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The map label for this gene is pleD [H]

Identifier: 220905630

GI number: 220905630

Start: 2855428

End: 2857092

Strand: Reverse

Name: pleD [H]

Synonym: Ddes_2370

Alternate gene names: 220905630

Gene position: 2857092-2855428 (Counterclockwise)

Preceding gene: 220905631

Following gene: 220905629

Centisome position: 99.43

GC content: 52.31

Gene sequence:

>1665_bases
ATGGAAAAACTGGCAAAAAGAAAAATTCGTATTTTTTATTATGTTACCGGGTTGTTGGCACTGATATTGATGTCAGTGGC
CCTGGCGATCCCGTACTTTATTTCTGTGCGCACCCAGTTTGACAGCATGATCGAGCAATCAAAGGTCCGTATTCTTGCAG
AAAAAAAGCAGACCCTTGTGGAAAGGGTGGAAAGGGTCAGCGTAGAGATCAGCGCCATGCGCGCCACCGTATATGCGGAA
TACCAGGCCATGGCCAAGGCATTGAGCGCCATGCTCGGCACTCTGGACATGAAGCCTGAAAAGATCATTGATGCCTTTGA
TCCCCAGGTGACCAGGCGTACCACACGGTTTGCCCTGCATATGGCGGGCATGGGGGTGGCCGTTTTTAACAGGGCTACCA
GCGAGCTTCTGTGGACGGATGAAAAAGATCTCGCCCATGTACTGCGTGAGCGACTTGTGGAGGGGCGGGTCGAGCCTTCG
GCGGATTTTCCTGTATGGGCCGTGGGGCAGCTCGGAGGCGCCCAGGAAGTTGTTGTTTTTGGCACGGACTGGGCTGTGGA
TGAGATAGCCAAAAGCCGCTGCAAGGCCATTATCCGCACCCTGCGCTTTCCTCTTAACCAGTATGTATGGATCAATGAGG
TGCGTAACTACAGCGGCGGCCCGGGCTATGCCGTGCGGGCCGTGCATGTTGCCGAGCCGGAAACCGAGGGCATGCCCCTG
TCCACAGACATGGCGGACATCAGGGGCAACCTGCCGTACAAGGAAGAACTGGAAGGCGTTGTCGCGCACGGCGACATATT
TTTTACCTACTATTTCAAAAAGCCGGATTCTGACGAAATAAGCGAAAAACTCACGTATGCCAAACTCTACAAACCGTTTA
ACTGGATTGTATGCACGGGCATCTATACAAACGATATGGAAGTCATCATACAGAAGCGCGAGGACGACTTCCGCAGTGCA
TTCAAGGACCAGATTATTTCCTTTGCCAGAATAATTTCCATCATGGCCCTGCTGTACGCCGTTTTGATGATTTTTTTCGA
GCGCCACCTTACGGGCATGGTCAACGGCTTTATTCTCAAGCTCAAGGATGACGAAACCGCCTTGCGGGAAGAAAAAAACA
AGCTTGATGATGCCTACAAGGAACTGGAACGTGTTGCCTATTATGACTTTCTTACAGGTCTGCTCAACCGGCGGGCCATG
TATGAACGTCTTGAAAAGGAATTTTCGCGCGCGCAGCGCGAGGGGCTGCAGTTCTGCCTGGTGCTGGCCGATATCGACCA
TTTCAAGGTTGTGAACGATACTTATGGACATGATGCGGGCGATGTGGTGCTGCAATCCATCGCGGAAATTTTTCAGAACA
ATATACGGCAGGAAGACTGCGCCTCCCGCTGGGGCGGCGAGGAATTTCTGATTCTGGCAATTTCCTGCGGGCTTGAAGAC
GGGCTGTCGCTGGCGGAAAAGCTGCGCAAGGCCGTTGAGGATACTCCCATCCGCATCGGAGCCGCAATTATCAATGTCAC
CATCACGCTGGGCGTGGTAGAGTACACCAAGGGCAAATCCTTCAGCACGCTTATCAAAGAGGCGGATTTTTATCTTTACG
AGGGCAAGCGCCAGAGAAACTGTGTCATGTCCAGTTCCGGCACAGCCAAGGAACCCTTAGAGTAA

Upstream 100 bases:

>100_bases
TTCCTATGTACAGATTATTGGCTATACTGTCTGTGCCTCCAGAACATTCTGAAAGATTTGTCTGGAGCCTCCAATCTGCC
GTCTCCGGCAAAGACACGTC

Downstream 100 bases:

>100_bases
TTACCGCTTAAAATGATTGCTTAACGGTCGGCAAAGCCGCCTTACAGTCATGTGGCGACAAAGCCTTGGCGAGCAGCTCT
GGTGTGAAATGTTCTACATC

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MEKLAKRKIRIFYYVTGLLALILMSVALAIPYFISVRTQFDSMIEQSKVRILAEKKQTLVERVERVSVEISAMRATVYAE
YQAMAKALSAMLGTLDMKPEKIIDAFDPQVTRRTTRFALHMAGMGVAVFNRATSELLWTDEKDLAHVLRERLVEGRVEPS
ADFPVWAVGQLGGAQEVVVFGTDWAVDEIAKSRCKAIIRTLRFPLNQYVWINEVRNYSGGPGYAVRAVHVAEPETEGMPL
STDMADIRGNLPYKEELEGVVAHGDIFFTYYFKKPDSDEISEKLTYAKLYKPFNWIVCTGIYTNDMEVIIQKREDDFRSA
FKDQIISFARIISIMALLYAVLMIFFERHLTGMVNGFILKLKDDETALREEKNKLDDAYKELERVAYYDFLTGLLNRRAM
YERLEKEFSRAQREGLQFCLVLADIDHFKVVNDTYGHDAGDVVLQSIAEIFQNNIRQEDCASRWGGEEFLILAISCGLED
GLSLAEKLRKAVEDTPIRIGAAIINVTITLGVVEYTKGKSFSTLIKEADFYLYEGKRQRNCVMSSSGTAKEPLE

Sequences:

>Translated_554_residues
MEKLAKRKIRIFYYVTGLLALILMSVALAIPYFISVRTQFDSMIEQSKVRILAEKKQTLVERVERVSVEISAMRATVYAE
YQAMAKALSAMLGTLDMKPEKIIDAFDPQVTRRTTRFALHMAGMGVAVFNRATSELLWTDEKDLAHVLRERLVEGRVEPS
ADFPVWAVGQLGGAQEVVVFGTDWAVDEIAKSRCKAIIRTLRFPLNQYVWINEVRNYSGGPGYAVRAVHVAEPETEGMPL
STDMADIRGNLPYKEELEGVVAHGDIFFTYYFKKPDSDEISEKLTYAKLYKPFNWIVCTGIYTNDMEVIIQKREDDFRSA
FKDQIISFARIISIMALLYAVLMIFFERHLTGMVNGFILKLKDDETALREEKNKLDDAYKELERVAYYDFLTGLLNRRAM
YERLEKEFSRAQREGLQFCLVLADIDHFKVVNDTYGHDAGDVVLQSIAEIFQNNIRQEDCASRWGGEEFLILAISCGLED
GLSLAEKLRKAVEDTPIRIGAAIINVTITLGVVEYTKGKSFSTLIKEADFYLYEGKRQRNCVMSSSGTAKEPLE
>Mature_554_residues
MEKLAKRKIRIFYYVTGLLALILMSVALAIPYFISVRTQFDSMIEQSKVRILAEKKQTLVERVERVSVEISAMRATVYAE
YQAMAKALSAMLGTLDMKPEKIIDAFDPQVTRRTTRFALHMAGMGVAVFNRATSELLWTDEKDLAHVLRERLVEGRVEPS
ADFPVWAVGQLGGAQEVVVFGTDWAVDEIAKSRCKAIIRTLRFPLNQYVWINEVRNYSGGPGYAVRAVHVAEPETEGMPL
STDMADIRGNLPYKEELEGVVAHGDIFFTYYFKKPDSDEISEKLTYAKLYKPFNWIVCTGIYTNDMEVIIQKREDDFRSA
FKDQIISFARIISIMALLYAVLMIFFERHLTGMVNGFILKLKDDETALREEKNKLDDAYKELERVAYYDFLTGLLNRRAM
YERLEKEFSRAQREGLQFCLVLADIDHFKVVNDTYGHDAGDVVLQSIAEIFQNNIRQEDCASRWGGEEFLILAISCGLED
GLSLAEKLRKAVEDTPIRIGAAIINVTITLGVVEYTKGKSFSTLIKEADFYLYEGKRQRNCVMSSSGTAKEPLE

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=167, Percent_Identity=36.5269461077844, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI145693134, Length=156, Percent_Identity=34.6153846153846, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI1787802, Length=172, Percent_Identity=34.8837209302326, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1787816, Length=156, Percent_Identity=37.1794871794872, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1787262, Length=212, Percent_Identity=31.1320754716981, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87082007, Length=168, Percent_Identity=34.5238095238095, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1786584, Length=170, Percent_Identity=28.2352941176471, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1788085, Length=175, Percent_Identity=33.1428571428571, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI87081977, Length=167, Percent_Identity=28.7425149700599, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI87081974, Length=163, Percent_Identity=26.3803680981595, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1787541, Length=171, Percent_Identity=30.4093567251462, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788381, Length=115, Percent_Identity=33.9130434782609, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 63046; Mature: 63046

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLAKRKIRIFYYVTGLLALILMSVALAIPYFISVRTQFDSMIEQSKVRILAEKKQTLV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERVERVSVEISAMRATVYAEYQAMAKALSAMLGTLDMKPEKIIDAFDPQVTRRTTRFALH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHH
MAGMGVAVFNRATSELLWTDEKDLAHVLRERLVEGRVEPSADFPVWAVGQLGGAQEVVVF
HHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCEEEEE
GTDWAVDEIAKSRCKAIIRTLRFPLNQYVWINEVRNYSGGPGYAVRAVHVAEPETEGMPL
ECCCCHHHHHHHHHHHHHHHHHCCHHCEEEHHHHHCCCCCCCCEEEEEEECCCCCCCCCC
STDMADIRGNLPYKEELEGVVAHGDIFFTYYFKKPDSDEISEKLTYAKLYKPFNWIVCTG
CCCHHHHCCCCCCHHHHCCEEEECEEEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEE
IYTNDMEVIIQKREDDFRSAFKDQIISFARIISIMALLYAVLMIFFERHLTGMVNGFILK
EEECCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
LKDDETALREEKNKLDDAYKELERVAYYDFLTGLLNRRAMYERLEKEFSRAQREGLQFCL
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEE
VLADIDHFKVVNDTYGHDAGDVVLQSIAEIFQNNIRQEDCASRWGGEEFLILAISCGLED
EEECCCHHEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEEEECCCCC
GLSLAEKLRKAVEDTPIRIGAAIINVTITLGVVEYTKGKSFSTLIKEADFYLYEGKRQRN
HHHHHHHHHHHHCCCCCEEEHHHEEEEEEEEEEEECCCCHHHHHHHHCCCEEECCCCCCC
CVMSSSGTAKEPLE
EEECCCCCCCCCCC
>Mature Secondary Structure
MEKLAKRKIRIFYYVTGLLALILMSVALAIPYFISVRTQFDSMIEQSKVRILAEKKQTLV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERVERVSVEISAMRATVYAEYQAMAKALSAMLGTLDMKPEKIIDAFDPQVTRRTTRFALH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHH
MAGMGVAVFNRATSELLWTDEKDLAHVLRERLVEGRVEPSADFPVWAVGQLGGAQEVVVF
HHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCEEEEE
GTDWAVDEIAKSRCKAIIRTLRFPLNQYVWINEVRNYSGGPGYAVRAVHVAEPETEGMPL
ECCCCHHHHHHHHHHHHHHHHHCCHHCEEEHHHHHCCCCCCCCEEEEEEECCCCCCCCCC
STDMADIRGNLPYKEELEGVVAHGDIFFTYYFKKPDSDEISEKLTYAKLYKPFNWIVCTG
CCCHHHHCCCCCCHHHHCCEEEECEEEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEE
IYTNDMEVIIQKREDDFRSAFKDQIISFARIISIMALLYAVLMIFFERHLTGMVNGFILK
EEECCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEE
LKDDETALREEKNKLDDAYKELERVAYYDFLTGLLNRRAMYERLEKEFSRAQREGLQFCL
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEE
VLADIDHFKVVNDTYGHDAGDVVLQSIAEIFQNNIRQEDCASRWGGEEFLILAISCGLED
EEECCCHHEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEEEECCCCC
GLSLAEKLRKAVEDTPIRIGAAIINVTITLGVVEYTKGKSFSTLIKEADFYLYEGKRQRN
HHHHHHHHHHHHCCCCCEEEHHHEEEEEEEEEEEECCCCHHHHHHHHCCCEEECCCCCCC
CVMSSSGTAKEPLE
EEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA