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The map label for this gene is tcyB [H]

Identifier: 220914865

GI number: 220914865

Start: 239406

End: 240152

Strand: Reverse

Name: tcyB [H]

Synonym: Mnod_8011

Alternate gene names: 220914865

Gene position: 240152-239406 (Counterclockwise)

Preceding gene: 220914866

Following gene: 220914864

Centisome position: 52.43

GC content: 58.5

Gene sequence:

>747_bases
ATGCTAGAGATCATCGGCCCATTCTTTCGAGGAATGTACGAGCGTACGGGGATGAATTTCAACATATTCTACGATCCGTA
TGAATATGGCCGTTTCGTCTCGGGCGCCTCGACAACCCTGCAGCTGATTTTCTGGTCGTTGTCGCTATCCCTGGTGATCG
GCGTGCTCGGTGCTTGGGCCCAGGGCGCCAAGTCTCCGGCGCTGCGGGTGGGGGTCGGCGCCTACATCCAGGCGTTCCGC
AACACCCCTCCGATGATCCAGCTGCTGTTTTTCTACTTCGGCCTCGGGGCTTTCACCCCTGCTATCGACATGGGCGGCTA
CTCGCAGCCGGTCGTCTCCTCCTTCGCCTGGGCGGTTATCTCCCTGGGCATATTCGGCGGTGCGTTCAACGTCGAGATAT
TCAGGGCAGGCATCGAGGCCGTGCCCGACTCAACTCTCGAGGCGGCCGAAAGTCTCTGCTTTTCGCGTTTGCAAACATAT
CTCTACATCACGCTGCCCTTGGCGTTCCGCATCAGCTTGCCGGCCTTGACCAACAACCTGGTGAGCCTCGCGAAAACGAC
ATCGCTTGCGTACATCATCACGGTTCCGGAGATGACGTACGTCCTGAACCAGGTCTGGTCCGACAACCTGAACGTCCCGG
AGATGATGCTGGTGCTGTTCGGATTTTACATTGTCGTCGTCACGCTCCTGGCGGCCGGCCTGCACGCGATCGAACGCAAG
CTCGTTCTTCCAGGATACGGCCAATGA

Upstream 100 bases:

>100_bases
ACTGGTTCGTGCAGCAGCAACAAAAACTGCATTGGGATACCTCATATCTCAAGTAAATGAGGCCGCTCGAGATTTTCATT
TTGCCTAATGGGGTGTCGAG

Downstream 100 bases:

>100_bases
GACAGCCGGCATTTCAGCTGAACATGGCGCGTCTCCGGGAAGGGCAGTTTCTGAGAGGCCTCCGGGCGGGACACGTCATC
CTCTTGCTCCTGGCCCTGGG

Product: polar amino acid ABC transporter, inner membrane subunit

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MLEIIGPFFRGMYERTGMNFNIFYDPYEYGRFVSGASTTLQLIFWSLSLSLVIGVLGAWAQGAKSPALRVGVGAYIQAFR
NTPPMIQLLFFYFGLGAFTPAIDMGGYSQPVVSSFAWAVISLGIFGGAFNVEIFRAGIEAVPDSTLEAAESLCFSRLQTY
LYITLPLAFRISLPALTNNLVSLAKTTSLAYIITVPEMTYVLNQVWSDNLNVPEMMLVLFGFYIVVVTLLAAGLHAIERK
LVLPGYGQ

Sequences:

>Translated_248_residues
MLEIIGPFFRGMYERTGMNFNIFYDPYEYGRFVSGASTTLQLIFWSLSLSLVIGVLGAWAQGAKSPALRVGVGAYIQAFR
NTPPMIQLLFFYFGLGAFTPAIDMGGYSQPVVSSFAWAVISLGIFGGAFNVEIFRAGIEAVPDSTLEAAESLCFSRLQTY
LYITLPLAFRISLPALTNNLVSLAKTTSLAYIITVPEMTYVLNQVWSDNLNVPEMMLVLFGFYIVVVTLLAAGLHAIERK
LVLPGYGQ
>Mature_248_residues
MLEIIGPFFRGMYERTGMNFNIFYDPYEYGRFVSGASTTLQLIFWSLSLSLVIGVLGAWAQGAKSPALRVGVGAYIQAFR
NTPPMIQLLFFYFGLGAFTPAIDMGGYSQPVVSSFAWAVISLGIFGGAFNVEIFRAGIEAVPDSTLEAAESLCFSRLQTY
LYITLPLAFRISLPALTNNLVSLAKTTSLAYIITVPEMTYVLNQVWSDNLNVPEMMLVLFGFYIVVVTLLAAGLHAIERK
LVLPGYGQ

Specific function: Part of the ABC transporter complex tcyABC involved in L-cystine import. Probably responsible for the translocation of the substrate across the membrane (Probable) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787030, Length=215, Percent_Identity=29.7674418604651, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1788226, Length=210, Percent_Identity=32.3809523809524, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787086, Length=219, Percent_Identity=29.6803652968037, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788645, Length=205, Percent_Identity=26.3414634146341, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788646, Length=170, Percent_Identity=28.2352941176471, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1786874, Length=179, Percent_Identity=26.2569832402235, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 27253; Mature: 27253

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEIIGPFFRGMYERTGMNFNIFYDPYEYGRFVSGASTTLQLIFWSLSLSLVIGVLGAWA
CCCCCCHHHHHHHHHCCCCEEEEECCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QGAKSPALRVGVGAYIQAFRNTPPMIQLLFFYFGLGAFTPAIDMGGYSQPVVSSFAWAVI
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
SLGIFGGAFNVEIFRAGIEAVPDSTLEAAESLCFSRLQTYLYITLPLAFRISLPALTNNL
HHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEEHHHHEEECHHHHHHH
VSLAKTTSLAYIITVPEMTYVLNQVWSDNLNVPEMMLVLFGFYIVVVTLLAAGLHAIERK
HHHHHHCCEEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVLPGYGQ
HCCCCCCC
>Mature Secondary Structure
MLEIIGPFFRGMYERTGMNFNIFYDPYEYGRFVSGASTTLQLIFWSLSLSLVIGVLGAWA
CCCCCCHHHHHHHHHCCCCEEEEECCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QGAKSPALRVGVGAYIQAFRNTPPMIQLLFFYFGLGAFTPAIDMGGYSQPVVSSFAWAVI
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
SLGIFGGAFNVEIFRAGIEAVPDSTLEAAESLCFSRLQTYLYITLPLAFRISLPALTNNL
HHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEEHHHHEEECHHHHHHH
VSLAKTTSLAYIITVPEMTYVLNQVWSDNLNVPEMMLVLFGFYIVVVTLLAAGLHAIERK
HHHHHHCCEEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVLPGYGQ
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7551042; 8969502; 9384377 [H]