Click here to switch to the map view.

The map label for this gene is rffG [C]

Identifier: 220914794

GI number: 220914794

Start: 155406

End: 156374

Strand: Reverse

Name: rffG [C]

Synonym: Mnod_7928

Alternate gene names: 220914794

Gene position: 156374-155406 (Counterclockwise)

Preceding gene: 220914795

Following gene: 220914793

Centisome position: 34.14

GC content: 69.35

Gene sequence:

>969_bases
GTGACCACCCTCATCACCGGCGTCTCCGGCTTCGTGGGCGGCGCGCTTGCCGCCGCTCTCGTCGCCCGGAGCGAGGACGT
GATCGGCGTGGATCGTGCCCCATCCCCGGCCGATCCTCGGGAAGGACACGGCACGTACCGGTTCCAGGCTGCCGACCTGC
GCCAGAATGGAGCGCTCGACCCCATCCTCGCCGAAGCCCGTGTAGACCGGCTCATCATCGGCGCAGCGATCACGGCCGAT
GCCGCCCGCGAGCGCAGCGATCCGGTGGGCGTCATCACGGTCAATGTCGCCGCCGTAGCTGACGCCGTCCGCTCGGCCGC
ACGCCACAATGTGGGGCGTGTGCTTTATCTCGGCTCCGGCGCAGTCTACGGCGACAGCGCAGCCGGCGCGGGAGCGCTGG
TTGAGGACGAGACGCCGCTCAGGCCGCGCTCGCTGTATGCAATCACCAAGCAGGCCGGCGAGGCCACGGCCCTGCGCCTC
GCCGACACCTTCGGCCTGGATCTCGTCGCAGCCCGGCTCGGCACCTGCTTCGGCCCCTATGAACGCGACACGGGCATGCG
CGATACCTTGAGCGCGCCCTACCAGATCCTGCGCCTTGCCGCGTCAGGCGAGCCGGTCCGGCTGCCACGCCCGGGCCGGC
GCGACTGGCTCTACATTCGCGATGCGGTCTCGGGCCTGCTCGATGCCGAGCGGCTGAACCATCCCGTCTACAACGTGGCG
GCGGGCCACGAATGGCCGCTGACGGAATGGTGCGCGCATGTCGCGAGCGCCTATTCAGACTTCCGCTGGTCGGTCTCGGG
GCCGGAGACGGCCAACGTCAACCTCTACGATGCCTTCGACCGGGCGCCGATGAGCGTTGCCCGCATCACGGCCGACACCA
CCTACCGGCCAGCCTTCGACCTCGCGGCAGTCGCACGAGATTTCCTCCAAGAGCCTTTCCTCCAAGAGCCCTGGAGCGCA
CGGCAATGA

Upstream 100 bases:

>100_bases
GGCCGAAGTGTCCAAGAACATGGACTACAGCTTCCTCCAGGCCGCGTCCGGTCTGTCAGCGGCCGATCTCGGCAACAAGT
AGCATCGGGGACGAGGCGCC

Downstream 100 bases:

>100_bases
CGGACGCAGAGCCGATCGCCGTCGACGGCAATCGCCGCCTCGCCGGCCAGACCTGCATCGTTACCGGGGCGGCCTCCGGG
ATCGGGCGAGCCATCGCGCG

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MTTLITGVSGFVGGALAAALVARSEDVIGVDRAPSPADPREGHGTYRFQAADLRQNGALDPILAEARVDRLIIGAAITAD
AARERSDPVGVITVNVAAVADAVRSAARHNVGRVLYLGSGAVYGDSAAGAGALVEDETPLRPRSLYAITKQAGEATALRL
ADTFGLDLVAARLGTCFGPYERDTGMRDTLSAPYQILRLAASGEPVRLPRPGRRDWLYIRDAVSGLLDAERLNHPVYNVA
AGHEWPLTEWCAHVASAYSDFRWSVSGPETANVNLYDAFDRAPMSVARITADTTYRPAFDLAAVARDFLQEPFLQEPWSA
RQ

Sequences:

>Translated_322_residues
MTTLITGVSGFVGGALAAALVARSEDVIGVDRAPSPADPREGHGTYRFQAADLRQNGALDPILAEARVDRLIIGAAITAD
AARERSDPVGVITVNVAAVADAVRSAARHNVGRVLYLGSGAVYGDSAAGAGALVEDETPLRPRSLYAITKQAGEATALRL
ADTFGLDLVAARLGTCFGPYERDTGMRDTLSAPYQILRLAASGEPVRLPRPGRRDWLYIRDAVSGLLDAERLNHPVYNVA
AGHEWPLTEWCAHVASAYSDFRWSVSGPETANVNLYDAFDRAPMSVARITADTTYRPAFDLAAVARDFLQEPFLQEPWSA
RQ
>Mature_321_residues
TTLITGVSGFVGGALAAALVARSEDVIGVDRAPSPADPREGHGTYRFQAADLRQNGALDPILAEARVDRLIIGAAITADA
ARERSDPVGVITVNVAAVADAVRSAARHNVGRVLYLGSGAVYGDSAAGAGALVEDETPLRPRSLYAITKQAGEATALRLA
DTFGLDLVAARLGTCFGPYERDTGMRDTLSAPYQILRLAASGEPVRLPRPGRRDWLYIRDAVSGLLDAERLNHPVYNVAA
GHEWPLTEWCAHVASAYSDFRWSVSGPETANVNLYDAFDRAPMSVARITADTTYRPAFDLAAVARDFLQEPFLQEPWSAR
Q

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 34474; Mature: 34343

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLITGVSGFVGGALAAALVARSEDVIGVDRAPSPADPREGHGTYRFQAADLRQNGALD
CCEEECCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCC
PILAEARVDRLIIGAAITADAARERSDPVGVITVNVAAVADAVRSAARHNVGRVLYLGSG
HHHHHHHHHHEEEEHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCCCEEEEEECC
AVYGDSAAGAGALVEDETPLRPRSLYAITKQAGEATALRLADTFGLDLVAARLGTCFGPY
CEECCCCCCCCCEECCCCCCCCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHCCCC
ERDTGMRDTLSAPYQILRLAASGEPVRLPRPGRRDWLYIRDAVSGLLDAERLNHPVYNVA
CCCCCCHHHHHHHHHHHHHHCCCCCEECCCCCCCCEEEHHHHHHHHHHHHHHCCCEEEEC
AGHEWPLTEWCAHVASAYSDFRWSVSGPETANVNLYDAFDRAPMSVARITADTTYRPAFD
CCCCCCHHHHHHHHHHHHHHCEEECCCCCCCCEEEEECCCCCCCEEEEEECCCCCCCHHH
LAAVARDFLQEPFLQEPWSARQ
HHHHHHHHHHCCHHCCCCCCCC
>Mature Secondary Structure 
TTLITGVSGFVGGALAAALVARSEDVIGVDRAPSPADPREGHGTYRFQAADLRQNGALD
CEEECCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCC
PILAEARVDRLIIGAAITADAARERSDPVGVITVNVAAVADAVRSAARHNVGRVLYLGSG
HHHHHHHHHHEEEEHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCCCEEEEEECC
AVYGDSAAGAGALVEDETPLRPRSLYAITKQAGEATALRLADTFGLDLVAARLGTCFGPY
CEECCCCCCCCCEECCCCCCCCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHCCCC
ERDTGMRDTLSAPYQILRLAASGEPVRLPRPGRRDWLYIRDAVSGLLDAERLNHPVYNVA
CCCCCCHHHHHHHHHHHHHHCCCCCEECCCCCCCCEEEHHHHHHHHHHHHHHCCCEEEEC
AGHEWPLTEWCAHVASAYSDFRWSVSGPETANVNLYDAFDRAPMSVARITADTTYRPAFD
CCCCCCHHHHHHHHHHHHHHCEEECCCCCCCCEEEEECCCCCCCEEEEEECCCCCCCHHH
LAAVARDFLQEPFLQEPWSARQ
HHHHHHHHHHCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]