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The map label for this gene is bioD

Identifier: 220915209

GI number: 220915209

Start: 100057

End: 100728

Strand: Direct

Name: bioD

Synonym: A2cp1_0088

Alternate gene names: 220915209

Gene position: 100057-100728 (Clockwise)

Preceding gene: 220915208

Following gene: 220915210

Centisome position: 1.99

GC content: 77.38

Gene sequence:

>672_bases
GTGCGCGGGCTGTTCGTCACCGGCACCGACACCGGCGTGGGCAAGACCGAGGTGGCGTGCGCGCTGGTGCGCGCCGCGCG
CGCCGCCGGCCTGGACGCGGTGGGCATGAAGCCGGCGCAGTCCGGCCACGTGCCCGGCGAGCCGTCCGACGCGGAGCGGC
TCCGCGAGGCGTCGGACCGCGTCGAGCCGCTCGAGGCGATCTGCCCGTACACGTTCGGTGCCCCGCTCGCGCCGGCCGCC
GCGGCGCGGCTGGAGGGGCGCGAGGTCTCGCTGGCGCGGGTGGTGGAGGCGGCGCGCGCGCTCGCGGCGCGCCACGCCGC
GGTGGTGGTGGAGGGGGCCGGCGGGCTGCTCGTGCCGCTCACCGCGCGCGAGACCCACGCCGACCTCGCCGCGGCGCTGG
GCCTGCCGGTGCTGGTGGTGGCGCGCGCCGGGCTCGGGACGGTGAACCACACCGCGCTCACCGTCGAGGCGCTGGAGCGC
CGCGGCCTCGCGGTGGCCGGGATCGTGCTCAACCGGACCGGGCCCGAGGACGATCCCTCGGTGCCGCTCAACGCCGCGGA
GATCGCGCGCTTGACGTACCGTGAACCACTCGCGCTGCTGCCGTGGGAGCCCGATATCGCGCGGCGCGCGCGCTCTCTCG
GATCCATCCTCGGCGCGAAGATCCAGTTTTGA

Upstream 100 bases:

>100_bases
TGGTGGTGCTCATGCCGCCGCTCGCCATGACCGAGGCCGAGCTCACGCGGCTGGCCGGGATCACGCTCGCCGCCATCGAG
GCGGCCACCGCGAGGCTCTA

Downstream 100 bases:

>100_bases
GCGGTGGGAGCGAAAATTGGATCGATCGCGAGATCGATCCTAGATCGCTGACGTGCCGAACGTCGTCGACCTCACGCTCG
TCTCGCGCGCCCGGCGCGCG

Product: dethiobiotin synthase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MRGLFVTGTDTGVGKTEVACALVRAARAAGLDAVGMKPAQSGHVPGEPSDAERLREASDRVEPLEAICPYTFGAPLAPAA
AARLEGREVSLARVVEAARALAARHAAVVVEGAGGLLVPLTARETHADLAAALGLPVLVVARAGLGTVNHTALTVEALER
RGLAVAGIVLNRTGPEDDPSVPLNAAEIARLTYREPLALLPWEPDIARRARSLGSILGAKIQF

Sequences:

>Translated_223_residues
MRGLFVTGTDTGVGKTEVACALVRAARAAGLDAVGMKPAQSGHVPGEPSDAERLREASDRVEPLEAICPYTFGAPLAPAA
AARLEGREVSLARVVEAARALAARHAAVVVEGAGGLLVPLTARETHADLAAALGLPVLVVARAGLGTVNHTALTVEALER
RGLAVAGIVLNRTGPEDDPSVPLNAAEIARLTYREPLALLPWEPDIARRARSLGSILGAKIQF
>Mature_223_residues
MRGLFVTGTDTGVGKTEVACALVRAARAAGLDAVGMKPAQSGHVPGEPSDAERLREASDRVEPLEAICPYTFGAPLAPAA
AARLEGREVSLARVVEAARALAARHAAVVVEGAGGLLVPLTARETHADLAAALGLPVLVVARAGLGTVNHTALTVEALER
RGLAVAGIVLNRTGPEDDPSVPLNAAEIARLTYREPLALLPWEPDIARRARSLGSILGAKIQF

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family

Homologues:

Organism=Escherichia coli, GI1786995, Length=175, Percent_Identity=40, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI87081944, Length=179, Percent_Identity=30.1675977653631, Blast_Score=68, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324385, Length=181, Percent_Identity=38.6740331491713, Blast_Score=85, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOD_ANAD2 (B8J7V5)

Other databases:

- EMBL:   CP001359
- RefSeq:   YP_002490513.1
- ProteinModelPortal:   B8J7V5
- SMR:   B8J7V5
- GeneID:   7299730
- GenomeReviews:   CP001359_GR
- KEGG:   acp:A2cp1_0088
- HOGENOM:   HBG650065
- ProtClustDB:   CLSK944803
- HAMAP:   MF_00336
- InterPro:   IPR004472
- InterPro:   IPR002586
- PIRSF:   PIRSF006755
- TIGRFAMs:   TIGR00347

Pfam domain/function: PF01656 CbiA

EC number: =6.3.3.3

Molecular weight: Translated: 23002; Mature: 23002

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGLFVTGTDTGVGKTEVACALVRAARAAGLDAVGMKPAQSGHVPGEPSDAERLREASDR
CCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
VEPLEAICPYTFGAPLAPAAAARLEGREVSLARVVEAARALAARHAAVVVEGAGGLLVPL
CCHHHHHCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEE
TARETHADLAAALGLPVLVVARAGLGTVNHTALTVEALERRGLAVAGIVLNRTGPEDDPS
CCCHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCC
VPLNAAEIARLTYREPLALLPWEPDIARRARSLGSILGAKIQF
CCCCHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRGLFVTGTDTGVGKTEVACALVRAARAAGLDAVGMKPAQSGHVPGEPSDAERLREASDR
CCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
VEPLEAICPYTFGAPLAPAAAARLEGREVSLARVVEAARALAARHAAVVVEGAGGLLVPL
CCHHHHHCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEE
TARETHADLAAALGLPVLVVARAGLGTVNHTALTVEALERRGLAVAGIVLNRTGPEDDPS
CCCHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCC
VPLNAAEIARLTYREPLALLPWEPDIARRARSLGSILGAKIQF
CCCCHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA