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The map label for this gene is rsmF [H]

Identifier: 220905585

GI number: 220905585

Start: 2795499

End: 2796887

Strand: Direct

Name: rsmF [H]

Synonym: Ddes_2325

Alternate gene names: 220905585

Gene position: 2795499-2796887 (Clockwise)

Preceding gene: 220905581

Following gene: 220905587

Centisome position: 97.29

GC content: 63.93

Gene sequence:

>1389_bases
ATGCCGCCCATGCAAAAAACTGCTGCCCGTTCGTTCCGTATTGTCTGCAAAAACACGCACACCGCCTCGGTGGAAGCCCT
GCTGCGCGCCCAGGGATATGAGTTTGAAGCCGAGCCTTTTTCTCCCCTGTGCCGCCGCCTTGTGGCGGAGCCGCGCCCGC
TGGGCAGTTCGCTGGCAGCTTTTTTCGGCTACATCTATATCCAGGACCGTTCCTCCATGCTGCCGCCTCTGGCGCTGGCT
CCGGCGGCAGGCTCGGCCGTACTGGACATGTGCGCCAGCCCCGGCAGCAAAACGGGTTTTCTGGCCCAACTTGTGGGCCG
CACCGGCTTTGTGCTCGGCAACGAGCCTTCCCCGTCGCGGCTGGCGACACTGCGGGCCAACCTGCACCAATGCGGCTTCA
TCCATACAGGAACCTGCTCCTACAGCGGCGACGCCCTGCCCCTGCGCCCCGGCTCGTGGAAAAACATCCTGCTGGACCCT
CCCTGTTCGGGCTGGGGAACAGTGGAAAAAAATCCACAGGTGCTCAAGCTCTGGCAGGGCGACAAGCTCGACAGCCTCAC
AAGCCTGCAACGCCGTCTGCTGCGACATGCAGAACGCCTGCTGGCACCCGGCGGGCACGTGGTTTTTTCCACCTGCACCA
CCAATGTGGCAGAAAATGAAGATCAGGTACGCTTTGCCGAAGAACATCTGGGGCTTGTGCGTGAACACATCAACCCTTTC
CCCGGCTTTGTGTGGGAAGAGCTGCCCGGCGGCGAAGGCACATTGCGGGTAGACGGCGCGCGCTCCCAGGCCCAGGGTTT
TTATGTGGCGCTTTTGCGCAAGCCCGAAAATGCGTCTTCCCATCACAGAGAACCGAAGGCTGAGCAGGCGACGCATTTCG
GGCCTGCCCGCGGTTCCGACGGAGAGACAGCAGGGGCTTTTCTGAACGGAGGAAGAGCGCGCAGCAAAAACGGCAGCCGT
CCGGATGCGCGTACGGGCAGCCGTGTTCCGGCAGGCACCCCCCTGCCCCGGTCATGCCTTCACGGTCCCACGTGCGATCC
GGACATGCTGCCGCCGGGACAGGCCATGCTGTACGGCGAACATGTCCGCTTCATCCCGGAACAGGCCGCCGCACTACTGC
CGCCGGACTGCGCCTGGCAAGGCGCGCTGCTGGGCAAGTTCAACGGCGGCAAGCTGGATGCGGCCCCGCGCCTGCGCGCC
CTCATGACGCCGCAGCCTGACCGGGAAAAGAGCCTTGTTCTTGAAGATATTTCTGATATTTCGGCCCTGCTTAGCGGACA
GAGCCGCCAGACGGGCCTTCAGGGCCGCGAGGCGGCGCTGTGGTGGCGCGACCTGCCCTTGGGGCGGGTGGGCCTCAAAC
AGGGCCGCGCTCTGACGGGATTCAAATAG

Upstream 100 bases:

>100_bases
CGCACTTGACGCGCCGCGCAATATCTAAGCCGGGGCAGTCGCCACAGGCGCGGACAAAGGCATAAAAAAGCGCTTGCGGC
TGAAACCACATCTCCATATT

Downstream 100 bases:

>100_bases
CGCACTGTCACGTTGAGGATATCATCCTCAACGTGGTATGCCGCTCACGGTGACGCTCAGAAGCGCAACTGTCAAACGCT
GCCAAACACCACAGCTTTTT

Product: Fmu (Sun) domain-containing protein

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MPPMQKTAARSFRIVCKNTHTASVEALLRAQGYEFEAEPFSPLCRRLVAEPRPLGSSLAAFFGYIYIQDRSSMLPPLALA
PAAGSAVLDMCASPGSKTGFLAQLVGRTGFVLGNEPSPSRLATLRANLHQCGFIHTGTCSYSGDALPLRPGSWKNILLDP
PCSGWGTVEKNPQVLKLWQGDKLDSLTSLQRRLLRHAERLLAPGGHVVFSTCTTNVAENEDQVRFAEEHLGLVREHINPF
PGFVWEELPGGEGTLRVDGARSQAQGFYVALLRKPENASSHHREPKAEQATHFGPARGSDGETAGAFLNGGRARSKNGSR
PDARTGSRVPAGTPLPRSCLHGPTCDPDMLPPGQAMLYGEHVRFIPEQAAALLPPDCAWQGALLGKFNGGKLDAAPRLRA
LMTPQPDREKSLVLEDISDISALLSGQSRQTGLQGREAALWWRDLPLGRVGLKQGRALTGFK

Sequences:

>Translated_462_residues
MPPMQKTAARSFRIVCKNTHTASVEALLRAQGYEFEAEPFSPLCRRLVAEPRPLGSSLAAFFGYIYIQDRSSMLPPLALA
PAAGSAVLDMCASPGSKTGFLAQLVGRTGFVLGNEPSPSRLATLRANLHQCGFIHTGTCSYSGDALPLRPGSWKNILLDP
PCSGWGTVEKNPQVLKLWQGDKLDSLTSLQRRLLRHAERLLAPGGHVVFSTCTTNVAENEDQVRFAEEHLGLVREHINPF
PGFVWEELPGGEGTLRVDGARSQAQGFYVALLRKPENASSHHREPKAEQATHFGPARGSDGETAGAFLNGGRARSKNGSR
PDARTGSRVPAGTPLPRSCLHGPTCDPDMLPPGQAMLYGEHVRFIPEQAAALLPPDCAWQGALLGKFNGGKLDAAPRLRA
LMTPQPDREKSLVLEDISDISALLSGQSRQTGLQGREAALWWRDLPLGRVGLKQGRALTGFK
>Mature_461_residues
PPMQKTAARSFRIVCKNTHTASVEALLRAQGYEFEAEPFSPLCRRLVAEPRPLGSSLAAFFGYIYIQDRSSMLPPLALAP
AAGSAVLDMCASPGSKTGFLAQLVGRTGFVLGNEPSPSRLATLRANLHQCGFIHTGTCSYSGDALPLRPGSWKNILLDPP
CSGWGTVEKNPQVLKLWQGDKLDSLTSLQRRLLRHAERLLAPGGHVVFSTCTTNVAENEDQVRFAEEHLGLVREHINPFP
GFVWEELPGGEGTLRVDGARSQAQGFYVALLRKPENASSHHREPKAEQATHFGPARGSDGETAGAFLNGGRARSKNGSRP
DARTGSRVPAGTPLPRSCLHGPTCDPDMLPPGQAMLYGEHVRFIPEQAAALLPPDCAWQGALLGKFNGGKLDAAPRLRAL
MTPQPDREKSLVLEDISDISALLSGQSRQTGLQGREAALWWRDLPLGRVGLKQGRALTGFK

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=250, Percent_Identity=32.4, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI76150623, Length=250, Percent_Identity=32.4, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI32698918, Length=193, Percent_Identity=33.6787564766839, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI23199998, Length=228, Percent_Identity=33.3333333333333, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI270288818, Length=228, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI270288816, Length=228, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI8922322, Length=228, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI301336155, Length=207, Percent_Identity=30.9178743961353, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI39995082, Length=207, Percent_Identity=30.9178743961353, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI40316918, Length=177, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI11545785, Length=170, Percent_Identity=34.7058823529412, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI87081985, Length=286, Percent_Identity=35.6643356643357, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI2367212, Length=171, Percent_Identity=29.2397660818713, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17536757, Length=286, Percent_Identity=30.4195804195804, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI71998419, Length=229, Percent_Identity=30.5676855895196, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI115534021, Length=231, Percent_Identity=30.7359307359307, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI25143471, Length=200, Percent_Identity=30.5, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324268, Length=179, Percent_Identity=35.195530726257, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6319447, Length=293, Percent_Identity=28.3276450511945, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI22024126, Length=255, Percent_Identity=29.8039215686275, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21355201, Length=179, Percent_Identity=33.5195530726257, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24668781, Length=231, Percent_Identity=31.6017316017316, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21356513, Length=231, Percent_Identity=31.6017316017316, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21356579, Length=165, Percent_Identity=30.3030303030303, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19920494, Length=172, Percent_Identity=31.3953488372093, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR011023 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu [H]

EC number: =2.1.1.178 [H]

Molecular weight: Translated: 49878; Mature: 49747

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPMQKTAARSFRIVCKNTHTASVEALLRAQGYEFEAEPFSPLCRRLVAEPRPLGSSLAA
CCCCHHHHHCCEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCHHHHHH
FFGYIYIQDRSSMLPPLALAPAAGSAVLDMCASPGSKTGFLAQLVGRTGFVLGNEPSPSR
HHEEEEEECCCCCCCCEEECCCCCHHHHHHHCCCCCCHHHHHHHHCCCCEEECCCCCHHH
LATLRANLHQCGFIHTGTCSYSGDALPLRPGSWKNILLDPPCSGWGTVEKNPQVLKLWQG
HHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEECC
DKLDSLTSLQRRLLRHAERLLAPGGHVVFSTCTTNVAENEDQVRFAEEHLGLVREHINPF
CCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCC
PGFVWEELPGGEGTLRVDGARSQAQGFYVALLRKPENASSHHREPKAEQATHFGPARGSD
CCCHHHHCCCCCCEEEECCCCCCCCCEEEEEEECCCCCCHHCCCCCHHHHHCCCCCCCCC
GETAGAFLNGGRARSKNGSRPDARTGSRVPAGTPLPRSCLHGPTCDPDMLPPGQAMLYGE
CCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCEEEECC
HVRFIPEQAAALLPPDCAWQGALLGKFNGGKLDAAPRLRALMTPQPDREKSLVLEDISDI
HHHHCHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHH
SALLSGQSRQTGLQGREAALWWRDLPLGRVGLKQGRALTGFK
HHHHCCCCHHCCCCCCCEEEEEECCCCCHHHHHCCCCCCCCC
>Mature Secondary Structure 
PPMQKTAARSFRIVCKNTHTASVEALLRAQGYEFEAEPFSPLCRRLVAEPRPLGSSLAA
CCCHHHHHCCEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCHHHHHH
FFGYIYIQDRSSMLPPLALAPAAGSAVLDMCASPGSKTGFLAQLVGRTGFVLGNEPSPSR
HHEEEEEECCCCCCCCEEECCCCCHHHHHHHCCCCCCHHHHHHHHCCCCEEECCCCCHHH
LATLRANLHQCGFIHTGTCSYSGDALPLRPGSWKNILLDPPCSGWGTVEKNPQVLKLWQG
HHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEECC
DKLDSLTSLQRRLLRHAERLLAPGGHVVFSTCTTNVAENEDQVRFAEEHLGLVREHINPF
CCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCC
PGFVWEELPGGEGTLRVDGARSQAQGFYVALLRKPENASSHHREPKAEQATHFGPARGSD
CCCHHHHCCCCCCEEEECCCCCCCCCEEEEEEECCCCCCHHCCCCCHHHHHCCCCCCCCC
GETAGAFLNGGRARSKNGSRPDARTGSRVPAGTPLPRSCLHGPTCDPDMLPPGQAMLYGE
CCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCEEEECC
HVRFIPEQAAALLPPDCAWQGALLGKFNGGKLDAAPRLRALMTPQPDREKSLVLEDISDI
HHHHCHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHH
SALLSGQSRQTGLQGREAALWWRDLPLGRVGLKQGRALTGFK
HHHHCCCCHHCCCCCCCEEEEEECCCCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA