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The map label for this gene is braE [H]

Identifier: 220914813

GI number: 220914813

Start: 174160

End: 175041

Strand: Reverse

Name: braE [H]

Synonym: Mnod_7950

Alternate gene names: 220914813

Gene position: 175041-174160 (Counterclockwise)

Preceding gene: 220914814

Following gene: 220914812

Centisome position: 38.21

GC content: 69.73

Gene sequence:

>882_bases
ATGGATTATCTCTTCACCCTCGCCATCCTGATCGGGCTGAGCATCGCGCTCGCGTCGAGCTTCAACCTGATCATCGGCTA
TGCCGGACTGATCTCCATCGCTCACCCGGTCTTCTACGCGATCGGCGCCTACGTCTCGGCGCTGCTCGCCCGCGACCTCG
GCGTTCCGGTTCCGCTCGCGATGCTCTGCGGTGCGCTCGCTGCGGCGGCAGCCTCGGCGGCCGTCGCCCTGCCGTCCCTG
CGCGTCTCGGGCGACTACCTGCTGATCGCCAGCATCGGCTTCCAGCTCGGCGTCCTCGAGGCGATCAAGAACATCGCCGT
CACCGGCGGGGCCGGCGGCCTGACCAACATCCCGCCGTTCCTCGTCCACGAGGCGGGCCGCGGCGCCTACGTGGCGCTGG
TGCTCGGCTTCGCCGCCCTGACGGTGTGGCTGAACTGGACCATCGCGCACGGCCCCTACGGCCGGGCCTTGAGCGCAATG
CGTGACGATGAACTCGCCTTCGCGGGGCTGGGACGCAATGCAGTGTCCATGAAGGTCGCCGTGTTCGCCTTCGGATCAGC
CGTCGCGGGCCTCGCGGGCGCCCTCTACGCCCACTACTTCCGCTTCGTCACCCCGGAGCAGTTCGAGATCCTGCAATCGG
CTGCCCTGCTCACCATGGTCGTCGTCGGGGGCATCCGCACCACCTGGGGGCCGGTCCTCGGCGCGGTGCTGCTCCAGGCG
CTGCCCCAGGCGATCACCTTCCTCGACCTGCCGCCCGCGCTGCTCGGCCCGCTCCAGGGCCTCATGTTCACCGGCCTCGT
CCTCGTCTTCATGTTCTGGCGGCCGCAAGGGCTGATCGCCGCCGGCGACGCGTGGCGCCCCTCGAAGCGGGAGACCGCCT
GA

Upstream 100 bases:

>100_bases
ACCGGCGGCGCGGCCTCGCGCGCCGGATCGCGCGCGTCGATCTCGGCGAAACGAAGCCGGCCGAGACCGCCGCGGCCTCG
GGTCGGGGAAAGGCTTGATC

Downstream 100 bases:

>100_bases
TGACGCCGGTCCTTGAGATCGCCGGCCTCGACAAGCGCTTCGGCGGGATCGTCGTGGCGGACGGCATCGACCTGACGCTC
CCGGCCGGGCGCATCGTCGG

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MDYLFTLAILIGLSIALASSFNLIIGYAGLISIAHPVFYAIGAYVSALLARDLGVPVPLAMLCGALAAAAASAAVALPSL
RVSGDYLLIASIGFQLGVLEAIKNIAVTGGAGGLTNIPPFLVHEAGRGAYVALVLGFAALTVWLNWTIAHGPYGRALSAM
RDDELAFAGLGRNAVSMKVAVFAFGSAVAGLAGALYAHYFRFVTPEQFEILQSAALLTMVVVGGIRTTWGPVLGAVLLQA
LPQAITFLDLPPALLGPLQGLMFTGLVLVFMFWRPQGLIAAGDAWRPSKRETA

Sequences:

>Translated_293_residues
MDYLFTLAILIGLSIALASSFNLIIGYAGLISIAHPVFYAIGAYVSALLARDLGVPVPLAMLCGALAAAAASAAVALPSL
RVSGDYLLIASIGFQLGVLEAIKNIAVTGGAGGLTNIPPFLVHEAGRGAYVALVLGFAALTVWLNWTIAHGPYGRALSAM
RDDELAFAGLGRNAVSMKVAVFAFGSAVAGLAGALYAHYFRFVTPEQFEILQSAALLTMVVVGGIRTTWGPVLGAVLLQA
LPQAITFLDLPPALLGPLQGLMFTGLVLVFMFWRPQGLIAAGDAWRPSKRETA
>Mature_293_residues
MDYLFTLAILIGLSIALASSFNLIIGYAGLISIAHPVFYAIGAYVSALLARDLGVPVPLAMLCGALAAAAASAAVALPSL
RVSGDYLLIASIGFQLGVLEAIKNIAVTGGAGGLTNIPPFLVHEAGRGAYVALVLGFAALTVWLNWTIAHGPYGRALSAM
RDDELAFAGLGRNAVSMKVAVFAFGSAVAGLAGALYAHYFRFVTPEQFEILQSAALLTMVVVGGIRTTWGPVLGAVLLQA
LPQAITFLDLPPALLGPLQGLMFTGLVLVFMFWRPQGLIAAGDAWRPSKRETA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=301, Percent_Identity=31.2292358803987, Blast_Score=115, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 30454; Mature: 30454

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYLFTLAILIGLSIALASSFNLIIGYAGLISIAHPVFYAIGAYVSALLARDLGVPVPLA
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
MLCGALAAAAASAAVALPSLRVSGDYLLIASIGFQLGVLEAIKNIAVTGGAGGLTNIPPF
HHHHHHHHHHHHHHHHCCCEEECCCEEEEEHHHHHHHHHHHHHHHEEECCCCCCCCCCHH
LVHEAGRGAYVALVLGFAALTVWLNWTIAHGPYGRALSAMRDDELAFAGLGRNAVSMKVA
HHCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHCCCCHHHHCCCCCHHHHHHH
VFAFGSAVAGLAGALYAHYFRFVTPEQFEILQSAALLTMVVVGGIRTTWGPVLGAVLLQA
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LPQAITFLDLPPALLGPLQGLMFTGLVLVFMFWRPQGLIAAGDAWRPSKRETA
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCC
>Mature Secondary Structure
MDYLFTLAILIGLSIALASSFNLIIGYAGLISIAHPVFYAIGAYVSALLARDLGVPVPLA
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
MLCGALAAAAASAAVALPSLRVSGDYLLIASIGFQLGVLEAIKNIAVTGGAGGLTNIPPF
HHHHHHHHHHHHHHHHCCCEEECCCEEEEEHHHHHHHHHHHHHHHEEECCCCCCCCCCHH
LVHEAGRGAYVALVLGFAALTVWLNWTIAHGPYGRALSAMRDDELAFAGLGRNAVSMKVA
HHCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHCCCCHHHHCCCCCHHHHHHH
VFAFGSAVAGLAGALYAHYFRFVTPEQFEILQSAALLTMVVVGGIRTTWGPVLGAVLLQA
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LPQAITFLDLPPALLGPLQGLMFTGLVLVFMFWRPQGLIAAGDAWRPSKRETA
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]