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The map label for this gene is nuoN

Identifier: 220915563

GI number: 220915563

Start: 504052

End: 505527

Strand: Direct

Name: nuoN

Synonym: A2cp1_0444

Alternate gene names: 220915563

Gene position: 504052-505527 (Clockwise)

Preceding gene: 220915562

Following gene: 220915573

Centisome position: 10.02

GC content: 72.09

Gene sequence:

>1476_bases
ATGAGCGGCTTCCCGACCCAGGACTTCGTCGCGCTGGTCCCGGTGGCGATCCTCACGATGGGGGCGCTGGTCCTGCTGAT
GACCGAGGTGTTCCTCACCTCGGGCCGGCGGGCCTACCAGGCCTACCTGACCATGGTGTTCGCGGCCGCCGCCGCGGGAT
ACGCCGCCTGGATGCCGGTGCCCGGCAACGTGTTCGGGCGCCAGGCGGTGGTGGACAGCTTCTCGGCCTTCGTCACGGTG
GTGCTCTGCGCCGGCCTGGCGCTTTCCGCCCTGGTGGGCCAGAGCTGGCTGAACGCCCGCAACTCCGAGCGCGGCGAGTT
CTACGCGCTGGCGCTGTTCGGCACCGCCGGCATGGTGCTGCTGGGCATGGCCACCGACCTCCTCATCGCGTTCATCGCGG
TCGAGGTGATGAGCCTCTCCACCTACTGCCTCGCCGCCTTCCTGCGGCGCGGCAAGAAGCCCGCCGAGGCGGCGTTCAAG
TACGTGGTGCTCGGCTCGATCTCGTCGGCGCTGCTGCTCTACGGGTCGGCGCTGCTCTACGGCGCGTCGGGCTCGACGCT
GTTCTCGGTGCTGCCGCGCGGCCAGGGCTCGGCGCTGTACCTCGCCGGCATCGCGCTGGTGGCCGGCGGCGTCGCCTTCA
AGATCGCCGCGGTCCCGTTCCACGCCTGGACCCCGGACGTCTACGAGGGCGCGCCCACCCCGGTGACCGCGTTCATGGCC
GCGGGCGTGAAGACCGCCGCGTTCGCGGTGCTGGTCCGCCTGTTCCTGGCCACCGAGAGCGGCTCGGCCGCCATGTCCAC
CTCGCTCGGCACGGTGCTCTCCGCCCTGGCCGTGCTGACGATGATCTTCGGCAACCTGCTCGCGCTGCCGCAGCGCAGCG
TGAAGCGGATGCTGGCGTACTCGTCGATCGGCCACGCCGGCTACCTGCTGGTGGGCGTGGTCTCCGCGGTGGTGGCGGGC
GCGCGCGACAAGGCGCTCGCGGGCCTGCTGTTCTACCTCGCGGCCTACACCGCCACCGTCATCGGCGCGTTCGCGGTGGT
CGGGGCGCTGGAGCGGCGGACCCGGGGCGACTTCGAGCCGGCCGACGCGTGGGACCTGGACCGCTTCGCCGGCCTGGCGC
GGCGCCGCCCGGCGCTCGCGTTCGCCATGGCGGTGTTCCTGTTCTCGCTCGCGGGCGTCCCGCCCACCGCCGGCTTCATC
GGCAAGTTCTACATCTTCAAGGCGGCCATGGGCGCCGGGCTCTACGGCCTGGCCGTGCTGGGCGTGCTCACCAGCGTGCT
CGGCGCGTACTACTACCTGCGCGTCGTCCTCTACATGTACTTCCTGCCGTCCAAGGACGAGGCCGGCGAGGTGGTGCTCT
CGGCCCCGTCGCTCACCATCGCGCTCGCCGCGGCGGTGGCGGTGGTGGTCCTGCTCGGCGTCGTGGCCGACCCGATGGTC
CGGCTGGCCCAGGCGGCGAGCGCCATCATCCTGTAG

Upstream 100 bases:

>100_bases
GTGATGGTGCGCGCGCCCGCGCCGGCCCCGGCGGCGGAGCCCGCCCCGCTGCCCGCGCCGGCGCCGGTCCCGCTCCAGCA
GCCCGGAGGGAATTACTGAC

Downstream 100 bases:

>100_bases
CGGCGGCGCACGCCTCACCTCACGCGCGGCGGCCGGGGCGACCGGGCCGCCGCGCCGTCGTCTCCGGGCGGCGCGCCGCG
GGGGCGGAGGGGTGGGTGGG

Product: proton-translocating NADH-quinone oxidoreductase subunit N

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MSGFPTQDFVALVPVAILTMGALVLLMTEVFLTSGRRAYQAYLTMVFAAAAAGYAAWMPVPGNVFGRQAVVDSFSAFVTV
VLCAGLALSALVGQSWLNARNSERGEFYALALFGTAGMVLLGMATDLLIAFIAVEVMSLSTYCLAAFLRRGKKPAEAAFK
YVVLGSISSALLLYGSALLYGASGSTLFSVLPRGQGSALYLAGIALVAGGVAFKIAAVPFHAWTPDVYEGAPTPVTAFMA
AGVKTAAFAVLVRLFLATESGSAAMSTSLGTVLSALAVLTMIFGNLLALPQRSVKRMLAYSSIGHAGYLLVGVVSAVVAG
ARDKALAGLLFYLAAYTATVIGAFAVVGALERRTRGDFEPADAWDLDRFAGLARRRPALAFAMAVFLFSLAGVPPTAGFI
GKFYIFKAAMGAGLYGLAVLGVLTSVLGAYYYLRVVLYMYFLPSKDEAGEVVLSAPSLTIALAAAVAVVVLLGVVADPMV
RLAQAASAIIL

Sequences:

>Translated_491_residues
MSGFPTQDFVALVPVAILTMGALVLLMTEVFLTSGRRAYQAYLTMVFAAAAAGYAAWMPVPGNVFGRQAVVDSFSAFVTV
VLCAGLALSALVGQSWLNARNSERGEFYALALFGTAGMVLLGMATDLLIAFIAVEVMSLSTYCLAAFLRRGKKPAEAAFK
YVVLGSISSALLLYGSALLYGASGSTLFSVLPRGQGSALYLAGIALVAGGVAFKIAAVPFHAWTPDVYEGAPTPVTAFMA
AGVKTAAFAVLVRLFLATESGSAAMSTSLGTVLSALAVLTMIFGNLLALPQRSVKRMLAYSSIGHAGYLLVGVVSAVVAG
ARDKALAGLLFYLAAYTATVIGAFAVVGALERRTRGDFEPADAWDLDRFAGLARRRPALAFAMAVFLFSLAGVPPTAGFI
GKFYIFKAAMGAGLYGLAVLGVLTSVLGAYYYLRVVLYMYFLPSKDEAGEVVLSAPSLTIALAAAVAVVVLLGVVADPMV
RLAQAASAIIL
>Mature_490_residues
SGFPTQDFVALVPVAILTMGALVLLMTEVFLTSGRRAYQAYLTMVFAAAAAGYAAWMPVPGNVFGRQAVVDSFSAFVTVV
LCAGLALSALVGQSWLNARNSERGEFYALALFGTAGMVLLGMATDLLIAFIAVEVMSLSTYCLAAFLRRGKKPAEAAFKY
VVLGSISSALLLYGSALLYGASGSTLFSVLPRGQGSALYLAGIALVAGGVAFKIAAVPFHAWTPDVYEGAPTPVTAFMAA
GVKTAAFAVLVRLFLATESGSAAMSTSLGTVLSALAVLTMIFGNLLALPQRSVKRMLAYSSIGHAGYLLVGVVSAVVAGA
RDKALAGLLFYLAAYTATVIGAFAVVGALERRTRGDFEPADAWDLDRFAGLARRRPALAFAMAVFLFSLAGVPPTAGFIG
KFYIFKAAMGAGLYGLAVLGVLTSVLGAYYYLRVVLYMYFLPSKDEAGEVVLSAPSLTIALAAAVAVVVLLGVVADPMVR
LAQAASAIIL

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family

Homologues:

Organism=Escherichia coli, GI145693160, Length=493, Percent_Identity=36.5111561866126, Blast_Score=251, Evalue=1e-67,
Organism=Escherichia coli, GI1788829, Length=349, Percent_Identity=28.3667621776504, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788614, Length=455, Percent_Identity=23.7362637362637, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1788827, Length=358, Percent_Identity=25.9776536312849, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1788831, Length=321, Percent_Identity=26.4797507788162, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1788613, Length=427, Percent_Identity=26.2295081967213, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI2367154, Length=377, Percent_Identity=24.6684350132626, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NUON_ANAD2 (B8JAZ0)

Other databases:

- EMBL:   CP001359
- RefSeq:   YP_002490867.1
- GeneID:   7299327
- GenomeReviews:   CP001359_GR
- KEGG:   acp:A2cp1_0444
- HOGENOM:   HBG747830
- ProtClustDB:   CLSK945022
- GO:   GO:0006810
- HAMAP:   MF_00445
- InterPro:   IPR010096
- InterPro:   IPR001750
- TIGRFAMs:   TIGR01770

Pfam domain/function: PF00361 Oxidored_q1

EC number: =1.6.99.5

Molecular weight: Translated: 50940; Mature: 50809

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x19d12ad0)-; HASH(0x40bfab44)-; HASH(0x40bf977c)-; HASH(0x40bf9f50)-; HASH(0x1a071b44)-; HASH(0x40bfa658)-; HASH(0x40bfb2dc)-; HASH(0x40bf9f98)-; HASH(0x40bf83f0)-; HASH(0x40bfb4f8)-; HASH(0x40bf9a64)-; HASH(0x40bf9f38)-; HASH(0x40bfb540)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGFPTQDFVALVPVAILTMGALVLLMTEVFLTSGRRAYQAYLTMVFAAAAAGYAAWMPV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEECCC
PGNVFGRQAVVDSFSAFVTVVLCAGLALSALVGQSWLNARNSERGEFYALALFGTAGMVL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHH
LGMATDLLIAFIAVEVMSLSTYCLAAFLRRGKKPAEAAFKYVVLGSISSALLLYGSALLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GASGSTLFSVLPRGQGSALYLAGIALVAGGVAFKIAAVPFHAWTPDVYEGAPTPVTAFMA
CCCCCHHHHHCCCCCCCCHHHHHHHHHHCCHHHEEEEECCCCCCCCCCCCCCCHHHHHHH
AGVKTAAFAVLVRLFLATESGSAAMSTSLGTVLSALAVLTMIFGNLLALPQRSVKRMLAY
HCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
SSIGHAGYLLVGVVSAVVAGARDKALAGLLFYLAAYTATVIGAFAVVGALERRTRGDFEP
HHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ADAWDLDRFAGLARRRPALAFAMAVFLFSLAGVPPTAGFIGKFYIFKAAMGAGLYGLAVL
CCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHH
GVLTSVLGAYYYLRVVLYMYFLPSKDEAGEVVLSAPSLTIALAAAVAVVVLLGVVADPMV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
RLAQAASAIIL
HHHHHHHHHCH
>Mature Secondary Structure 
SGFPTQDFVALVPVAILTMGALVLLMTEVFLTSGRRAYQAYLTMVFAAAAAGYAAWMPV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEECCC
PGNVFGRQAVVDSFSAFVTVVLCAGLALSALVGQSWLNARNSERGEFYALALFGTAGMVL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHH
LGMATDLLIAFIAVEVMSLSTYCLAAFLRRGKKPAEAAFKYVVLGSISSALLLYGSALLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GASGSTLFSVLPRGQGSALYLAGIALVAGGVAFKIAAVPFHAWTPDVYEGAPTPVTAFMA
CCCCCHHHHHCCCCCCCCHHHHHHHHHHCCHHHEEEEECCCCCCCCCCCCCCCHHHHHHH
AGVKTAAFAVLVRLFLATESGSAAMSTSLGTVLSALAVLTMIFGNLLALPQRSVKRMLAY
HCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
SSIGHAGYLLVGVVSAVVAGARDKALAGLLFYLAAYTATVIGAFAVVGALERRTRGDFEP
HHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ADAWDLDRFAGLARRRPALAFAMAVFLFSLAGVPPTAGFIGKFYIFKAAMGAGLYGLAVL
CCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHH
GVLTSVLGAYYYLRVVLYMYFLPSKDEAGEVVLSAPSLTIALAAAVAVVVLLGVVADPMV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
RLAQAASAIIL
HHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA