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The map label for this gene is pilR [H]

Identifier: 220915779

GI number: 220915779

Start: 735580

End: 736965

Strand: Direct

Name: pilR [H]

Synonym: A2cp1_0660

Alternate gene names: 220915779

Gene position: 735580-736965 (Clockwise)

Preceding gene: 220915778

Following gene: 220915789

Centisome position: 14.63

GC content: 73.52

Gene sequence:

>1386_bases
ATGGCGAAGATCCTGGTGGTCGACGACGAGCAGTCGATGCGCGAGTTCCTCGAGATCCTGCTGCGCAAGCAGGGCCACGA
GGTGGTCGCGGCCGCGACCCTCGGAGCCGCGCTCGCGTGCGCCGCGGAGGGCGATCTCGACCTCGTCCTCTCCGACCTGC
GCCTGGGGGCGGAGAGCGGGATCCAGCTGCTCGAGGCGGTCAAGCGCGAGGCGCCCGGGACGGAGGTGATCATCCTCACC
GCGTTCGCCACCACCGAGAACGCCATCCAGGCGATGAAGCTCGGCGCGTACGACTACGTGCTGAAGCCGTTCAAGGTCGA
GGAGCTGAAGCTGGTGATCGAGAAGGCGCTCGAGCACCGCGGGCTCGTCGCCGAGAACCGGGTCCTCCGGCACAGGGTGG
GCCGGCGCGGCGACGACGGGCAGGACGCCGAGCTCATCGGCCGGTCGCCTGCCATCGAGGAGGTCCGCGCCCTGGTGGAG
AAGCTGGCGCGAACCCGCACCACCGTGCTCATCAGCGGCGAGAGCGGGACCGGCAAGGAGGTGGTGGCGCGCACCATCCA
CGCCCGCGCCGGGCATCGGCAGCCGTTCGTCGCCATCAACTGCGGGGCCATCCCCGAGGGCCTGATCGAGAGCGAGCTGT
TCGGCCACGAGAAGGGCAGCTTCACCGGCGCGACCGAGGCGAAGGCCGGGCTGTTCGAGGTGGCCGGGGCGGGGACGCTG
TTCCTGGACGAGGTGGGCGAGCTGCCGCCGCCGGTGCAGGTCAAGCTGCTGCGGGCCCTCCAGGAGCGGAAGCTCCGCCG
AGTGGGCGGGAGCGCCGACCTGGGCTTCTCCGCGCGCATCGTGGCGGCCACCAACCGCGACCTCGCCGAGGAGGTCCGGA
CGGGCCGGTTCCGCGAGGACCTCTACTACCGGCTGAACGTCATCCAGCTCCGCATGCCCGCCCTGCGCGAGCGGCGCGAG
GATCTGCCGCTGTTCGTCGAGCACTTCCTGGCCCGCTTCGCCGAGGAGCAGGGCCGGGCGCGACCCGCGCTGAGCCCGGA
GGCGGAGCGCCTGCTGCTCGCCCACGGCTACCCGGGCAACGTGCGCGAGCTCGCGAACGTGGTGGAGCGCGCGCTCACGC
TCTGCGACGGGGGCCGGATCCTCCCGGGCGACCTCCCGCACGCGCTCCGCGGCGCCGCCGCCCCGGCCCCCGCGGCCGGC
GCGGCGCCCGCGCTCCCGGACGCGGGGATCGACCTCCAGGCGCACCTGGACGCGATCGAGCGCGCCCTGCTGGAGCAGGC
CCTGGAGCGCACCGGCGGCGTGAAGACGGAGGCCGCGCGCGTGCTCTCGCTCAGCTTTCGCTCGCTGCGGTACCGCCTGG
CCAAGTTCGGGATCGCCGGCCGCTGA

Upstream 100 bases:

>100_bases
GCGGGTCGAGGTGCGCTCCGCCCCGGGCCAGGGCGCCCGGTTCACGGTCCACCTGCCCGGGCCCGCGCGCGCGGCGGAGA
CAGGGTAGGATCGGGGGGCC

Downstream 100 bases:

>100_bases
GCCGGCGATCCCGTTCCGTCACTCGCAGCTGGCCGCGTCGGAGGTGGCCGCGTGCCGGCACGCGTGCAGCGGCTCGCTCA
CCTCCATGAACCCGAAGTCC

Product: two component sigma-54 specific Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MAKILVVDDEQSMREFLEILLRKQGHEVVAAATLGAALACAAEGDLDLVLSDLRLGAESGIQLLEAVKREAPGTEVIILT
AFATTENAIQAMKLGAYDYVLKPFKVEELKLVIEKALEHRGLVAENRVLRHRVGRRGDDGQDAELIGRSPAIEEVRALVE
KLARTRTTVLISGESGTGKEVVARTIHARAGHRQPFVAINCGAIPEGLIESELFGHEKGSFTGATEAKAGLFEVAGAGTL
FLDEVGELPPPVQVKLLRALQERKLRRVGGSADLGFSARIVAATNRDLAEEVRTGRFREDLYYRLNVIQLRMPALRERRE
DLPLFVEHFLARFAEEQGRARPALSPEAERLLLAHGYPGNVRELANVVERALTLCDGGRILPGDLPHALRGAAAPAPAAG
AAPALPDAGIDLQAHLDAIERALLEQALERTGGVKTEAARVLSLSFRSLRYRLAKFGIAGR

Sequences:

>Translated_461_residues
MAKILVVDDEQSMREFLEILLRKQGHEVVAAATLGAALACAAEGDLDLVLSDLRLGAESGIQLLEAVKREAPGTEVIILT
AFATTENAIQAMKLGAYDYVLKPFKVEELKLVIEKALEHRGLVAENRVLRHRVGRRGDDGQDAELIGRSPAIEEVRALVE
KLARTRTTVLISGESGTGKEVVARTIHARAGHRQPFVAINCGAIPEGLIESELFGHEKGSFTGATEAKAGLFEVAGAGTL
FLDEVGELPPPVQVKLLRALQERKLRRVGGSADLGFSARIVAATNRDLAEEVRTGRFREDLYYRLNVIQLRMPALRERRE
DLPLFVEHFLARFAEEQGRARPALSPEAERLLLAHGYPGNVRELANVVERALTLCDGGRILPGDLPHALRGAAAPAPAAG
AAPALPDAGIDLQAHLDAIERALLEQALERTGGVKTEAARVLSLSFRSLRYRLAKFGIAGR
>Mature_460_residues
AKILVVDDEQSMREFLEILLRKQGHEVVAAATLGAALACAAEGDLDLVLSDLRLGAESGIQLLEAVKREAPGTEVIILTA
FATTENAIQAMKLGAYDYVLKPFKVEELKLVIEKALEHRGLVAENRVLRHRVGRRGDDGQDAELIGRSPAIEEVRALVEK
LARTRTTVLISGESGTGKEVVARTIHARAGHRQPFVAINCGAIPEGLIESELFGHEKGSFTGATEAKAGLFEVAGAGTLF
LDEVGELPPPVQVKLLRALQERKLRRVGGSADLGFSARIVAATNRDLAEEVRTGRFREDLYYRLNVIQLRMPALRERRED
LPLFVEHFLARFAEEQGRARPALSPEAERLLLAHGYPGNVRELANVVERALTLCDGGRILPGDLPHALRGAAAPAPAAGA
APALPDAGIDLQAHLDAIERALLEQALERTGGVKTEAARVLSLSFRSLRYRLAKFGIAGR

Specific function: Member of the two-component regulatory system pilS/pilR that regulates the expression of type 4 fimbriae. When activated by pilS it acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=459, Percent_Identity=42.7015250544662, Blast_Score=371, Evalue=1e-104,
Organism=Escherichia coli, GI1790437, Length=455, Percent_Identity=43.956043956044, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI1790299, Length=473, Percent_Identity=38.6892177589852, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI1788905, Length=441, Percent_Identity=39.2290249433107, Blast_Score=296, Evalue=3e-81,
Organism=Escherichia coli, GI87082117, Length=334, Percent_Identity=44.0119760479042, Blast_Score=252, Evalue=3e-68,
Organism=Escherichia coli, GI1789233, Length=327, Percent_Identity=39.4495412844037, Blast_Score=242, Evalue=3e-65,
Organism=Escherichia coli, GI1789087, Length=341, Percent_Identity=42.2287390029325, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI87082152, Length=320, Percent_Identity=41.875, Blast_Score=222, Evalue=4e-59,
Organism=Escherichia coli, GI1786524, Length=335, Percent_Identity=40.2985074626866, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1787583, Length=326, Percent_Identity=38.3435582822086, Blast_Score=212, Evalue=4e-56,
Organism=Escherichia coli, GI87081872, Length=319, Percent_Identity=39.4984326018809, Blast_Score=191, Evalue=7e-50,
Organism=Escherichia coli, GI1789828, Length=244, Percent_Identity=36.8852459016393, Blast_Score=144, Evalue=8e-36,
Organism=Escherichia coli, GI87081858, Length=299, Percent_Identity=28.4280936454849, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1786599, Length=134, Percent_Identity=32.8358208955224, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1786911, Length=117, Percent_Identity=36.7521367521368, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49928; Mature: 49797

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKILVVDDEQSMREFLEILLRKQGHEVVAAATLGAALACAAEGDLDLVLSDLRLGAESG
CCEEEEECCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHH
IQLLEAVKREAPGTEVIILTAFATTENAIQAMKLGAYDYVLKPFKVEELKLVIEKALEHR
HHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHC
GLVAENRVLRHRVGRRGDDGQDAELIGRSPAIEEVRALVEKLARTRTTVLISGESGTGKE
CCHHHHHHHHHHCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCHH
VVARTIHARAGHRQPFVAINCGAIPEGLIESELFGHEKGSFTGATEAKAGLFEVAGAGTL
HHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCHHHCCHHHHCCCCEE
FLDEVGELPPPVQVKLLRALQERKLRRVGGSADLGFSARIVAATNRDLAEEVRTGRFRED
HHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHCCHHHH
LYYRLNVIQLRMPALRERREDLPLFVEHFLARFAEEQGRARPALSPEAERLLLAHGYPGN
HHHHHHHHEECCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCC
VRELANVVERALTLCDGGRILPGDLPHALRGAAAPAPAAGAAPALPDAGIDLQAHLDAIE
HHHHHHHHHHHHHHHCCCEECCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
RALLEQALERTGGVKTEAARVLSLSFRSLRYRLAKFGIAGR
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AKILVVDDEQSMREFLEILLRKQGHEVVAAATLGAALACAAEGDLDLVLSDLRLGAESG
CEEEEECCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHH
IQLLEAVKREAPGTEVIILTAFATTENAIQAMKLGAYDYVLKPFKVEELKLVIEKALEHR
HHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHC
GLVAENRVLRHRVGRRGDDGQDAELIGRSPAIEEVRALVEKLARTRTTVLISGESGTGKE
CCHHHHHHHHHHCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCHH
VVARTIHARAGHRQPFVAINCGAIPEGLIESELFGHEKGSFTGATEAKAGLFEVAGAGTL
HHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCHHHCCHHHHCCCCEE
FLDEVGELPPPVQVKLLRALQERKLRRVGGSADLGFSARIVAATNRDLAEEVRTGRFRED
HHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHCCHHHH
LYYRLNVIQLRMPALRERREDLPLFVEHFLARFAEEQGRARPALSPEAERLLLAHGYPGN
HHHHHHHHEECCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCC
VRELANVVERALTLCDGGRILPGDLPHALRGAAAPAPAAGAAPALPDAGIDLQAHLDAIE
HHHHHHHHHHHHHHHCCCEECCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
RALLEQALERTGGVKTEAARVLSLSFRSLRYRLAKFGIAGR
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1317379; 8097014; 7911557; 10984043 [H]