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The map label for this gene is flbD [H]

Identifier: 220905641

GI number: 220905641

Start: 2870814

End: 2872331

Strand: Reverse

Name: flbD [H]

Synonym: Ddes_2381

Alternate gene names: 220905641

Gene position: 2872331-2870814 (Counterclockwise)

Preceding gene: NA

Following gene: 220905633

Centisome position: 99.96

GC content: 59.75

Gene sequence:

>1518_bases
ATGTTGACGCGCCGTCTGCTATTTCTCACCCCCGCTCAGGCCGTGACCTCGGTCTTTCCTGCCCTGCGTGATGCCGGATA
TGAACTTGGCATTGCGGAAAATCTCAAGGGCGCCTCCGTCTTCATTCGCAAGTCCGGACCGGGCATCATTTTTGCGCGGC
CCAGCCTGCCTGGTTTTCGCGTTGAGGATCTTCTGGCTGTAGGGGCTGAAGACCCTGCTTTTCCCCCGGTTATTGTCATT
ACGGACAGGGGAACCGCCGATGAGGCCGAACGCCTCATGAGTCTCGGAGCACAGGATTACTGGCTTGAACCGCTGGATGT
AGAGCGCATCATGGCCGTGGCGCGGTCGCTGGGCAACGGGGCCGGGCAGCGCAAGGCGGCTGCGCCTGCCACCTCTACCC
TTGGAGGGCGCAAGGCCCATTATGGAGAGGGCGCCGGGCCGCGCATTGTGGGCAAACATCCGGCCATGGGCAGAGTTTTG
CATCTGGCGCGTCAGGTGGCCGGTTCGCGGGCAACGGTACTGATTACCGGCGAATCAGGCACGGGCAAGGAAATGTTTGC
CCGGTATCTGCACGCCATGAGCAAACGTGGTGACGGGGCCTTTGTGGCCGTCAACTGCGCCGCCCTGCCGGAGCACCTTC
TTGAAAGCGAACTTTTCGGGCATGAAAAAGGCGCGTTTACCGGCGCCATTGCCCGCAAGCCGGGTAAATTCGAGCTTGCT
GACGGCGGCACACTGCTGCTGGACGAAATTTCAGAAATGGACCTTTCCCTTCAGGCCAAGCTTTTGCGCGTCCTGCAGGA
GGGTGAGGTGGACCGAGTGGGCGGCACAGAACCGCTCAAGGTTAATGTGCGTGTGCTGGCCACCACCAACCGGAACCTTG
AAGACTGGGTAAAGCAGGGAAAATTTCGTCAGGACCTGTATTTCAGGCTTAATGTTATTCCTTTGCGCCTGCCTTCATTG
CGGGAACGTGGCGATGATGTTCTGGAGCTGGCCGGATTTTTTATGGACATGTACATACGCGAATACCAGCTTGCTTCGAC
AGTGCTTTCGCAAAGCGCCATAGACTGGCTGCGCGAGTATGATTTTCCCGGCAACGTGCGTGAACTGCAGAATCTGATGG
AACGCGCTGTCCTGCTTGCCAATGGCCGACCTGTAGAGCCGCGCCATTTTCTGCTGGAAGATGACGACTGGCCGCTGTTT
GAGGATGACAGCGCGGACTTTGCCCCCGAACATTCCGGGGCCGCGGCTGTGCCGGACTTTTCCGGTGGCGTGGCGCTTGC
CGCAGACACGTCGCCGCGAAGTGCCGCCGCTGGTGGTGGGCCTGCGGTGTTGCCTTCAGCCGGTGGTATGCCCGGTGCGG
TTATCCCCCTGCATGAGATGGAGCGGATCATGATCCTCAAGGGGCTTGAAGCAACATCCGGAAATCGCACACAGGCCGCC
GACCTTCTTGGCATTTCTGTGCGGACCCTGCGCAACAAGCTCAACGAATACCGCGCTGCCGGCCACCCTGTAGACTGA

Upstream 100 bases:

>100_bases
ATGGGCCAACTGGTCGTTTTATGGCGTAAAAAGCTCCTGTTCCAGTGTATTTCAACATCCGTTGGTTGCATTGTATCGCC
TGACACGGTAAGAAAATTTC

Downstream 100 bases:

>100_bases
TTGGCGTCTGAGGCATCGCCTTGCCTGTGGAGAGCGCTTTGGGCTTGAGGATACAGATTCTCAAGGTTTCAGGGCGTTCA
TTTCGGCGGTGACCCGCGCA

Product: Fis family two component sigma-54 specific transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MLTRRLLFLTPAQAVTSVFPALRDAGYELGIAENLKGASVFIRKSGPGIIFARPSLPGFRVEDLLAVGAEDPAFPPVIVI
TDRGTADEAERLMSLGAQDYWLEPLDVERIMAVARSLGNGAGQRKAAAPATSTLGGRKAHYGEGAGPRIVGKHPAMGRVL
HLARQVAGSRATVLITGESGTGKEMFARYLHAMSKRGDGAFVAVNCAALPEHLLESELFGHEKGAFTGAIARKPGKFELA
DGGTLLLDEISEMDLSLQAKLLRVLQEGEVDRVGGTEPLKVNVRVLATTNRNLEDWVKQGKFRQDLYFRLNVIPLRLPSL
RERGDDVLELAGFFMDMYIREYQLASTVLSQSAIDWLREYDFPGNVRELQNLMERAVLLANGRPVEPRHFLLEDDDWPLF
EDDSADFAPEHSGAAAVPDFSGGVALAADTSPRSAAAGGGPAVLPSAGGMPGAVIPLHEMERIMILKGLEATSGNRTQAA
DLLGISVRTLRNKLNEYRAAGHPVD

Sequences:

>Translated_505_residues
MLTRRLLFLTPAQAVTSVFPALRDAGYELGIAENLKGASVFIRKSGPGIIFARPSLPGFRVEDLLAVGAEDPAFPPVIVI
TDRGTADEAERLMSLGAQDYWLEPLDVERIMAVARSLGNGAGQRKAAAPATSTLGGRKAHYGEGAGPRIVGKHPAMGRVL
HLARQVAGSRATVLITGESGTGKEMFARYLHAMSKRGDGAFVAVNCAALPEHLLESELFGHEKGAFTGAIARKPGKFELA
DGGTLLLDEISEMDLSLQAKLLRVLQEGEVDRVGGTEPLKVNVRVLATTNRNLEDWVKQGKFRQDLYFRLNVIPLRLPSL
RERGDDVLELAGFFMDMYIREYQLASTVLSQSAIDWLREYDFPGNVRELQNLMERAVLLANGRPVEPRHFLLEDDDWPLF
EDDSADFAPEHSGAAAVPDFSGGVALAADTSPRSAAAGGGPAVLPSAGGMPGAVIPLHEMERIMILKGLEATSGNRTQAA
DLLGISVRTLRNKLNEYRAAGHPVD
>Mature_505_residues
MLTRRLLFLTPAQAVTSVFPALRDAGYELGIAENLKGASVFIRKSGPGIIFARPSLPGFRVEDLLAVGAEDPAFPPVIVI
TDRGTADEAERLMSLGAQDYWLEPLDVERIMAVARSLGNGAGQRKAAAPATSTLGGRKAHYGEGAGPRIVGKHPAMGRVL
HLARQVAGSRATVLITGESGTGKEMFARYLHAMSKRGDGAFVAVNCAALPEHLLESELFGHEKGAFTGAIARKPGKFELA
DGGTLLLDEISEMDLSLQAKLLRVLQEGEVDRVGGTEPLKVNVRVLATTNRNLEDWVKQGKFRQDLYFRLNVIPLRLPSL
RERGDDVLELAGFFMDMYIREYQLASTVLSQSAIDWLREYDFPGNVRELQNLMERAVLLANGRPVEPRHFLLEDDDWPLF
EDDSADFAPEHSGAAAVPDFSGGVALAADTSPRSAAAGGGPAVLPSAGGMPGAVIPLHEMERIMILKGLEATSGNRTQAA
DLLGISVRTLRNKLNEYRAAGHPVD

Specific function: Activation of sigma-54-dependent flagellar gene promoters and strong negative autoregulatory effects on its own promoter. The synthesis and function of flbD in C.crescentus is controlled by an internal cell-cycle clock [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=475, Percent_Identity=37.2631578947368, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI1788550, Length=477, Percent_Identity=37.5262054507338, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI1790299, Length=480, Percent_Identity=38.125, Blast_Score=261, Evalue=9e-71,
Organism=Escherichia coli, GI87082152, Length=257, Percent_Identity=50.9727626459144, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI87082117, Length=257, Percent_Identity=50.1945525291829, Blast_Score=244, Evalue=8e-66,
Organism=Escherichia coli, GI1789233, Length=352, Percent_Identity=38.6363636363636, Blast_Score=228, Evalue=6e-61,
Organism=Escherichia coli, GI1789087, Length=256, Percent_Identity=47.265625, Blast_Score=226, Evalue=4e-60,
Organism=Escherichia coli, GI1788905, Length=307, Percent_Identity=41.042345276873, Blast_Score=224, Evalue=7e-60,
Organism=Escherichia coli, GI87081872, Length=230, Percent_Identity=47.8260869565217, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1786524, Length=252, Percent_Identity=46.4285714285714, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1787583, Length=253, Percent_Identity=41.897233201581, Blast_Score=187, Evalue=9e-49,
Organism=Escherichia coli, GI1789828, Length=183, Percent_Identity=39.344262295082, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI87081858, Length=236, Percent_Identity=30.9322033898305, Blast_Score=111, Evalue=9e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 54473; Mature: 54473

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRRLLFLTPAQAVTSVFPALRDAGYELGIAENLKGASVFIRKSGPGIIFARPSLPGFR
CCCCEEEEECCHHHHHHHHHHHHHCCCCCCHHCCCCCCEEEEEECCCEEEEECCCCCCCC
VEDLLAVGAEDPAFPPVIVITDRGTADEAERLMSLGAQDYWLEPLDVERIMAVARSLGNG
HHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCC
AGQRKAAAPATSTLGGRKAHYGEGAGPRIVGKHPAMGRVLHLARQVAGSRATVLITGESG
CCCCCCCCCCHHCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHCCCCEEEEEECCCC
TGKEMFARYLHAMSKRGDGAFVAVNCAALPEHLLESELFGHEKGAFTGAIARKPGKFELA
CCHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCEEHHHHCCCCCEEEC
DGGTLLLDEISEMDLSLQAKLLRVLQEGEVDRVGGTEPLKVNVRVLATTNRNLEDWVKQG
CCCEEEEHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCHHHHHHCC
KFRQDLYFRLNVIPLRLPSLRERGDDVLELAGFFMDMYIREYQLASTVLSQSAIDWLREY
CCCCCEEEEEEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DFPGNVRELQNLMERAVLLANGRPVEPRHFLLEDDDWPLFEDDSADFAPEHSGAAAVPDF
CCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
SGGVALAADTSPRSAAAGGGPAVLPSAGGMPGAVIPLHEMERIMILKGLEATSGNRTQAA
CCCEEEEECCCCCCCCCCCCCEECCCCCCCCCCEECHHHHHHHHHEECCCCCCCCCCHHH
DLLGISVRTLRNKLNEYRAAGHPVD
HHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLTRRLLFLTPAQAVTSVFPALRDAGYELGIAENLKGASVFIRKSGPGIIFARPSLPGFR
CCCCEEEEECCHHHHHHHHHHHHHCCCCCCHHCCCCCCEEEEEECCCEEEEECCCCCCCC
VEDLLAVGAEDPAFPPVIVITDRGTADEAERLMSLGAQDYWLEPLDVERIMAVARSLGNG
HHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCC
AGQRKAAAPATSTLGGRKAHYGEGAGPRIVGKHPAMGRVLHLARQVAGSRATVLITGESG
CCCCCCCCCCHHCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHCCCCEEEEEECCCC
TGKEMFARYLHAMSKRGDGAFVAVNCAALPEHLLESELFGHEKGAFTGAIARKPGKFELA
CCHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCEEHHHHCCCCCEEEC
DGGTLLLDEISEMDLSLQAKLLRVLQEGEVDRVGGTEPLKVNVRVLATTNRNLEDWVKQG
CCCEEEEHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCHHHHHHCC
KFRQDLYFRLNVIPLRLPSLRERGDDVLELAGFFMDMYIREYQLASTVLSQSAIDWLREY
CCCCCEEEEEEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DFPGNVRELQNLMERAVLLANGRPVEPRHFLLEDDDWPLFEDDSADFAPEHSGAAAVPDF
CCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
SGGVALAADTSPRSAAAGGGPAVLPSAGGMPGAVIPLHEMERIMILKGLEATSGNRTQAA
CCCEEEEECCCCCCCCCCCCCEECCCCCCCCCCEECHHHHHHHHHEECCCCCCCCCCHHH
DLLGISVRTLRNKLNEYRAAGHPVD
HHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2315326; 11259647 [H]