24651554, Length=499, Percent_Identity=33.6673346693387, Blast_Score=199, Evalue=3e-51,
Organism=Drosophila melanogaster, GI24657835, Length=488, Percent_Identity=32.9918032786885, Blast_Score=190, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24657842, Length=487, Percent_Identity=32.2381930184805, Blast_Score=186, Evalue=4e-47,
Organism=Drosophila melanogaster, GI24651556, Length=489, Percent_Identity=33.1288343558282, Blast_Score=182, Evalue=5e-46,
Organism=Drosophila melanogaster, GI24657827, Length=477, Percent_Identity=32.2851153039832, Blast_Score=180, Evalue=2e-45,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 48589; Mature: 48589

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSSPGSFSHRALAALAAGALALGGCSSDSSSSPPPAPPRNVLFFLGDGMGMTTLTAARI
CCCCCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHH
YSVGEDGALTIDALPETGFVKTYSNDAQVTDSAPSMAAYMTGVKANNEVISMSPDTRAFD
EECCCCCCEEEECCCCCCEEEEECCCCEECCCCCCHHHHHHCCCCCCCEEEECCCCCCCC
AAGVDYLSGADSACPPSGNGAPVTTLLELAKAAGRATGVVTTTRVTHATPAATYAHVCHR
CCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHC
DAENTIAAQLVPGAGFNAALGSGVDVVLGGGRRHFLPAAKGGARTDGRDLVAELKAAGWA
CCCCCEEEEEECCCCCCHHHCCCCEEEEECCCEEECCCCCCCCCCCHHHHHHHHHHCCCE
YASTEAELDAAPAGGRLLGLFTGSHMAYDLDRDPAAQPSLAEMTGKALDVLTRNPKGFFL
EECCCCCCCCCCCCCEEEEEEECCEEEEECCCCCCCCCCHHHHCCHHHHHHCCCCCCEEE
MVEGGRIDHALHETTAKKALQDTVAFDHAIATALARMQQVDPGLKNTLVVVTADHDHTLV
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEE
LNGYARRTGPTTAAAPGVLGLVRNVLTGAPDPDVGGLPYTVIGFGNGESRPATRTALDDA
EECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHCCC
TVSASTYHQEAAVPMPAGGETHGGTDVFIGAAGLGAERFTGVIDNTAVFTLVRRAAGL
CCCHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure
MRSSPGSFSHRALAALAAGALALGGCSSDSSSSPPPAPPRNVLFFLGDGMGMTTLTAARI
CCCCCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHH
YSVGEDGALTIDALPETGFVKTYSNDAQVTDSAPSMAAYMTGVKANNEVISMSPDTRAFD
EECCCCCCEEEECCCCCCEEEEECCCCEECCCCCCHHHHHHCCCCCCCEEEECCCCCCCC
AAGVDYLSGADSACPPSGNGAPVTTLLELAKAAGRATGVVTTTRVTHATPAATYAHVCHR
CCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHC
DAENTIAAQLVPGAGFNAALGSGVDVVLGGGRRHFLPAAKGGARTDGRDLVAELKAAGWA
CCCCCEEEEEECCCCCCHHHCCCCEEEEECCCEEECCCCCCCCCCCHHHHHHHHHHCCCE
YASTEAELDAAPAGGRLLGLFTGSHMAYDLDRDPAAQPSLAEMTGKALDVLTRNPKGFFL
EECCCCCCCCCCCCCEEEEEEECCEEEEECCCCCCCCCCHHHHCCHHHHHHCCCCCCEEE
MVEGGRIDHALHETTAKKALQDTVAFDHAIATALARMQQVDPGLKNTLVVVTADHDHTLV
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEE
LNGYARRTGPTTAAAPGVLGLVRNVLTGAPDPDVGGLPYTVIGFGNGESRPATRTALDDA
EECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHCCC
TVSASTYHQEAAVPMPAGGETHGGTDVFIGAAGLGAERFTGVIDNTAVFTLVRRAAGL
CCCHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2129542 [H]