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The map label for this gene is nifA [H]

Identifier: 220905633

GI number: 220905633

Start: 2861463

End: 2863097

Strand: Reverse

Name: nifA [H]

Synonym: Ddes_2373

Alternate gene names: 220905633

Gene position: 2863097-2861463 (Counterclockwise)

Preceding gene: 220905641

Following gene: 220905632

Centisome position: 99.64

GC content: 58.72

Gene sequence:

>1635_bases
ATGCCATATGAACTTTCGGCCAGCGGGCTAAACAATCCTTCGCAGACGGTAGGTCAGGTAGTACACCATCTGGCGCGGCT
TGTGGTGGGCAAGATGGACCGCAATGCCTTTTTCCGTATGCTTTCAAAACAGCTTCGTGTTTTATTTCACTATGACCGTT
TCTGCATTAACCTGTATGATGCGGAAAGGGAGTTTCTCAACCTGTTCACCGCAGCCGACGGCACGGTGGTAGAGTCTCTC
TCCAATACGCGCATTGCCCAGAACACGGTGGCCGGACTGGCCATTGCCTCACGTAAGCCCGTTGTCATCAACGATCTGGC
AAAACACGATTTCGGCGATTCGCCCATGCCTCTTTCCACCGTGGGCCTTAATTCCACCATCGCCCTGCCCCTCATCATCA
ACGGCGAGGTCATCGGCACACTGCACGTGTCTTTCATGCAGCCGCCCGAAAATATCGTACAGATACTCAATGTGCTGCTG
GAACTAAGCCCCGTGCTTTCCACATTTCTTTTTGCCGTGCTTTCCGAAGAGCGGCTTGAAAAAATGCGCGCGGCCCAGGC
TGGTTCCCCGCGCAGCCCGGAGGCAGATGTCACCGGCAGCATCTTGCTGGCCTCAAGCCTGCTGGAAACGCCGGACATGA
AAAAGCTCATGGCGCTGGCCCGCAAGGTGGCAAAGCTGCATATCCCCGTCATGATCCTGGGCGAAACGGGGACGGGCAAG
AGCATGTTGGCCCGCTGGCTGCACTTGCACAGCCCCAGGCGCAAGAACAGTTTTGTAAAGGTCAACTGCCCTTCCCTGCC
TCCCACGCTTTTTGAGAGTGAAATGTTCGGTTATGCCAAGGGGGCGTTCACCGGGGCTACCAGCAAGCGGGTGGGACGGG
TGGAACTGGCCCAGGGGGGCACGCTTTTTCTGGACGAGATAGGGGAACTCGCGCCGGAGATGCAGAGCAAGCTTTTGCAG
GTGCTGGAGGAAAATTCTTTTGAGCGTGTGGGCGAGGCCGCGCCCATCGGCGTGGATATGCGCGTCATGTCTGCCACCAA
TATTGACATGGCCGAGGCAATGGATCAGGGCCGTCTGCGGCGCGACCTGTACTACCGTCTTGGTTCGGTGGTATTGCGCA
TGCCGCCCCTGAGGGAACGCAGAAACGACATCCCGCTGTTTGTGGAGCATTTTGTCAGGCAGTTTTCCAGAGAGTACGAA
ATTCACCCGCCCCGGCTTTCACGTCCGGTTATGGATGTCCTGCAGGAACACGTCTGGCCGGGGAATATACGCGAACTGCG
CAATGTGGTGAGCCGCCTTCTGCTCCAGACCCTGGACGGGCAGATAACCGGCAGTTTTGTGGATGAAGCCCTGCACCTGT
GGAAAGGCCCGGCCGGAACAGCGCCGCCCGCTCCCGCAGCGCCGGCGTCCGGCATTGTTGCGGGTAATGCCGCAAACAGT
CCCCCTGTGCCTGCCCCCGCCCGGCAGGAAGCCCCGCCCGGCCTTCTTTCGCTTGAGGACAATGAGAGAGCGCATATAGA
AAAAGCCCTGCGTCTTTGCGGCGGCAAGCTGTCCGGTCCTGGCGGCGCGGCGGATATGCTCGGTATTCCGCGCTCGACGT
TGCAGCACCGCATCCGCAAGCTTGGCATCAGATAG

Upstream 100 bases:

>100_bases
TTTTCACAATATTGTTTGATTGATCAGTCTGTTTTGATTAAATGTGGGCATAATGCTTGTTTTTGGGCAGGAGAATGCCT
AATGTTGGGCAGGAGGATTT

Downstream 100 bases:

>100_bases
CCGCGCGGGCGGCCCGCGCGCAGGCCTTCTGGCCGGAATCGCCGGAGTGTGCCGCCCCGGACAGAAGGCAGGCCTCCATC
TGGCCTGCTCCTGTCGGACG

Product: Fis family sigma-54 specific transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MPYELSASGLNNPSQTVGQVVHHLARLVVGKMDRNAFFRMLSKQLRVLFHYDRFCINLYDAEREFLNLFTAADGTVVESL
SNTRIAQNTVAGLAIASRKPVVINDLAKHDFGDSPMPLSTVGLNSTIALPLIINGEVIGTLHVSFMQPPENIVQILNVLL
ELSPVLSTFLFAVLSEERLEKMRAAQAGSPRSPEADVTGSILLASSLLETPDMKKLMALARKVAKLHIPVMILGETGTGK
SMLARWLHLHSPRRKNSFVKVNCPSLPPTLFESEMFGYAKGAFTGATSKRVGRVELAQGGTLFLDEIGELAPEMQSKLLQ
VLEENSFERVGEAAPIGVDMRVMSATNIDMAEAMDQGRLRRDLYYRLGSVVLRMPPLRERRNDIPLFVEHFVRQFSREYE
IHPPRLSRPVMDVLQEHVWPGNIRELRNVVSRLLLQTLDGQITGSFVDEALHLWKGPAGTAPPAPAAPASGIVAGNAANS
PPVPAPARQEAPPGLLSLEDNERAHIEKALRLCGGKLSGPGGAADMLGIPRSTLQHRIRKLGIR

Sequences:

>Translated_544_residues
MPYELSASGLNNPSQTVGQVVHHLARLVVGKMDRNAFFRMLSKQLRVLFHYDRFCINLYDAEREFLNLFTAADGTVVESL
SNTRIAQNTVAGLAIASRKPVVINDLAKHDFGDSPMPLSTVGLNSTIALPLIINGEVIGTLHVSFMQPPENIVQILNVLL
ELSPVLSTFLFAVLSEERLEKMRAAQAGSPRSPEADVTGSILLASSLLETPDMKKLMALARKVAKLHIPVMILGETGTGK
SMLARWLHLHSPRRKNSFVKVNCPSLPPTLFESEMFGYAKGAFTGATSKRVGRVELAQGGTLFLDEIGELAPEMQSKLLQ
VLEENSFERVGEAAPIGVDMRVMSATNIDMAEAMDQGRLRRDLYYRLGSVVLRMPPLRERRNDIPLFVEHFVRQFSREYE
IHPPRLSRPVMDVLQEHVWPGNIRELRNVVSRLLLQTLDGQITGSFVDEALHLWKGPAGTAPPAPAAPASGIVAGNAANS
PPVPAPARQEAPPGLLSLEDNERAHIEKALRLCGGKLSGPGGAADMLGIPRSTLQHRIRKLGIR
>Mature_543_residues
PYELSASGLNNPSQTVGQVVHHLARLVVGKMDRNAFFRMLSKQLRVLFHYDRFCINLYDAEREFLNLFTAADGTVVESLS
NTRIAQNTVAGLAIASRKPVVINDLAKHDFGDSPMPLSTVGLNSTIALPLIINGEVIGTLHVSFMQPPENIVQILNVLLE
LSPVLSTFLFAVLSEERLEKMRAAQAGSPRSPEADVTGSILLASSLLETPDMKKLMALARKVAKLHIPVMILGETGTGKS
MLARWLHLHSPRRKNSFVKVNCPSLPPTLFESEMFGYAKGAFTGATSKRVGRVELAQGGTLFLDEIGELAPEMQSKLLQV
LEENSFERVGEAAPIGVDMRVMSATNIDMAEAMDQGRLRRDLYYRLGSVVLRMPPLRERRNDIPLFVEHFVRQFSREYEI
HPPRLSRPVMDVLQEHVWPGNIRELRNVVSRLLLQTLDGQITGSFVDEALHLWKGPAGTAPPAPAAPASGIVAGNAANSP
PVPAPARQEAPPGLLSLEDNERAHIEKALRLCGGKLSGPGGAADMLGIPRSTLQHRIRKLGIR

Specific function: Required for activation of most nif operons, which are directly involved in nitrogen fixation [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=337, Percent_Identity=39.1691394658754, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI1789087, Length=531, Percent_Identity=29.7551789077213, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI1790437, Length=332, Percent_Identity=38.2530120481928, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1788550, Length=340, Percent_Identity=37.6470588235294, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI1790299, Length=360, Percent_Identity=35.8333333333333, Blast_Score=208, Evalue=6e-55,
Organism=Escherichia coli, GI1788905, Length=243, Percent_Identity=42.798353909465, Blast_Score=205, Evalue=6e-54,
Organism=Escherichia coli, GI1789233, Length=333, Percent_Identity=35.4354354354354, Blast_Score=202, Evalue=6e-53,
Organism=Escherichia coli, GI87082152, Length=452, Percent_Identity=34.070796460177, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1787583, Length=231, Percent_Identity=41.991341991342, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1786524, Length=247, Percent_Identity=42.914979757085, Blast_Score=183, Evalue=3e-47,
Organism=Escherichia coli, GI87081872, Length=212, Percent_Identity=45.2830188679245, Blast_Score=181, Evalue=7e-47,
Organism=Escherichia coli, GI87081858, Length=398, Percent_Identity=29.1457286432161, Blast_Score=152, Evalue=6e-38,
Organism=Escherichia coli, GI1789828, Length=256, Percent_Identity=33.984375, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR010113
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 59634; Mature: 59503

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYELSASGLNNPSQTVGQVVHHLARLVVGKMDRNAFFRMLSKQLRVLFHYDRFCINLYD
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHEEHHH
AEREFLNLFTAADGTVVESLSNTRIAQNTVAGLAIASRKPVVINDLAKHDFGDSPMPLST
HHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHEECCCCCEEEHHHHHCCCCCCCCCEEE
VGLNSTIALPLIINGEVIGTLHVSFMQPPENIVQILNVLLELSPVLSTFLFAVLSEERLE
ECCCCEEEEEEEECCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMRAAQAGSPRSPEADVTGSILLASSLLETPDMKKLMALARKVAKLHIPVMILGETGTGK
HHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCH
SMLARWLHLHSPRRKNSFVKVNCPSLPPTLFESEMFGYAKGAFTGATSKRVGRVELAQGG
HHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCC
TLFLDEIGELAPEMQSKLLQVLEENSFERVGEAAPIGVDMRVMSATNIDMAEAMDQGRLR
EEHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHH
RDLYYRLGSVVLRMPPLRERRNDIPLFVEHFVRQFSREYEIHPPRLSRPVMDVLQEHVWP
HHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCC
GNIRELRNVVSRLLLQTLDGQITGSFVDEALHLWKGPAGTAPPAPAAPASGIVAGNAANS
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCC
PPVPAPARQEAPPGLLSLEDNERAHIEKALRLCGGKLSGPGGAADMLGIPRSTLQHRIRK
CCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHH
LGIR
HCCC
>Mature Secondary Structure 
PYELSASGLNNPSQTVGQVVHHLARLVVGKMDRNAFFRMLSKQLRVLFHYDRFCINLYD
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHEEHHH
AEREFLNLFTAADGTVVESLSNTRIAQNTVAGLAIASRKPVVINDLAKHDFGDSPMPLST
HHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHEECCCCCEEEHHHHHCCCCCCCCCEEE
VGLNSTIALPLIINGEVIGTLHVSFMQPPENIVQILNVLLELSPVLSTFLFAVLSEERLE
ECCCCEEEEEEEECCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMRAAQAGSPRSPEADVTGSILLASSLLETPDMKKLMALARKVAKLHIPVMILGETGTGK
HHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCH
SMLARWLHLHSPRRKNSFVKVNCPSLPPTLFESEMFGYAKGAFTGATSKRVGRVELAQGG
HHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCC
TLFLDEIGELAPEMQSKLLQVLEENSFERVGEAAPIGVDMRVMSATNIDMAEAMDQGRLR
EEHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHH
RDLYYRLGSVVLRMPPLRERRNDIPLFVEHFVRQFSREYEIHPPRLSRPVMDVLQEHVWP
HHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCC
GNIRELRNVVSRLLLQTLDGQITGSFVDEALHLWKGPAGTAPPAPAAPASGIVAGNAANS
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCC
PPVPAPARQEAPPGLLSLEDNERAHIEKALRLCGGKLSGPGGAADMLGIPRSTLQHRIRK
CCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHH
LGIR
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2664425; 3186446 [H]