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The map label for this gene is gluQ [H]

Identifier: 220915438

GI number: 220915438

Start: 355006

End: 355977

Strand: Direct

Name: gluQ [H]

Synonym: A2cp1_0317

Alternate gene names: 220915438

Gene position: 355006-355977 (Clockwise)

Preceding gene: 220915431

Following gene: 220915439

Centisome position: 7.06

GC content: 79.42

Gene sequence:

>972_bases
GTGTCCAGCCGCGGCCGCTTCGCTCCCTCCCCCACCGGACCCCTGCACCTCGGCAACGCGCGCACCGCGCTCCTGTCGTG
GCTCGCCGCGCGCACCGCCGGGGCGGCCTACGTCATGCGGGTGGAGGACCTGGACGGCCCGCGCGTGCGGCCCGGCCTGG
AGGCGCGGATCCTGGAGGAGCTGCGCTGGCTCGGCCTGGACTGGGACGAGGGGCCGGACGTGGGCGGGCCGCTCGGTCCC
TACCGCCAGTCGGAGCGCCTGCCGCGGTACGGCGCCGCGCTGGAGCGGCTCCGCGCCGCAGGGCTCGCCTACCCGTGCTT
CTGCTCGCGCGCCGAGATCGCGGCGGCCTCGCAGGCGCCGCACGGCGCCTCGGACGAGGGGCCGCGGTACCCGGGCACCT
GTCGCGAGCTCTCCCCCGCAGAGGTGGCCAGGCGCGCCGCGACGCGCCGGCCGGCCTGGCGGCTGCGCGTCGCGGCGGGC
CCGGTCGCCTTCGAGGACGGCGTCCACGGCCCGGTGGCGCACGACGTCGCGGCGGAGGTGGGCGACTTCGTGATCGCGCG
GGCCGACGGCGTGCCGGCGTACCAGCTCGCGGTGGTGGTCGACGACGCGGCCATGCAGGTGGGGGAGGTGGTCCGCGGCG
ACGACCTCCTGCCGTCCACCGCGCGCCAGCTCCTGCTGTACCGCGCGCTGGGCGTCGCCCCGCCGCGCTTCGCGCACGTG
CCGCTCGTGGTCGGCCCCGACGGCGCGCGGCTCGCGAAGCGGCACGGCGCGCTCTCGCTGGGCGAGCTCCGGGCGCGCGG
CGCGGACCCGCGCGCGGTGGTCGCGCTGCTCGCCGGGCTCTCCGGCCTGGCGGCCGCCGACCCGCACGCCGCGTGTACCC
CTCGGGAGCTGGTCGGCCGCTTCTCGATGGCGCGGCTGCCGCGCGAACCCGCCGTCCTCTCCCCGGAACGGATCGCGGCG
CTCCTCCCGTGA

Upstream 100 bases:

>100_bases
TTCGGCGCGCGGGGGCCAAGGGCAGCGGATATCATCGCAGCGCCCAACCGGCACCGCCCAACCGGCACCGCCCAACCGGC
ACCGACCTCCCGGGGCACCC

Downstream 100 bases:

>100_bases
TCGCGTACACTGCGCGCGTGCGCCTGCTCCGCTCTCCCACCGACCCGCGCTGGATCGACGTCGCGCTCGCGGAGCTGGAT
CGCACGCTGCAGGACCACGC

Product: Glutamate--tRNA ligase

Products: AMP; diphosphate; L-glutamyl-tRNA(Glu)

Alternate protein names: Glu-Q-RSs [H]

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MSSRGRFAPSPTGPLHLGNARTALLSWLAARTAGAAYVMRVEDLDGPRVRPGLEARILEELRWLGLDWDEGPDVGGPLGP
YRQSERLPRYGAALERLRAAGLAYPCFCSRAEIAAASQAPHGASDEGPRYPGTCRELSPAEVARRAATRRPAWRLRVAAG
PVAFEDGVHGPVAHDVAAEVGDFVIARADGVPAYQLAVVVDDAAMQVGEVVRGDDLLPSTARQLLLYRALGVAPPRFAHV
PLVVGPDGARLAKRHGALSLGELRARGADPRAVVALLAGLSGLAAADPHAACTPRELVGRFSMARLPREPAVLSPERIAA
LLP

Sequences:

>Translated_323_residues
MSSRGRFAPSPTGPLHLGNARTALLSWLAARTAGAAYVMRVEDLDGPRVRPGLEARILEELRWLGLDWDEGPDVGGPLGP
YRQSERLPRYGAALERLRAAGLAYPCFCSRAEIAAASQAPHGASDEGPRYPGTCRELSPAEVARRAATRRPAWRLRVAAG
PVAFEDGVHGPVAHDVAAEVGDFVIARADGVPAYQLAVVVDDAAMQVGEVVRGDDLLPSTARQLLLYRALGVAPPRFAHV
PLVVGPDGARLAKRHGALSLGELRARGADPRAVVALLAGLSGLAAADPHAACTPRELVGRFSMARLPREPAVLSPERIAA
LLP
>Mature_322_residues
SSRGRFAPSPTGPLHLGNARTALLSWLAARTAGAAYVMRVEDLDGPRVRPGLEARILEELRWLGLDWDEGPDVGGPLGPY
RQSERLPRYGAALERLRAAGLAYPCFCSRAEIAAASQAPHGASDEGPRYPGTCRELSPAEVARRAATRRPAWRLRVAAGP
VAFEDGVHGPVAHDVAAEVGDFVIARADGVPAYQLAVVVDDAAMQVGEVVRGDDLLPSTARQLLLYRALGVAPPRFAHVP
LVVGPDGARLAKRHGALSLGELRARGADPRAVVALLAGLSGLAAADPHAACTPRELVGRFSMARLPREPAVLSPERIAAL
LP

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=318, Percent_Identity=41.8238993710692, Blast_Score=238, Evalue=7e-63,
Organism=Escherichia coli, GI1788743, Length=321, Percent_Identity=36.4485981308411, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI226510925, Length=281, Percent_Identity=39.1459074733096, Blast_Score=157, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI71993394, Length=318, Percent_Identity=33.6477987421384, Blast_Score=186, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6324540, Length=330, Percent_Identity=33.030303030303, Blast_Score=183, Evalue=3e-47,
Organism=Drosophila melanogaster, GI24665321, Length=325, Percent_Identity=38.1538461538462, Blast_Score=232, Evalue=2e-61,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR022380
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: 6.1.1.17

Molecular weight: Translated: 34115; Mature: 33984

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSRGRFAPSPTGPLHLGNARTALLSWLAARTAGAAYVMRVEDLDGPRVRPGLEARILEE
CCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHH
LRWLGLDWDEGPDVGGPLGPYRQSERLPRYGAALERLRAAGLAYPCFCSRAEIAAASQAP
HHHCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCHHHCCCHHHHHHCCCC
HGASDEGPRYPGTCRELSPAEVARRAATRRPAWRLRVAAGPVAFEDGVHGPVAHDVAAEV
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCEEECCCCCCCHHHHHHHHH
GDFVIARADGVPAYQLAVVVDDAAMQVGEVVRGDDLLPSTARQLLLYRALGVAPPRFAHV
HCEEEEECCCCCEEEEEEEECHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEE
PLVVGPDGARLAKRHGALSLGELRARGADPRAVVALLAGLSGLAAADPHAACTPRELVGR
CEEECCCCHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
FSMARLPREPAVLSPERIAALLP
HHHHHCCCCCCCCCHHHHHHCCC
>Mature Secondary Structure 
SSRGRFAPSPTGPLHLGNARTALLSWLAARTAGAAYVMRVEDLDGPRVRPGLEARILEE
CCCCCCCCCCCCCEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHH
LRWLGLDWDEGPDVGGPLGPYRQSERLPRYGAALERLRAAGLAYPCFCSRAEIAAASQAP
HHHCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCHHHCCCHHHHHHCCCC
HGASDEGPRYPGTCRELSPAEVARRAATRRPAWRLRVAAGPVAFEDGVHGPVAHDVAAEV
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCEEECCCCCCCHHHHHHHHH
GDFVIARADGVPAYQLAVVVDDAAMQVGEVVRGDDLLPSTARQLLLYRALGVAPPRFAHV
HCEEEEECCCCCEEEEEEEECHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEE
PLVVGPDGARLAKRHGALSLGELRARGADPRAVVALLAGLSGLAAADPHAACTPRELVGR
CEEECCCCHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
FSMARLPREPAVLSPERIAALLP
HHHHHCCCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; tRNA(Glu)

Specific reaction: ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA