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The map label for this gene is bioK [H]

Identifier: 220915208

GI number: 220915208

Start: 98684

End: 100057

Strand: Direct

Name: bioK [H]

Synonym: A2cp1_0087

Alternate gene names: 220915208

Gene position: 98684-100057 (Clockwise)

Preceding gene: 220915207

Following gene: 220915209

Centisome position: 1.96

GC content: 74.02

Gene sequence:

>1374_bases
ATGGACGACCGCGAACGCCGTGAGCGGCACCGCCGGCTGTGGGAGGCGGACCACCGGCACCTCTGGCACCCGTTCACGCA
GATGAGGGACTGGCTCCGCGAGGAGCCGCTCATCGTCGACGCGGCCGAGGGCGTCCACCTCGTGGACACGCTCGGGAACC
GCTACCTCGACGGCGTGTCGTCGCTCTGGTGCAACGTGCACGGCCACCGCGTCCCGGAGCTCGACGCCGCGGTGCGCGCC
CAGCTCGAACGCGTGGCGCACTCGACGCTGCTCGGCCTCGCCTCCACCGCGTCGATCGAGTGCGCCGAGGAGCTGGTCCG
CCACCTGCCGTCCGGCCTGACCCGGGTCTTCTTCTCCGACGCGGGCGCCACCGCGGTGGAGATCGCCATCAAGATGGCGT
TCCAGCACCACCAGCTCCGCGGCGACGAGCGCCGCACCGAGTTCGTGGCGCTGCACGGCGGCTACCACGGCGACACCCTC
GGCTCGGTCTCGGTGGGCGGCATCGACCTGTTCCACCGCATCTTCAAGCCGCTGCTGTTCGACGTGCACCACGCGCCGCA
GCCCTACTGCTACCGCTGCCCGCTCGGGAAGGCGCTCCCCGGCTGCGGGATGGCCTGCGCGGACGAGGTGGAGCGCATCC
TCGCGGCGCGCGAGGGCAGGGTGGCCGCGCTCGTGGTCGAGCCGCTCGTGCAGGGCGCGGACGGCATGATCACCCAGCCG
CCCGGGTACCTGCGCCGCCTGCGCGAGCTCTGCGACCGCCACGGCGCGCTGCTGGTCTGCGACGAGGTCGCGACCGGCTG
GGGCCGCACCGGGACCACGTTCGCGGTGGAGCAGGAGGGCGTCCTGCCGGACATCCTCACCATGGCGAAGGGGCTCACCG
GCGGCTACCTGCCGCTCGCCGCCACCGTCACCACCGAGCAGGTCTTCGAGTCGTTCCTGGGCGAGCACGCCGAGAAGCGG
ACCTTCTTCCACGGCCACACCTACGCCGGGAACCCGCTCGCCTGCGCCGCCGCGACGGCCTCCATGACGCTGTTCCGCGA
GCGCGGCGTGGGCGCGGCGGTGCCGGCGAAGGCGGCCGCGCTCGCCCGCGCGCTCGCGCCGGCGGCGCGGCTCCCGCACG
TGGGCGAGGTCCGGCAGCGCGGGCTGATGGTGGGCATCGAGCTGGTGCGCGACCGGGCCACGAAGGAGGAGTACGCGTAC
GCGCTGCAGGCCGGGCACCGGGTGATCCTGGAGGCCCGCAAGCTCGGCGCGATCCTCCGGCCGCTCGGCAACGTGGTGGT
GCTCATGCCGCCGCTCGCCATGACCGAGGCCGAGCTCACGCGGCTGGCCGGGATCACGCTCGCCGCCATCGAGGCGGCCA
CCGCGAGGCTCTAG

Upstream 100 bases:

>100_bases
CGGGCGCTCGGCGCGCGCCTGCAGGGCCGCCGCCCCCCCGCGCCGCCCGCGGCCGCCCGGCCGTGACGGCGCCGGGCCGG
TGCGCTAGGGTCGCGGCCCT

Downstream 100 bases:

>100_bases
TGCGCGGGCTGTTCGTCACCGGCACCGACACCGGCGTGGGCAAGACCGAGGTGGCGTGCGCGCTGGTGCGCGCCGCGCGC
GCCGCCGGCCTGGACGCGGT

Product: adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Products: S-adenosyl-4-methylthio-2-oxobutanoate; 7,8-diaminononanoate

