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The map label for this gene is mcl2 [H]

Identifier: 220915324

GI number: 220915324

Start: 223476

End: 224369

Strand: Direct

Name: mcl2 [H]

Synonym: A2cp1_0203

Alternate gene names: 220915324

Gene position: 223476-224369 (Clockwise)

Preceding gene: 220915322

Following gene: 220915325

Centisome position: 4.44

GC content: 79.87

Gene sequence:

>894_bases
ATGCCCGTCGTCCGCCCCCGCCGCAGCGCCCTGTACCTGCCCGGCTCCAACGCGCGCGCCCTCGAGAAGGCGCGCACGCT
CCCCGCCGACGCGCTCCTCCTCGACCTGGAGGACGCGGTGGCGCCCGCCGCCAAGGACGCCGCCCGCGCGCAGGTGGTGG
CCGCGCTGGCGCAGGGCGGCTTCGGCCGGCGCGAGCGCGTGGTGCGCGTGAACGGGCTCGGGACGCCGTGGGGCGCGGCC
GATCTGGCGGCGCTGGCCCGCGCCGGCGCCGACGCGATCTGCCTGCCGAAGGTGGAGCGCGCCGCCGAGGTGCACGCCGC
GGTGCAGGCGCTCGCGGCCGGCCACGCGCCGGCCGGCCTCGCGCTCTGGTGCATGGTCGAGACCCCGCGCGGCGTGCTGG
CGGCCGACGAGATCGCCGGCGCGTCGCCGAGGGTGGCGTGCCTGGTGGCCGGCACGAGCGACCTCGTGAAGGACCTGGGC
GCGCGCCACACCGCCGGCCGCGCCGAGGTCCTCACCAGCCTGTCGCTCGTCCTGCTCGCCGCGCGCGCCCACGGGCTCGC
CGCGCTCGACGGCGTGTTCCTCGACCTCGAGGACGCGGCGGGGCTGGAGGCCGCCTGCCGGCAGGGGCGGGACCTCGGCT
TCGACGGCAAGACGCTCATCCACCCGAAGCAGCTCGAGGCGGCGAACCGCGCCTTCGCGCCGGACGACGCCGAGCTGGAG
CGGGCGCGGCGGGTGATCGCGGCGCACGCCGAGGCCGAGGCGGCCGGGCTGGGCGTCACGGTGGTGGACGGGCGCCTGGT
GGAGGCGCTGCACGTCGCGGCGGCGCGCCGGACGGTGGCGCTCGCCGAGGCCGTCGCGGCCGGGCATGGCGGGCCGGACC
CGCACGGCGGGTGA

Upstream 100 bases:

>100_bases
AGGATCGTGACGGCGGGCGGGGGCGACAACCGCATCCCCGCTTCCGCGGGGCGGCCCGCTCTGGCACGCTCGCCTCGTTC
GTCCCGCCGAGGAGCCCTCG

Downstream 100 bases:

>100_bases
TCCAAGAGCGCGTGGATCCGGCGGAAAGCGGGTGGACACCCGCGCGCCCGTTTCCTACACTGTTCGACTCCCATGCGTGG
CATTCGAGGCGCGACCCAGT

Product: Citrate (pro-3S)-lyase

Products: acetate; oxaloacetate

Alternate protein names: (3S)-malyl-CoA thioesterase [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MPVVRPRRSALYLPGSNARALEKARTLPADALLLDLEDAVAPAAKDAARAQVVAALAQGGFGRRERVVRVNGLGTPWGAA
DLAALARAGADAICLPKVERAAEVHAAVQALAAGHAPAGLALWCMVETPRGVLAADEIAGASPRVACLVAGTSDLVKDLG
ARHTAGRAEVLTSLSLVLLAARAHGLAALDGVFLDLEDAAGLEAACRQGRDLGFDGKTLIHPKQLEAANRAFAPDDAELE
RARRVIAAHAEAEAAGLGVTVVDGRLVEALHVAAARRTVALAEAVAAGHGGPDPHGG

Sequences:

>Translated_297_residues
MPVVRPRRSALYLPGSNARALEKARTLPADALLLDLEDAVAPAAKDAARAQVVAALAQGGFGRRERVVRVNGLGTPWGAA
DLAALARAGADAICLPKVERAAEVHAAVQALAAGHAPAGLALWCMVETPRGVLAADEIAGASPRVACLVAGTSDLVKDLG
ARHTAGRAEVLTSLSLVLLAARAHGLAALDGVFLDLEDAAGLEAACRQGRDLGFDGKTLIHPKQLEAANRAFAPDDAELE
RARRVIAAHAEAEAAGLGVTVVDGRLVEALHVAAARRTVALAEAVAAGHGGPDPHGG
>Mature_296_residues
PVVRPRRSALYLPGSNARALEKARTLPADALLLDLEDAVAPAAKDAARAQVVAALAQGGFGRRERVVRVNGLGTPWGAAD
LAALARAGADAICLPKVERAAEVHAAVQALAAGHAPAGLALWCMVETPRGVLAADEIAGASPRVACLVAGTSDLVKDLGA
RHTAGRAEVLTSLSLVLLAARAHGLAALDGVFLDLEDAAGLEAACRQGRDLGFDGKTLIHPKQLEAANRAFAPDDAELER
ARRVIAAHAEAEAAGLGVTVVDGRLVEALHVAAARRTVALAEAVAAGHGGPDPHGG

Specific function: Catalyzes the hydrolysis of (3S)-malyl-CoA to (3S)- malate and free CoA. Inactive towards beta-methylmalyl-CoA and other CoA esters [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=294, Percent_Identity=30.6122448979592, Blast_Score=141, Evalue=7e-34,
Organism=Escherichia coli, GI87081764, Length=285, Percent_Identity=37.5438596491228, Blast_Score=169, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17557458, Length=301, Percent_Identity=27.5747508305648, Blast_Score=109, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6

Molecular weight: Translated: 30196; Mature: 30065

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVVRPRRSALYLPGSNARALEKARTLPADALLLDLEDAVAPAAKDAARAQVVAALAQGG
CCCCCCCCCEEEECCCCHHHHHHHHCCCHHHEEEEHHHHHCHHHHHHHHHHHHHHHHHCC
FGRRERVVRVNGLGTPWGAADLAALARAGADAICLPKVERAAEVHAAVQALAAGHAPAGL
CCCCCEEEEEECCCCCCCHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHCCCCCCCE
ALWCMVETPRGVLAADEIAGASPRVACLVAGTSDLVKDLGARHTAGRAEVLTSLSLVLLA
EEEEEEECCCCCEEHHHHCCCCCCEEEEEECCHHHHHHHCCCCCCCHHHHHHHHHHHHHH
ARAHGLAALDGVFLDLEDAAGLEAACRQGRDLGFDGKTLIHPKQLEAANRAFAPDDAELE
HHHCCCHHHCCEEEECCCCCCHHHHHHCCCCCCCCCCEECCHHHHHHHCCCCCCCHHHHH
RARRVIAAHAEAEAAGLGVTVVDGRLVEALHVAAARRTVALAEAVAAGHGGPDPHGG
HHHHHHHHHCCHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PVVRPRRSALYLPGSNARALEKARTLPADALLLDLEDAVAPAAKDAARAQVVAALAQGG
CCCCCCCCEEEECCCCHHHHHHHHCCCHHHEEEEHHHHHCHHHHHHHHHHHHHHHHHCC
FGRRERVVRVNGLGTPWGAADLAALARAGADAICLPKVERAAEVHAAVQALAAGHAPAGL
CCCCCEEEEEECCCCCCCHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHCCCCCCCE
ALWCMVETPRGVLAADEIAGASPRVACLVAGTSDLVKDLGARHTAGRAEVLTSLSLVLLA
EEEEEEECCCCCEEHHHHCCCCCCEEEEEECCHHHHHHHCCCCCCCHHHHHHHHHHHHHH
ARAHGLAALDGVFLDLEDAAGLEAACRQGRDLGFDGKTLIHPKQLEAANRAFAPDDAELE
HHHCCCHHHCCEEEECCCCCCHHHHHHCCCCCCCCCCEECCHHHHHHHCCCCCCCHHHHH
RARRVIAAHAEAEAAGLGVTVVDGRLVEALHVAAARRTVALAEAVAAGHGGPDPHGG
HHHHHHHHHCCHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0565 {oxaoacetate}} 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA