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The map label for this gene is stcD [H]

Identifier: 220914861

GI number: 220914861

Start: 234551

End: 236611

Strand: Reverse

Name: stcD [H]

Synonym: Mnod_8007

Alternate gene names: 220914861

Gene position: 236611-234551 (Counterclockwise)

Preceding gene: 220914862

Following gene: 220914860

Centisome position: 51.65

GC content: 67.88

Gene sequence:

>2061_bases
ATGCCGTTAGTCGAAGCGATCACCGGGCCGGCCGCCATCGCGCGCGATCCTCTGCTGCAGCCGCTGCGGATCAAGAACCT
GGTCTTGAGGAACCGCGTCATGAGCACCAGCCACGCCTCCGGCCTGGAGGAAGGCGGAATGCCCAAGGAGCGGTACCAGC
TCTATCATCTGGAGAAGGCGAAGGGCGGCATCGGCCTCAGCATGTTCGGCGGCTCGTCGAACGTGGCGCCCGATAGCCCC
AACATCTTCCGTCAGCTCAATGTCGGCACGGACGAGATCATCCCCTGGCTGCAGCAGTTCTCCGCGCGCATGCATGCTGA
GGGCGCGGCGCTGATGGTGCAGATCACGCATCTCGGTCGCCGCGGCGAGCCGTATGCCGACAAGTGGCTGCCGACGATCG
GACCTTCGGCGATGCGCGAGACGCTGCACCGCAGCTTTCCCAAGGAGATGGACGAGCACGACATCAGGCGCGTCGTGAAG
GCCTACGCGGCCGCCGTGCGCCGCTGCAGGGAAGGCGGGCTCGACGGCGTCGAGACCCTCGCGGGCGGGCACCTCATCGG
CCAGTTCCTCTCCCCCTCCACCAACCGCCGGACGGACAGGTACGGCGGCTCGCTCGAGAACCGCTGCCGCTTCGGCCTCA
TGGTGTTCGAGGAGATACGGAAGGAGGTCGGCGACGACTACATCGTCGGCATGCGCTACGTCATCGAGGAGGGCGAGACC
GGCCTGACGATGGGTGATGCCATCAAGGTGGCGCACATCTTCGAGCGAACCGGCATGCTCGACTTCTTCAACGCCATCGT
CGGCCGCATGGACACCGAGCGGGCGCTCGCCGTGGACAACATGCCCGGCATGGCCTCACCGATCGCGCCCTGGTTGCGGC
AGGTTGGCGCCTTCAAGCGGGAGGTTGGACTTCCGGTGTTCCACGCGGCCCGGATCTCCGACATCGCGACGGCGCGCCAC
GCGATCCGCGAAGGTCTCCTCGACATGGTGGCGATGACCCGCGCCCACATCGCCGACCCCCACATCGTGCGAAAGATCGA
GGCCGGCCAGGAGGACCGCATCCGGCCCTGCGTGGGCGCGACCCACTGCCAGTCGCCCTACCGGCCGCACTGCATCCACA
ACCCCTCCACCGGGCGCGAGAGTCAGCTCCCTCATGAGATCGCCCGATCGGAGCGGGCCGGCCGCAAGGTGGTCGTCGTC
GGAGGCGGGCCCGCCGGCCTTGAGGCGGCCCGGGTCTCGGCCTTGCGCGGGCACGAGGTCGTGCTGTTCGAGGCCGCCGC
CAAGCTCGGCGGGCAGCTGCTGCTGAGCCAGCGTGCGAGCTGGCGGCGCGACGTCATCGGCATCGTGGACTGGCGCGTCG
AGGAGATCCGCCGCTTGGGCGTGCGGGTCGAACTGAACCGCTACGCCGAGGTGGACGATGTGCTCGCCGAGAATCCGGAC
GCGGTCGTGGTTGCCACGGGTGGCGTGCCGGACATCGACTGGATCGCGGGTGCAGAGCACTGCGACAGCGTCTGGGACGT
GATCGGCGGGACCACCCCGCTCGCCGCTGAGGTCATCGTCTATGACGGCACAGGCCGCCACCCCGGCCCGCAGGCGGCCG
AGCTCGCGGCGCAGGATGGCCGCAAAGTCCAGTTCGTTTCGATTGACGCCCAGATCGCGCAGGAATTGACCTATGCCGAG
CGCATCATCTGGAAGAAGCGCATCTACGAGCTCGGCGTGCCGATGACCTTCGACCACGAGATCGTGAAGGTCGAACGGCG
CGGCAACCGCATCGTGGCGACCTTTCGAAACCTGGCCTCCGAACAGCTCACCGAGCGCATGGCCGATCAGATCCTGGTGG
AGCACGGTACGCGACCGGCGGACGACCTGTATCAGGCGCTCAGACCACACTCCGCTAACGACGGTGTCACCGATATCGAC
GCGCTCCTGGCGGGGGAGGCGCAGCCGCTGACCTGGCGTCCCGAGTTGCGCATGGAGCTGCACCGGGTCGGCGACGCCGT
CGCCAGCCGAAACGTGCACGCGGCGGTGCTCGACGCTCTGCGTCTCTGCAAGGATCTCTGA

Upstream 100 bases:

>100_bases
AAGCCCAACTCCTCGCTATCCCGCCTCGCACACGAGACCGGCCGCACGCTGGATCGGCGTGCAAGGCCGACCCGTCCGAC
ATTCCTTCCGGAGAGCTCCC

Downstream 100 bases:

>100_bases
CGGCATGAGAGTGAAGCAGGTTCGGATCTACGTGGTCGAGAGCGGCGGCATCAGGCCGGTCATCCTCGAGATGACGACCG
ACGACGGTACAGTGGGACTG

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: Stachydrine utilization protein stcD [H]

Number of amino acids: Translated: 686; Mature: 685

Protein sequence:

>686_residues
MPLVEAITGPAAIARDPLLQPLRIKNLVLRNRVMSTSHASGLEEGGMPKERYQLYHLEKAKGGIGLSMFGGSSNVAPDSP
NIFRQLNVGTDEIIPWLQQFSARMHAEGAALMVQITHLGRRGEPYADKWLPTIGPSAMRETLHRSFPKEMDEHDIRRVVK
AYAAAVRRCREGGLDGVETLAGGHLIGQFLSPSTNRRTDRYGGSLENRCRFGLMVFEEIRKEVGDDYIVGMRYVIEEGET
GLTMGDAIKVAHIFERTGMLDFFNAIVGRMDTERALAVDNMPGMASPIAPWLRQVGAFKREVGLPVFHAARISDIATARH
AIREGLLDMVAMTRAHIADPHIVRKIEAGQEDRIRPCVGATHCQSPYRPHCIHNPSTGRESQLPHEIARSERAGRKVVVV
GGGPAGLEAARVSALRGHEVVLFEAAAKLGGQLLLSQRASWRRDVIGIVDWRVEEIRRLGVRVELNRYAEVDDVLAENPD
AVVVATGGVPDIDWIAGAEHCDSVWDVIGGTTPLAAEVIVYDGTGRHPGPQAAELAAQDGRKVQFVSIDAQIAQELTYAE
RIIWKKRIYELGVPMTFDHEIVKVERRGNRIVATFRNLASEQLTERMADQILVEHGTRPADDLYQALRPHSANDGVTDID
ALLAGEAQPLTWRPELRMELHRVGDAVASRNVHAAVLDALRLCKDL

Sequences:

>Translated_686_residues
MPLVEAITGPAAIARDPLLQPLRIKNLVLRNRVMSTSHASGLEEGGMPKERYQLYHLEKAKGGIGLSMFGGSSNVAPDSP
NIFRQLNVGTDEIIPWLQQFSARMHAEGAALMVQITHLGRRGEPYADKWLPTIGPSAMRETLHRSFPKEMDEHDIRRVVK
AYAAAVRRCREGGLDGVETLAGGHLIGQFLSPSTNRRTDRYGGSLENRCRFGLMVFEEIRKEVGDDYIVGMRYVIEEGET
GLTMGDAIKVAHIFERTGMLDFFNAIVGRMDTERALAVDNMPGMASPIAPWLRQVGAFKREVGLPVFHAARISDIATARH
AIREGLLDMVAMTRAHIADPHIVRKIEAGQEDRIRPCVGATHCQSPYRPHCIHNPSTGRESQLPHEIARSERAGRKVVVV
GGGPAGLEAARVSALRGHEVVLFEAAAKLGGQLLLSQRASWRRDVIGIVDWRVEEIRRLGVRVELNRYAEVDDVLAENPD
AVVVATGGVPDIDWIAGAEHCDSVWDVIGGTTPLAAEVIVYDGTGRHPGPQAAELAAQDGRKVQFVSIDAQIAQELTYAE
RIIWKKRIYELGVPMTFDHEIVKVERRGNRIVATFRNLASEQLTERMADQILVEHGTRPADDLYQALRPHSANDGVTDID
ALLAGEAQPLTWRPELRMELHRVGDAVASRNVHAAVLDALRLCKDL
>Mature_685_residues
PLVEAITGPAAIARDPLLQPLRIKNLVLRNRVMSTSHASGLEEGGMPKERYQLYHLEKAKGGIGLSMFGGSSNVAPDSPN
IFRQLNVGTDEIIPWLQQFSARMHAEGAALMVQITHLGRRGEPYADKWLPTIGPSAMRETLHRSFPKEMDEHDIRRVVKA
YAAAVRRCREGGLDGVETLAGGHLIGQFLSPSTNRRTDRYGGSLENRCRFGLMVFEEIRKEVGDDYIVGMRYVIEEGETG
LTMGDAIKVAHIFERTGMLDFFNAIVGRMDTERALAVDNMPGMASPIAPWLRQVGAFKREVGLPVFHAARISDIATARHA
IREGLLDMVAMTRAHIADPHIVRKIEAGQEDRIRPCVGATHCQSPYRPHCIHNPSTGRESQLPHEIARSERAGRKVVVVG
GGPAGLEAARVSALRGHEVVLFEAAAKLGGQLLLSQRASWRRDVIGIVDWRVEEIRRLGVRVELNRYAEVDDVLAENPDA
VVVATGGVPDIDWIAGAEHCDSVWDVIGGTTPLAAEVIVYDGTGRHPGPQAAELAAQDGRKVQFVSIDAQIAQELTYAER
IIWKKRIYELGVPMTFDHEIVKVERRGNRIVATFRNLASEQLTERMADQILVEHGTRPADDLYQALRPHSANDGVTDIDA
LLAGEAQPLTWRPELRMELHRVGDAVASRNVHAAVLDALRLCKDL

Specific function: Possible NADH-dependent oxidase, functions as a demethylase that converts N-methylproline to proline [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789463, Length=521, Percent_Identity=29.5585412667946, Blast_Score=208, Evalue=8e-55,
Organism=Escherichia coli, GI1787939, Length=363, Percent_Identity=25.8953168044077, Blast_Score=86, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17559804, Length=313, Percent_Identity=27.1565495207668, Blast_Score=89, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17559802, Length=270, Percent_Identity=29.6296296296296, Blast_Score=89, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17564188, Length=171, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17540738, Length=179, Percent_Identity=31.8435754189944, Blast_Score=82, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17565138, Length=255, Percent_Identity=29.0196078431373, Blast_Score=82, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI72001454, Length=169, Percent_Identity=33.7278106508876, Blast_Score=80, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17566914, Length=360, Percent_Identity=24.7222222222222, Blast_Score=72, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6321973, Length=257, Percent_Identity=28.4046692607004, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6325086, Length=253, Percent_Identity=26.4822134387352, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 75689; Mature: 75557

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLVEAITGPAAIARDPLLQPLRIKNLVLRNRVMSTSHASGLEEGGMPKERYQLYHLEKA
CCCHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHC
KGGIGLSMFGGSSNVAPDSPNIFRQLNVGTDEIIPWLQQFSARMHAEGAALMVQITHLGR
CCCCEEEEECCCCCCCCCCCHHHHEECCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
RGEPYADKWLPTIGPSAMRETLHRSFPKEMDEHDIRRVVKAYAAAVRRCREGGLDGVETL
CCCCCHHHCCCCCCHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
AGGHLIGQFLSPSTNRRTDRYGGSLENRCRFGLMVFEEIRKEVGDDYIVGMRYVIEEGET
HCHHHHHHHHCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHEECCCC
GLTMGDAIKVAHIFERTGMLDFFNAIVGRMDTERALAVDNMPGMASPIAPWLRQVGAFKR
CCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHEEECCCCCCCCHHHHHHHHHHHHHH
EVGLPVFHAARISDIATARHAIREGLLDMVAMTRAHIADPHIVRKIEAGQEDRIRPCVGA
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCC
THCQSPYRPHCIHNPSTGRESQLPHEIARSERAGRKVVVVGGGPAGLEAARVSALRGHEV
HHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCCEE
VLFEAAAKLGGQLLLSQRASWRRDVIGIVDWRVEEIRRLGVRVELNRYAEVDDVLAENPD
EEEHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHCCCC
AVVVATGGVPDIDWIAGAEHCDSVWDVIGGTTPLAAEVIVYDGTGRHPGPQAAELAAQDG
EEEEECCCCCCCHHHCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHCCC
RKVQFVSIDAQIAQELTYAERIIWKKRIYELGVPMTFDHEIVKVERRGNRIVATFRNLAS
CEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEECCHHHEEECCCCEEHHHHHHHHH
EQLTERMADQILVEHGTRPADDLYQALRPHSANDGVTDIDALLAGEAQPLTWRPELRMEL
HHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCEECHHHHHHH
HRVGDAVASRNVHAAVLDALRLCKDL
HHHHHHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PLVEAITGPAAIARDPLLQPLRIKNLVLRNRVMSTSHASGLEEGGMPKERYQLYHLEKA
CCHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHC
KGGIGLSMFGGSSNVAPDSPNIFRQLNVGTDEIIPWLQQFSARMHAEGAALMVQITHLGR
CCCCEEEEECCCCCCCCCCCHHHHEECCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
RGEPYADKWLPTIGPSAMRETLHRSFPKEMDEHDIRRVVKAYAAAVRRCREGGLDGVETL
CCCCCHHHCCCCCCHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
AGGHLIGQFLSPSTNRRTDRYGGSLENRCRFGLMVFEEIRKEVGDDYIVGMRYVIEEGET
HCHHHHHHHHCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHEECCCC
GLTMGDAIKVAHIFERTGMLDFFNAIVGRMDTERALAVDNMPGMASPIAPWLRQVGAFKR
CCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHEEECCCCCCCCHHHHHHHHHHHHHH
EVGLPVFHAARISDIATARHAIREGLLDMVAMTRAHIADPHIVRKIEAGQEDRIRPCVGA
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCC
THCQSPYRPHCIHNPSTGRESQLPHEIARSERAGRKVVVVGGGPAGLEAARVSALRGHEV
HHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCCEE
VLFEAAAKLGGQLLLSQRASWRRDVIGIVDWRVEEIRRLGVRVELNRYAEVDDVLAENPD
EEEHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHCCCC
AVVVATGGVPDIDWIAGAEHCDSVWDVIGGTTPLAAEVIVYDGTGRHPGPQAAELAAQDG
EEEEECCCCCCCHHHCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHCCC
RKVQFVSIDAQIAQELTYAERIIWKKRIYELGVPMTFDHEIVKVERRGNRIVATFRNLAS
CEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEECCHHHEEECCCCEEHHHHHHHHH
EQLTERMADQILVEHGTRPADDLYQALRPHSANDGVTDIDALLAGEAQPLTWRPELRMEL
HHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCEECHHHHHHH
HRVGDAVASRNVHAAVLDALRLCKDL
HHHHHHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9758825; 11481431 [H]