Alternate protein names: 7,8-diamino-pelargonic acid aminotransferase; DAPA aminotransferase [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MDDRERRERHRRLWEADHRHLWHPFTQMRDWLREEPLIVDAAEGVHLVDTLGNRYLDGVSSLWCNVHGHRVPELDAAVRA
QLERVAHSTLLGLASTASIECAEELVRHLPSGLTRVFFSDAGATAVEIAIKMAFQHHQLRGDERRTEFVALHGGYHGDTL
GSVSVGGIDLFHRIFKPLLFDVHHAPQPYCYRCPLGKALPGCGMACADEVERILAAREGRVAALVVEPLVQGADGMITQP
PGYLRRLRELCDRHGALLVCDEVATGWGRTGTTFAVEQEGVLPDILTMAKGLTGGYLPLAATVTTEQVFESFLGEHAEKR
TFFHGHTYAGNPLACAAATASMTLFRERGVGAAVPAKAAALARALAPAARLPHVGEVRQRGLMVGIELVRDRATKEEYAY
ALQAGHRVILEARKLGAILRPLGNVVVLMPPLAMTEAELTRLAGITLAAIEAATARL

Sequences:

>Translated_457_residues
MDDRERRERHRRLWEADHRHLWHPFTQMRDWLREEPLIVDAAEGVHLVDTLGNRYLDGVSSLWCNVHGHRVPELDAAVRA
QLERVAHSTLLGLASTASIECAEELVRHLPSGLTRVFFSDAGATAVEIAIKMAFQHHQLRGDERRTEFVALHGGYHGDTL
GSVSVGGIDLFHRIFKPLLFDVHHAPQPYCYRCPLGKALPGCGMACADEVERILAAREGRVAALVVEPLVQGADGMITQP
PGYLRRLRELCDRHGALLVCDEVATGWGRTGTTFAVEQEGVLPDILTMAKGLTGGYLPLAATVTTEQVFESFLGEHAEKR
TFFHGHTYAGNPLACAAATASMTLFRERGVGAAVPAKAAALARALAPAARLPHVGEVRQRGLMVGIELVRDRATKEEYAY
ALQAGHRVILEARKLGAILRPLGNVVVLMPPLAMTEAELTRLAGITLAAIEAATARL
>Mature_457_residues
MDDRERRERHRRLWEADHRHLWHPFTQMRDWLREEPLIVDAAEGVHLVDTLGNRYLDGVSSLWCNVHGHRVPELDAAVRA
QLERVAHSTLLGLASTASIECAEELVRHLPSGLTRVFFSDAGATAVEIAIKMAFQHHQLRGDERRTEFVALHGGYHGDTL
GSVSVGGIDLFHRIFKPLLFDVHHAPQPYCYRCPLGKALPGCGMACADEVERILAAREGRVAALVVEPLVQGADGMITQP
PGYLRRLRELCDRHGALLVCDEVATGWGRTGTTFAVEQEGVLPDILTMAKGLTGGYLPLAATVTTEQVFESFLGEHAEKR
TFFHGHTYAGNPLACAAATASMTLFRERGVGAAVPAKAAALARALAPAARLPHVGEVRQRGLMVGIELVRDRATKEEYAY
ALQAGHRVILEARKLGAILRPLGNVVVLMPPLAMTEAELTRLAGITLAAIEAATARL

Specific function: Catalyzes the formation of the intermediate 7,8- diaminopelargonic acid (DAPA) from 7-keto-8-aminopelargonic acid (KAPA) with lysine as the amino donor. B.subtilis does not utilize S-adenosylmethionine (SAM), but instead utilizes only L-lysine as the amin

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. BioA subfamily [H]

Homologues:

Organism=Homo sapiens, GI13994255, Length=424, Percent_Identity=29.0094339622642, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI37574042, Length=416, Percent_Identity=27.8846153846154, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI4557809, Length=429, Percent_Identity=29.6037296037296, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI226442705, Length=420, Percent_Identity=27.8571428571429, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI226442709, Length=348, Percent_Identity=29.3103448275862, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI24119277, Length=430, Percent_Identity=27.906976744186, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI284507298, Length=325, Percent_Identity=31.0769230769231, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI188536080, Length=427, Percent_Identity=23.8875878220141, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI38679950, Length=427, Percent_Identity=23.8875878220141, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI38679946, Length=427, Percent_Identity=23.8875878220141, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1786991, Length=439, Percent_Identity=37.3576309794989, Blast_Score=260, Evalue=1e-70,
Organism=Escherichia coli, GI145693181, Length=392, Percent_Identity=35.2040816326531, Blast_Score=194, Evalue=7e-51,
Organism=Escherichia coli, GI1789016, Length=410, Percent_Identity=32.4390243902439, Blast_Score=174, Evalue=7e-45,
Organism=Escherichia coli, GI1788044, Length=408, Percent_Identity=33.8235294117647, Blast_Score=165, Evalue=6e-42,
Organism=Escherichia coli, GI1789759, Length=349, Percent_Identity=34.0974212034384, Blast_Score=158, Evalue=8e-40,
Organism=Escherichia coli, GI1787560, Length=409, Percent_Identity=30.8068459657702, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1786349, Length=365, Percent_Identity=28.7671232876712, Blast_Score=89, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI32564660, Length=444, Percent_Identity=29.0540540540541, Blast_Score=158, Evalue=7e-39,
Organism=Caenorhabditis elegans, GI71992977, Length=435, Percent_Identity=29.6551724137931, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI25144271, Length=426, Percent_Identity=26.2910798122066, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI25144274, Length=240, Percent_Identity=30, Blast_Score=112, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6324386, Length=479, Percent_Identity=29.0187891440501, Blast_Score=202, Evalue=6e-53,
Organism=Saccharomyces cerevisiae, GI6324432, Length=428, Percent_Identity=26.1682242990654, Blast_Score=131, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6323470, Length=450, Percent_Identity=25.3333333333333, Blast_Score=124, Evalue=2e-29,
Organism=Drosophila melanogaster, GI21356575, Length=426, Percent_Identity=30.7511737089202, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI28574759, Length=433, Percent_Identity=30.2540415704388, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI161085790, Length=429, Percent_Identity=30.3030303030303, Blast_Score=142, Evalue=5e-34,
Organism=Drosophila melanogaster, GI21357415, Length=435, Percent_Identity=27.816091954023, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI281366494, Length=402, Percent_Identity=25.1243781094527, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24667139, Length=402, Percent_Identity=25.1243781094527, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24667143, Length=402, Percent_Identity=25.1243781094527, Blast_Score=81, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005814
- InterPro:   IPR005815
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: 2.6.1.62

Molecular weight: Translated: 49927; Mature: 49927

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDRERRERHRRLWEADHRHLWHPFTQMRDWLREEPLIVDAAEGVHLVDTLGNRYLDGVS
CCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHH
SLWCNVHGHRVPELDAAVRAQLERVAHSTLLGLASTASIECAEELVRHLPSGLTRVFFSD
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHCC
AGATAVEIAIKMAFQHHQLRGDERRTEFVALHGGYHGDTLGSVSVGGIDLFHRIFKPLLF
CCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHH
DVHHAPQPYCYRCPLGKALPGCGMACADEVERILAAREGRVAALVVEPLVQGADGMITQP
HHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHCCCCCCCCCC
PGYLRRLRELCDRHGALLVCDEVATGWGRTGTTFAVEQEGVLPDILTMAKGLTGGYLPLA
CHHHHHHHHHHHHCCCEEEECCHHCCCCCCCCEEEEECCCCCHHHHHHHCCCCCCCCHHE
ATVTTEQVFESFLGEHAEKRTFFHGHTYAGNPLACAAATASMTLFRERGVGAAVPAKAAA
EHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHH
LARALAPAARLPHVGEVRQRGLMVGIELVRDRATKEEYAYALQAGHRVILEARKLGAILR
HHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
PLGNVVVLMPPLAMTEAELTRLAGITLAAIEAATARL
CCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDDRERRERHRRLWEADHRHLWHPFTQMRDWLREEPLIVDAAEGVHLVDTLGNRYLDGVS
CCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHH
SLWCNVHGHRVPELDAAVRAQLERVAHSTLLGLASTASIECAEELVRHLPSGLTRVFFSD
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHCC
AGATAVEIAIKMAFQHHQLRGDERRTEFVALHGGYHGDTLGSVSVGGIDLFHRIFKPLLF
CCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHH
DVHHAPQPYCYRCPLGKALPGCGMACADEVERILAAREGRVAALVVEPLVQGADGMITQP
HHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHCCCCCCCCCC
PGYLRRLRELCDRHGALLVCDEVATGWGRTGTTFAVEQEGVLPDILTMAKGLTGGYLPLA
CHHHHHHHHHHHHCCCEEEECCHHCCCCCCCCEEEEECCCCCHHHHHHHCCCCCCCCHHE
ATVTTEQVFESFLGEHAEKRTFFHGHTYAGNPLACAAATASMTLFRERGVGAAVPAKAAA
EHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHH
LARALAPAARLPHVGEVRQRGLMVGIELVRDRATKEEYAYALQAGHRVILEARKLGAILR
HHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
PLGNVVVLMPPLAMTEAELTRLAGITLAAIEAATARL
CCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal Phosphate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; 8-amino-7-oxononanoate

Specific reaction: S-adenosyl-L-methionine + 8-amino-7-oxononanoate = S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate

General reaction: Amino group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8763940; 9387221; 9384377 [H]