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The map label for this gene is aroA

Identifier: 220915327

GI number: 220915327

Start: 225687

End: 227009

Strand: Direct

Name: aroA

Synonym: A2cp1_0206

Alternate gene names: 220915327

Gene position: 225687-227009 (Clockwise)

Preceding gene: 220915326

Following gene: 220915328

Centisome position: 4.49

GC content: 75.89

Gene sequence:

>1323_bases
TTGAGCGCTGCCCCGACCTCCGGACCCCTGACCTGCCGGCGCGCCGGCCCGCTGCGCGGCGCCATCGAGGTGCCCGGCGA
CAAGTCCATCTCGCACCGGAGCCTGCTCTTCGGCGCGCTGTCCACCGGCGAGACCCGCGTCGCGGGGCTGCTCGACGCCG
AGGACGTCCACTCCACCCGCAGGGCGGTGGAGGCGCTCGGCGCCACGGTGCGCACGGAGGGCGCCGAGGTGGTGGTGACG
CCGCCCGCGACGCTGCGCGAGCCGGGGGACGTCATCGACTGCGGCAACTCCGGCACGAGCCTGCGCCTGCTCACCGGCGT
GCTCTCCGGCGTGCCCGGCCTGTCGGTGCTCACCGGCGACGCCTCGCTGCGGCGCCGCCCGGTGCGGCGGGTGATCGACC
CGCTCCGGGTGATGGGCGCGAACCTGTCCGCCCGCGACGGCGATCGCCTGCCGCCGGTGGTCGTCCGCGGGGGCGCGCTG
CGCGGGGCGCGGCACCTGCTCCCGGTGGCGAGCGCGCAGGTGAAGAGCGCGCTGCTGCTCGCCGGCCTGTTCGCCGAGGG
CGAGACCACGGTGGTCGAGCCGGAGAAGAGCCGCGATCACACCGAGCGCATGCTGCGCGGGATGGGCGTGCCGGTGAAGG
TCTCGGGCCTGGAGGTGTCGGTCTCGGCAGCGCGGCCCGCCGGCGGGCGCGTGGACGTGCCGGGTGACATCTCCTCGGCG
GCGTTCTTCCTCTGCGGCGCGGCGGCGCTCCCCGGCTCCGAGGTGACGGTCCGCAACCTGGGCGTGAACGAGACGCGCAC
CGGGCTGCTCGACGTGCTCCGGGCCATGGGCGCCGACGTGCGGCTCGCGAACCTCCGCGAGGTGGCGGGCGAGCCGCGCG
CCGACGTGACCGTGCGCGCCGATCGCCTGGAGGGCACCGAGATCCGCGGCGCGACCATCCCCCGGCTCATCGACGAGCTG
CCGGTGGTGATGGTGATGGCCACGCAGGCGCGCGGCCGGACCGTGATCCGCGACGCGAAGGAGCTCCGGGTGAAGGAGTC
CGACCGGCTCGCCGCCATGGGCGAGACGCTCTCGCGCGCCGGCGCCCGCATCGAGCTGTTCGAGGACGGCTGCGCCATCG
ACGGGCCCACGCCGCTGCGCGGCGTCGAGGTCCGCACCCGGCTGGATCACCGCATCGCCATGTCCATGGCGGTCGCGCAG
CTGTTCTGCGGCGGGGAGCCGGTGGTGCTCGACGACGTGGCCTGCGTCGCGACCAGCTTCCCGAGCTTCTTCCGGCTGCT
CGACCAGGTGTCCGGCCAGGCGTCCGGCGGAGGCGCGCCGTGA

Upstream 100 bases:

>100_bases
TGGAGCGGCACCTCGCCCGCCTCCTCGCCGCCATCGCCGAGTCGCCGGAGTCGGCGCGCTCGGCCCTCGAGGCGGCCCGC
GCCGCACGGGAGGAGCTGTT

Downstream 100 bases:

>100_bases
TCACCGGGCGGACCGCGCTCTACGGCGTCGTCGGCCACCCGGTGGCGCACAGCCGCTCGCCGGAGATGCAGAACGCGGCG
TTCGCGAGGCTGGGCGTGGA

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MSAAPTSGPLTCRRAGPLRGAIEVPGDKSISHRSLLFGALSTGETRVAGLLDAEDVHSTRRAVEALGATVRTEGAEVVVT
PPATLREPGDVIDCGNSGTSLRLLTGVLSGVPGLSVLTGDASLRRRPVRRVIDPLRVMGANLSARDGDRLPPVVVRGGAL
RGARHLLPVASAQVKSALLLAGLFAEGETTVVEPEKSRDHTERMLRGMGVPVKVSGLEVSVSAARPAGGRVDVPGDISSA
AFFLCGAAALPGSEVTVRNLGVNETRTGLLDVLRAMGADVRLANLREVAGEPRADVTVRADRLEGTEIRGATIPRLIDEL
PVVMVMATQARGRTVIRDAKELRVKESDRLAAMGETLSRAGARIELFEDGCAIDGPTPLRGVEVRTRLDHRIAMSMAVAQ
LFCGGEPVVLDDVACVATSFPSFFRLLDQVSGQASGGGAP

Sequences:

>Translated_440_residues
MSAAPTSGPLTCRRAGPLRGAIEVPGDKSISHRSLLFGALSTGETRVAGLLDAEDVHSTRRAVEALGATVRTEGAEVVVT
PPATLREPGDVIDCGNSGTSLRLLTGVLSGVPGLSVLTGDASLRRRPVRRVIDPLRVMGANLSARDGDRLPPVVVRGGAL
RGARHLLPVASAQVKSALLLAGLFAEGETTVVEPEKSRDHTERMLRGMGVPVKVSGLEVSVSAARPAGGRVDVPGDISSA
AFFLCGAAALPGSEVTVRNLGVNETRTGLLDVLRAMGADVRLANLREVAGEPRADVTVRADRLEGTEIRGATIPRLIDEL
PVVMVMATQARGRTVIRDAKELRVKESDRLAAMGETLSRAGARIELFEDGCAIDGPTPLRGVEVRTRLDHRIAMSMAVAQ
LFCGGEPVVLDDVACVATSFPSFFRLLDQVSGQASGGGAP
>Mature_439_residues
SAAPTSGPLTCRRAGPLRGAIEVPGDKSISHRSLLFGALSTGETRVAGLLDAEDVHSTRRAVEALGATVRTEGAEVVVTP
PATLREPGDVIDCGNSGTSLRLLTGVLSGVPGLSVLTGDASLRRRPVRRVIDPLRVMGANLSARDGDRLPPVVVRGGALR
GARHLLPVASAQVKSALLLAGLFAEGETTVVEPEKSRDHTERMLRGMGVPVKVSGLEVSVSAARPAGGRVDVPGDISSAA
FFLCGAAALPGSEVTVRNLGVNETRTGLLDVLRAMGADVRLANLREVAGEPRADVTVRADRLEGTEIRGATIPRLIDELP
VVMVMATQARGRTVIRDAKELRVKESDRLAAMGETLSRAGARIELFEDGCAIDGPTPLRGVEVRTRLDHRIAMSMAVAQL
FCGGEPVVLDDVACVATSFPSFFRLLDQVSGQASGGGAP

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family

Homologues:

Organism=Escherichia coli, GI1787137, Length=441, Percent_Identity=30.1587301587302, Blast_Score=154, Evalue=7e-39,
Organism=Saccharomyces cerevisiae, GI6320332, Length=469, Percent_Identity=27.2921108742004, Blast_Score=120, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROA_ANAD2 (B8J8W7)

Other databases:

- EMBL:   CP001359
- RefSeq:   YP_002490631.1
- ProteinModelPortal:   B8J8W7
- SMR:   B8J8W7
- GeneID:   7298441
- GenomeReviews:   CP001359_GR
- KEGG:   acp:A2cp1_0206
- HOGENOM:   HBG646626
- ProtClustDB:   PRK02427
- GO:   GO:0005737
- HAMAP:   MF_00210
- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.10
- PIRSF:   PIRSF000505
- TIGRFAMs:   TIGR01356

Pfam domain/function: PF00275 EPSP_synthase; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =2.5.1.19

Molecular weight: Translated: 45931; Mature: 45800

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: PS00104 EPSP_SYNTHASE_1; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAPTSGPLTCRRAGPLRGAIEVPGDKSISHRSLLFGALSTGETRVAGLLDAEDVHSTR
CCCCCCCCCEEEECCCCCCCEEECCCCCCCCHHHHEEEECCCCCCCEEEECCHHHHHHHH
RAVEALGATVRTEGAEVVVTPPATLREPGDVIDCGNSGTSLRLLTGVLSGVPGLSVLTGD
HHHHHHCCCEECCCCEEEECCCHHHCCCCCEEECCCCCCHHHHHHHHHHCCCCCEEEECC
ASLRRRPVRRVIDPLRVMGANLSARDGDRLPPVVVRGGALRGARHLLPVASAQVKSALLL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHH
AGLFAEGETTVVEPEKSRDHTERMLRGMGVPVKVSGLEVSVSAARPAGGRVDVPGDISSA
HHHHCCCCEEEECCCCCHHHHHHHHHHCCCCEEECCEEEEEEECCCCCCEECCCCCHHHH
AFFLCGAAALPGSEVTVRNLGVNETRTGLLDVLRAMGADVRLANLREVAGEPRADVTVRA
HHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEHHHHHHCCCCCCCEEEEE
DRLEGTEIRGATIPRLIDELPVVMVMATQARGRTVIRDAKELRVKESDRLAAMGETLSRA
CCCCCCEECCCHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHC
GARIELFEDGCAIDGPTPLRGVEVRTRLDHRIAMSMAVAQLFCGGEPVVLDDVACVATSF
CCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCC
PSFFRLLDQVSGQASGGGAP
HHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SAAPTSGPLTCRRAGPLRGAIEVPGDKSISHRSLLFGALSTGETRVAGLLDAEDVHSTR
CCCCCCCCEEEECCCCCCCEEECCCCCCCCHHHHEEEECCCCCCCEEEECCHHHHHHHH
RAVEALGATVRTEGAEVVVTPPATLREPGDVIDCGNSGTSLRLLTGVLSGVPGLSVLTGD
HHHHHHCCCEECCCCEEEECCCHHHCCCCCEEECCCCCCHHHHHHHHHHCCCCCEEEECC
ASLRRRPVRRVIDPLRVMGANLSARDGDRLPPVVVRGGALRGARHLLPVASAQVKSALLL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHH
AGLFAEGETTVVEPEKSRDHTERMLRGMGVPVKVSGLEVSVSAARPAGGRVDVPGDISSA
HHHHCCCCEEEECCCCCHHHHHHHHHHCCCCEEECCEEEEEEECCCCCCEECCCCCHHHH
AFFLCGAAALPGSEVTVRNLGVNETRTGLLDVLRAMGADVRLANLREVAGEPRADVTVRA
HHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEHHHHHHCCCCCCCEEEEE
DRLEGTEIRGATIPRLIDELPVVMVMATQARGRTVIRDAKELRVKESDRLAAMGETLSRA
CCCCCCEECCCHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHC
GARIELFEDGCAIDGPTPLRGVEVRTRLDHRIAMSMAVAQLFCGGEPVVLDDVACVATSF
CCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCC
PSFFRLLDQVSGQASGGGAP
HHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA